Validation of Relapse Risk Biomarkers for Routine Use in Patients With Juvenile Idiopathic Arthritis

Objective. The myeloid-related proteins 8 and 14 (MRP-8/MRP-14) and neutrophil-derived S100A12 are biomarkers of inflammation. They can be used to determine the relapse risk in patients with juvenile idiopathic arthritis (JIA) after stopping antiinflammatory treatment. In this study, we tested the performance of different enzyme-linked immunosorbent assays (ELISAs) in order to validate systems available for routine use.Methods. MRP-8/MRP-14 and S100A12 serum concentrations of 188 JIA patients in remission were analyzed. Commercially available test systems were compared to experimental ELISAs established in house. The ability of the assays to identify JIA patients at risk for relapse was analyzed.Results. For MRP-8/MRP-14, the PhiCal Calprotectin and Buhlmann MRP8/14 Calprotectin ELISAs revealed hazard ratios of 2.3 and 2.1, respectively. For S100A12, the CircuLex S100A12/EN-RAGE ELISA revealed a hazard ratio of 3.1. The commercial assays allowed a JIA relapse prediction that was at least comparable to the experimental ELISAs.Conclusion. For the prediction of JIA relapse after stopping medication, the biomarkers MRP-8/MRP-14 and S100A12 can be determined by using assays that are available for routine use. The tested commercial MRP-8/MRP-14 and S100A12 ELISAs showed a performance comparable to well-established experimental ELISA protocols when assay-specific cutoffs for the indication of relapse prediction are thoroughly applied.

Comparison surface characteristics and chemical composition of conventional metallic and Nickel-Free brackets

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

La interculturalidad en la enseñanza del español como lengua extranjera en la pasantía de la práctica profesional

In the contemporary literature on Foreign Language Teaching (LE/L2), is evidence of a trend in education given priority from the sub-focus on socio-cultural competence (HYMES, 1972), mainly with the aim of developing the intercultural competence, that is, the power to know the differences between the culture of the new language and the language in concrete situations and to develop strategies to deal comprehensively with the customs of another culture (Rozenfeld & Vianna, 2011). This process seeks to bring people to a critical analysis of their own language and culture, the concept of otherness and the similarities and differences between nations (MOTTA ROTH, 2003). Under the didactic-pedagogical approach multiculturalism, there is the need to develop new educational proposals and to include studies and researches about this subject in the curricula of teacher education courses. Politically, the proposals of the "Parâmetros Curriculares Nacionais (PCNs)" in Brazil emphasize as targets to be established for the teaching of Spanish as a foreign language (ELE), on average, target content and teaching strategies to develop, in addition to communicative competence, competition also "inter (multi) culture" (BRASIL, 2000). Thus, this paper analyzes the reports of pre-service teachers in the course of Teaching Practice of Spanish Language, the presence of cultural and intercultural approach, verifying didactic-pedagogical treatment that is being expressed in projects and materialized in practices of ELE classroom, in the interior of São Paulo / Brazil.

La interculturalidad en la enseñanza de español como lengua extranjera en la pasantía

In the contemporary literature on Foreign Language Teaching (LE/L2), is evidence of a trend in education given priority from the sub-focus on socio-cultural competence (HYMES, 1972), mainly with the aim of developing the intercultural competence, that is, the power to know the differences between the culture of the new language and the language in concrete situations and to develop strategies to deal comprehensively with the customs of another culture (Rozenfeld & Vianna, 2011). This process seeks to bring people to a critical analysis of their own language and culture, the concept of otherness and the similarities and differences between nations (MOTTA ROTH, 2003). Under the didactic-pedagogical approach multiculturalism, there is the need to develop new educational proposals and to include studies and researches about this subject in the curricula of teacher education courses. Politically, the proposals of the "Parâmetros Curriculares Nacionais (PCNs)" in Brazil emphasize as targets to be established for the teaching of Spanish as a foreign language (ELE), on average, target content and teaching strategies to develop, in addition to communicative competence, competition also "inter (multi) culture" (BRASIL, 2000). Thus, this paper analyzes the reports of pre-service teachers in the course of Teaching Practice of Spanish Language, the presence of cultural and intercultural approach, verifying didactic-pedagogical treatment that is being expressed in projects and materialized in practices of ELE classroom, in the interior of São Paulo / Brazil.

