77 resultados para ELISA competitivo
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Introduction: The myofascial pain syndrome (SDM) is one of the most common causes of musculoskeletal pain. One of the possible treatments for SDM is the type of physiotherapy myofascial manipulation. Objective: This study aimed to analyze the effect of manipulative technique with myofascial pain threshold before and after applying the technique in athletes during competition period. Methods: Participated in the study 62 subjects of both genders, aged between 14 and 38 (19.64 + 4.89), who had myofascial pain syndrome, 32 oh the treatment group and 30 divided equally between control group and the placebo group. All were athletes and operated by the Department of Sport and Leisure in the city of Marilia – SP and were in competitive period. The volunteers were evaluated according to their musculoskeletal symptoms to prove the necessity of performing the technique of myofascial manipulation. Confi rmed the need to assess the pressure pain threshold (LDP) using a digital dynamometer. After the measurement, patients underwent treatment or using the technique of myofascial manipulation, or a sliding surface for the placebo or no treatment for the control group followed by the immediate reassessment of the LDP. Results: The results were normalized by Kolmogrov-Smirnov test (KS). Through the ANOVA test found no differences between the initial LDP thresholds between groups. To compare pre and post LDP of the three groups we used the paired t test. Signifi cant difference (p=0.0001) between the values of pain threshold before and after application of myofascial manipulation for the treated group and not signifi cant for the control group (p=0.45) and placebo (p=0.16). Conclusion: We conclude then that the myofascial manipulation technique is able to increase pain threshold after micro-musculoskeletal injuries in athletes in competitive period.
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A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations. (C) 2014 Elsevier B.V. All rights reserved.
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The theme approached relates contemporaneous organizational competitive scenario to draw parallels between the organizational structure, Knowledge Management and Public Relations. Many aspects are complementary and can be grouped, enabling the idea of verifying the possibility of a Public Relations work like a manager of Knowledge Management. The objective of this study focuses in analyzing the administration ways of the organizational environment to verify the best kind of structure for the competitive development pattern, then we sought the meaning of Knowledge Management and their results to draw a parallel between the image of the Knowledge Management process manager and the Public Relations professional. The methodology chosen was bibliographic research, by which we noticed the theme relevance, the proposal validity and build a convergence between the skills of a person responsible for managing processes in Knowledge Management and the capabilities of a Public Relations professional. This way adopts a human feature to the managing process, respecting the technical-informational scenario of this area
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Atualmente a busca da mulher pelo esporte vem crescendo, juntamente com o seu desempenho e a participação em competições. No futebol de campo não é diferente e a adesão das mulheres, ou seja, o número de atletas do sexo feminino vem se tornado expressivo nos últimos anos. A motivação é o combustível para o atleta, seja ele homem ou mulher, independente da modalidade. Por isso não pode prescindir dela. “É através desse que o atleta vai conseguir empenhar-se, dedicar-se e até superar obstáculos dentro do meio esportivo” (MARQUES, 2003, p.99). A motivação é a totalidade daqueles fatores, que determinam a atualização de formas de comportamento dirigido a um determinado objetivo e é caracterizada como um processo ativo, intencional e dirigido a uma meta, o qual depende da interação de fatores pessoais (intrínsecos) e ambientais (extrínsecos). (SAMULSKI, 2002). Contudo o objetivo do presente estudo é verificar o quão importante e influente é o trabalho motivacional na preparação de equipes de futebol feminino para competições. Diante do exposto, estudaremos, no meio de uma revisão de literatura, a importância da motivação em atletas de equipes de futebol feminino
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O presente estudo tem como objetivo reunir os dados existentes sobre a área da futura Estação Ecológica Santa Elisa, localizada no Centro Experimental Central do Instituto Agronômico (IAC), em Campinas – SP. Assim, foram reunidos dados sobre os aspectos bióticos (fauna e flora) e abióticos (clima, geologia e geomorfologia, hidrografia e solos), resultando em uma caracterização da área como forma de subsídio para o plano de manejo. Através de saídas a campo foram levantados problemas que prejudicam a caracterização de uma estação ecológica, surgindo assim um segundo objetivo, adequar a área da futura unidade para o melhor enquadramento em tal categoria. Foram então elaborados projetos para melhorar o estado de conservação da futura unidade, sempre levando em consideração a falta de recursos. Sendo assim recomenda-se parcerias com universidades para que dessa forma se consiga implantar os projetos e realizar pesquisas de forma mais econômica. Embora o IAC tenha um histórico de pesquisas voltado apenas para a agricultura, a preocupação ambiental vem aumentando e a criação dessa unidade de conservação garante que se continue preservando os remanescentes presentes no IAC mesmo após a mudança de sua gestão.
