Multiple cytogenetic clones in a basal cell carcinoma

Chromosome analysis of short-term culture of a basal cell carcinoma showed five clonal chromosome abnormalities, t(9;14)(q12 or q13;p11), del(1)(q23 or q25), trisomy 5, trisomy 7, and monosomy X. In addition, several nonclonal structural and numerical changes were seen in the tumor cells.

Estimativa do tamanho das fêmeas com ovos de Moina minuta Hansen, 1899 (Cladocera, Crustacea) no lago Amapá, Rio Branco, Estado do Acre Brasil

Moina minuta, a tropical species of Cladocera, was selected for the study of medium size at maturity and female egg production, due to its abundance and frequency in samples during low-water season in 1997 and high-water season in 1998, in Amapá Lake, Western Amazônia. The primiparous female had a size of 330 μm and 340 μm in low and high water, respectively. Moina individuals reached maximum size during high water (595μm). The medium size of the ovigerous females, in low water was 501 μm, producing an average of 4.41 eggs/female, while in high water, it was 533μm, with an average egg production of 4.84 eggs/female. The results, analyzed by the t test and Fischer's F test, showed that there were no statistical differences in size among individuals (t= -10.69, F=1.20 and P>0.1227), nor among ovigerous females (t= -4.45, F=1.02 and P>0.8906), considering the two seasons.

Drought tolerance of two field-grown clones of Coffea canephora

We compared tolerance to soil drought of two field-grown clones of Coffea canephora (clone 46, drought-sensitive; and clone 120, drought-tolerant). Under irrigation, there were no marked differences between the clones in water relation parameters, gas exchange and total leaf area. Under rainfed conditions, clone 46 showed osmotic adjustment and increased tissue rigidity. These adjustments, however, were incapable of preventing substantial decreases in xylem pressure potential. By contrast, clone 120 did not exhibit osmotic adjustment, but was able to increase tissue elasticity and to maintain xylem pressure potentials to a greater extent than clone 46 (despite having twice the total leaf area of this clone). Stomatal conductance was lowered by drought in clone 120 but not in clone 46. Carbon assimilation per unit leaf area in both clones remained unaffected under stress. Long-term water use efficiency (WUE), as estimated through carbon isotope discrimination, was consistently greater in clone 120 than in clone 46. Because of these traits, clone 120 was better able to postpone dehydration and to maintain whole-tree photosynthesis. It is proposed that these features should decisively contribute to buffer its productivity in drought-prone areas. © 2002 Elsevier Science Ireland Ltd. All rights reserved.

Thermogravimetric studies of the decomposition kinetics of four different Hevea rubber clones using Ozawa's approach

Thermal degradation of natural rubber extracted from four different rubber clones of the Hevea brasiliensis species was investigated by thermogravimetry using Ozawa's approach to assess the kinetic parameters of the decomposition process. The results are discussed in terms of the order of reaction, kinetic parameters such as activation energy as a function of conversion degree, and thermal stability for each of the clones.

Parâmetros genéticos para caracteres de crescimento, de produção e tecnológicos da madeira em clones de Eucalyptus spp

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mapeamento da produtividade e perímetro do tronco de clones de seringueira em argissolo vermelho amarelo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Genetic divergence in Eucalyptus spp. clones by molecular RAPD markers

The genetic divergence in 20 Eucalyptus spp. clones was evaluated by multivariate techniques based on 167 RAPD markers, of which 155 were polymorphic and 12 monomorphic. The measures of genetic distances were obtained by the arithmetic complement of the coefficients of Jaccard and of Sorenso-Nei and Li and evaluated by the hierarchical methods of Single Linkage clustering and Unweighted Pair Group Method with Arithmetic Mean (UPGMA). Independent of the dissimilarity coefficient, the greatest divergence was found between clones 7 and 17 and the smallest between the clones 11 and 14. Clone clustering was little influenced by the applied procedure so that, adopting the same percentage of divergence, the UPGMA identified two groups less for the coefficient of Sorenso-Nei and Li. The clones evidenced considerable genetic divergence, which is partly associated to the origin of the study material. The clusters formed by the UPGMA clustering algorithm associated to the arithmetic complement of Jaccard were most consistent.

