Preference and infestation level of Anastrepha fraterculus (Wiedemann, 1830) on fruits of some Psidium guajava L. cultivars and relation to their physicochemical parameters

This study aimed to verify the preference and infestation level of Anastrepha fraterculus (Diptera: Tephritidae) [South American fruit fly] in fruits of guava cultivars and to correlate them to variables such as peel coloration, soluble solids and pH of fruit cultivars. The following cultivars were used: Pedro Sato, Paluma, Casco and S,culo XXI. The infestation was evaluated in cages, considering two scenarios: no-choice and multiple choice. In both tests, evaluations of the fruit attraction to insects were conducted for a period of 1', 3', 5', 10', 20', 30', 1 h, 2 h, 6 h, 12 h and 24 h. The visit of A. fraterculus on the assayed cultivars in relation to the time was studied by logistic regression. After 10 days, the number of larvae in each fruit was recorded. In the multiple choice test, the visit proportions were significantly higher in the fruits of cvs. S,culo XXI and Pedro Sato than in those of cvs. Paluma and Casco. In the no-choice test, the visit proportions were significantly lower in the Paluma fruits. In both tests, the rate of fruit infestation by A. fraterculus did not differ among cvs. Pedro Sato, Paluma and Casco, whereas the fruits of cv. S,culo XXI were more infested. The indexes of pH did not interfere with the infestation of A. fraterculus, whereas a high rate of soluble solids and low color angle appear to be crucial for discriminating the fruits of the most susceptible cultivars. Infestation rate of S,culo XXI fruits displayed significant correlations with: A degrees Brix (r= 0.7078) and color angle (h) (r= -0.9499) of guava fruits under the multiple choice conditions.

Investigation on the s of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa

Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa (purple) - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than that of the infusions of the varieties Paluma and Pedro Sato. It was observed that seed germination and root growth in L. sativa exposed to infusions decreased with increasing infusion concentration, regardless of the tested cultivar. For the mitotic index, no statistical differences were observed. On the other hand, a significant increase in the frequency of cell cycle alterations was verified, especially for the highest concentrations tested. The cytogenotoxic was significant. Therefore, guava should not be used indiscriminately in popular medicine.

Adubação nitrogenada e potássica em goiabeiras ‘paluma’: II. efeito no estado nutricional das plantas

The proper mineral fertilization of guava orchards under intensive management, is a major factor in productivity and monitoring the nutritional status of the plants contributes to the efficiency of this agronomic practice. The aim of this study was to investigate different doses of nitrogen and potassium in the nutritional status of guava 'Paluma'. The experiment was conducted at Vista Alegre do Alto, Sao Paulo, in a 7 year old irrigated orchard, managed with fruiting pruning during four consecutive growing seasons. The soil is a dystrophic Ultisol. The experimental design was randomized blocks, in factorial, with four nitrogen doses (0, 0.5, 1 and 2 kg of N plant(-1)) and four of potassium (0, 0.55, 1.1 and 2.2 kg of K2O plant(-1)), with three replications. Nitrogen fertilization promoted increased levels of leaf N and Mn and the decrease in the levels of P and B; effects observed from the second to the fourth production cycle. Nitrogen fertilization increased the Ca and Mg levels, respectively, in the second and third cycle. Except for the first production cycle, K and Mn foliar concentrations increased with increasing potassium fertilization, whereas the levels of Mg, in the second and the fourth cycle, decreased as a function of fertilization.

Adubação nitrogenada e potássica em goiabeiras ‘paluma’: I. efeito na produtividade e na qualidade dos frutos para industrialização

