DNA barcoding reveals hidden diversity in the Neotropical freshwater fish Piabina argentea (Characiformes: Characidae) from the Upper Parana Basin of Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Genetics of neotropical fish: from chromosomes to populations

The Neotropical freshwater fish fauna is very rich-according to the most recent catalogue 71 families and 4,475 species have been described. However, only a small amount of general information is available on the composition of Neotropical marine fishes. In Brazil, 1,298 marine species have been recorded. General analysis of available cytogenetic and population genetic data clearly indicates research has been mainly concentrated on freshwater fishes. Thus, today, cytogenetic information is available for 475 species of Characiformes, 318 species of Siluriformes, 48 species of Gymnotiformes, 199 freshwater species that do not belong to the superorder Ostariophysi, and only 109 species of marine fishes. For the species studied, only about 6% have sex chromosomes and about 5% have supernumerary or B chromosomes. A review of the cytogenetic studies shows that these data have provided valuable information about the relationships between fish groups, the occurrence of cryptic species and species complexes, the mechanism of sex determination and sex chromosome evolution, the distribution of nucleolus organizer regions, the existence supernumerary chromosomes, and the relationship between polyploidy and evolution. In relation to populations in Neotropical marine waters, the studies have shown the presence of cryptic species, which has important implications for fishery management. Different levels of genetic structuring can be found among Neotropical freshwater migratory fish species. This raises important implications for fish population genetic diversity and consequently its sustainable utilization in inland fisheries and aquaculture, specifically for conservation of ichthyo-diversity and survival.

Molecular systematics of the armored neotropical catfish subfamily Neoplecostominae (Siluriformes: Loricariidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Isolation and characterization of microsatellite loci in the Neotropical fish Astyanax altiparanae (Teleostei: Characiformes) and cross-species amplification

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Aquatic insects as the main food resource of fish the community in a Neotropical reservoir

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Attractiveness of different sweet and fatty substances to three neotropical tramp ant species (Hymenoptera : Formicidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Occurrence of infection by Platynosomum illiciens (Braun, 1901) in captive neotropical primates

Platynosomum illiciens (Trematoda, Plagiorchida) is a trematode parasite reported in felids and falconiforms. It was identified in the gall bladder of eight captive neotropical necropsied primates from the National Primate Center (CENP), Ananindeua, State of Para, Brazil. This is the first description of Platynosomum illiciens as a parasite of primates.

Natural history of the coral snake Micrurus Pyrrhocryptus Cope 1862 (Elapidae) from semideciduous forests of western Brazil.

Coral snakes in the genus Micrurus are widely distributed in the Neotropics, with more than 50 species already described in this region. They are primarily ground or leaf litter dwellers, feed on snakes or other elongate vertebrates, and tend to reproduce during the rainy season. We present data on the biology of Micrurus pyrrhocryptus from two semideciduous forests of Mato Grosso do Sul state, western Brazil. Two snake species were consumed: the dipsadid Sibynomorphus lavillai and the typhlopid Typhlops brongersmianus. Sexual dimorphism is marked in M. pyrrhocryptus. with males attaining larger sizes and having longer tails. Activity was concentrated in the wet season, when reproduction occurs. The defensive behavior in this species is similar to that displayed by other Micron's, although less pronounced.

Endoparasites new to the Neotropical freshwater turtle, Mesoclemmys vanderhaegei (Bour 1973) (Testudines, Chelidae), from central Brazil.

Information on endoparasites infecting Neotropical turtles is scarce. The present paper reports the occurrence of endoparasites in three adult individuals of Vanderhaege's Toad-Headed Turtle (Mesoclemmys vanderhaegei). The records include an undescribed nematode species in the genus Spiroxys, not previously reported for the family Chelidae, and the first South American record of a monogenetic trematode of the genus Neopolystoma.

Population and reproductive biology of the crab Uca burgersi (Crustacea : Ocypodidae) in three subtropical mangrove forests

Population and reproductive biology were studied in three populations of the crab Uca burgersi Holthuis, 1967, in the Indaia, Cavalo and Ubatumirim mangrove forests (Ubatuba, São Paulo State, Brazil). Crabs were collected during low tide (August 2001 through July 2002), by digging the sediment, with a standard capture effort (two persons for 30 min.). Carapace width was measured, and gonad developmental stage was recorded from all specimens. U. burgersi was most abundant in the Cavalo mangrove, where the largest mate was found. Juvenile crabs were found year-round at all three sites. However, the ratio of ovigerous females was very low, even null in the Cavalo mangrove. The gonad development rate indicated that U. burgersi was reproducing continuously, but more intensively during spring and summer, with recruitment occurring in winter. The synchrony between the populational and reproductive biology in the three areas showed that local features were not the limiting factors. It is suggested that this species is a habitat generalist.