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O contexto esportivo é repleto de muitas manifestações psicológicas. Para se ter sucesso é necessário que ocorra uma grande preparação destes atletas, que devem estar preparados fisicamente, tecnicamente e taticamente para responder às necessidades do esporte, buscando sempre a vitória e a melhor performance nas competições desportivas. A Psicologia do Esporte vem desenvolvendo programas de treinamento psicológico envolvendo técnicos, treinadores e atletas na busca de melhor desempenho nas competições. Hoje em dia o voleibol é um dos esportes mais praticados e assistidos no mundo inteiro, sendo no Brasil o segundo esporte mais praticado. O estado emocional se altera tanto em um período pré-competitivo, onde a incerteza de futuros acontecimentos provoca uma ansiedade, tensão e certo desconforto, quanto no momento da competição e também no período póscompetitivo. O sentimento de vergonha também é manifestado em muitas ocasiões. Várias são as definições encontradas para essa emoção: sentimento penoso de desonra; humilhação; rebaixamento; sentimento de insegurança provocado pelo medo do ridículo; embaraço; indignidade; timidez; acanhamento; sentimento de desconforto, entre outras. . A pesquisa buscou analisar teórica e praticamente as alterações emocionais de atletas em suas funções esportivas. Foi realizada com uma equipe de voleibol feminino, onde se aplicou um questionário fechado, a 21 atletas, com idades entre 13 e 16 anos. Verificou-se com que freqüência as manifestações dos sentimentos de vergonha e ansiedade ocorriam e possivelmente prejudicavam o desempenho das atletas. Após análise dos resultados pode-se concluir que as manifestações de ansiedade foram mais evidenciadas se comparadas as de vergonha. Notamos que, as preocupações quanto ao desempenho e aos erros que podem ser cometidos são muito evidenciados na equipe. A vergonha-meta... (Resumo completo, clicar acesso eletrônico abaixo)
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Pós-graduação em Medicina Veterinária - FCAV
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As Artes Marciais tiveram, em sua maioria, origem no Oriente, em meados do século XIX, principalmente Japão, Índia e China. No inicio essas Artes apresentavam caráter de defesa pessoal e militar. No entanto a intensificação do contato com o Ocidente e a evolução das armas de fogo, fez com que as lutas que utilizavam apenas o corpo caíssem em desuso, passando a ser usada como uma modalidade esportiva e competitiva. Atualmente tem adeptos no mundo todo que buscam avanços nos aspectos físicos (saúde), motores e psicológicos, ale de aprimorar o autodomínio, superar limites e melhorar o poder de concentração (KODOKAN, 1995). Dentre as modalidades mais difundidas pelo mundo, destacam-se o Karatê, o Kung-fu, o Jiu-Jitsu e o Judô, destas darei maior ênfase nas duas últimas. A Psicologia do esporte é uma área da psicologia que visa promover a saúde, a comunicação, as relações interpessoais, a liderança e a melhora do desempenho esportivo. Artes marciais são modalidades onde são encontrados altos níveis de estresse pelas mais variadas causas, sendo assim uma revisão bibliográfica foi realizada para buscar analisar as variáveis envolvidas e respostas de atletas das modalidades bem como os treinadores envolvidos
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Caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis, is a chronic contagious disease that affects small ruminants and still remains an important problem for many lamb-producing countries. Animals are considered clinically infected when occurs abscesses in superficial lymph nodes. Visceral or internal form can coexist which no apparent clinical signs of infection are seen. The best procedure to avoid spread of the disease is elimination of infected animals. However, as the chronic and subclinical nature of the infection of CLA alternative methods are required for detection and screening. In this study, we described the performance of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for diagnosis of CLA in asymptomatics sheep. Also, test culture and biochemical identification were achieved to confirm CLA infection. The serological diagnostic was performed in sheep symptomatics (n=50) and asymptomatics (n=374) from nine flocks. Analysis reported high positivity of 71% for ELISA in 85% of asymptomatic animal for CLA with a sensitivity of 88% and specificity of 31%. Results from ELISA test in asymptomatic animals against culture for caseous lymphadenitis were more specific (97%) and permitted to exclude healthy animals without symptoms. This study concluded that C. pseudotuberculosis infection could be widely disseminated in sheep flocks in Northwestern region of the state of São Paulo, Brazil and only one screening test is not enough. The association with indirect ELISA test and culture could better indicate the real problem of CLA in sheep flocks.
