Indução de morte celular por apoptose decorrente da irradiação com Raios-X: um ensaio com células mamárias humanas tumorais

Apoptosis is a form of programmed cell death selectively removes abnormal cells, and thus contributes to maintaining the balance of the dynamics of cell reproduction. Therefore the verification of the occurrence of apoptotic cell death after a pathological stimulus is crucial for the analysis of the maintenance of normal cell cycle of a given tissue or organ. In this experiment were used cells lines human mammary tumor MDAMB231, T47, MCF7, which were irradiated with X-rays at a dose of 5 Gy in a time interval of 15 seconds, and filtration of 1mm aluminum. Samples containing the cells were grown in a specific culture medium, containing fetal bovine serum and growth factor, and two samples were prepared with each of the cell lines, one to be irradiated, and another that has not been irradiated, which denoted by negative control of the irradiation. The primary goal of the experiment was to verify and compare the rates of apoptosis in each cell lines, in which were irradiated and that were not irradiated, using flow cytometry as a method for detecting apoptotic cell death in together with specific markers annexin V and propidium iodide. Data from the readings made by flow cytometry were analyzed and interpreted using the software WinMDI statistical graph. By comparing the indices relating to the readings of positive and negative for specific markers of apoptosis, based on differences in the statistical data presented lectures regarding the cellular irradiated and not irradiated, collude cells in question once... (Complete abstract click electronic access below)

Proliferação e Morte Celular (Apoptose) avaliadas em ratos Lewis após tratamento com mistura de praguicidas em baixas doses

The use of pesticides in Brazil has intensified over the years. And since 2009 it was ranked as the largest market for pesticides worldwide. The consequential diffuse contamination of the environment is therefore due to the increasing number of chemicals of different classes, origins and modes of action. Little is known about the action of pesticides on human health in situations of co-exposure. Possible toxic effects are not restricted to agricultural and industrial workers, but also the general population that may be exposed continuously to its residues in food and water. Although these pesticides are mostly present in the environment at low doses, it must be considered that possible cumulative or synergistic effects may occur when there are concurrent or sustained exposure for two or more of these agents, which can lead to late manifestation of subclinical damages, sometimes irreversible. Thus, the specific objective of this study was to assess the effect of carcinogenesis promotion of a mixture of pesticides at low doses and analyze the phenomena of cell proliferation and apoptosis in rat liver. A total of 50 male Lewis rats was separated into 5 groups for 8 weeks in a medium term hepatocarcinogenesis model. The three different classes of pesticides (dieldrin, dicofol, endosulfan, dichlorvos and permethrin), whose residues were detected by ANVISA during the period from 2001 to 2005 in tomatoes cultures, were added to the feed of rats initiated to hepatocarcinogenesis with diethylnitrosamine (DEN- 200mg/kg ip). We used two different mixtures, one with no toxic effects at doses (MEX1) referring to the NOEL (no-observed-effect level) and another at doses LOEL / LEL / LOAEL (Lowest-observed-effect level / Lowest-effect level / Lowest -observed-adverse-effect level), to the installation of adverse effects (MEX2), derived from chronic studies. All animals ...(Complete abstract click electronic access below)

Morte e morrer: opinião dos profissionais de saúde de uma unidade de terapia intensiva pediátrica

Presently, the dying process and death most often occur in hospitals and, particularly, in Intensive Care Units (ICU), where patients’ lives are prolonged thanks to advanced technological devices and highly efficient medicines. To learn about the opinion of health care professionals working at a Pediatric Intensive Care Unit in relation to the dying process and dying. This is a descriptive quantitative study. A questionnaire was applied to the unit’s staff members from June to August, 2011. Data were statistically analyzed. Twenty-five professionals answered the questionnaire, and 72% faced death as a natural life process. 60% felt compassion, but that feeling did not interfere with how they cared for patients. Concerning their professional training, 52% reported not to have received any concerning patients’ caregivers in the dying process or death; therefore, they experienced such situation when they were already working, and 76% reported to be interested in updating courses on that theme. Further discussion about this topic during academic education is necessary. It is also necessary to provide health care professionals with specialization courses, debates and experience exchange so that they can better understand and deal with their feelings and limitations in face of death and thus give better care to patients and relate to patients’ families during the dying process of a loved one