Comportamento fisiológico e morfológico de clones de Eucalyptus urograndis submetidos a diferentes níveis de água no solo

The present study had as its objective the assessment of the possible effects of hydric stress on the growth, physiological characteristics of two different genetic materials from Eucalyptus urograndis. The experiment was carried out in a greenhouse at Faculdade de Ciências Agronômicas of UNESP, campus Botucatu from March to July, 2005. The hydric management was established based on the soil water potential. Two water levels were established, doing the evapotranspired water replacement by pot weighing. Two clones were used, Eucalyptus urograndis 105 and 433, being the first one more resistant to the hydric deficit and the 433 more sensitive to stress. The study was made from a 2×2 factorial (two levels of water × two genetic materials). For the hydric management, the plants were irrigated when they reached a soil water potential of -0.03 MPa or -1.5 MPa. The assessments made were: diffusive water vapor of stomato, transpiration, leaf temperature and leaf water potential. The physiological evaluations throughout the day, in the end of the experiment. Treatments without hydric stress had a higher performance in all studied characteristics, but the clones had no influence. The stomatic resistance followed the potentials, showing higher values in the treatments submitted to hydric deficiency, more intensely for clone 433, being that this also happened with the leaf water potential. The transpiration also followed the leaf water potential and the stomatic resistance more intensely for clone 105 both comparing stressed plants and non-stressed plants. Consequently, the leaf temperatures had higher values for clone 433 on the stressed treatment. Thus, it can be concluded that there was a better performance in plants kept on a soil water potential of -0.03 MPa and a higher resistance to hydric stress for clone 105.

Geologic e geoquímica do batólito Rapakivi Rio Branco, SW do Craton Amazônico - MT

The Rio Branco Rapakivi Batholith belongs to the Cachoeirinha Tectonic Domain, part of the Rio Negro-Juruena Geochronological Province located on the southwestern portion of the Amazonian Craton in Mato Grosso, Central Brasil. A systematic geological mapping on a 1:100.000 scale, coupled with petrographic and geochemical studies allowed to redefine this batholithic unit, to recognize faciological variations and to characterize the geochemical features of this rapakivi magmatism. The batholith is constituted by two major plutonic suites, the first forming a basic suite of fine-grained, equigranular, mesoto melanocratic gray to black lithotypes, with usually discontinuous porphyritic varieties located near the margins of the intrusion. The second one is characterized by acid to intermediate rocks constituted by porphyritic granites, in part granophyric, with rapakivi textures. They have K-feldspar phenocrysts of up to 4cm. Three distinct petrographic facies are recognized in this suite: 1. equigranular to pegmatitic monzogranites; 2. red rapakivi leuco-monzogranites; 3. dark red rapakivi monzogranites to quartz-monzonites. Rocks present SiO2 contents from 67% to 73%, show peraluminous to metaluminous compositions and define a high-K calc-alkaline to shoshonitic magmatism in an I- and A-type, post-orogenic to anorogenic intraplate environment. The magmatic processes are associated with the end of the collisional event that consolidated and stabilized the SW part of the Amazonian Craton.

Eficiência de uso de boro no crescimento de clones de eucalipto em vasos

This paper evaluated the critical level, responsivity and boron use effciency on growth and the biomass production in six Eucalyptus grandis x Eucalyptus urophylla clones (Ca, Cb, Cc, Cd, Ce and Cf). An experiment was carried out in pots containing ground silicon, under greenhouse conditions using four boron rates per solution (0; 0.135; 0.27; 0.54 mg L -1 of B). The treatments were combined in a randomized block experimental design in a 4 × 6 factorial scheme with three replications. Plant height, stem diameter, dry matter production in the shoots of the plant and boron use effciency in the leaves, stem and total dry matter at the 8th month of age were evaluated. Due to boron fertilization, growth and shoot biomass were observed in the Eucalyptus plants after 240 days of being planted in pots. The increases in growth and biomass were 35 to 54% and 21 to 64%, respectively. The boron rates that promoted major growth of the plants were 0.33 to 0.44 mg L -1 of B and in this range the most effcient clone for dry matter production of leaves was Cf and the least effcient one to stem biomass production and the shoot biomass was Cd, no signifcant differences among other clones were observed. The critical level of boron in solution was 0.09 to 0.24 mg L -1 of B in the growth of the plants.

Ocorrência do peixe-pescador-branco, Lophiodes beroe Caruso, 1981 (Lophiiformes: Lophiidae), em águas brasileiras

One specimen of the lophiid white anglerfish, Lophiodes beroe was collected for the second time in Brazilian waters, which far extends the limit distribution of the species to southeastern region (25°00'08).

Análise de crescimento em clones de eucalipto submetidos a estresse hídrico

The aim of this study was to evaluate Eucaliptus grandis genotypes (Clones 105 and 433) in relation to drought tolerance, through growth plant analysis. Black PVC pots with 10 liter volume were used for cultivate plants in polyethilene greenhouse oriented east/west. Completely randonmized design with four treatments was used: two clones and two minimum soil water potentials (- 0.03 and -1,5 MPa) and sixteen replicates. Pots were weighed daily in order to evaluate water content and characteristic soli water curve was determined. Plant development was obtained each 15 days from planting until 60 days through total dry matter (DM), leaf area index (LAI), leaf area ratio (LAR), net assimilative ratio (NAR), specific leaf area (SLA), relative growth ratio (RGR) and absolute growth ratio (AGR). Results showed that clone 105 presented less sensibility to water deficit, which qualify it as genetic material for use under dry soil conditons. On the other hand, both clones had similar behavior with no water restrictions.

