Isolamento de fungos produtores de enzimas pectinolíticas

Soil samples collected in the campus, UNESP, Araraquara, SP, were employed to isolate and characterize fungi strains with potential pectinolytic enzymes. These enzymes have arisen great interest due to its increasing application in the food industry. Two hundred forty six strains were isolated based on the appearance of colony on PDA medium, morphology (septate mycelia, nonseptate conidiophore, black conidia, and clublike spore-bearing head), after 48 h of growth at 30°C. Strains were selected in solid medium containing pectin citrus as sole carbon source and 0.5% rutenium red. The characterization of pectinolytic production was performed in solid culture and batch fermentation medium containing pectin citrus. The enzyme pectinolytic production was evaluated at 30°C, without agitation in 100 mL of medium containing 2% pectin citrus, 0.2% ammonium sulphate, 0.2% magnesium sulphate, and 0.05% potassium phosphate. The maximum pectinolytic activity (15U/mL) was observed in the medium after Aspergillus sp CFCF-0492 growth, while Aspergillus sp CFCF-CC1 showed the higher level of the final biomass. The pectinolytic activity is more preserved when the fungi-spores were maintained in agar-Czapeck medium.

Avaliação de interpoladores estatísticos e determinísticos na estimativa de atributos do solo em agricultura de precisão

The obtaining of the correct space distribution for attributes of the soil is relevant in the agricultural planning, in what concerns to the installation and maintenance of the cultures. The objective of that work was to compare statistical interpolation methods (ordinary krigagem) and deterministic methods (inverse square distance) in the estimate of CTC and V% in a distrophic yellow-red Latossolo. The study was accomplished in the State of Experimental Hands on of the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (INCAPER), in an irregular grading with 109 points. The data were collected in the layer of 0 - 0,20 m in the projection of the cup of the plants, in the superior part of the slope. The performance of the interpolators was obtained and compared using the criterion of the medium mistake. The observations are dependent in space until a maximum reach of 14,1 m, considering the isotropy. IDW presented larger mistake in the estimate of the data; however its difference in relation to KRIG was small for both variables.

Sementes de feijão-caupi (Vigna unguiculata L. (Walp), tratadas com extrato de folhas de nim (Azadirachta indica A. Juss.) avaliação da germinação e da incidência de fungos

This study aimed to evaluate the effect of aqueous extract of neem on germination and fungi incidence on seeds of three cultivars (Serrinha, BR 17 and Maranhão) of cowpea. Neem leaves were dryed, crushed and prepared dilutions of 0.5; 1.0; 2.0, 4.0 g dm -3and control. The fungi incidence was evaluated by the test filter paper and germination according to the Rules for Seeds Testing (Regras para Análise de Sementes). In the three cultivars analyzed, reduction in the incidence of Aspergillus sp and Fusarium sp was observed. In relation to the influence of extracts of neem leaves on seed germination, significant effect of extract in Maranhão cultivar was observed, where all concentrations differed from the control, and propovided a considerable increase in the percentage of normal seedlings. It was concluded that the leaf extract of neem was effective in controlling Aspergillus sp, Fusarium sp , Phoma sp and Macrophomina phaseolina at different concentrations in different cultivars and seed germination was stimulated for the Maranhão cultivar.

Situação da agricultura irrigada no Brasil de acordo com o censo agropecuário 2006

Knowledge about irrigated agricultural area and its geographical distribution are of great importance to water resources management. Evolution of irrigated area according to the irrigation method allows an identification of rational water technologies. The aim of this work was to analyze the information presented in the agricultural census of 1995/1996 and of 2006. Brazil has an area of 4.45 million cultivated hectares with irrigation. It is noticed that according to the 1995/1996 census, the irrigated area increased 1.3 million hectares, a growth of 42%. The Southeast region surpasses the South region which had, in 1995/96, the largest irrigated area. The Northeast region is the third irrigated agricultural area with 22.12 %, while the Central-west and the Northern region together sum 14.7%. According to the agricultural census 2006 classification, sprinkler irrigation (without center pivot) method is the most used,followed by flood, center pivot, other methods, drip and furrow, representing 35.3%, 24.4%, 18.9%, 8.3%, 7.4% and 5.8%, respectively.

