Validation of a reference control for an SYBR-Green fluorescence assay-based real-time PCR for detection of bovine herpesvirus 5 in experimentally exposed bovine embryos

The objective of this study was to optimize an internal control to improve SYBR-Green-based qPCR to amplify/detect the BoHV-5 US9 gene in bovine embryos produced invitro and experimentally exposed to the virus. We designed an SYBR-Green-based binding assay that is quick to perform, reliable, easily optimized and compares well with the published assay. Herein we demonstrated its general applicability to detect BoHV-5 US9 gene in bovine embryos produced invitro experimentally exposed to BoHV-5. In order to validate the assay, three different reference genes were tested; and the histone 2a gene was shown to be the most adequate for normalizing the qPCR reaction, by considering melting and standard curves ( p<0.05). On the other hand, no differences were found in the development of bovine embryos invitro whether they were exposed to BoHV-5 reference and field strains comparing to unexposed embryos. The developed qPCR assay may have important field applications as it provides an accurate BoHV-5 US9 gene detection using a proven reference gene and is considerably less expensive than the TaqMan qPCR currently employed in sanitary programs. © 2013 Elsevier Ltd.

Modelagem de distorções entre realizações de referenciais geodésicos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

PPP em tempo real com estimativa das correções dos relógios dos satélites no contexto de rede GNSS

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Métodos de estimativa da evapotranspiração de referência para a mesoregião do Triângulo Mineiro e Alto Paranaíba-MG

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do nitrogênio nas propriedades anelásticas de Nb e ligas Nb-1,0%pZr

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização climática e comparação de métodos de estimativa de evapotranspiração de referência para regiões do Estado do Ceará

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise de deformação da rede GNSS/SP com base na teoria da elasticidade

Pós-graduação em Ciências Cartográficas - FCT

Influence of distance, geometry and number of control stations on quality of local geodetic networks for the purpose of georeferencing of rural properties

The implementation of local geodetic networks for georeferencing of rural properties has become a requirement after publication of the Georeferencing Technical Standard by INCRA. According to this standard, the maximum distance of baselines to GNSS L1 receivers is of 20 km. Besides the length of the baseline, the geometry and the number of geodetic control stations are other factors to be considered in the implementation of geodetic networks. Thus, this research aimed to examine the influence of baseline lengths higher than the regulated limit of 20 km, the geometry and the number of control stations on quality of local geodetic networks for georeferencing, and also to demonstrate the importance of using specific tests to evaluate the solution of ambiguities and on the quality of the adjustment. The results indicated that the increasing number of control stations has improved the quality of the network, the geometry has not influenced on the quality and the baseline length has influenced on the quality; however, lengths higher than 20 km has not interrupted the implementation, with GPS L1 receiver, of the local geodetic network for the purpose of georeferencing. Also, the use of different statistical tests, both for the evaluation of the resolution of ambiguities and for the adjustment, have enabled greater clearness in analyzing the results, which allow that unsuitable observations may be eliminated.

Cephalometric analysis for the diagnosis of sleep apnea: a comparative study between reference values and measurements obtained for Brazilian subjects

OBJECTIVE: To verify if the reference values of Sleep Apnea cephalometric analysis of North American individuals are similar to the ones of Brazilian individuals presenting no craniofacial anomalies. The study also aimed to identify craniofacial alterations in Obstructive Sleep Apnea-Hypopnea Syndrome (OSAHS) patients in relation to individuals without clinical characteristics of the disease through this cephalometric analysis. METHOD: It were used 55 lateral cephalograms consisting of 29 for the control group of adult individuals without clinical characteristics of OSAHS and 26 apneic adults. All radiographs were submitted to Sleep Apnea cephalometric analysis through Radiocef Studio 2.0. The standard values of this analysis were compared, by means of z test, to the ones obtained from the control group and these were compared to values from apneic group through Student's t test. RESULTS: There were no significant differences between values obtained from control group and standard values. On the group of OSAHS patients it was observed a decrease on the dimensions of upper airways and an increase on the soft palate length. CONCLUSIONS: The standard values of Sleep Apnea analysis can be used as reference in Brazilian individuals. Besides, through lateral cephalograms it was possible to identify craniofacial alterations in OSAHS patients.

Proposta de modelo de processo de produção de conteúdo em emissora de TV digital universitária

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Reference Gene Selection for Gene Expression Analysis of Oocytes Collected from Dairy Cattle and Buffaloes during Winter and Summer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Integrated assessment of metal contamination in sediments from two tropical estuaries

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A Reference Architecture based on Reflection for Self-adaptive Software

Self-adaptive Software (SaS) presents specific characteristics compared to traditional ones, as it makes possible adaptations to be incorporated at runtime. These adaptations, when manually performed, normally become an onerous, error-prone activity. In this scenario, automated approaches have been proposed to support such adaptations; however, the development of SaS is not a trivial task. In parallel, reference architectures are reusable artifacts that aggregate the knowledge of architectures of software systems in specific domains. They have facilitated the development, standardization, and evolution of systems of those domains. In spite of their relevance, in the SaS domain, reference architectures that could support a more systematic development of SaS are not found yet. Considering this context, the main contribution of this paper is to present a reference architecture based on reflection for SaS, named RA4SaS (Reference Architecture for SaS). Its main purpose is to support the development of SaS that presents adaptations at runtime. To show the viability of this reference architecture, a case study is presented. As result, it has been observed that RA4SaS has presented good perspective to efficiently contribute to the area of SaS.

Transformação de coordenadas com modelagem de distorções entre SAD69 e SIRGAS2000 com o uso de Thin-Plate Splines

Several countries have been passed by change processes in their fundamental geodesic structure with the focus on the adoption of geocentric reference systems. In Brazil, the adoption of the SIRGAS2000 evolves the coexistence of two realizations from the COrrego Alegre system, two realizations from the SAD69 system and one realization from the SIRGAS2000 system. To make use of products in the old reference systems, methods of coordinate transformation between the existent reference frames are necessary. So, in this paper one solution for the transformation between coordinates from different reference frames, based on Thin-Plate Splines (TPS), that allows the estimation of parameters from one linear transformation and also one non-linear model is presented. The TPS model was developed to work with tridimensional coordinates and in this paper the results and analysis are performed with simulated data and also with data from the official Brazilian Geodetic System (SGB). In the check points from SAD69 stations (realization of 1996 - SAD69/96), the values of RMSE obtained were of 78,2 mm in latitude and 67,5 mm in longitude, before the transformation to the SIRGAS2000. In the comparison between the TPS model and ProGriD (Brazilian software provided by IBGE), the statistical indicators were reduced in 97%, by using the TPS model. Based in the obtained results from real dataset, the TPS model appears to be promising, since it allows improving the quality of transformation process with simultaneous distortion modeling.

Evaluation of the Tropical Rainfall Measuring Mission 3B43 product over an inland area in Brazil and the effects of satellite boost on rainfall estimates

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)