Imunidade celular em caninos neonatos - do nascimento ao 45° dia de idade

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A utilização de glicerina em dietas para bovinos altera a microbiota ruminal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Inflammatory response of endothelial cells to hepatitis C virus recombinant envelope glycoprotein 2 protein exposure

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência do estímulo verbal no tempo do teste de escada

Introduction: The stair-climbing test (SCT) is considered a submaximal test of simple implementation and easy access, which assesses the individual’s functional capacity. Although widely used in surgical patients, there is no standardization regarding the height of the stair and verbal stimulus. Objectives: It was determine if verbal stimulus changes the stair-climbing time (SCt) in individuals over 50 years-old. We compared oxygenation, blood pressure, pulse, respiratory rate and Borg scale between SCT performed with and without stimulation. Methods: We evaluated individuals with ages greater than 50 years-old that performed two STC (with and without verbal stimulation), in the stair with 44 steps, achieving 7.04 m in height and recording the time to climb the stair. The systolic blood pressure (SBP) and the diastolic blood pressure (DBP), respiratory rate (RR), pulse, oxygen saturation (SpO2 ) and Borg scale were evaluated before and after the tests. The time in the SCT with and without stimulation were compared using the Student test-t and the other variables were compared using the ANOVA test (p<0.05). Results: The average age was 59.75±6,40 years old in the 21 evaluated individuals. The time in the SCT without stimulus was significantly higher than the one with stimulus. The SBP, pulse, RR and Borg scale were significantly increased when compared to the SCT with and without stimulus. Oxygenation and DBP didn´t have significant differences at any time of the study. Conclusions: The time in the SCT was lower when performed with verbal stimulus. The SBP, pulse, RR and Borg scale changed significantly after the SCT with and without stimulus, remarking that this change was greater in the SCT with stimulus.

Determination of equine fetal sex by Doppler ultrasonography of the gonads

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Seleção genômica para características de carcaça em bovinos da raça Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV

Eficiência do aparelho Herbst bandado na correção da Classe II divisão 1 -relato de caso

The current reports the case of a young patient with malocclusion Class II division 1 on permanent dentition treated in two stages, rthopedics and orthodontics, respectively. At first, the banded Herbst appliance was used duringa 7 months period, followed by a T4K appliance, Trainer for Kids used as retention, and on the second stage the corrective Orthodontics was performed. The results showed the acquirement of a Class I dental relationship, which was kept stable, with excellent intercuspation, even after 5 years of the removal of the Herbst appliance, as well as the correction of the overjet and the reduction of the facial profile convexity. It can be concluded that the Herbst appliance was very efficient in correcting the Class II malocclusion, long term, also providing a very favorable effect on the facial profile.

Effect of hydrogen-peroxide-mediated oxidative stress on human dental pulp cells

To evaluate the effect of the oxidative stress on human dental pulp cells (HDPCs) promoted by toxic concentrations of hydrogen peroxide (H2O2) on its odontoblastic differentiation capability through time. Methods HDPCs were exposed to two different concentrations of H2O2 (0.1 and 0.3 μg/ml) for 30 min. Thereafter, cell viability (MTT assay) and oxidative stress generation (H2DCFDA fluorescence assay) were immediately evaluated. Data were compared with those for alkaline phosphatase (ALP) activity (thymolphthalein assay) and mineralized nodule deposition (alizarin red) by HDPCs cultured for 7 days in osteogenic medium. Results A significant reduction in cell viability and oxidative stress generation occurred in the H2O2-treated cells when compared with negative controls (no treatment), in a concentration-dependent fashion. Seven days after H2O2 treatment, the cells showed significant reduction in ALP activity compared with negative control and no mineralized nodule deposition. Conclusion Both concentrations of H2O2 were toxic to the cells, causing intense cellular oxidative stress, which interfered with the odontogenic differentiation capability of the HDPCs. Clinical significance The intense oxidative stress on HDPCs mediated by H2O2 at toxic concentrations promotes intense reduction on odontoblastic differentiation capability in a 7-day evaluation period, which may alter the initial pulp healing capability in the in vivo situation.

Comportamento biomecânico dos sistemas de retenção de overdentures

Objective: to the purpose of this study was to compare the stress distribution in the peri-implant hard tissue on different attachment systems for mandibular or maxillary implant-retained overdentures. Material and methods: the search for published studies was performed on PubMed/Medline database covering the period of January 2000 to April 2014. The selection of the eligible studies was performed according to including and excluding criteria. Results: a total of 140 studies were screened and according to the search strategy, 21 studies were selected for this review. Eight studies perfomed strain-gauge analysis, 5 evaluated the stress distribution though photoelastic test and 7 performed tridimensional finite element analysis. Only one study in vivo was included. Non-splinted O-rings showed better stress distribution than other bar-clip attachment systems. Conclusions: the present study did not find sufficient evidences regarding the most indicated attachment system for overdentures with better stress distribution for the peri-implant hard tissue. The methodologies analyzed should be complemented with other tests and used as a tool for further clinical studies.

Prospecção de assinaturas de seleção em regiões de QTL associadas com características reprodutivas em novilhas Nelore

Pós-graduação em Genética e Melhoramento Animal - FCAV