Proliferação celular nos linfomas caninos

Lymphoma studies deals with morphological classification and immunophenotypic features and they have to be amplified for cellular kinetics evaluation. This evaluation can only be safely made, when the proliferative index are evaluated. In men the proliferation index have, most of the time, important influence in the neoplasia prognosis and treatment In this work it was used 40 canine lymphomas that were classified according to Kiel methods and immunophenotype was achieved with CD3 (T lymphocyte) and CD79a (B lymphocyte). Cellular proliferation was evaluated by AgNORs and Ki-67 (MIB-1) According to Kiel classification system, high grade lymphomas were more frequent and T and B lymphoma showed the same frequency.When cellular proliferation was evaluated, there was a significant difference between high grade and low grade lymphomas by AgNOR and Ki-67 (MIB-1) methods, but did not differ when comparing immunophenotype. Among high grade malignancy lymphomas the NORs medium number per cell nucleoli was 1.37± 0.32 and in low grade was 0.98± 0.36, concerning Ki-67 the positive cellular percentual was 43.19% ± 19.01, e 14.09% ± 11.74 in high and low grade, respectively.

Polissacarídeos de parede celular fúngica: purificação e caracterização

The cell wall is a rigid structure essential for the survival of fungi. A knowledge of its composition is therefore useful for the development of novel anti-fungal drugs. In this context, polysaccharides as main components of the fungal cell wall have been the subject of intense scientific study over the years. The information gained from the knowledge of the structure of these macrobiomolecules could therefore be valuable in elucidating the mechanisms of their biosynthesis in the cell walls of pathogenic fungi infecting plants and animals alike. Determination of the chemical structures of these polysaccharides (endo) is preceded by their extraction and purification. The extractions, generally lead to neutral and/ or alkaline soluble biopolymers in groups according to their solubilities. Mixtures of polysaccharides in these extracts can then be purified by a combination of chemical and chromatographic methods. Following purification, the polysaccharides, considered homogeneous, can be characterized structurally using conventional techniques of carbohydrate chemistry, such as hydrolysis, methylation analysis, and FT-IR, 13C- and 1H- NMR spectroscopy. This review surveys the main scientific literature that characterizes polysaccharides constituting the fungal cell wall.

Uso de expressões orais durante a implementação do recurso de comunicação suplementar e alternativa

The literature has discussed the importance of early implementation of augmentative and alternative systems with people with various disabilities. This discussion is related to concerns about language acquisition and development within the various expressive possibilities. Researchers advise that starting intervention early through resources and procedures using augmentative and alternative communication does not impede speech acquisition and development. This paper aimed to describe oral expressive abilities during the implementation of augmentative and alternative communication with a student with cerebral palsy. An 11-year-old student with cerebral palsy participated in the augmentative and alternative communication program for two years. Twelve sessions were selected during the first year of the intervention. The sessions were filmed and the augmentative and alternative communication resource procedures were transcribed. The categories of analysis were defined as verbal expression; nonverbal expression and verbal and nonverbal expression associated. The results of this study identified that augmentative and alternative communication resources supported the use of verbal expression such as vocalizations, words and unintelligible oral expressions.

Lipoperoxidação, perda de viabilidade celular e diminuição da liberação de IL-8 de neutrófilos humanos na presença de corpos cetônicos

Type-1 diabetes patients suffer from frequent episodes of acidosis caused by an increased fatty acid metabolism and consequently increased plasma level of acetoacetate (AcAc) and β-hydroxybutyrate (β-HOB). This article describes a study of the effects of pathological concentrations of AcAc and β-HOB on lipoperoxidation, cell viability and the release of the CXCL8 (IL-8) cytokine by activated neutrophils. Neutrophils from healthy donors were isolated by density gradient (Histopaque® 1077/1119) and incubated with the ketone bodies. Lipoperoxidation was determined as thiobarbituric acid reactive substances (TBARS). The cell viability was evaluated by the release of intracellular lactate dehydrogenase. The release of CXCL8 was measured by ELISA in a 24-h culture of opsonized zymosan-stimulated neutrophils. AcAc, but not β-HOB, provoked a dose-dependent increase in the neutrophil membrane lipoperoxidation (p<0.05; r =0.9915). In the cytotoxicity assay, a dose-dependent release of LDH was observed when the neutrophils were incubated with AcAc in concentrations up to 40 mM (p<0.05). β-HOB was devoid of effect. The release of CXCL8 was inhibited by AcAc and β-HOB in a dose-dependent manner. In conclusion, these results suggest that the accumulation of ketone bodies in diabetic patients could be involved in their usually increased susceptibility to infection.

