297 resultados para b-N-methylamino-L-alanine


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Dois eqüinos da raça Brasileira de Hipismo, machos, quatro anos de idade, apresentando sinais de poliúria-polidipsia, foram submetidos a completa avaliação clínica e a exames de sangue e urina. Não foram observadas alterações clínicas, hematológicas ou bioquímicas, a não ser um decréscimo na gravidade específica da urina de ambos os animais (1,009 e 1,008). Realizou-se um teste de privação de água de 24 horas e durante esse período a gravidade especifica da urina subiu gradualmente até atingir a normalidade (1,028 e 1,026, respectivamente). O comportamento anormal ocorreu por confinamento excessivo. Os resultados clínicos e de laboratório e o teste de privação de água indicam o diagnóstico de polidipsia psicogênica.

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Obejective To study the echocardiographic effects of isoflurane at an end-tidal concentration approximating 1.0 times the minimum alveolar concentration (MAC) in healthy unpremedicated dogs.Study design Prospective experimental trial.Animals Sixteen mature mongrel dogs of either sex weighing 11.06 +/- 2.72 kg.Methods After performing a baseline echocardiogram in the awake animal, anesthesia was induced with increasing inspired concentrations of isoflurane via a face mask until tracheal intubation was possible. Following intubation, the end-tidal concentration was decreased to 1.4% for the rest of the anesthetic period. Serial echocardiograms were recorded at 25, 40, and 55 minutes after the end-tidal concentration was reached.Results No changes were observed in heart rate. However, significant decreases were seen in left ventricular end-diastolic diameter (Mean maximal change: 13.8%), interventricular septal thickness during systole (15.2%), interventricular septal thickening fraction (72.2%), left ventricular free wall thickening fraction (63.5%), ejection fraction (39.9%), and fractional shortening (46.7%). In addition, peak flow velocities across mitral, pulmonic, and aortic valves were significantly lower than baseline values. Decreases were also observed in end-diastolic left ventricular volume index (approximately 32.1% from the awake value), stroke index (58.2%), and cardiac index (55.3%) when compared with awake measurements.Conclusions Our results indicate that 1 x MAC isoflurane caused significant myocardial depression in healthy dogs. These changes in myocardial function need to be considered carefully when isoflurane is to be used in dogs with poor cardiovascular reserve.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The citrus mealybug, Planococcus citri is an important pest attacking several crops. Biotic and abiotic factors, as well as the substrate they feed on influence its population. The present study was aimed to study the effect of some feeding substrates on mealybug development in laboratory conditions. Leaf disks of guava, fig and cherimoya were placed individually on an agar film inside a Petri dish. One egg of the insect was confined inside each container, replicated 60 times for each treatment. Containers were kept in a climatized chamber at 25[degree] C, 70 [plus or minus] 10% RH and 12 hours photophase. Insects reared on guava leaves showed a longer nymphal development, while the female longevity was longer on fig leaves. However, no statistical differences were found in the survival index, which showed values higher than 83%, suggesting that all substrates studied here were adequate for the development of P. citri.

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This work was carried out to study the development of populations of the genus Planococcus in different plant species, aiming at the verification of host specificity. The mealybugs Planococcus minor were obtained from cocoa plants (Theobroma cacao L.) and coffee crop (Coffea canephora L.), and Planococcus citri from citrus seedlings (Citrus sinensis L. Osbeck). Single eggs were transferred to Petri dishes containing leaf discs of cocoa (T. cacao cultivar Catongo), coffee (Coffea arabica L. cultivar Mundo Novo) and citrus (C. sinensis cultivar Pera Rio). They were maintained on a water-agar slide at 1%, with the abaxial surface turned upwards. The dishes were sealed with a PVC plastic film and kept in climatized chambers regulated at 25 [plus or minus] 1[degrees] C, 70 [plus or minus] 10% RH and 12 hours of photophase. For the mealybugs coming from cocoa plants, the citrus substrate prolonged the nymphal development of the males (26.0 days). In the nymphal period of females and males coming from coffee plants, the coffee substrate allowed a shorter duration of that period (19.2 and 21.3 days, respectively) and, in addition to having providing a longer longevity to the females (59.1 days). The highest rates of mortality were obtained when they were reared on the cocoa substrate, regardless of the host plant from which they were originally collected, and when kept on citrus, for insects collected on coffee plants. P. citri and P. minor have higher preference for coffee substrate regardless of the original host, thus showing partial host specificity.