Estimação do risco de morte de recém-nascidos utilizando logica fuzzy tipo-2 intervalar

The main objective of the presented study is the development of a predictive interval type-2 fuzzy inference system in order to estimate the mortality risk for a newborn, to be used as an auxiliary tool for decision making in medical centers where there is a lack of professionals for this purpose and, afterwards, to compare its performance to a type-1 fuzzy system. The input variables were chosen due to their acquisition ‘simplicity, not involving any invasive tests, such as blood tests or other specific tests. The variables are easily obtained in the first few minutes of life: birth weight, gestational age at delivery, 5-minute Apgar score and previous report of stillbirth. Databases from the DATASUS were used to validate the model. 1351 records from the city of São José dos Campos, a mid-sized city in the São Paulo state’s countryside, were considered in this study. Finally, an analysis using the ROC curve was performed to estimate the model’s accuracy

Mediadores da via intrínseca da morte celular programada relacionados à infecção in vitro pelo herpesvirus bovino tipo 5

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Vida e morte em diálogo com a voz narrativa, o tempo e o espaço em Mrs. Dalloway, To The Lighthouse e Between the Acts de Virginia Woolf

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prostituição e morte em Maggie: a girl of the streets: uma leitura feminista sobre a Slum fiction norte-americana de Stephen Crane

Pós-graduação em Letras - FCLAS

Efeitos da dose única de parecoxibe sobre a morte celular em rins de ratos submetidos à hemorragia aguda

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mito e concretude: a morte na poesia de Angelus Silesius

This study intends to get a general overview on the German Angelus Silesius (1624- 1677)’s poetry about the way he discusses death. For the purpose of postulating some hypotheses about the meaning of death in the historical period in which the poet lived, we will comment some poems by identifying clues which reclaim the period’s moral.

Uma novela, um filme, o amor e a morte em Veneza

This article compares Thomas Mann´s The death in Venice with its cinematographic version directed by Luchino Visconti.

Uma representação irônica da morte e da escrita

The novel As intermitências da morte, by José Saramago (2005a), presents an imaginary narrative that treats one of the most classical themes of universal literature. Besides that, the novel discusses the question of fictional discourse construction, showing the potentialities (and limits) of language as an intermediation between life and art.

Morte na antilírica de João Cabral de Melo Neto

The critic João Alexandre Barbosa, points in the work Agrestes (1981-1985), of the Brazilian poet João Cabral de Melo Neto, the presence of individualized death. Thus way, this study proposes to analyse the meanings of lyrism and first-person enunciations, in the work Agrestes, a trace gradually eliminated from Cabral’s discursive field since his first work, Pedra do Sono (1940-1). These elements will clash with João Cabral’s poetics, previously based on the selection of voices and artistic practices consistent with his compositional method, with rigorous sorting values and elimination of any subjectivism. To understand this inconsistency, we propose studying the last part of the work Agrestes, entitled “A indesejada das gentes”, a place where the theme of death is recurrent, as well as the presence of enunciators to set out, even to reveal its feelings about aging and death. The analytical procedure should research the hypothesis of rupture of the inflexible tone of João Cabral’s speech that would follow in respect to the poetic project, a reverse path, starting from objective poetry in the direction of subjective poetry.

As figurações da morte e da memória na poética de Manoel de Barros

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Com a morte convivo

The documentary Com a Morte Convivo (With death I cohabit) shows people who work at or go to the Cemitério da Saudade (Nostalgia's Cemetery), located in Bauru, a city of the state of São Paulo. Investigating the relations between these characters and the cemetery brings out the discovery of both expressiveness and significance of image, silence and pause. The use of these elements intends to provide a sensory experience of the characters' world view

Folhetins teatrais e transferências culturais franco-brasileiras no século XIX: questões de uma edição da Semana Lírica de Martins Pena

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)