The nutrition of the orchards is the major factor of productivity, being necessary to know the proper doses of fertilizers and their influence on fruit quality attributes for industrialization. This study evaluated the effects of different doses of nitrogen and potassium on the productivity of guava trees and also on the values of pH, soluble solids (SS), titratable acidity (TA) and pulp/kernel ratio of guavas. The experiment was conducted at Vista Alegre do Alto, SP in an irrigated 'Paluma'guava orchard, 7 years old, managed with pruning during three consecutive cycles of production. The soil of the area was dystrophic Ultisol. The experimental design was the randomized blocks, in factorial, with four nitrogen doses (0, 0.5, 1 and 2 kg of N plant(-1)) and four of potassium (0, 0.55, 1.1 and 2.2 kg of K2O plant(-1)), with three replications. Nitrogen fertilization increased productivity and the pH of the fruit, being explained by the quadratic polynomial regression models; reduced linearly the pulp/kernel ratio and do not influenced the SS and TA values. On the other hand, potassium fertilization and N x K interaction had no significant effects on productivity and the other characteristics evaluated.

Infestation of guava by Anastrepha fraterculus (Diptera: Tephritidae): preferred ripening stages and influence of fruit peel coloration

The current study aimed to verify the preferred guava fruit ripening stage for egg laying by Anastrepha fraterculus (Diptera: Tephritidae) and to determine the influence of peel coloration of the fruits of 2 cultivars on infestation. The cultivars used were Paluma and Seculo XXI. The infestation level was evaluated in cages, considering 2 conditions: multiple-choice and nonchoice tests. The infestation levels of A. fraterculus differed between the green and the green-ripe stages in the multiple-choice test. In Paluma fruits with the nonchoice test, the infestation was highest during the ripe stage. There were no differences in infestation levels of A. fraterculus between the green and the green-ripe stages in Seculo XXI. In general, the green-ripe and the ripe stages were preferred for egg laying by A. fraterculus. In Paluma fruits there were significant correlations of infestation levels of A. fraterculus with luminosity (L) and chromaticity (C) in the nonchoice assay. In Seculo XXI fruits there was a significant correlation of infestation levels of A. fraterculus with color angle (h) in the nonchoice assay. No correlation was found between theses parameters in the multiple-choice test.

Produtividade de alface cv Lucy Brown influenciada por períodos de convivência com plantas infestantes e potencial alelopático da tiririca

This work was divided in two experiments, the first one to evaluate the lettuce productivity (cv Lucy Brown - American group) influenced by increasing and decreasing periods of coexisting with harmful plants, and a second moment, aiming at to evaluate the allelopathic potential of nutsedge (Cyperus rotundus L.) on the initial lettuce development.The experimental treatments constituted of six increasing and decreasing periods of coexisting with harmful plants, or control of it in the culture, considered from the plantation of the lettuce; being separate in two groups: Weeds and Clean weeds.In the second moment of the experiment, purple nutsedge aerial parts had been collected in the area of experiment 1, that drying had been after triturated and immersed in methylic alcohol P. A in ratios 10, 5,0 and 2.5% (w/ v) and later impregnated in germination paper, where had been placed lettuce seeds.The analyses of results, one concludes that the presence of C. rotundus plants can intervene with the germination, growth and development of the plants of lettuce cv Lucy Brown, and that initial periods of coexisting between the culture and the harmful are associate to an induction of the foliar area development, and can not express the reduction of productivity in the end of the cycle. Drawn out periods of competition induce the reduction of the lettuce foliar area, and then they will intervene with the yield productivity.

Alterações anatômicas induzidas pelo cálcio na parede celular de frutos de goiabeira

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

MICROPROPAGAÇÃO do KIWI CV. HAYWARD

O presente trabalho teve como objetivo desenvolver um protocolo para a obtenção de mudas de kiwi (Actinidia chinensis Planch), cv. Hayward, por meio do cultivo in vitro de cotilédones. Utilizou-se o meio de MURASHIGE & SKOOG (1962) -- MS, suplementado com dois tipos de auxina (AIA e AIB) e uma citocinina (BAP). Foram verificados os efeitos de três doses de auxinas (0,125; 0,250 e 0,375 mg.L-1), combinadas com três doses de citocinina (0,5; 1,0 e 1,5 mg.L-1) na capacidade morfogênica dos explantes. Procedeu-se o estudo histológico dos órgãos das plântulas obtidas in vitro, e verificou-se, também, a capacidade de aclimatação das mudas ex vitro. A menor dose de AIB (0,125 mg.L-1), independentemente das doses de BAP, foi a mais eficaz na morfogênese dos explantes. Não foram verificadas alterações histológicas e anatômicas das plântulas obtidas in vitro. Aos três meses após o cultivo ex vitro dos explantes, verificou-se a sobrevivência de 88% das plantas transplantadas em condições de campo.