Algas verdes coloniais em riachos de Unidades de Conservação do Sul do Brasil: taxonomia e aspectos ecológicos

Species of colonial green algae from 10 Conservation Units from the Southern Brazil were surveyed. The samplings were carried out in 105 stream reaches, consisting of 10 m length transects. In addition to biological data, some environmental variables were measured: depth, current velocity, water temperature, turbidity, specific conductance, pH, dissolved oxygen and nutrients (total nitrogen and orthophosphate). Three species of colonial green algae [Bohlin Ecballocystis pulvinata var. pulvinata, Tetraspora gelatinosa (Vaucher) Desvaux and T. lubrica (Roth) C Agard] were recorded and these species were described and illustrated. Based on the environmental data, it was observed some patterns of ecological distribution, including the occurrence of E. pulvinata var. pulvinata in environments with higher water temperature and current velocity values, while Tetraspora species typically occurred in environments with lower total nitrogen, orthophosphate and specific conductance values.

Flow sorting and exome sequencing reveal the oncogenome of primary Hodgkin and Reed-Sternberg cells

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Analysis of mesiodistal angulations of preadjusted brackets

Manufacturers offer various prescriptions of preadjusted brackets for use in the "straight-wire" orthodontic technique. However, the need to incorporate bends in the rectangular wires during orthodontic finishing has led to concerns regarding the type of prescription chosen and the credibility of information provided by the manufacturer. The aim of this study was to compare the slot angulations of Roth prescription preadjusted metallic brackets for the maxillary left central incisor and maxillary left canine. For each tooth type, 10 brackets of three commercial brands (GAC, Forestadent and Morelli) were selected. Two individual metal matrices for brackets and tooth positioning were made for each group of teeth. Captured images were obtained by standardized ortho-radial photography with a digital camera. Images were exported and analyzed with the Image J software package. One-way ANOVA and Tukey statistical analyses were performed at the 5% significance level. For brackets of the maxillary left central incisor, differences in mean angulation were observed between the Morelli and GAC groups (p < 0.01) and between the Forestadent and GAC groups (p < 0.01). For brackets of the maxillary left canine, differences in mean angulation were found between the Morelli and GAC groups (p < 0.01) and between the Morelli and Forestadent groups (p < 0.05). In conclusion, despite their same prescription name, the different brands exhibited significantly different angulation measurements.

Avaliação dos preparos da superfície cerâmica para a colagem de acessórios ortodônticos

The purpose of this study was to evaluate the effects of two conditioning methods used to enhance the shear strength of orthodontic brackets bonded to porcelain surfaces. A total of 18 feldspathic specimens were used. The specimens were divided randomly into two groups (n = 9): group free silane, the porcelain specimens were etched with hidrofuoric acid 10% (Acid Gel-Maquira) for 4 minutes followed by adhesive-primer (Transbond XT) and the metallic brackets (Morelli Roth Light .022" x .030") were bonded with a light-cured microfilled resin (Transbond XT Light Cure Orthodontic Adhesive); group silane, the porcelain specimens were etched with hidrofuoric acid 10% (Acid Gel-Maquira) for 4 minutes followed by silane (Silano Ângelus) for 1 minute, adhesive-primer (Transbond XT) and the metallic brackets (Morelli Roth Light .022" x .030") were bonded with a light-cured microfilled resin (Transbond XT Light Cure Orthodontic Adhesive). All specimens were stored in solution of artificial saliva at ambient temperature for 24 hours. The debonding was done with shear strength through a universal testing machine (DL 500-Emic) calibrated with a fixed speed of 1mm/minute. Statical analysis was performed using the Student t test. The results indicated that in the free silane group the mean bond strength was 9,97 MPA, significantly lower than the silane group, that was 12,38 MPa (p < 0,05). The both groups were effective for bonding, although the silane group had the highest bond strength values.