Gestational and lactational exposition to Di-N-butyl-phthalate (DBP) increases inflammation and preneoplastic lesions in prostate of wistar rats after carcinogenic N-methyl-N-nitrosourea (MNU) plus testosterone protocol

In the present study, it was evaluated the susceptibility of prostatic lesions in male adult rats exposed to Di-N-butyl-phthalate during fetal and lactational periods and submitted to MNU plus testosterone carcinogenesis protocol. Pregnant females were distributed into four experimental groups: CN (negative control); CMNU (MNU control); TDBP100 (100 mg/kg of DBP); TDBP500 (500 mg/kg of DBP). Females from the TDBP groups received DBP, by gavage, from gestation day 15 (GD15) to postnatal day 21 (DPN21), while C animals received the vehicle (corn oil). CMNU, TDBP100, and TDBP500 groups received a single intraperitoneal injection of MNU (50 mg/kg) on the sixth postnatal week. After that, testosterone cypionate was administered subcutaneously two times a week (2 mg/kg) for 24 weeks. The animals were euthanized on PND220. Distal segment fragments of the ventral (VP) and dorsolateral prostate (DLP) were fixed and processed for histopathological analysis. Protein extracts from ventral prostate were obtained, and western blotting was performed to AR, ERα, MAPK (ERK1/2), and pan-AKT. Stereological analysis showed an increase in the epithelial compartment in TDBP100 and TDBP500 compared to CN. In general, there was increase in the incidence of inflammation and metaplasia/dysplasia in the DBP-treated groups, mainly in DLP, compared to CN and CMNU. Proliferation index was significant higher in TDBP500 and PIN (prostatic intraepithelial neoplasia) was more frequent in this group compared to CMNU. Western blot assays showed an increase in the expressions of AR and MAPK (ERK1/2) in the TDBP100 compared to CN, and ERα and AKT expressions were higher in the TDBP500 group compared do CN. These results showed that different doses of DBP during prostate organogenesis in Wistar rats could increase the incidence of premalignant lesions in initiated rats inducing distinct biological responses in the adulthood. © 2015 Wiley Periodicals, Inc. Environ Toxicol, 2015.

Criptorquidia e exposição in utero ao di(n-butil)-ftalato e à acrilamida: avaliação morfológica do dano testicular

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise das capas de livros de Vicente Di Grado: década de 1960

This article intends to contribute to the Brazilian design history through a study of a selection of book covers produced in 1960 decade by Vicente Di Grado (1922 - 2004), who was the main cover artist and illustrator of a publishing house named Clube do Livro. The covers were analysed considering their visual and contextual syntax and in their relations with information design.

Capas de Vicente Di Grado na década de 1960

O resgate da história e da memória do design gráfico brasileiro ainda possui algumas lacunas, pois muitos profissionais, seus projetos e obras não são lembrados ou ainda não foram estudados e registrados, deixando uma lacuna na história gráfico-visual de nosso país. Este artigo pretende contribuir nesse sentido, resgatando as capas de livros projetadas por Vicente Di Grado. Designer gráfico, artista plástico e docente, Di Grado (1922-2005) atuou como principal capista para a Editora Clube do Livro entre as décadas de 1950 e 1970, sendo seu maior período de produção a década de 1960, justamente o objeto deste estudo. Pelo conjunto de seu trabalho, recebeu o Prêmio Jabuti em 1963. Sua obra representa uma grande fonte de elementos e referências gráficas, além de ter marcado a linguagem editorial da Editora Clube do Livro.