Relações entre condutividade hidráulica, transmissividade, condutância longitudinal e sólidos totais dissolvidos para o aquífero livre de Caçapava do Sul (RS), Brasil

A água subterrânea é um importante recurso ao abastecimento público, principalmente para comunidades periféricas em grandes cidades ou pequenos municípios, onde a instalação ou prolongamento de sistemas de distribuição são economicamente inviáveis. Estudos voltados à análise da vulnerabilidade de aquíferos são relevantes para subsídio a políticas públicas em saneamento básico e preservação ambiental. Este estudo contou com o cadastro de 23 poços rasos localizados no município de Caçapava do Sul (RS), onde foram realizadas medidas de resistividade elétrica e cargabilidade por meio de geofísica, e medidas diretas de condutividade hidráulica e sólidos totais dissolvidos. A análise estatística dos dados demonstrou diversos graus de correlação entre parâmetros, basicamente condicionados a fatores geológicos como porosidade, permeabilidade e sólidos totais dissolvidos.

Efeitos da adubação orgânica e da época de colheita na qualidade da matéria-prima e nos rendimentos agrícola e de açúcar mascavo artesanal de duas cultivares de cana-de-açúcar (cana-planta)

Conduziu-se este trabalho com o objetivo de estudar os efeitos de três sistemas de adubação (30 t. ha-1 de esterco de curral, 3,5 t.ha-1 de esterco de galinha e adubação química - 120 kg.ha-1 de P2O5 e de K2O no plantio + 60 kg.ha-1 de N em cobertura) e três épocas de colheita da cana (julho, agosto e setembro de 2003), na qualidade da matéria-prima e nos rendimentos de colmos e de açúcar mascavo de duas cultivares de cana-de-açúcar (SP79-1011 e RB72454). O experimento foi instalado em área do Alambique JM, Perdões, MG. O delineamento experimental foi o de blocos casualizados, em esquema fatorial (2 x 3 x 3), com três repetições. Não houve efeito dos fertilizantes nos rendimentos de colmos e de açúcar mascavo das cultivares estudadas. Verificou-se efeito de épocas de colheita no rendimento de colmos, com destaque para os meses de agosto e setembro. No entanto, para rendimento de açúcar mascavo nenhuma diferença foi observada. Assim, nas condições deste trabalho, é viável a substituição da adubação química pela orgânica (esterco de curral ou de galinha), sem perdas na qualidade da matéria-prima e nos rendimentos de colmos e de açúcar mascavo artesanal, sendo que os meses de agosto e setembro foram os que proporcionaram matéria-prima de melhor qualidade e maiores rendimentos de colmos.

Análise de crescimento e anatomia foliar da planta medicinal Ageratum conyzoides L. (Asteraceae) cultivada em diferentes substratos

Ageratum conyzoides L. (Asteraceae) é uma espécie herbácea que ocorre em áreas de cerrado e, por se tratar de planta medicinal, é cultivada em escala familiar. O objetivo deste trabalho foi estudar possíveis alterações no desenvolvimento das plantas quando cultivadas em solos com texturas diferentes (solo de cerrado, franco arenoso ou solo de terra roxa, muito argiloso). Foram analisados aos 40, 70 e 90 dias, o comprimento do caule e da raiz, massa seca da parte aérea (caule e folhas) e das raízes, número de folhas e área foliar; além de parâmetros anatômicos foliares, como a densidade de tricomas. Os resultados mostraram que os comprimentos do caule e da raiz aumentaram no decorrer do experimento, independentemente do substrato (com exceção do comprimento da raiz de plantas cultivadas em solo de terra roxa). Porém, o comprimento do caule foi sempre maior nas plantas cultivadas em solo de cerrado, enquanto o comprimento da raiz foi significativamente maior somente aos 90 dias de cultivo neste solo. As biomassas secas da raiz e do caule também foram maiores nas plantas cultivadas em solo de cerrado por 70 e 90 dias, respectivamente. A massa seca foliar foi maior nas plantas cultivadas em solo de cerrado a partir dos 70 dias de experimento. Esse aumento foi devido ao maior número de folhas produzidas e a maior área foliar dessas plantas. No geral, a anatomia foliar não diferiu, porém o número de tricomas glandulares foi maior nas plantas cultivadas em solo de cerrado. Os resultados indicaram que esta espécie tem melhor desenvolvimento quando cultivada em solo mais arenoso.

Sistemas de Alimentação com Livre Escolha e Semi Livre Escolha para Poedeiras

This research was carried out to verify the ability of laying hens to select food in order to meet their requirements for protein and energy. Ninety-six Hy-Line White laying hens, 45 weeks old, were allotted to a randomized block design with two blocks (two ranges of body weight), four treatments, and three replicates of four hens in each block. The treatments consisted of four different feeding systems: I-Conventional feeding represented by a complete ration composed of 60% ground corn and 40% protein concentrate; 2-Free-choice feeding with ground corn, protein concentrate and oyster shells fed in feeders with three separate compartments; 3-Semi free-choice feeding with whole corn grain, protein concentrate and oyster shells in the same proportion in one feeder; 4-Free-choice feeding with whole corn grain, protein concentrate and oyster shells fed in feeders with three separate compartments. The results indicated that the hens, in spite of age, adapt to different feeding systems, and that they can select feed to meet their nutrient requirements. The semi free-choice and free-choice feeding systems with whole corn grain resulted in the same performance compared to conventional feeding, but shell quality was not improved by oyster shell supplementation. However, the worst performance was with free-choice feeding with ground corn, which indicated that in the free-choice feeding system the use of whole corn grain is recommended.

