Purification, sequencing and structural characterization of the phospholipase A(1) from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)

The biochemical and functional characterization of wasp venom toxins is an important prerequisite for the development of new tools both for the therapy of the toxic reactions due to envenomation caused by multiple stinging accidents and also for the diagnosis and therapy of allergic reactions caused by this type of venom. PLA(1) was purified from the venom of the neotropical social wasp Polybia paulista by using molecular exclusion and cation exchange chromatographies; its amino acid sequence was determined by using automated Edman degradation and compared to the sequences of other vespid venom PLA(1)'s. The enzyme exists as a 33,961.40 da protein, which was identified as a lipase of the GX class, liprotein lipase superfamily, pancreatic lipases (ab20.3) homologous family and RP2 sub-group of phospholipase. P. paulista PLA(1) is 53-82% identical to the phospholipases from wasp species from Northern Hemisphere. The use restrained-based modeling permitted to describe the 3-D structure of the enzyme, revealing that its molecule presents 23% alpha-helix, 28% beta-sheet and 49% coil. The protein structure has the alpha/beta fold common to many lipases; the core consists of a tightly packed beta-sheet constituted of six-stranded parallel and one anti-parallel beta-strand, surrounded by four alpha-helices. P. paulista PLA(1) exhibits direct hemolytic action against washed red blood cells with activity similar to the Cobra cardiotoxin from Naja naja atra. In addition to this, PLA(1) was immunoreactive to specific IgE from the sera of P. paulista-sensitive patients. (c) 2007 Elsevier Ltd. All rights reserved.

The effects of the social hierarchy destabilization on the foraging activity of eusocial wasp Mischocyttarus cerberus styx Richards, 1940 (Hymenoptera: Vespidae: Polistinae)

The genus Mischocyttarus comprises 245 species of neotropical basal eusocial wasps. They form small colonies (rarely more than few tens of individuals); castes are morphologically undifferentiated and determined behaviorally by agonistic interactions. The aim of this study was to verify the effects of the experimental disruption of social hierarchy on foraging activity of Mischocyttarus cerberus styx. We observed six colonies in postemergence phase and recorded data on the foraging activity under two experimental conditions: (1) removal of lower-ranked females and (2) removal of higher ranked females, except the queen. Our results showed that the removal of higher-ranked females had higher effect on the number of foraging trips of M. cerberus styx than the removal of lower-ranked females (the number of foraging trips/hour decreased by 66.4 and 32.7, resp.). Such results are likely due to the social organization of this species and the presence of a distinct class of females, which in this study were regarded as intermediates. Our data also showed that, irrespective of the hierarchical status of the females, the removal of two or three individuals affected significantly the number of foraging trips in this species. Copyright 2011 Vanderlei Conceio Costa Filho et al.

Orquestra Sinfônica de Ribeirão Preto (SP): representações e significado social

Pós-graduação em Música - IA

Estudo intergeracional com indivíduos em condição de vulnerabilidade social: concepções de família

Pós-graduação em Psicologia do Desenvolvimento e Aprendizagem - FC

Participação, controle e Serviço Social na política municipal de assistência social de Uberaba/MG: tensão entre a radicalização democrática e as estratégias de reprodução do capital

Pós-graduação em Serviço Social - FCHS

A interlocução do Serviço Social com os movimentos sociais no Brasil: uma análise a partir da produção teórica com enfoque no trabalho profissional

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O impacto social da hemodiálise para classe trabalhadora

Pós-graduação em Serviço Social - FCHS

Discurso e classe: mobilidade social refletida no discurso do empresariado calçadista de Franca - SP

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A insustentável teoria da sustentabilidade: Ideologia e reificação no discurso empresarial da responsabilidade social no Brasil

Pós-graduação em Ciências Sociais - FFC

A sociabilidade do capital e o gerenciamento da questao social: o projeto ético-político profissional no cotidiano do assistente social

Pós-graduação em Serviço Social - FCHS

O uso social da leitura e as práticas pedagógicas em sala de aula: construção de conhecimentos e formação de leitores

This research aimed at studying the social role of reading, its importance in building knowledge and training of readers, as well as reflect on the teaching of reading in schools, with children in the literacy process. For their achievement, were used as theoretical support authors who are dedicated to the study of language, thematic reading, and phenomena that occur through it in the school environment, especially those of Bakhtin, Kleiman, Chartier, Foucambert, among others . The research was carried out using the approach of qualitative research, using participatory action research, through which the researcher could have direct contact with the observed phenomena, to participate and collect the participants' actions in its natural context, the from their perspective and their views. As for collecting and analyzing data, we used the tools of questionnaire, interview and participant observation. Its subjects a literacy class and their respective teacher, in 2009. By analyzing this information, one can draw a picture of reading in the school environment and teaching practices that surround this object. The results of the literature survey and data analysis suggest that reading is a social practice, and as such has indispensable social function in society today. And the school, one of the greatest instruments of contact with the world of letters, therefore, of reading for children means not literate, have key role in developing and training of readers who are aware of the importance of reading and perpetuate this practice in their daily lives. Similarly, subjects of research, have achieved an advanced level of literacy and understand the social role of reading and its importance to live in society, in which they belong

A apropriação das obras de Ricardo Antunes pelo Serviço Social: a categoria trabalho em debate

Pós-graduação em Serviço Social - FCHS

Melodrama em falso e pedagogia social de Capitu, de Luiz Fernando Carvalho: a adaptação de Dom Casmurro para o audiovisual em três lições

Some esthetical particularities of Luiz Fernando Carvalho’s micro-series reveal, in the nineteenth century Brazil, the capacity of the literary and audiovisual discourse to represent some singularities of the Brazilian social life from the past, as well as pointing to existential problems of the class representative that leads the plot, instigating reflections in the present. The treatment offered to the class struggle by the Machadian narrative and by the Rede Globo’s adaptation, just as the way in which the existential drama is expounded, disclose a kind of reception among readers/viewers which is capable of reviewing the historical past in times that ask for such a revision, even though the television series bets on the non-conventional. Machado presented the tension between representation and truth (as well as between emotion production and reflection) with the transfiguration of the tragic pathos and the mobilization of the patriarchal figure to the melodramatic bias. Although increased by the irony orchestrated by the scenic elements, in the audiovisual version such Machadian solutions would be apt to lose their strength to unmask the social subjects; on the other side, the intensification of the melodrama can make evident what Machado disguised with less obvious narrative intentions.

Multidisciplinary treatment ofadult patients with Class III malocclusion

This article describes the multidisciplinary treatment of an adult patient presenting with Angle Class III malocclusion, alteration of the mandibular position, vertical alveolar bone loss and absence of teeth in the lower posterior region. With advancing age the existence of occlusal interference due to loss of teeth or tooth structure is very common, resulting in periodontal problems due to occlusal trauma. The options for treatment of Class III malocclusion in adolescent and adult patients include compensatory orthodontic treatment in mild to moderate cases and orthognathic surgery for moderate to severe cases. The combination of various dental specialties enabled improvement in the social circumstances of the patient. This can be observed objectively by the final dental relationship and by the skeletal and tegumentary cephalometric comparison between the situation at the beginning and at the end of the treatment. The compensatory treatment performed permitted the successful correction of a Class III malocclusion in the clinical case presented.

Fazer ciência, fazer história: a sociologia da mudança social de Florestan Fernandes e de Costa Pinto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)