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This study compared the hematological and serological analysis of diagnosis of canine ehrlichiosis. The survey of Ehrlichia canis was performed through the evaluation of blood smears from 150 dogs. The serological test was performed on 12 samples selected by the platelet count (less than 170,000 platelets / uL). Serologic testing was performed with the Imunocomb kit - Dot-blot-ELISA. No cytoplasmatic inclusion characteristic of morula of E. canis was found in blood smears. In serologic testing, eight samples were positive for Ehrlichia canis, concluding that thrombocytopenia is an important hematological finding of ehrlichiosis diagnosis and the detection of Ehrlichia canis morulae is uncommon. The serological evaluation Dot-blot ELISA is an accurate and brief diagnosis method of canine ehrlichiosis, been the most appropriate to be used in veterinary practice routine.
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The aim of this study was to examine changes on anaerobic power after competitive period in professional soccer players. Twenty five male was evaluated before (PRE) and after (POS) competitive period. To assess anaerobic power was used running based on anaerobic sprint test (RAST), which were determined the maximum power (MAXP), medium power (MEDP), minimum power (MINP) and fatigue index (FI). The test was performed in the first (PRE) and the last (POS) training session of competitive period, wich lasted 20 weeks. There were no significant difference (p>0,05) in POS condition compared to PRE condition on MAXP (10,70 ± 0,95 vs 10,83 ± 0,87), MINP (8,48 ± 0,92 vs 8,28 ± 0,76), MEDP (9,52 ± 0,83 vs 9,41 ± 0,61) and FI (22,73 ± 7,48 vs 25,53 ± 8,79). There was no significant change on anaerobic power after a competitive period wich lasted 20 weeks in professional soccer players.
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Change on vertical jump after competitive period in professionals soccer players. Brazilian Journal of Biomotricity, v. 4, n. 2, p. 140-147, 2010. Soccer is a sport that demands different intensities of run, with decisive actions of a match being held in maximum intensity. Vertical jump test is widely used in soccer players due to the strong relationship with speed and agility. Futhermore, there are little information about change on vertical jump after the competitive season in soccer players. The aim of this study was to analyze change on vertical jump after the competitive season in professional soccer players. Took part in this study 21 male athletes (20.82 ± 3.16 years, 72.28 ± 8.74 kg and 179.91 ± 6.14 cm) subscribers to the 4th division of the Paulista championship of 2009. The competitive season had a duration of 20 weeks, with a total of 20 official matchs done. The test used was the counter-movement vertical jump (VJ), that was performed in the first (PRE) and last (POS) training session of the competitive period. After confirmation of data normality by Kolmogorov-Smirnov, the inferential analysis of the results of VJ between PRE and POS was performed using the paired t-test, considering the significance level of 5%. There was a significant increase (p<0,05) on VJ after the competitive period (PRE=54,19±4,46 and POS=57,94±5,23). According to the results of this study, it is possible to increase the performance of VJ in professional soccer players after the competitive period of 20 weeks duration.