AORTIC BARORECEPTORS PLAY A PREDOMINANT ROLE IN THE REGULATION OF HINDLIMB VASCULAR-RESISTANCE IN RATS

In previous studies using bilateral carotid occlusion in conscious freely moving rats we suggested that aortic baroreceptors may play a more important role in the regulation of hindlimb than in renal and mesenteric vascular resistances. In the present study we performed electrical stimulation of the aortic baroreceptor nerve and analyzed the changes in mean arterial pressure and in hindlimb, renal, and mesenteric vascular resistances. All the experiments were performed under urethan anesthesia. Unilateral electrical stimulation (3 V, 2 ms, 50 Hz) of the aortic baroreceptor nerve produced a fall in arterial pressure (-27 +/- 3 mmHg) and an important reduction in hindlimb vascular resistance (-43 +/- 5%), with an increase in renal (+3 +/- 14%) and mesenteric (+48 +/- 12%) vascular resistances. Similar changes in arterial pressure as well as in the resistance of the three vascular beds studied were also observed during electrical stimulation of the aortic baroreceptor nerve in rats with bilateral carotid baroreceptor denervation or in rats treated with methylatropine. The data obtained with electrical stimulation indicated that aortic baroreceptors play a more important role in the regulation of blood flow in hindlimb than in renal and mesenteric vascular beds.

Quantitative estimates of time-averaging in terebratulid brachiopod shell accumulations from a modern tropical shelf

Quantitative estimates of time-averaging in marine shell accumulations available to date are limited primarily to aragonitic mollusk shells. We assessed time-averaging in Holocene assemblages of calcitic brachiopod shells by direct dating of individual specimens of the terebratulid brachiopod Bouchardia rosea. The data were collected from exceptional (brachiopod-rich) shell assemblages, occurring surficially on a tropical mixed carbonate-siliciclastic shelf (the Southeast Brazilian Bight, SW Atlantic), a setting that provides a good climatic and environmental analog for many Paleozoic brachiopod shell beds of North America and Europe. A total of 82 individual brachiopod shells, collected from four shallow (5-25 m) nearshore (<2.5 km from the shore) localities, were dated by using amino acid racemization (D-alloisoleucine/L-isoleucine value) calibrated with five AMS-radiocarbon dates (r(2) = 0.933). This is the first study to demonstrate that amino acid racemization methods can provide accurate and precise ages for individual shells of calcitic brachiopods.The dated shells vary in age from modern to 3000 years, with a standard deviation of 690 years. The age distribution is strongly right-skewed: the young shells dominate the dated specimens and older shells are increasingly less common. However, the four localities display significant differences in the range of time-averaging and the form of the age distribution. The dated shells vary notably in the quality of preservation, but there is no significant correlation between taphonomic condition and age, either for individual shells or at assemblage level.These results demonstrate that fossil brachiopods may show considerable time-averaging, but the scale and nature of that mixing may vary greatly among sites. Moreover, taphonomic condition is not a reliable indicator of pre-burial history of individual brachiopod shells or the scale of temporal mixing within the entire assemblage. The results obtained for brachiopods are strikingly similar to results previously documented for mollusks and suggest that differences in mineralogy and shell microstructure are unlikely to be the primary factors controlling the nature and scale of time-averaging. Environmental factors and local fluctuations in populations of shell-producing organisms are more likely to be the principal determinants of time-averaging in marine benthic shelly assemblages. The long-term survival of brachiopod shells is incongruent with the rapid shell destruction observed in taphonomic experiments. The results support the taphonomic model that shells remain protected below (but perhaps near) the surface through their early taphonomic history. They may be brought back up to the surface intermittently by bioturbation and physical reworking, but only for short periods of time. This model explains the striking similarities in time-averaging among different types of organisms and the lack of correlation between time-since-death and shell taphonomy.