Organização celular dos testículos em Hylidae e leptodactylidae, no Pantanal (Estado do Mato Grosso do Sul, Brasil)

The objective of this work was to make a comparative analysis of germ cell organization at different stages of cellular differentiation in adult males of Dendropsophus nanus (Boulenger, 1889), Pseudis limellum (Cope, 1862), P. paradoxa (Linnaeus, 1758), and Scinax acuminatus (Cope, 1862), belonging to the family Hylidae; and Leptodactylus chaquensis (Cei, 1950) and L. podicipinus (Cope, 1862), belonging to the family Leptodactylidae, collected in the Pantanal and in Serra da Bodoquena, Mato Grosso do Sul State, Brazil. The testes were removed and fixed, dehydrated in a graded series of ethanol and embedded in methacrylate glycol resin (Historesin Leica®). The sections were stained by 1% toluidine blue and observed under light microscope. It was detected that all individual of the Hylidae family show, throughout the year, the presence of all germ cell types of spermatogenesis. However, all Leptodactylidae family individuals only show the presence of all germ cell types during the rainy season. The variations of characteristics in seminiferous epithelium organization, as well as the evident difference in the amount of spermatozoa inside the tubules, are evidence that the anurans in this work show different forms of spermatogenesis development throughout the year: the cycle is continuous for the Hylidae family, and discontinuous with explosive release of spermatozoa for the Leptodactylidae family.

Impacto das tecnologias de informação e comunicação: cultura digital e mudanças sócio-culturais

The contemporaneous culture presents new con ditions of interaction with the information flows in the knowledge construction. Multidimensional codifications stimulate the brain and impress meaning to concepts initially empty. Culture is redefined as mosaic-culture, composed by a diversity of media and by immersion in the messages' sphere. In emerge nt countries like Brazil that propose to capacitate citizens for action and for interaction, the aims should imply the respect to the cultural specificities and in the real improvement of the citizens welfare. Information Science in this context must seek theorizations and practices that contribute to social improvements, capacitating and a better understanding of the challenges of Social Web. If the PISA examination demonstrates the County's frailness relating to the information, the 2011 Unicef report point to opportunities. The Universities have an essential role in the capacitating and in the creation of professional contingents, developing their own potentialities in the study, research and knowledge production for a better use of the Information and Communication Technologies. The man-machinery interfaces offer new problems to be dealt together with the literacy lags in Brazil: if information needs to be communicated to re-elaborate in knowledge, its representational supports need to be learnt as technological processes. Implementing informational ambiences studies will produce material for analyses, for the understanding and for the cultural practices importation, converting them to the contextualization of the Country's needs.

Atividade mutagênica e ativadora da resposta imune celular induzidas por Byrsonima crassa Niedenzu e Byrsonima intermedia A.Juss. (Malpighiaceae)

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Efeito da alimentação e densidade de estocagem no desempenho zootécnico e perfil celular do hepatopâncreas do camarão-da-amazônia Macrobrachium amazonicum (Heller, 1862) (Crustacea, Palaemonidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Poética da cabaça: fruto de tradição, arte e comunicação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mapeamento Mobile Art: propostas poéticas em telefones celulares : de 2001 a 2010

Pós-graduação em Artes - IA

MATIUDE - monitoramento e acesso a transdutores inteligentes (Padrão IEEE 1451) utilizando dispositivos embarcados (celulares e PDA´s)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da comunicação de um compensador série para rede de distribuição com o centro de controle utilizando tecnologia GPRS

Pós-graduação em Engenharia Elétrica - FEIS

Avaliação da suscetibilidade eletromagnética dos cabos metálicos dos sistemas de supervisão, proteção, comunicação e controle de subestações de alta tensão

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito α-tomatina na proliferação celular, apoptose e expressão de RNAm dos genes APC, Ciclina A2, Catenina, CASP9, BAK, BAX e BCL-XL em células HT29

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)