Alterações epigenéticas do gene RASSF1 e investigação de modulação gene-específica por non-coding RNA em linhagens celulares derivadas de carcinomas mamários

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise de SNPs do DNA mitocondrial em indivíduos residentes no estado do Espírito Santo para aplicação na Identificação Humana

Pós-graduação em Biotecnologia - IQ

Elementos de regulação e microRNAs envolvidos na modulação dos níveis de hemoglobina fetal em indivíduos portadores de beta-hemoglobinopatias

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da interação entre a via genética do microRNA156/squamosa promoter-binding protein-like e brassinosteróides durante o desenvolvimento de Arabidopsis thaliana

Pós-graduação em Ciências Biológicas (Genética) - IBB

Effects of electroacupuncture on experimental periodontitis in rats

Background: Acupuncture has shown the capability of modulating the immuno-inflammatory response of the host. This study aims to evaluate the effects of electroacupuncture (EA) on ligature-induced periodontitis in rats. Methods: Thirty-two animals were divided into four groups: 1) control; 2) experimental periodontitis (EP); 3) sham-treated (EP/EA-sham); and 4) treated with EA (EP/EA). For the EP groups, a ligature was placed around the right mandibular first molars at day 1. Sessions of EA or EA-sham were assigned every other day. For EA treatment, large intestine meridian points LI4 and LI11 and stomach meridian points ST36 and ST44 were used. EA-sham was performed in off-meridian points. Animals were euthanized at day 11. Histomorphometric and microtomographic analyses were performed. Immunolabeling patterns for the receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRAP) were assessed. Expressions of interleukin (IL)-1 beta, matrix metalloproteinase (MMP)-8, IL-6, and cyclooxygenase (COX)-2 messenger RNAs (mRNAs) were evaluated by quantitative reverse transcription-polymerase chain reaction. Data were analyzed statistically (P < 0.05, analysis of variance). Results: Histomorphometric and microtomographic analyses demonstrated that group EP/EA presented reduced alveolar bone loss when compared to group EP (P < 0.05). Reduced RANKL immunolabeling and fewer TRAP-positive multinucleated cells were observed in the EA-treated group in relation to group EP. No differences were observed in OPG expression among groups. EA treatment decreased the genic expression of IL-1 beta and MMP-8 (P < 0.05), increased the mRNA expression of IL-6 (P < 0.05), and did not modify the genic expression of COX-2 in animals with EP (P > 0.05). Conclusion: It can be concluded that EA reduced periodontal tissue breakdown and the expression of some proinflammatory mediators and a proresorptive factor in EP in rats.

MicroRNA-499 expression distinctively correlates to target genes sox6 and rod1 profiles to resolve the skeletal muscle phenotype in nile tilapia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The putative Leishmania telomerase RNA (leishTER) undergoes trans-splicing and contains a conserved template sequence

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transcrição cooperativa de genes ribossomais em Escherichia coli usando um modelo estocástico e dependente de sequência

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Expressão diferencial de microRNAs no câncer de próstata

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Utilização dos parâmetros físico-químicos e redes neurais artificiais na identificação dos méis de abelhas (Apis mellifera L.) produzidos no verão e inverno na microrregião de Campos de Jordão, São Paulo

The objective of this work was to typify, through physicochemical parameters, honey from Campos do Jordão’s microrregion, and verify how samples are grouped in accordance with the climatic production seasonality (summer and winter). It were assessed 30 samples of honey from beekeepers located in the cities of Monteiro Lobato, Campos do Jordão, Santo Antonio do Pinhal e São Bento do Sapucaí-SP, regarding both periods of honey production (November to February; July to September, during 2007 and 2008; n = 30). Samples were submitted to physicochemical analysis of total acidity, pH, humidity, water activity, density, aminoacids, ashes, color and electrical conductivity, identifying physicochemical standards of honey samples from both periods of production. Next, we carried out a cluster analysis of data using k-means algorithm, which grouped the samples into two classes (summer and winter). Thus, there was a supervised training of an Artificial Neural Network (ANN) using backpropagation algorithm. According to the analysis, the knowledge gained through the ANN classified the samples with 80% accuracy. It was observed that the ANNs have proved an effective tool to group samples of honey of the region of Campos do Jordao according to their physicochemical characteristics, depending on the different production periods.

Potenciais microRNAs circulantes como biomarcadores de Leucemia Mieloide Crônica - Fase Crônica: recém diagnosticada e tratada com mesilato de imatinibe

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização funcional da via miR159/SlyGAMYB-like ao longo do processo de frutificação em tomateiro (Solanum lycopersicum L.)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise cariotípica de duas espécies de cigarrinhas do gênero Mahanarva (Hemiptera, Cercopidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)