Predictors of incorrectness in antimicrobial prescription in the Bauru State Hospital (Bauru city, São Paulo State, Brazil)

The inappropriate use of antimicrobials in hospitals presents a negative impact on patient outcome and is associated with the emergence and spread of multidrug-resistant microorganisms. Antimicrobial stewardship programs (ASPs) have been instituted in order to improve the quality of prescriptions in hospitals. In this setting, the identification of patterns of inappropriate antimicrobial prescription is a valuable tool that allows ASPs to identify priorities for directing educative/restrictive policies. With this purpose, a study was conducted in the Bauru State Hospital, a teaching hospital with 285 beds affiliated to the Botucatu Medical School, São Paulo State University. The hospital maintains an active ASP since it was opened, in 2002. We selected 25% of the requests for parenteral antimicrobials (RPAs) from 2005 for analysis. Prescriptions for prophylactic purposes were excluded. All other RPAs were classified according to a modified Kunin and Jones categories. Univariate and multivariable analyses were performed to identify predictors of general inappropriateness and of specific prescription errors. Prescriptions classified as "appropriate'' or "probably appropriate" were selected as controls in all stages of the study. Among 963 RPAs included in our study, 34.6% were inappropriate. General predictors of inappropriateness were: prescription on weekends/holidays (OR = 1.67, 95% CI = 1.20-2.28, p = 0.002), patient from intensive care unit (OR = 1.57, 95% CI = 1.11-2.23, p = 0.01), peritoneal (OR = 2.15, 95% CI = 1.27-3.65, p = 0.004) or urinary tract infection (OR = 1.89, 95% CI = 1.25-2.87, p = 0.002), combined therapy with two or more antimicrobials (OR = 1.72, 95% CI = 1.15-2.57, p = 0.008) and prescriptions including penicillin (OR = 2.12, 95% CI = 1.39-3.25, p = 0.001) or first-generation cephalosporins (OR = 1.74, 95% CI = 1.01-3.00, p = 0.048). Previous consultation with an infectious diseases (ID) specialist had a protective effect against inappropriate prescription (OR = 0.34, 95% CI = 0.24-0.50, p < 0.001). Factors independently associated with specific prescription errors varied. However, consultation with an ID specialist was protective against both unnecessary antimicrobial use (OR = 0.04, 95% CI = 0.01-0.26, p = 0.001) and requests for agents with insufficient spectrum (OR = 0.14, 95% CI = 0.03-0.30, p = 0.01). In conclusion, the analysis of factors predictive of inappropriateness in antimicrobial prescription allowed us to identify issues requiring intervention. Also, it provided a positive feedback of the ASP efficacy, demonstrating the importance of previous consultation with an ID specialist to assure the quality of antimicrobial prescriptions.

Physa mezzalirai, a new cretaceous basommatophoran from Adamantina formation, Brazil.

This paper deals with a formal description of Physa mezzalirai (Basommatophora, Physidae), a new species of freshwater gastropod that lived in upper Cretaceous deposits, in sand-silty beds of Adamantina formation, Bauru basin, from Marlia, São Paulo state, Brazil. This formal description legitimates the availability of the name previously described in 2010 by same authors; being this paper the correct citation for the species name.

Investigation of the pore blockage of a Brazilian dolomite during the sulfation reaction

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aspectos fisico-geograficos da Regiao Administrativa de Aracatuba.

In this region there are exposures of sandstones, siltstones, and argillites of the Adamantina Formation, belonging to the Bauru Group. Using satellite and radar images, superposition of drainage maps, toposheets geologic and pedologic maps and field trips, it was possible to discern (three geomorphological domains; a) hilly and interfluvial plateau in the divisories areas of Tiete - Aguapei and Tiete - Sao Jose dos Dourados mainrivers; b) colluvial surfaces located around the interfluvial plateau; and c) fluvial terraces and floodplains. The indiscriminant use of the soils is accelerating the erosive process, increasing the sedimentation of the river beds, the size of the floodplains and prejudicing the water quality, used to supply the rural and urban population. -from English summary