Structural and biological characterization of three novel mastoparan peptides from the venom of the neotropical social wasp Protopolybia exigua (Saussure)

The venom of the Neotropical social wasp Protopolybia exigua(Saussure) was fractionated by RP-HPLC resulting in the elution of 20 fractions. The homogeneity of the preparations were checked out by using ESI-MS analysis and the fractions 15, 17 and 19 (eluted at the most hydrophobic conditions) were enough pure to be sequenced by Edman degradation chemistry, resulting in the following sequences:Protopolybia MPI I-N-W-L-K-L-G-K-K-V-S-A-I-L-NH2 Protopolybia-MP II I-N-W-K-A-I-I-E-A-A-K-Q-A-L-NH2 Protopolybia-MP III I-N-W-L-K-L-G-K-A-V-I-D-A-L-NH2All the peptides were manually synthesized on-solid phase and functionally characterized. Protopolybia-MP I is a hemolytic mastoparan, probably acting on mast cells by assembling in plasma membrane, resulting in pore formation; meanwhile, the peptides Protopolybia-MP II and -MP III were characterized as a non-hemolytic mast cell degranulator toxins, which apparently act by virtue of their binding to G-protein receptor, activating the mast cell degranulation. (C) 2004 Elsevier Ltd. All rights reserved.

Structural and biological characterization of two novel peptides from the venom of the neotropical social wasp Agelaia pallipes pallipes

The venom of the neotropical social wasp Agelaia pallipes pallipes was fractionated by RP-HPLC resulting in the elution of seven fractions; the last two were re-fractionated under RP-HPLC by using isocratic elution conditions and the purity of the fractions were confirmed by using ESI-MS analysis. Both fractions are constituted of peptide components, which were sequenced by Edman degradation chemistry, resulting in the following sequences:Protonectin I-L-G-T-I-L-G-L-L-K-G-L-NH2Agelaia-MP I-N-W-L-K-L-G-K-A-I-I-D-A-L-NH2Both peptides are manually synthesized on solid-phase and functionally characterized by using Wistar rats cells. Protonectin is a non-hemolytic chemotactic peptide for polymorphonucleated leukocytes (PMNL), presenting some mast cell degranulating activity and potent antimicrobial action both against Gram-positive and Gram-negative bacteria. Agelaia-MP was characterized as a hemolytic mast cell degranulator toxin, presenting a poor antimicrobial action and no chemotaxis for PMNL. (C) 2004 Elsevier Ltd. All rights reserved.

Soldier head morphology of the Neotropical termites: Embiratermes festivellus Silvestri and Spinitermes brevicornutus (Desneux) (Isoptera, Termitidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

MRT Letter: A Novel Tegumental Gland in Female Imagoes of the Neotropical Termite Cornitermes cumulans (Isoptera, Termitidae, Syntermitinae)

In general, the exocrine glands of social insects are structures involved in the chemical communication associated with social life. Here, we report the discovery of an unknown tegumental gland that is present in the female imagoes of Cornitermes cumulans and occurs next to the well-developed tergal glands that have previously been described. The tegumental glands release their secretion in the intersegmental membrane and are composed of bicellular units, a secretory cell and a canal cell, that are closely located to the epidermal cells in the inferior part of the eighth and ninth tergites. The ultrastructure of the glandular cells showed abundant smooth endoplasmic reticulum, suggesting that the secretion may be pheromonal, although its function is still unknown. These exocrine structures are facing the tergal glands, and we hypothesized that they act synergistically with the tergal glands to generate short-range attraction during tandem behavior. Microsc. Res. Tech. 73: 1005-1008, 2010. (C) 2010 Wiley-Liss, Inc.

Hyperalgesic and edematogenic effects of peptides isolated from the venoms of honeybee (Apis mellifera) and neotropical social wasps (Polybia paulista and Protonectarina sylveirae)

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