Diabete moderado induzido em diferentes fases da vida de ratas Wistar: repercussões na reprodução materna e na freqüência de anomalias fetais

Experimental models are necessary to elucidate pathophysiological mechanisms not yet understood in humans. To evaluate the repercussions of the diabetes, considering two methodologies, on the pregnancy of Wistar rats and on the development of their offspring. In the 1st induction, female offspring were distributed into two experimental groups: Group streptozotocin (STZ, n=67): received the β-cytotoxic agent (100mg STZ/kg body weight - sc) on the 1st day of the life; and Non-diabetic Group (ND, n=14): received the vehicle in a similar time period. In the adult life, the animals were mated. After a positive diagnosis of pregnancy (0), female rats from group STZ presenting with lower glycemia than 120 mg/dL received more 20 mg STZ/kg (ip) at day 7 of pregnancy (2nd induction). The female rats with glycemia higher than 120mg/dL were discarded because they reproduced results already found in the literature. In the mornings of days 0, 7, 14 and 21 of the pregnancy glycemia was determined. At day 21 of pregnancy (at term), the female rats were anesthetized and killed for maternal reproductive performance and fetal development analysis. The data were analyzed using Student-Newman-Keuls, Chi-square and Zero-inflated Poisson (ZIP) Tests (p<0.05). STZ rats presented with increased rates of pre (STZ=22.0%; ND=5.1%) and post-implantation losses (STZ=26.1%; ND=5.7%), reduced rates of fetuses with appropriate weight for gestational age (STZ=66%; ND=93%) and reduced degree of development (ossification sites). Conclusion: Mild diabetes led a negative impact on maternal reproductive performance and caused intrauterine growth restriction and impaired fetal development

Avaliação da exposição ao propionato de testosterona em ratas fêmeas in utero e lactação

It is known that exposure to substances in the environment can contribute to various reproductive disorders, especially if such exposure occurs during critical periods of development such as the intra-uterine and postnatal. The female reproductive system may be the target of androgens, both as a result of exposure to environmental chemicals, or by pathological conditions (polycystic ovary syndrome or congenital adrenal hyperplasia).Usually, little attention is given off in relation to the study of androgenic effects in the female reproductive axis. This study aims to evaluate the effects of exposure to androgens on the development, structure and reproductive function in rats whose mothers were exposed to testosterone propionate from gestational day 12 (DG12) after weaning - postnatal day 21 (DPN21) . For this purpose, pregnancy rats were divided into four groups: a control group that received corn oil (vehicle) and three groups receiving testosterone propionate in doses of 0.05 mg / kg / day, 0.1 mg / kg / day and 0.2 mg / kg / day, all under the same experimental conditions. The possible effects of exposure were assessed using reproductive parameters, such as a measure of anogenital distance, count areolas / nipples, age at vaginal opening and first estrus (puberty indicative installation), weight and histological evaluation of the reproductive organs ( uterus and ovaries), weight of the kidneys, liver and pituitary hormone levels, regularity of the estrous cycle, sexual behavior and fertility. Such analysis is important in understanding the effects of androgen exposure on the female genital system, especially on the reproductive potential, and processes that may involve morphofunctional changes. In these experimental conditions, it is concluded that treatment with PT caused reduction in body weight and initial masculinization in females without cubs, however, commit further sexual development

Reparação de enxertos ósseos autógenos recobertos ou não por membrana de ptfe-e em ratas ovariectomizadas. Estudo histológico e histomorfométrico

Abstract : The objective of this study was to evaluate quantitatively and to describe qualitatively the process of bone repair in the interface of receptor bed and graft autogenous bone block with or without a e-PTFE membrane, in osteopenia induced rats. To this end, we used forty-eight Wistar rats weighing about 300g, in which, with the aid of 4.1 mm trephine a graft was removed from the parietal bone and fixed to the surface of the left mandibular ramus. The animals were randomly divided into four experimental groups: Group 1 (n=12): SHAM operated and autogenous bone graft only; Group 2 (n=12): SHAM and autogenous bone graft covered by e-PTFE membrane; Group 3 (n=12): ovariectomized rats (OVX) and autogenous bone graft only; Group 4 (n=12): OVX and autogenous bone graft covered by e-PTFE membrane. The animals in each group were sacrificed at three time periods: 21, 45 and 60 days, each time with 4 animals per group. The specimens were decalcified and included, the sections were stained with HE and subjected to histological and histomorphometric analysis in light microscopy. The results of the ANOVA showed that the variables on the condition (OVZ and SHAM), and the time (21, 45 and 60 days) were statistically significant, and can be established with the Tukey test (5%) that the period 21-day differs significantly from the periods of 45 and 60 days, which did not differ among themselves. The descriptive histological analysis showed integration of the graft in all animals. It was concluded that the initial integration of the graft bed was negatively affected in the presence of induced osteopenia, and that the use or not of a e-PTFE membrane did not interfere in the process of integration

Efeito da administração de alendronato, a longo prazo, sobre as propriedades mecânicas do tecido ósseo, sua reparação e mineralização ao redor de implantes osseointegráveis. Estudo em ratas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação dos efeitos promovidos pela sibutramina, associada ou não à nicotina, nos tecidos reprodutivos de ratas albinas

Sibutramine is a drug recommended clinically for the treatment of obesity, but there are women that use the drug for maintenance of body weight. Many time this use occur associate to habit of tabagism, being the nicotine the main toxic compound in the cigarette. The goal of study was to evaluate the side effects promoted by sibutramine, associated or not to nicotine, in the reproductive tissue of adult female rats. Wistar animals (n =30), were distributed in the groups: a) Control A (0.3 mL of distilled water; oral); b) Sibutramine (15 mg/kg of body weight; oral); c) Control B (0.3 mL of saline solution; intraperitoneal); d) Nicotine (4.0 mg/kg of body weight; intraperitoneal); e) sibutramine + nicotine. The treatments were conducted during 30 consecutive days (single dose, daily). Sibutramine, associated or not to nicotine, affected the folliculogenesis and luteogenesis. There were significant alterations (p<0.05) in the thickness of uterine layers, considerate each treatment. In conclusion, the administration isolated of sibutramine or nicotine promoted deleterious effects in the reproductive tissues of female rats and these effects were potential in the group that received both drugs.

Avaliação da fosforilação da AKT e do conteúdo da proteína transportadora de glicose GLUT4 em tecido muscular esquelético de ratos adultos, proles de ratas com doença periodontal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Papel das espécies reativas de oxigênio na hiporreatividade de aortas à fenilefrina observada no final da prenhez de Ratas Espontaneamente Hipertensas (SHR)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da ovariectomia e da reposição hormonal no reimplante dentário imediato: análise histomorfométrica e imunoistoquímica em ratas

Pós-graduação em Odontologia - FOA

Análise hormonal, imunolocalização e quantificação dos receptores de andrógenos (AR) e estrógenos (ER-α E ER-β) em ovário e útero de ratas submetidas a diferentes doses de decanoato de nandrolona: avaliação nos períodos pós-tratamento e pós-recuperação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da inalação da fumaça de cigarro na regeneração óssea guiada em enxertos autógenos onlay em ratas com deficiência estrogênica: estudo histomorfométrico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)