Seqüência Ordovício-Siluriana e Devoniana no Flanco Norte da Bacia do Paraná

Two stratigraphic sequences characterize the basal units of the Paraná basin. The Ordovician-Silurian sequence overlie directly the Neoproterozoic basement and consists of a 55m-thick unit of coarse-grained sandstones, diamictites, fossiliferous shales and fine-grained micaceous sandstones. The Alto Garças Formation constitutes the base of the sequence and is made of coarse-grained, massive and reddish sandstones associated with conglomeratic lenses. Diamictites with pebbles of diverse composition in siltic and arenaceous matrix were deposited during the Ordovician-Silurian glaciation. Whenever the basal sandstones are absent, the diamictites directly overlie the basement. The diamictites were previously included in the Vila Maria Formation. However our study revealed that they are part of the Iapó Formation. A transgressive event following the glaciation is marked by the deposition of the Vila Maria Formation, which is characterized by fossiliferous (mollusks, brachiopods, cryptospores and microplankton) and laminated shales and siltstones, grading upward to fine-grained micaceous sandstones with hummocky cross stratification. Layers containing trace fossils (Anthrophycus) occur at the transition between the siltstones and the sandstones. The Devonian sequence is represented by 80-170 meters thick sandstones of the Furnas Formation (lower unit) and a sucession of sandstones, siltstones and shales of the Ponta Grossa Formation (upper unit). Unlike other areas of the Paraná Basin, the Ponta Grossa Formation is characterized by coarsening-upward succession beginning with fine sandstones and grading upward to coarse and very-coarse sandstone beds. Cretaceous modifying tectonics affected the Paleozoic sequences, which are cut by a series of faults, in some cases showing displacements greater than 500 meters.

Use of the HDS system (hydric depuration with soils) associated to other constructed wetlands techniques for public water supply

Two projects of water treatment for public water supply were developed and operated by using combined systems of constructed wetlands. One of the projects was carried out in the town of Analandia, Sao Paulo, Brazil and wetlands with floating aquatic plants associated to the HDS system were used. Nearly 6480 inhabitants were supplied. The other conducted project was an experimental station in partnership with SABESP (Sao Paulo State Sanitation Agency/Brazil), for the pretreatment of 1700 l.s-1 of waters from the Cotia River, which is used for the population's supply after conventional treatment at the Lower Cotia Water Treatment Station. For this pilot project, wetlands with emergents and floating plants associated to the HDS system were used. The proposed objectives were achieved in both projects.

Central nitric oxide modulates hindquarter vasodilation elicited by AMPA receptor stimulation in the NTS of conscious rats

Microinjection of S-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) in the nucleus of the solitary tract (NTS) of conscious rats causes hypertension, bradycardia, and vasoconstriction in the renal, mesenteric, and hindquarter vascular beds. In the hindquarter, the initial vasoconstriction is followed by vasodilation with AMPA doses >5 pmol/100 nl. To test the hypothesis that this vasodilation is caused by activation of a nitroxidergic pathway in the NTS, we examined the effect of pretreatment with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME, 10 nmol/100 nl, microinjected into the NTS) on changes in mean arterial pressure, heart rate, and regional vascular conductance (VC) induced by microinjection of AMPA (10 pmol/100 nl in the NTS) in conscious rats. AMPA increased hindquarter VC by 18 ± 4%, but after pretreatment with L-NAME, AMPA reduced hindquarter VC by 16 ± 7% and 17 ± 9% (5 and 15 min after pretreatment, P < 0.05 compared with before pretreatment). Pretreatment with L-NAME reduced AMPA-induced bradycardia from 122 ± 40 to 92 ± 32 beats/min but did not alter the hypertension induced by AMPA (35 ± 5 mmHg before pretreatment, 43 ± 6 mmHg after pretreatment). Control injections with D-NAME did not affect resting values or the response to AMPA. The present study shows that stimulation of AMPA receptors in the NTS activates both vasodilatatory and vasoconstrictor mechanisms and that the vasodilatatory mechanism depends on production of nitric oxide in the NTS. Copyright © 2006 the American Physiological Society.