Qualidade física e fisiológica de sementes da leguminosa forrageira Macrotyloma axillare cv. Java

É de interesse prático, quando se dispõe de diferentes lotes de sementes, conhecer a qualidade fisiológica intrínseca a cada um. Objetivou-se determinar a qualidade fisiológica de lotes de sementes da leguminosa forrageira tropical, Macrotyloma axillare cv. Java, com utilização de diferentes metodologias para realização dos testes germinação e vigor. Determinou-se a pureza física dos lotes, o peso de mil sementes, a germinação com e sem escarificação (TG) e o vigor (índice de velocidade de germinação (IVG), primeira contagem, envelhecimento acelerado e condutividade elétrica) de três lotes de sementes. Diferenças na qualidade fisiológica inicial de sementes escarificadas foram observadas pelo teste de germinação. Pelos resultados dos testes de primeira contagem e IVG não foi possível detectar diferenças na qualidade fisiológica das sementes; o envelhecimento acelerado das sementes escarificadas pode ser realizado a 41º C por 72 horas ou a 45º C por 48 horas; o teste de condutividade elétrica mostrou-se adequado para diferenciar os lotes, a partir de 48 horas de embebição.

Influência do cultivar, do tipo de folha e do tempo de cultivo na medida indireta da clorofila (spad) em mudas de goiabeira

A análise foliar de nitrogênio, comumente empregada em laboratórios de rotina, é destrutiva e nem sempre rápida, obstante para a tomada de decisão. Objetivou-se com a pesquisa estudar as medidas SPAD, em mudas de goiabeira (Psidium guajava L.) cultivada em solução nutritiva completa, observando-se as diferenças entre cultivares, épocas de amostragem e tipos de folhas avaliadas. O delineamento foi inteiramente casualizado, com quatro repetições, em parcelas sub-subdivididas. Assim, foi utilizada como parcela as duas cultivares de goiabeira (Paluma e Século XXI), como subparcelas os tipos de folhas (+1, +2 e +3) e, cinco épocas de amostragem ao longo do período experimental (60, 75, 90, 105 e 120 dias, após o transplantio de mudas) Concluiu-se que a leitura SPAD em mudas de goiabeira é influenciada pela cultivar, tipo de folha e época de amostragem. O terceiro par de folhas mostrou-se mais adequado para avaliar o estado nutricional do nitrogênio nas mudas de goiabeira a partir da leitura SPAD. As cultivares de goiabeira apresentaram diferença na leitura SPAD apenas aos 60 e 90 dias após o transplantio, independentemente do tipo de folha.

Técnicas de análise multivariada na avaliação de injúrias pelo frio em mangas cv. Tommy Atkins

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Curvas de crescimento do fruto de cafeeiro cv. Obatã IAC 1669-20 em diferentes alinhamentos de plantio

The growth of the coffee fruit is highly dependent on physiological and environmental factors. Environmental factors that most influence the yield of coffee during the critical growth stages are the minimum and maximum temperatures of air, which are affected by solar radiation and depend on the apparent path of the sun. This research work correlated dry mass versus fresh mass, and, fresh and dry mass as function of days after flowering of fruit coffee cv. Obata IAC 1669-20 planted in three different alignments based on the apparent trajectory of the sun, in Jaboticabal, SP. The planting was aligned according to the apparent path of the sun. There were three treatments with four replications, a total of twelve plots, each plot with one hundred plants of coffee, each treatment corresponded to an alignment of planting. The three alignments used showed no differences with respect the average fresh and dry mass. The growth rate of fruit, in fresh weight, was higher in the exponential phase, and in dry mass was higher in the linear stage. The dry weight (DW) can be estimated from the fresh weight (FW) using the model: DW = A1*exp(FW/t1). The sigmoidal model that represents the fruit growth in of coffee plants in fresh and/or dry mass in terms of days after flowering (DAF) was: FW(DW) = A2+[(A1-A2)/(1+exp(DAF-x0)/dx)].

