Development and validation of a stability-indicative turbidimetric assay to determine the potency of doxycycline hyclate in tablets

The doxycycline (DOX) is a broad-spectrum antibiotic used in several countries. This drug is part of the list of medicines to the SUS (Unified Health System), a model of health care in Brazil. This study describes the development and validation of a microbiological assay, applying the turbidimetric method for the determination of DOX, as well as the evaluation of the ability of the method in determining the stability of DOX in tablets against acidic and basic hydrolysis, photolytic and oxidative degradations, using Escherichia coli ATCC 10536 as micro-organism test and 3×3 parallel line assay design, with nine tubes for each assay, as recommended by the Brazilian Pharmacopoeia. The developed and validated method showed excellent results of linearity, selectivity, precision, accuracy and robustness. The assay is based on the inhibitory effect of DOX using Escherichia coli ATCC 10536. The results of the assay were treated by analysis of variance and were found to be linear (r= 0.9986) in the range from 4.0 to 9.0μg/mL, precise (repeatability R.S.D.= 0.99 and intermediate precision was confirmed by statistical analysis the mean values obtained from analysis by different analysts) and exact (97.73%). DOX solution exposed to direct UV light, alkaline and acid hydrolysis and hydrogen peroxide causing oxidation were used to evaluate the specificity of the bioassay. Comparison of bioassay and liquid chromatography showed differences in results between methodologies. The results showed that the bioassay is valid, simple and useful alternative methodology for DOX determination in routine quality control.

Doxycycline hyclate: a review of properties, applications and analytical methods

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Honorários praticados por operadoras de planos odontológicos e pelo SUS em relação aos definidos pelo Conselho Federal de Odontologia

Aim: The present study investigated the fees paid by dental insurance companies to dental surgeons, referrent to groups of dental procedures. Materials and Methods: The fees offered by dental insurance companies were defined according to the average values from the price tables of 5 insurance plans that offered national coverage. These average values and the values paid by the Brazilian Unified Health System (SUS) were compared with those set forth in the VRCC table from Brazilian Federal Dental Council (CFO). For comparison, average values were obtained for the following groups of procedures: preventive, aesthetic, endodontic, and surgical. The values of these preventive procedures were compared with other groups of procedures. Results: The plans applied an average discount of approximately 54.51% upon the prices suggested in the CFO price list. The highest discount was observed within the group of preventive procedures (57.09%, on average). Conclusion: The evaluated health insurance companies in vestigated in the present study are applying an abusive discount upon the prices suggested in the CFO price list. It's necessary to identify alternatives to reverse this situation.

Avaliação da sensibilidade à insulina e concentração do cortisol salivar em gestantes com doença periodontal

Pós-graduação em Odontologia Preventiva e Social - FOA

Avaliação e resolutividade do serviço odontológico no Sistema Único de Saúde

Pós-graduação em Odontologia Preventiva e Social - FOA

Aspectos sociodemográficos e epidemiológicos dos casos de hanseníase em uma área endêmica: conhecimentos e experiências de cirurgiões-dentistas

Pós-graduação em Odontologia Preventiva e Social - FOA

Acesso às informações de saúde e inclusão digital: a percepção dos Conselheiros Municipais de Saúde de Botucatu - SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Vivências anímicas e percepção subjetiva de qualidade de vida: perspectiva de servidores públicos nos âmbitos da saúde e da educação

This study was qualitative in nature and aimed to investigate the possible interference of spiritual experiences with the subjective perception of quality of life. The perspectives of civil servants in education and health services were compared. The study was developed through bibliographic and exploratory research, and the instrument used for data collection for the exploratory research was the WHOQOL-bref, which was applied in two stages: before and after experiences with spiritual activities. The purposive sample used in this study was composed of civil servants working in health and education administrative sectors of the Brazilian Unified Health System and a State University, respectively, with the intention of broadening the understanding about the perception of quality of life in civil servants from different sectors. The exploratory research of the study was carried out in three stages. The first was a two-phase data collection, before and after experiences with spiritual activities offered to civil servants who worked in the health care field, as well as the analysis of the results of this step. The second stage was the collection of data before and after experiences with spiritual activities offered to civil servants who worked in the administrative sector of education in a public university, besides the analysis of the results of this step. The third stage consisted of a comparison of the results obtained in the preceding two steps. The data resulting from the use of the research instrument for both samples were analyzed via Thematic Content Analysis. A questionnaire containing 26 questions based on four main themes was used. The first two questions were related to perceived quality of life and health satisfaction and they were not part of the specific domains. The other 24 questions were divided between the four themes: physical domain, psychological domain, domain of social relations, and environmental domain. The results...

O princípio da integralidade no Sistema Único de Saúde (SUS): utopia?

The Brazilian Federal Constitution promulgated in 1988 created the concept of Social Welfare, which is based on the triad: Health, Social Security and Social Assistance. The Unified Health System (SUS) was then instituted. SUS is a conquest of a society that seeks social justice, integrality, equalitarian and universal access to health services. In the present essay, I succinctly discourse on the various meanings of integrality. I present the theoretical basis of complexity and transdisciplinarity by opposing to reductionism, aiming at showing that, by means of transdisciplinarity and intersectoriality, integrality can be achievable.

Acolhimento na percepção dos enfermeiros da atenção primária à Saúde

Objective: This paper is intended to explore how nurses working in primary health care perceive acceptance. Method: It is based on a qualitative study conducted through interviews with 21 nurses from eight municipalities in the State of São Paulo. A phenomenological approach was used in data analysis. Results: It was found that nurses' understanding of acceptance, at certain points in time, comes close to the principles and guidelines of the Unified Health System (SUS). Conclusions: Nursing professionals manifest difficulties with the structure, organization and management of health services and express actions and beliefs related to the traditional model of care. The various stakeholders need to join forces if acceptance is to be achieved.

Fatores de risco relacionados à perda de produtividade laboral e aumento dos custos ambulatoriais de pacientes atendidos por unidades básicas de saúde

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de biomassa e teor de cumarina em folhas de guaco (Mikania laevigata Sch. Bip. ex Baker) em diferentes idades da planta

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo da diversidade genética e química de Uncaria tomentosa Willd. ex Roem. & Schult. e Uncaria guianensis Gmell. de populações naturais localizadas na Amazônia

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Acessibilidade e resolutividade em assistência oftalmológica para o SUS

Pós-graduação em Bases Gerais da Cirurgia - FMB

Práticas integrativas e complementares no SUS: revisão integrativa

Pós-graduação em Saúde Coletiva - FMB