Significado paleobiológico de agrupamentos (coloniais/ gregários) de Conularia quichua Ulrich, 1890 (Cnidaria), Formação Ponta Grossa, Devoniano (Pragiano-Emsiano), Bacia do Paraná, Brasil

The Conularia beds of the Ponta Grossa Formation (Devonian) of the Paraná Basin, southern Brazil, yield well-preserved specimens of Conularia quichua Ulrich and Paraconularia africana Sharpe. Many of these are preserved in life orientation. Also, one of the C. quichua specimens has five faces instead of four, providing additional evidence of a cnidarian affinity for conulariids. Conulariids occur in the Jaguariaíva Member (or Sequence B, transgressive system tract) containing several obrution deposits beneath marine flooding surfaces. Taphonomic data obtained from these beds show conclusively that both C. quichua and P. africana were epibenthic, sessile invertebrates originally oriented with their long axis perpendicular to the bottom and with their aperture opening upward. Of the 136 C. quichua specimens examined here, 125 occur isolated. Eleven of the C. quichua specimens collectively occur in five discrete clusters consisting of two or three specimens. All of the clustered specimens are fully inflated (exhibiting a rectangular transverse cross section) or slightly compressed longitudinally. In all of these specimens the apex is missing, and thus the problem of whether the clusters were clonal colonies or formed through preferential larval settlement cannot be resolved conclusively. However, in the single cluster consisting of three specimens, the specimens are oriented perpendicular to bedding, and thus they do not converge adapically. The three specimens are in contact with each other along the upper portion of their median region. These and the lack of any evidence of a sheet of budding stolons, suggest that this cluster was formed by preferential larval settlement. © Asociación Paleontológica Argentina.

Ritmitos de itu: Petrografia e considerações paleodeposicionais

Rhythmite samples from Varvite Quarry and Park of Itu (SP), and well cores from Rafard (SP), were analyzed by optical microscopy to describe the petrographic characteristics of their light-colored (siltstone) and dark-colored (shale) laminae. The light-colored ones are essentially siltstones, commonly with traces of sands; the mineralogical composition is mainly quartz, with traces of feldspar and scarce mica; quartz or calcite cementation occurs in variable intensity, the first one being more intense in the Itu rhythmites; parallel laminations, internally normal graded or massive are the most common sedimentary structures in these rocks; bioturbations and dropstones are commonly observed in Itu rhythmites; contacts between siltstone laminae, and siltstone beds and shale laminae are commonly non-erosive and sharp. Considering this study and the macroscopic description of these rhythmites it was possible to clarify many questions about their composition, as the grain-size of the light-colored beds composed by silt with subordinate dropped sand, and the inappropriate use of the term varvite to the most of the Itu rhythmites. It was also important to reinforce their proglacial lacustrine origin, with random freezing of lacustrine surface water, and only sporadic ice-contact.

Facies sedimentares e ambientes deposicionais da Formação Barbalha no vale do cariri, bacia do araripe, nordeste do Brasil

The Aptian Barbalha Formation represents the first unit of the post-rift sequence of the Araripe Basin and crops out at the slopes of the Aranpe plateau in the eastern part ot Aranpe Basin. The unit has also been named Kio da Batateira Formation, but this name is here used in its original definition as Batateira Beds, an interval of great lateral continuity and characterized by the presence of bituminous shales of the Alagoas Stage (P-270 palynological biozone). This paper presents the results of a stratigraphic analysis carried out along the outcrop belt in order to establish the facies architecture and to interpret deposicional environments of the siliciclastic Barbalha Formation. Detailed stratigraphic vertical sections were measured and correlated. They allowed the recognition of two depositional sequences characterized by fining upward arrangement of facies, beginning with fluvial deposits and ending with lacustrine deposits at their tops. The end of the first cycle is represented by black shales and brecciated limestones of the Batateira Beds that record a geologic event of regional magnitude and serve as meaningful long-distance stratigraphic mark. The second deposicional sequence overlies disconformably the Batateira Beds and begins with clast-supported conglomerates, which are covered by a succession of fluvial sandstones and minor intervals of pelitic rocks. The sandstone content diminishes towards the top and the upper part of the unit is characterized by the presence of ostracode-rich green shales. The Barbalha Formation is conformably overlaid by Late Aptian lacustrine limestones belonging to the Crato Member of the Santana Formation.

