Eficiência do controle de nematóides, ferrugem e bicho mineiro em cafeeiros

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Patogenicidade de Meloidogyne incognita e Meloidogyne javanica a bananeira cv. Prata Anã em diferentes substratos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Espécies de Meloidogyne Goeldi em cafeeiro no município de Araguari-MG

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Reação de genótipos e híbridos de tomateiro à Meloidogyne enterolobii

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Reação de maracujazeiro amarelo a Meloidogyne Incognita Raça 3

This study concerned the reaction of yellow passion fruit ‘Maguary’ and ‘Afruvec’ to the phytonematode Meloidogyne incognita race 3 in greenhouse conditions. An entirely randomized experimental design with 3 treatments (‘Maguary’, ‘Afruvec’, and tomato cv. ‘Rutgers’) and 4 repetitions was used, each plot consisting of 1 vase containing 1 plant. After 6 months, an evaluation was made of the index of galls and egg mass in the yellow passion fruit varieties and in the tomato cv. ‘Rutgers’. The classification of resistance to the phytonematode was made by criterion of the reproduction factor (RF). ‘Maguary’ presented a zero index of galls and egg mass, while ‘Afruvec’ showed a low index of galls and egg mass in relation to the tomato cv. Rutgers. According to the RF, ‘Maguary’ was characterized as immune to the phytonematode, while ‘Afruvec’ was resistant, and the tomato cv. ‘Rutgers’ was susceptible.

Susceptibility of winter crops to Pratylenchus brachyurus and effect on the nematode population in the maize crop

The aim of this study was to evaluate the susceptibility of winter crops to Pratylenchus brachyurus and their effect on the population of phytonematodes in the maize. To study the effect of the plants on nematodes, an experiment was set up in sandy, naturally-infested soil. The area was divided into strips, consisting of six blocks of 16 treatments, with eight winter treatments, subdivided on the basis of the fertilizer used (organic: bird litter, and synthetic: NPK). The initial nematode population was determined by sampling the soil (100 cm(3)) and weeds (10 g of root). The winter treatments put in place (bristle oats, chickpea, vetch bean, common bean, oilseed radish, wheat, intercropped bristle oats + oilseed radish and fallow), and the nematode population determined 100 days after sowing. Subsequently, two maize crops (summer and short season) were planted, and the nematode population in the soil and roots determined during crop full bloom. To evaluate the susceptibility of winter crops to nematodes, an experiment was conducted under controlled conditions, determining the nematode reproduction factor (RF) in the treatments described above. Both in the field and under controlled conditions, it was observed that the bristle oats, oilseed radish and intercropped oats + oilseed radish exhibited lower reproduction rates for P. brachyurus. In the field, lower population of nematodes was observed with the application of bird litter. Under controlled conditions, the highest RF were observed in the fallow plot and under common bean and chickpea, in that order.

Resistance of rose rootstocks to Meloidogyne hapla nematode

Roses are widely used in landscaping. One of the most important fitossanitary problems of this ornamental plant in gardens is the fitonematodes, especially Meloidogyne hapla, which is the most important one in colder climate regions. This work had the objective of study the resistance of nine rose rootstocks (Rosa multiflora 'Paulista', R. multiflora 'Japanese', R. multiflora 'Iowa', R. multiflora 'Kopman's', R. indica × multiflora, R. indica 'Mayor', R. sp. 'Natual Brier', R. manetti and R. canina 'Inermis') to Meloidogyne hapla nematode. Ten replications of each rootstock were used. The roots, collected in a commercial area of cut roses, visually had galls there were isolated and identified. The inoculum of M. hapla was previously produced in rose and tomatoes seedlings under greenhouse conditions. The plants were harvested ninety days after inoculation. The roots were washed and the number of eggs and juveniles recuperated in the root system of each plant was estimated by the Final Population (FP), and the Reproductive Factor (RF) was calculated. The rootstocks with RF<1 values were considered resistant, and the ones with RF>1, susceptible. Rosa manetti and Rosa sp. 'Natual Brier' rootstocks showed the lowest values for RF, indicating that, although they are susceptible, they provide the lowest nematode reproduction. Based on the RF values, it was concluded that all the rose rootstocks evaluated were susceptible to M. hapla nematode.