A moderna agricultura no final do século XIX em São Paulo: algumas propostas

Esta pesquisa se insere dentro da história da agricultura no Brasil, especialmente no Estado de São Paulo, a partir da segunda metade do século XIX, início do século XX. As propostas de modernização agrícola giravam em torno de diferentes assuntos, mas sempre interligados, como: diversificação da agricultura, adubação química e natural, cultura intensiva do solo, mão de obra, povoamento e colonização, instalação de núcleos coloniais dentro dos moldes da moderna agricultura então desejada, instrução agrícola por meio de campos de experiências e demonstração, ensino agrícola para diferentes graus, a mecanização da lavoura, o que era chamado, na época, de Moderna Agricultura, em substituição ao que era considerado como agricultura rotineira ou atrasada, praticada desde o início da agricultura no Brasil.

Aspectos Históricos e organizacionais da agricultura familiar no desenvolvimento da região Nova Alta Paulista

Nova Alta Paulista region, with strong aptitude for agribusiness, is one of the poorest in the state of São Paulo. However, the development of agribusiness productive chains in the region is very heterogeneous. The study aimed at analyzing how the historical background and organizational arrangements in family farming have contributed to regional development by promoting improvements in social welfare at Nova Alta Paulista region. To this end, we analyzed the case of the Associação Agrícola de Junqueirópolis - AAJ, an organization that has been successful in the search for alternative of agricultural production. Integrated to local and global market, this organization has ensured the maintenance of many farmers and generated positive externalities for the entire region. Thus, this case shows the organizational arrangement based on cooperation can be an alternative for family farmers achieve higher levels of welfare and development.

Estabelecimento de fungos nematófagos aplicados no campo para o controle dos nematoides-chaves dos citros no Brasil

Monitoring the survival of nematophagous fungi is needed to establish periods of reapplication of formulations of nematophagous fungi to control the citrus nematode in the field. We monitored the survival of fungi: Arthrobotrys robusta, A. oligospora, A. musiformis Dactylella leptospora and Monacrosporium eudermatum in plots treated with 1, 2, 4, 6 liters of the formulation of fungi/plant or witness without the application, during the period of nine months with the first assessment six months after application and the other with intervals of three months after the first evaluation. The fungus D. leptospora was found only in the evaluation of 6 months after treatment application, indicated a short survival time in the soil. However, the isolated A. robusta, A. musiformis and A. oligospora were recovered in all evaluations and especially in plots treated with higher doses of the formulation and witness. Monacrosporium eudermatum was recovered in all experimental periods and even in assessing the witness portion of nine months after application. The fact of the presence of species of Arthrobotrys and M. eudermatum in control plots indicates that native species that were already orchard.

Fungos endofíticos: Uma fonte inexplorada e sustentável de novos e bioativos produtos naturais

Endophytic fungi are a rich source of new and biologically active natural products. They colonize a relatively unexplored ecological habitat and their secondary metabolism is particularly active, presumably due to metabolic interactions with their hosts. In the course of our continuing investigations for new and bioactive compounds from endophytic fungi from brazilian flora Alibertia macrophylla, Caseria sylvestris, Ocotea corymbosa, Cassia spectabilis, Piper aduncum, Cryptocaria mandioccana, Xylopia aromatica and Palicourea marcgravii were investigated. Forty two natural products were isolated and their structures were established on the basis of comprehensive spectral analysis, mainly using 1D and 2D NMR experiments. The compounds were tested in their antifungal, antioxidant, anticholinesterasic and anticancer activities.

Velocidade de absorção do glufosinate e seus efeitos em plantas daninhas e algodão

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Semeadura de braquiária em consorciação com milho em diferentes espaçamentos na integração agricultura-pecuária em plantio direto

Pós-graduação em Agronomia - FEIS

Toxicidade aguda e risco ecotoxicológico do fipronil para o guarú (Poecilia reticulata) e dissipação no ambiente aquático

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Descrição da diversidade de fungos associados a formigas Attini por método independente de cultivo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Agricultura tradicional e a manutenção da agrobidiversidade em comunidades rurais do município de Santo Antonio de Leverger - MT

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prospecção de enzimas de degradação de materials vegetal em fungos endofíticos

Pós-graduação em Biotecnologia - IQ