Bio-safety technology in production of bovine herpesvirus type 5 (BoHV-5) using an alternative serum-free medium

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Myogenin, MyoD and IGF-I regulate muscle mass but not fiber-type conversion during resistance training in rats

The purpose of this study was to test the hypothesis that skeletal muscle adaptations induced by long-term resistance training (RT) are associated with increased myogenic regulatory factors (MRF) and insulin-like growth factor-I (IGF-I) mRNA expression in rats skeletal muscle. Male Wistar rats were divided into 4 groups: 8-week control (C8), 8-week trained (T8), 12-week control (C12) and 12-week trained (T12). Trained rats were submitted to a progressive RT program (4 sets of 10-12 repetitions at 65-75% of the 1RM, 3 day/week), using a squat-training apparatus with electric stimulation. Muscle hypertrophy was determined by measurement of muscle fiber cross-sectional area (CSA) of the muscle fibers, and myogenin, MyoD and IGF-I mRNA expression were measured by RT-qPCR. A hypertrophic stabilization occurred between 8 and 12 weeks of RT (control-relative % area increase, T8: 29% vs. T12: 35%; p>0.05) and was accompanied by the stabilization of myogenin (control-relative % increase, T8: 44.8% vs. T12: 37.7%, p>0.05) and MyoD (control-relative % increase, T8: 22.9% vs. T12: 22.3%, p>0.05) mRNA expression and the return of IGF-I mRNA levels to the baseline (control-relative % increase, T8: 30.1% vs. T12: 1.5%, p<0.05). Moreover, there were significant positive correlations between the muscle fiber CSA and mRNA expression for MyoD (r=0.85, p=0.0001), myogenin (r=0.87, p=0.0001), and IGF-I (r=0.88, p=0.0001). The significant (p<0.05) increase in myogenin, MyoD and IGF-I mRNA expression after 8 weeks was not associated with changes in the fiber-type frequency. In addition, there was a type IIX/D-to-IIA fiber conversion at 12 weeks, even with the stabilization of MyoD and myogenin expression and the return of IGF-I levels to baseline. These results indicate a possible interaction between MRFs and IGF-I in the control of muscle hypertrophy during long-term RT and suggest that these factors are involved more in the regulation of muscle mass than in fiber-type conversion. © Georg Thieme Verlag KG Stuttgart · New York.

Adaptações morfofuncionais e respostas moleculares do músculo esquelético de ratos submetidos ao treinamento aeróbio

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O papel do fator de crescimento semelhante à insulina-l sobre os efeitos deletérios do choque térmico em oócitos bovinos no estádio de vesícula germinativa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Substituição do uso de soro fetal bovino na manutenção do cultivo de células CER infectadas pelo vírus da doença infecciosa da bursa de Fabrícius

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Adaptações morfofuncionais e respostas moleculares do músculo esquelético de ratos submetidos ao treinamento resistido

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do fator de crescimento IGF-I sobre a maturação in vitro de oócitos caninos (Canis familiaris): avaliação da maturação nuclear e citoplasmática

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O efeito do fator de crescimento semelhante à insulina-I em oócitos de vacas Bos indicus e Bos taurus expostas ao estresse térmico un vitro

Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

Linfoma de Hodgkin: características anátomo-clínicas e análise de novo biomarcador (IMP3)

Pós-graduação em Patologia - FMB

Effect of feed restriction on body composition and metabolism of goats of different genders

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência do fator de crescimento semelhante à insulina I (IGF-I) adicionado ao meio fluido sintético de tuba uterina (SOF) sobre a maturação in vitro de oócitos caninos (Canis familiaris)

The follicular growth and oocyte maturation knowledge are very important to the development and improvement of new biotechnologies such as in vitro fertilization and somatic cell nuclear transfer. In order to the necessity of clarify the basic mechanisms related to canine oocyte maturation, this investigation focuses on the evaluation of the effect of insulin-like growth factor-i (IGF-I), added to synthetic oviductal fluid medium (SOF) on the in vitro maturation of domestic dog oocytes. Thirty-seven bitches undergoing ovariohysterectomy for castration or due to pathological conditions of the uterus were selected as oocytes' donors (n=875). The oocytes were allocated in the following groups: MO (stained in the collection's time), Control (72h in SOF) and Experimental (72h in SOF plus 100 ng IGF-I). After 72 hours of maturation the oocytes' nuclear status were assessed by Hoechst 33342 dye. The best results in terms of oocyte harvest were observed in those juvenile donors,, females in estrus, nuliparous and pure breeds. No significant differences were observed between treatments control (SOF) or experimental (IGF-I).