Aggressiveness of pratylenchus brachyurus to sugarcane, compared with key nematode p. zeae

Pratylenchus zeae, Meloidogyne javanica and M. incognita are considered key species of nematodes in sugarcane in Brazil, but P. brachyurus is also frequently found. This study was conducted to determine the aggressiveness of P. brachyurus compared with P. zeae to sugarcane. Plants were grown in pots (100 L) in an open area with initial inoculation of 10, 100, 1,000, 10,000 and 100,000/plant for P. brachyurus and P. zeae. The nematode inocula were from in vitro, carrot-cylinder cultures. Sampling was performed every 60 days until 300 days after inoculation. At harvest, we evaluated the population dynamics of the nematodes and plant growth characteristics. The population for the initial levels of 10 and 100,000 specimens/plant, for P. brachyurus and P. zeae at 300 days after inoculation were similar. This fact shows that, upon detection of nematodes in a certain place during the planting of sugarcane, the ratoon on this area should be treated so as to control populations of P. brachyurus and/or P. zeae. The damage caused by the initial population of 10 specimens of P. brachyurus was similar to those of 10.000 specimens of P. zeae. The variety CTC 2 was classified as susceptible to P. zeae and intolerant to P. brachyurus. Compared to the control, the losses as measured by the volume and fresh weight of shoots by the nematode species were 29.82% and 40.34%, respectively. Pratylenchus brachyurus was more aggressive than P. zeae to the CTC 2 sugarcane variety.

Anthelmintic efficacy of oral trichlorfon solution against ivermectin resistant nematode strains in cattle

Infected calves from two different rural estates in Brazil were studied to assess the anthelmintic efficacy of oral trichlorfon against naturally occurring ivermectin resistant parasitic nematode strains. In experiment 1, infected animals were from a region where ivermectin resistant populations of Haemoncus placei, Cooperia punctata,Cooperia spatulata and Trichuris discolor have recently been identified. Six calves with natural gastrointestinal nematode infections were treated with 48.5 mg/kg aqueous trichlorfon administered orally and six calves acted as a non-treated control group. In experiment 11 24 naturally infected calves were selected to enter one of four treatment groups, six animals each received: 48.5 mg/kg oral trichlorfon; 200 mu g/kg subcutaneous 1% ivermectin; 630 mu g/kg subcutaneous 3.15% ivermectin; or no treatment (control group). Gastrointestinal helminths were counted and identified post-mortem at 7 days (trichlorfon and 1% ivermectin treated and untreated animals) or 14 days (3.15% ivermectin treated and untreated animals) after administration of the test agents. Experiment I identified a high level efficacy for oral trichlorfon against four helminth species that have previously been shown to be ivermectin resistant in this geographical region: percentage efficacy was 99.82% against adult H. placei, 99.18% against C. punctata, 99.33% against C. spatulata, 81.06% against T. axei, 98.46% against Oesophagostomum radiatum and 100% against T. discolor. Trichlorfon also showed activity against the ivermectin (1% and 3.15%) resistant helminth species identified in experiment 11, attaining efficacy levels of 99.17% against H. placei, 98.46% against C punctata and 100.00% against T. discolor. These findings indicate that oral trichlorfon is an effective treatment option in the management of cattle infected with ivermectin resistant helminths. (C) 2009 Elsevier B.V. All rights reserved.

Estudo morfométrico comparativo de 58 populações brasileiras de Rotylenchulus reniformis (Nemata: Rotylenchulinae)

Foram recuperadas 58 populações de Rotylenchulus reniformis de amostras de solo e raízes de diferentes culturas e inoculadas em plantas de algodoeiro (Gossypium hirsutum) cv. COODETEC 402 e de mamona (Ricinus communis), mantidas em vasos de argila em casa de vegetação do Departamento de Fitossanidade da UNESP/FCAV, Campus de Jaboticabal - São Paulo. Foi realizado estudo morfométrico comparativo das populações ao microscópio óptico composto, seguido da ordenação das populações segundo análises multivariadas de agrupamento e de componentes principais. Foram avaliadas 11 variáveis morfométricas em dez fêmeas jovens de cada população e sete variáveis derivadas. A amplitude de variação de caracteres morfométricos em populações brasileiras desse nematóide tais como, comprimento do estilete, V e forma da cauda, é maior que em populações da mesma espécie de outras regiões do mundo. Os dados obtidos confirmam que o comprimento do estilete, presença de machos e V são suficientes para identificação de R. reniformis e que esta é a espécie do grupo predominante nos agroecossistemas brasileiros.