Immunohistochemical, tomographic and histological study on onlay iliac grafts remodeling

The information concerning the molecular events taking place in onlay bone grafts are still incipient. The objective of the present study is to correlate the effects of perforation of resident bone bed on (1) the timing of onlay autogenous graft revascularization; (2) the maintenance of volume/density of the graft (assessed through tomography); and (3) the occurrence of bone remodeling proteins (using immunohistochemistry technique) delivered in the graft. Thirty-six New Zealand White rabbits were subjected to iliac crest onlay bone grafting on both sides of the mandible. The bone bed was drill-perforated on one side aiming at accelerating revascularization, whereas on the other side it was kept intact. After grafts fixation and flaps suture all animals were submitted to tomography on both mandible sites. Six animals were sacrificed, respectively, at 3, 5, 7, 10, 20 and 60 days after surgery. A second tomography was taken just before sacrifice. Histological slides were prepared from each grafted site for both immunohistochemistry analysis [osteopontin, osteocalcin, type I collagen and vascular endothelial growth factor (VEGF) anti-bodies] and histometric analysis. The values on bone volume measured on tomography showed no statistic significance (P≥0.05) between perforated and intact sites. Grafts placed on perforated beds showed higher bone density values compared with non-perforated ones at 3 days (P≤0.05). This correlation was inverted at 60 days postoperatively. The findings from VEGF labeling revealed a tendency for earlier revascularization in the perforated group. The early revascularization of bone grafts accelerated the bone remodeling process (osteocalcin, type I collagen and osteopontin) that led to an increased bone deposition at 10 days. The extended osteoblast differentiation process at intermediate stages in the perforated group cooperated for a denser bone at 60 days. © 2008 Blackwell Munksgaard.

The first Triassic lungfish from South America (Santa Maria Formation, Paraná Basin) and its bearing on geological correlations within Pangaea

The Triassic fish faunas of the Southern Hemisphere are only known from a few sedimentary basins and the most productive sites are those from the Karoo Supergroup, in South Africa and the Sydney Basin of Australia. A single lungfish tooth plate ascribed to Ptychoceratodus cf. philippsi was recovered from Late Triassic (Carnian) red beds of southern Brazil and is described herein. This find extends to South America the palaeogeographic distribution of the genus, which occurs in the Early Triassic of Australia and South Africa and the Middle/Late Triassic of Europe and Late Triassic of Madagascar and India. The presence of this dipnoan solely in the uppermost part of the Santa Maria Formation suggests that the migration of Ptychoceratodus towards the Paraná Basin began not before the late Induan/early Olenekian (late Early Triassic). At that time, more humid (monsoonal) conditions prevailed in what is now southern Brazil, compared to semi-arid/desert conditions that dominated the Late Permian and possibly the earliest Early Triassic (the latter presumably not represented in the Paraná Basin). © The Geological Society of London 2008.

Reactores aeróbios de lecho fluidizado trifásico con circulación interna: Caracterización hidrodinámica y del soporte

Aerobic internal-loop reactors use active biomass attached in a supporting media (biofilm) with the advantage of retaining a big biomass concentration in a small physical space, removing carbonaceous matter and nitrogen in only one reactor. Liquid circulation occurs due to hydrostatic pressure difference produced by air injection in the riser. In biphasic conditions liquid circulation velocities, gas holdup and oxygen transfer coefficient in four different reactor configurations were studied. For the three-phase conditions, the same parameters in just one of those configurations were evaluated. Also, there were three granular supporting media characterized. On the other hand, the relationship between internal and external tube areas and supporting media concentrations influence the liquid velocity, gas holdup and oxygen mass transfer values and some important supporting media characteristics were observed and compared.