Respostas de clones de Eucalyptus grandis e E. grandis x E. urophylla à supressão de boro

The present work had as objective the study of clones of Eucalyptus grandis and E. grandis × E. urophylla under boron suppression on growth in height and diameter, development of symptoms of deficiency, boron content in leaf and polyols production. Plants were cultivated in pots with quartz in a greenhouse using two levels of boron per solution (0 and 0.5 mg L-1 of B). The 32 treatments followed a factorial scheme: 16 × 2 -16 clones and two doses of boron in a randomized block experimental design, with fve repetitions, totaling 160 plots. Plants were evaluated weekly for deficiency symptoms and monthly for height, stem diameter and leaf content of boron in different plant parts. The content of polyols was measured in two occasions: at 162 days and 192 days after starting the experiment. Decrease of growth and quick development of deficiency symptoms 40 days after boron suppression were observed, as well as a decrease of polyols synthesis. Foliar analysis, with boron supplement, resulted in the presence of mannitol and sorbitol in high enough concentration so that the evaluated plants could be considered rich in those polyols. Under boron suppression, boron levels in different portions of branches and stems of plants, as well as the presence of mannitol and sorbitol, indicated a conditional mobility of boron in Eucalyptus.

Fungigação no controle do mofo branco e produtividade do feijoeiro em condições de cerrado brasileiro

The aim of this research was to evaluate the white mold severity (Sclerotinia sclerotiorum (Lib.) of Bary), bean production components and yield (Phaseolus vulgaris L.), variety Perola, according to the application of procimidone fungicide (Sialex 500), through fungigation (center pivot) and automotive sprayer (Uniport). The study was carried under field production commercial conditions, in Primavera do Leste - MT - Brazil. The experiment consisted of 5 treatments (with 4 repetitions of 4 ha each), all with two procimidone applications (1.2 kg ha-1 each application, same as, 0.6 kg a.i. per hectare) to the 42 and 52 days after seeding. The water depths of 5.5 and 11.0 mm were tested in the application through central pivot (this had your checked uniformity), providing volumes of 55.000 and 110.000 L ha-1, respectively, and the volumes of 120 and 200 L ha-1 in the automotive sprayer. The severity of disease was evaluated by the percentage of the area affected by plant damage using diagramatic grade scale of white mold severity, as described by Azevedo (1998). The values were used to calculate the area under the disease progress curve (AUDPC). They were also analyzed, the number of the fungus apothecia during the crop cycle and the residual sclerotias weight in harvest. On this occasion, it was also evaluated the crop yield parameters: number of plants per plot (final stand), pods per plant, grains per pod, medium weight of 200 grains and productivity of grains. The AUDPC values, apothecia to 42, 49 and 56 days after seeding, sclerotias in 2 soil kg and the crop productivity parameters were submitted to the variance analysis and Tukey Test at 0.05 of probability. This test was also applied in the comparison among the different fungicide application methods, independent of spray volumes in each one. The statistical processing was accomplished by STAT program. The results showed that weren't differences among application techniques studied in relation to productivity parameters, however, best white mold control, smaller apothecia number to 49 and 56 days after seeding and smaller weight of residual sclerotias in the harvest were obtained with the fungigation, independently of the spray volume used.

Capim-colonião e seus efeitos sobre o crescimento inicial de clones de eucalyptus × urograndis

This study aimed to evaluate the guinea grass effect (Panicum maximum) on the initial growth of different Eucalyptus × urograndis clones. Two assays were established with eucalyptus clones and guinea grass seedlings. The plants were grown in plots with cement borders filled with soil. Each plot received a eucalyptus seedling. The first assay had a completely randomized experimental design, with three replications, and treatments in a 5x2 factorial scheme (five eucalyptus clones and the absence or presence of two guinea grass plants at 10 cm distance from eucalyptus seedling). The second assay was similar to the first, however with three eucalyptus clones. The experimental design was completely randomized, with five replications, and a 3x2 factorial scheme (three eucalyptus clones and the absence or presence of two guinea grass plants). The presence of eucalyptus clones did not affect guinea grass development. The eucalyptus clones that coexisted with guinea grass plants did not show differences in their development, making the clones equal when under competition. The most susceptible characteristics of eucalyptus clones to guinea grass were foliar area, shoot and stem dry matter. Clone 3 showed the most sensitivity to guinea grass, and clone 1 was the most tolerant, but all clones studied suffered a negative interference from guinea grass.