Expression of pathogenicity-related genes of Xylella fastidiosa in vitro and in planta

Xylella fastidiosa is responsible for several economically important plant diseases. It is currently assumed that the symptoms are caused by vascular occlusion due to biofilm formation. Microarray technology was previously used to examine the global gene expression profile of X. filstidiosa freshly isolated from symptomatic plants or after several passages by axenic culture medium, and different pathogenicity profiles have been obtained. In the present study the expression of some pathogenicity-related genes was evaluated in vitro and in planta by RT-PCR. The results suggest that adhesion is important at the beginning of biofilm formation, while the genes related to adaptation are essential for the organism's maintenance in planta. Similar results were observed in vitro mainly for the adhesion genes. The pattern of expression observed suggests that adhesion modulates biofilm formation whereas the expression of some adaptation genes may be related to the environment in which the organism is living.

Doses de zinco em diferentes condições de acidez de um solo de cerrado. II. Nível crítico na planta

In this work it was demanded to verify the zinc critical levels in soybean plants cultivated in a Dark-Red Latosol. So the experiment was carried out in vases with 4 liters of soil, which has received five doses of Zn (0, 1.0, 2.0, 3.0 and 4.0 ppm) in three correction levels. It was verified that the critical levels of the nutrient in plant (for 90% from highest production) was between 36 and 53 ppm to the dry matter yield and grains in treatments with correction.

Estádio de Desenvolvimento do Milho. 1. Efeito sobre a Produção, Composição da Planta e Qualidade da Silagem

The objective of this study was to evaluate the influence of maturity on the production, stalk/leaf/ear relationship, chemical composition, and quality of corn silage (Zen mays, L.). Four harvests were made from the early milk (23.49% DM) to the semi-hard dough stage. (30.70%DM). After these evaluations, the corn was ensiled in 200 liter metal drums. After six months of storage, the quality of the silages was determined. The corn dry matter production was higher at the dough and semi-hard dough stages (9866 and 9896 t/ha, respectively) than in the early milk stage (7460 t/ha). The ears with the maturity showed a increase in dry matter (20.93 to 43.51%), acid detergent fiber decrease (32.46 to 18.62%), and maintainance of the crude protein content (average = 8.27%). The stalks and leaves decreased from 33.31 to 27.18% and from 34.17 to 23.66%, respectively, while the ears increased from 32.52 to 49.16% from the early milk to the semi-hard dough stages. All experimental silages attained good quality, independent of corn development stages. The corn can be ensiled from the early milk and soft dough stages without a decrease in silage quality, but the restricting factor under those conditions was a lower dry matter content (24.35 and 28.65%, respectively).

Atividade da enzima redutase do nitrato e teores de prolina livre em raízes de cana-de-açúcar sob os estresses hídrico e ácido no solo

The aim of this work was evaluate the physiological indicators of association between water deficiency and soil acidity, by determining the nitrate reductase activity, the levels of free proline and initial growth of the root system of seedlings of sugarcane cv. IAC91-5155. After 30 days, the seedlings were transferred to plastic pots with 12 dm3 of dystrophic alic Red Latosol (Oxisol) and submitted to association of three treatments of water availability: no stress (70%), moderate stress (55%) and severe stress (40%), in according with field capacity and three acidity treatments: no stress (55%), moderate stress (33%) and severe stress (23%), considering the base saturation. The experimental design was that of random blocks under factorial scheme of 3x3, with four replicates. After 60 days under the stress association, the levels of free proline, the nitrate reductase activity and the growth of the sugarcane roots system were evaluated in seedlings of sugarcane. The nitrate reductase enzyme activity can be considered a physiological indicator of the effect of the association of acid and water stress in moderate conditions in soil, while the free proline can be considered physiological indicator to both stress in severe conditions. Water deficiency increasing reduced growth of sugarcane roots.

Brosimum gaudichaudii Trecul (Moraceae): Da planta ao medicamento

The chemical, biological, pharmacological and toxicological study of plants used in medical treatments by different societies around the world has been intensifying over the years, primarily in the search for new herbal, homeopathic or even allopathic medicines. Motivated by this interest, we are investigating some Brazilian plant species, among them a tree, Brosimum gaudichaudii Trecul (Moraceae), found in Brazil's savannah-like prairies and scrub-forest (cerrado) and mesophytic forest. Several extracts have been made and separated into fractions and some compounds have been isolated - principally coumarins - and their respective structures analyzed by spectrophotometric and other methods. By collaborating with researchers in other fields, we have been able to carry out various bioassays and even basic clinical tests, namely: determination of anti-helminthic, anti-microbial, photosensitizing, anticancer and mutagenic activities, toxicological and pathogenic studies, with the aim of discovering a genuinely Brazilian herbal or homeopathic medicine.

