89 resultados para Caprino


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Zootecnia - FCAV

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Pós-graduação em Medicina Veterinária - FMVZ

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Pós-graduação em Zootecnia - FCAV

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Caprine arthritis-encephalitis (CAE) is routinely diagnosed with the Agarose Gel Immunodiffusion (AGID) technique, which is considered to have low sensitivity. The objective of this study was to standardize testing i-Elisa and Western Blot for early detection of antibodies against CAEV in goats and compare the results obtained in these tests with proof of AGID. For standardization of i-Elisa and WB, different concentrations and dilutions of antigen, sera and conjugate were used. In the i-Elisa, rigid microplate with 96 wells was adopted, and the combination that showed the best result was a concentration of 0.5µg/ well of antigen and dilutions of the serum of 1:100 and conjugate of 1:1500. In the WB nitrocellulose membranes were used, and the dilutions of the serum were defined at 1:50 and conjugate at 1:15000. To evaluate the performance of the techniques, 222 goat serum samples were tested and the data were compared with the AGID. The sensitivity and specificity of Elisa-i/IDGA, WB/AGID and WB/Elisa-i were 70% and 91%, 100% and 72.6%, 84.6% and 76.5%, concomitantly. The Kappa index of these tests was 0.35, 0.2 and 0.36, respectively. The i-Elisa and WB techniques were more sensitive than the AGID and can be used as tools for early diagnosis of CAE.

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The objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no complications were found during the laparoscopic procedures that would impair the reproductive future of the animals. Therefore, a viable number of oocytes were obtained even with the decreased reproductive efficiency, proving that repeated LOPUs do not interfere with the reproductive of adult and prepubertal nanny goats. These results indicate a positive aspect of this procedure, allowing for increasing reproductive performance of this kind, when used for the production in vitro.

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The arthritis-encephalitis caprine vírus is found at mostly areas of the world, especially at the countries that milk goats is strongly tecnific, and already was found in many brasilian states. The illness causes greats econimics prejudice, influencing badly the productive life time of the flock, related with the low production, decrease of the reproductive performance and lost of the genetic potential, determining constants flock’s renew. Many authors calls the atention for the probable dissemination of the illness and reinforce the necessity of the adoption strong sanitary politics for the illnes control. The controls programs, basically, based on knowledeg of the target-cells, that keep and distribute the virus on the guests organisms; on the transmition way; on sensibility and specificity of the sorologics tests, as well as your frequency of realitation in the flock; and after all on the management that the flock is submeted

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Pós-graduação em Medicina Veterinária - FCAV

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Pós-graduação em Medicina Veterinária - FCAV

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Pós-graduação em Medicina Veterinária - FCAV