Histochemical and ultrastructural analysis of hepatic glycogen and collagen fibers in alloxan-induced diabetic rats submitted to long-term physical training

Alterations in liver functions are common among diabetic patients, and many symptoms in the liver have been reported, including changes in glycogen stores and in the amount of collagen fibers. The practice of physical training and its morphological effects in this organ, however, are scarcely studied. In order to observe the morphological effects of alloxan-induced diabetes and the alterations arising from the practice of long-term chronic physical training in the liver, samples were collected and processed, and then analyzed by means of the histochemical techniques Periodic Acid-Schiff and Picrosirius-Hematoxylin, and ultrastructural cytochemical test of Afzelius. Through evaluation of the tissue, it was observed a drastic reduction in hepatic glycogen stores of sedentary diabetics, recovered in trained diabetic rats. Furthermore, it was detected a decrease in the content of perisinusoidal collagen fibers in the diabetic liver, also recovered due to the development of a training protocol. On ultrastructural level, cytochemical analysis confirmed the loss of glycogen and the recovery obtained by training. In conclusion, the practice of a long-term chronic physical training protocol may be considered an important assistant in the treatment of diabetes, mitigating the occurrence of possible damages to liver tissue. © 2011 Elsevier Ltd.

Differential expression of members of the IGF system in OPU-derived oocytes from Nelore (Bos indicus) and Holstein (Bos taurus) cows

The insulin-like growth factor (IGF) system is related to quality of oocytes and embryos. The aim of this study was to investigate the mRNA levels of IGF1 and IGF2 and their receptors, IGFR1 and IGFR2, as well as IGFBP2, IGFBP4, and PAPP-A in oocytes from Nelore compared to Holstein cows. Pools of oocytes (20 oocytes/pool) from Nelore (n=8 pools) and Holstein (n=4 pools) were obtained via ovum pick-up (OPU, 10 sessions) and cumulus cells and zona pellucida were removed. The pools were submitted to total RNA extraction. Expression of members of the IGF system was assessed by real time RT-PCR. The mRNA expression of IGF1 and IGF2, IGFR1 and IGFR2, IGFBP2 and IGFBP4 was significantly higher (P<0.01) in oocytes from Holstein whereas the expression of PAPP-A was significantly higher (P<0.05) in oocytes from Nelore cows. The high PAPP-A expression and the low expression of IGFBP2 and IGFBP4 are associated with more efficient degradation of IGFBPs, which results in greater bioavailability of IGF in Nelore oocytes when compared to the Holstein. © 2013 Elsevier B.V.

Avaliação do padrão de metilação da DMR (Differentially Methylated Region) dos gnes IGF2 e H19 em carcinomas uroteliais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

O uso da somatotropina bovina recombinante em reprodução bovina

The recombinant bovine somatotropin (rbST) at first, had its widespread use in dairy cows in order to increase milk production. Currently, it has been studied frequently and use their influence both in bovine milk, such as cutting. Its production has been an evolution to the science, using bacteria to produce recombinant DNA. Most authors that have studied and obtained positive results, such as increasing the number of ovarian follicles larger than five millimeters, among others. Its action takes place directly on the ovary, follicles, corpus luteum, the granulosa cells, oviduct, myometrium, endometrium and placenta, where they were found receptors, or indirectly through the release of insuline like growth factor-1 (IGF-1). Therefore, the objective of this work is to explain the importance of bST in bovine as well as the usefulness of this, its mechanism of action and the benefits it can bring when combined with other biotechnology, such as superovulation, embryo transfer, synchronization of estrus, and others