Comportamento cinético do cloro livre em meio aquoso e formação de subprodutos da desinfecção

One indirect approach to predict the disinfection by-product (DBP) formation potential for a given water source is by evaluation of the kinetic behavior of free chlorine in the liquid phase and chlorine demand determination for different operation conditions of the chlorination process. The objective of this work was to evaluate the kinetic behavior of free chlorine in water or a number of different raw water sources, as well as to investigate the impact of the coagulation process on chlorine demand reduction and DBP formation. It was observed that the higher the total organic carbon (TOC) removal efficiency through coagulation, the lower the liquid phase chlorine demand. Regarding trihalomethane (THM) formation, a ratio of 28 ug/L formed per mg/L of applied chlorine was observed for the waters employed in the experimental investigation.

Helmintos parasitos de mocós (Kerodon rupestris Rodentia: Caviidae) de vida livre e de cativeiro, no semi-árido nordestino Brasileiro

This study shows the helminthic fauna of 16 rock cavies (Kerodon rupestris) from the semi-arid region in the State of Rio Grande do Norte, Brazil. Nine animals were captured from the wild and seven from a captive population located at the Centro de Multiplicação de Animais Silvestres, city of Mossoró, were examined in this study. The animals were slaughtered, and post mortem analysis of the digestive tract, trachea, heart, lungs, liver, pancreas, and kidneys were done in search for the presence of parasites. Five species of nematode (Paraspidodera uncinata, Trichostrongylus colubriformis, Trichuris gracilis, Trichuris muris, and Vianella lenti) and one species of cestode (Thysanotaenia congolensis) were identified.

Germinación de semillas después del fuego: Un estudio con una planta que habita áreas no propensas al fuego

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sistema de reprodução, parentesco e tamanho efetivo em sementes de polinização livre de populações fragmentadas de Copaifera langsdorffii Desf. por análise de locos microssatélites

Anthropogenic changes in the reproductive population density can affect the mating system and result in an increase in selfing and correlated matings. This study investigated the mating system in small fragmented populations of the insect pollinated tropical tree species Copaifera langsdorffii, using microsatellite loci and the mixed and correlated mating models. Open-pollinated seeds were collected from 15 seed-trees located in a small forest fragment (4.8 ha), denominated Bosque and from 14 other seed-trees located in other small forest fragments of the north-western region of São Paulo State. No significant differences were observed between the seed-trees from Bosque (tm=0.933±0.028) and other fragments (tm=0.971±0.032), although these estimates were significantly different from 1.0, suggesting that selfing was occurring. Differences between multilocus and unilocus outcrossing rate were significantly high in both seed-trees of Bosque (tm -ts=0.478±0.05) and other forest fragments (tm -ts=0.475±0.018), suggesting a spatial genetic structure in those stands. The results also showed high rates of correlated mating in the samples, indicating that a good part of the offspring were full-sibs. As a consequence of selfing, mating among relatives and correlated matings, the coancestry within families was equally high in the seed-trees of Bosque ((Θ=0.237) and in the seed-trees of the other forest fragments (Θ=0.241) and the effective population size was lower than expected in panmitic populations (Ne<4). The results were discussed, focusing on the sample size of seed-trees to collect seeds for genetic conservation and enviromental reforestation.

Variação e parâmetros genéticos em teste de progênies de polinização livre de Peltophorum dubium (Sprengel) taubert em Luiz Antonio -SP

Intensive deforestation and forest fragmentation of the Brazilian forest biomes has contributed to increase the number of trees species under the risk of extinction. Peltophorum dubium (Sprengel) Taubert is one of these species. Some of its populations are being conserved ex situ by the Instituto Florestal de São Paulo through provenance and progeny tests. The aim of this study was to investigate the genetic variation and to estimate genetic parameters at age 24 years in a progeny test of P. dubium established in Luiz Antônio, São Paulo State, Brazil. The trial was established in a random block design, with six replications, five plants per plot and 18 open-pollinated progenies. Measured were: diameter at breast height (DBH), height and stem form. The genetic parameters heritability, genetic variation and effective population size were estimated. Significant genetic differences were observed only for DBH. This trait also presented a high coefficient of genetic variation (CV g=4.8%) and heritability, especially among progeny means (h m 2=0.6607). This indicates that DBH is the most indicated trait for selection in the population. The effective population size conserved ex situ in the test was estimated to be 38.9. Concerning genetic conservation, although the effective population size in the test is small, the values of the genetic variation and of the heritability indicate that the ex situ population has sufficient genetic variation and potential to respond to changes promoted by natural and artificial selection.

Correlação e análise de trilha de produtividade de grãos e seus componentes e caracteres de planta em cártamo (Carthamus tinctorius L.) e mamona (Ricinus communis L.)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)