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Realizou-se a contagem dos ovos não eclodidos, dos filhotes vivos e mortos de Podocnemis expansa oriundos de 327 ninhos naturais, localizados nas praias da Área de Proteção Ambiental (APA) - Meandros do Rio Araguaia, onde se determinou a porcentagem de eclosão dos ovos (94,63%); não eclosão (5,37%); sobrevivência (94,24%) e mortalidade dos filhotes (5,76%), e a média de filhotes mortos durante os 15 dias no berçário (0,97%). A média do total de filhotes por ninho foi determinada pela soma do número de filhotes vivos e mortos divididos pelo total de ninhos, enquanto que a média do total de ovos por ninho foi determinada pela soma do número de filhotes vivos, mortos e ovos não eclodidos divididos pelo total de ninhos. Com isso, obtiveram-se os valores médios do número de filhotes vivos (88,98 ± 23,94); mortos (0,37 ± 0,93); ovos não eclodidos (5,07 ± 9,57), e total de ovos (94,42 ± 21,30). A eficiência reprodutiva da população selvagem de P. expansa pode ser afetada por muitos fatores ambientais, como temperatura, umidade e precipitação. Além disso, fatores influenciados pelo homem, como a presença de produtos químicos na água e a possibilidade de doenças infecciosas, também têm impacto significativo. Os dados dos índices reprodutivos obtidos neste estudo são indispensáveis para futuras investigações de anomalias de incubação.

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Adequate environmental temperature during the brooding period is very important to future broiler performance. Thus, the objective of this study was to investigate the extent to which environmental temperature affects the body weight and cloacal and surface (back, head, wing, and shank) temperatures. The study also investigated the sensible heat loss by radiation of broiler chicks reared at three environmental temperatures (35, 25, and 20 degrees C) up to 7 days of life. The results showed that chicks raised at low environmental temperature (20 degrees C) had lower body weight at 7 days of age. Birds kept at 20 degrees C also had significantly lower cloacal and surface temperatures than did other birds. The most marked difference was seen in the shanks. These findings revealed that body weight declined in chicks reared at 20 degrees C, and radiant heat loss (W) was nine times higher than for the birds kept at 35 degrees C at 7 days of age.

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Somatotrophic and thyroid hormones were determined around the onset of reproduction in broiler breeders reared in two different housing systems [dark, close-sided house (CH) and conventional, open-sided house (OH)]. In both groups age-related changes were obvious for thyroxine (T-4), growth hormone (GH) and insulin-like growth factor (IGF-1); levels of T-4 decreased, especially between 24 and 28 weeks in both groups; concomitantly GH sharply increased over the same period. A transient peak in triiodothyronine (T-3) occurred between 25 and 27 weeks. The effect of housing was only present after the onset of lay. Between weeks 27-28 and the end of the period studied, the CH group showed higher levels of GH and T-3 but lower T-4 levels as compared to the OH group. A significant increase in GH after onset of lay, without any significant rise in T-3 or in IGF-I, could point to a relative insensitivity to high plasma GH levels. Changes at GH receptor level, together with an increased pituitary GH secretion and/or decreased GH turnover may be expected. This may indicate that hypothalamo-pituitary changes at the onset of lay not only imply changes of gonadotrophic cell function, but also other hormonal axes. The relatively decrease in T-4 without changes in T-3, may point to a decrease in the activity of the thyrotropic axis.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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The sexual precocity and fertility of bovines have great impact on the economic success of commercial cattle herds, where some reproductive traits have been adopted as selection criteria. However, the majority of these traits depend on reproductive events of the females and, with exception of the scrotal circumference, few studies approach others andrological traits. The estimation of heritabilities and genetic correlations of testicular and seminal traits and also sexual behaviour, will allow to provide alternatives for the design of more appropriate selection strategies for fertility, together with other economic traits.