Histopathological and histomicrobiological study of root canal therapy medication, comparison of calcium hydroxide versus gutta-percha with zinc oxide/eugenol in the teeth of dogs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Steinernema brazilense n. sp (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil

A new entomopathogenic nematode, Steinernema brazilense n. sp., was isolated from a single soil sample collected from a natural forest in Mato Grosso do Sul state, Brazil. S. brazilense n. sp. is characterized morphologically by features of infective juveniles (IJ), males and females. For the IJ, body length averaging 1157 (1023-1284) mu m, distance from anterior end to excretory pore 95 (87-102) mu m, from anterior end to end of esophagus 148 (139-153) mu m, tail length 85 (80-104) mu m, D% and E% values 63 (58-70) and 106 (95-118.0), respectively. Lateral field pattern variable; the formula for the arrangement of ridges from head to tail is: 2, 4, 6, 8, 6, 2. For the male, the diagnostic characters include spicule averaging 83 (75-89) mu m; D% about 65; the ratio SW% about 192. The length of spicule head is greater than width. Lateral field with one narrow ridge. First generation females are characterized by the presence of a ventral postanal swelling. S. brazilense n. sp. is morphologically close to Steinernema diaprepesi. It can be differentiated from S. diaprepesi by its longer IJ body length (1157 vs 1002 mu m), longer distance from anterior end to excretory pore (1110 vs 75 mu m), a longer tail length (103 vs 83 mu m); males of the new species with longer spicule (83 vs 79 mu m). The new species can be distinguished further from other members of Steinernema glaseri group by characteristics of rDNA of ITS and D2D3 regions. Published by Elsevier B.V.

Estudo morfométrico comparativo de populações de Rotylenchulus reniformis (Nemata: Rotylenchulinae) do Brasil

Quarenta e oito populações de Rotylenchulus reniformis foram recuperadas de amostras de solo e raízes de diferentes culturas e inoculadas em diferentes plantas hospedeiras, mantidas em microparcelas no Departamento de Fitossanidade da UNESP/FCAV, Campus de Jaboticabal, São Paulo. Os sintomas da doença causada pelo nematóide em algodoeiro (Gossypium hirsutum), no campo, foram documentados, bem como o hábito de parasitismo do nematóide em raízes de algodão e de mamoeiro (Carica papaya), utilizando-se de coloração in situ do nematóide com fuccina ácida. Efetuou-se a comparação morfológica de todas as populações, ao microscópio óptico composto, em montagens temporárias e, de algumas, ao microscópio eletrônico de varredura. Para as observações ao microscópio eletrônico de varredura, fêmeas adultas presas às raízes foram fixadas em glutaraldeído e pós-fixadas em tetróxido de ósmio, desidratadas em álcool etílico, secas em secador de ponto crítico, montadas, recobertas com 35 nm de ouro, observadas e elétromicrografadas em 15 kV. Os dados obtidos confirmam que R. reniformis é a única espécie do gênero distribuída nos agroecossistemas brasileiros e que a amplitude de variação de caracteres morfométricos em populações brasileiras desse nematóide, tais como comprimento do estilete, V % e forma da cauda, é maior que em populações da mesma espécie de outras regiões do mundo. Foram ilustradas fêmeas jovens de R. reniformis com a cauda bifurcada, e esse detalhe da morfologia do nematóide ainda não havia sido relatado.

Pterygodermatites (Multipectines) pluripectinata n. sp. (Spirurida: Rictulariidae), a nematode parasite of the crab-eating fox Cerdocyon thous (Linnaeus, 1766) from Caatinga shrubland, Brazil

In a parasitological survey of free-ranging Cerdocyon thous (Carnivora: Canidae) from Brazilian Caatinga shrubland, a new species of Pterygodermatites (Multipectines) was recovered from the small intestine of this host. Morphological analysis showed that P. (Multipectines) pluripectinata n. sp. is distinguished from all other congeneric species mainly by the numerous plate-like projections and male caudal morphology and spicular length. There are few records on the occurrence of this genus in Neotropical regions.

Root system of Panicum maximum Jacq. cv. IPR-86 Mil(e)over-capnio fertilized with nitrogen and submitted to rotational stocking

The effect of nitrogen on the root system of the species Panicum maximum Jacq. cv. IPR-86 Mil (e) over cap nio, under grazing, was evaluated. The N rates were 0; 150; 300 and 450 kg/ha. year. The root density was evaluated during pregrazing at five years of successive N application, in three depths (0-10; 10-20 and 20-40 cm) and the root growth at 7, 14, 21, and 35 days after grazing. The grazing method adopted was rotational stocking. Root length and root mass densities in pre-and post-grazing presented maximum values at rates 204, 206, 192, and 197 kg/ha of N, respectively. The root growth (in root length density) increased, on average, until 29 days after grazing at rates 0, 150, and 300 kg/ha; at 450 kg/ha N rate, the increase was linear. Independently of N rates, around 60 and 25% of IPR-86 Mil (e) over cap nio cultivar root system was concentrated in 0-10 and 10-20 cm depth, respectively.