Redução de aplicações de inseticidas através da adoção de táticas de manejo integrado do Triozoida limbata (Enderlein, 1918) (Hemiptera: triozidae) em goiabeira

The guava tree pest that has been requesting larger control frequency, in the area of Jaboticabal - São Paulo in the last years, is the psilideo Triozoida limbata. Thus, the objectives of this research were: a) Compare control tactics T. limbata based on monitoring and selectivity of insecticide to decrease the applications; b) To register and to correlate the natural enemies in habitants in guava tree ecosystem with the key pest and with meteorological factors; c) See if the control of T. limbata affects the population of fruit flies and d) To verify the efficiency of appropriate insecticides to IMP, in control of T. limbata. The experiment was conducted in 2004, in an orchard of Paluma cultivar in Vista Alegre do Alto, SP. The used strategies were (doses in g.a.i./100L water): control; imidacloprid + beta-cyfluthrin (2.5 + 0.3); acetamiprid (4.0); fenpropathrin (15.0) and regional conventional treatment. It was concluding that is possible to reduce the number of applications of less aggressive insecticides to the environment and the man, with the adoption of monitoring of T. limbata and implementing the action level. The natural enemies' populations densities (Scymnus spp., Cycloneda sanguinea, Azia luteipes, Crysoperla spp., Polybia spp., Brachygastra ssp.) present positive correlations with the populations of T. limbata. Population fluctuations of T. limbata and natural enemies are not altered by meteorological factors (precipitation and temperature) in irrigated orchard. The population of Anastrepha spp. is minimized when of the applications is for control T. limbata. The insecticides imidacloprid, imidacloprid + beta-cyfluthrin, acetamiprid and fenpropathrin are efficient in the control of T. limbata.

Amostragem, diversidade e sazonalidade de Hemerobiidae (Neuroptera) em Coffea arabica L. cv. Obatã (Rubiaceae)

Este estudo teve por objetivo avaliar métodos de amostragem, abundância sazonal e diversidade da população de Hemerobiidae associada a cultivo de café Coffea arabica L. cv. Obatã em Cravinhos, São Paulo, Brasil. Para tanto foram realizadas amostragens semanais no período de maio de 2005 a abril de 2006. Os métodos de amostragem utilizados foram: rede de varredura e armadilhas de Möericke e luminosa. Foram coletados 491 exemplares de Hemerobiidae pertencentes a quatro gêneros: Nusalala (231 espécimes / 47,2% do total de hemerobiídeos coletados), Megalomus (110 / 22,5%), Hemerobius (104 / 21,3%) e Sympherobius (44 / 9%). A rede de varredura foi a mais eficiente para a captura de Hemerobiidae e a armadilha de Möericke foi o método de amostragem que apresentou os maiores valores de diversidade (H'= 0,56) e de equitabilidade (J= 0,93). Os hemerobiídeos estiveram presentes na área estudada durante o ano todo; as maiores freqüências foram registradas entre agosto e março (final do inverno, primavera e verão) e o maior pico populacional ocorreu em janeiro (na metade do verão). Megalomus apresentou correlação positiva e significativa (p< 0,05) com a precipitação pluviométrica e as temperaturas máxima e mínima; Nusalala com as temperaturas máxima e mínima e, Sympherobius apenas com a temperatura máxima.

Influência do envelhecimento acelerado na anatomia da testa de sementes de soja, cv. Monsoy 8400

Durante o processo de envelhecimento, as sementes sofrem alterações degenerativas que influenciam o seu potencial fisiológico. Entre estas alterações podem estar as modificações anatômicas nas camadas celulares da testa da semente. Objetivou-se, com este trabalho, verificar a relação entre a anatomia da testa de sementes de soja, cultivar Monsoy 8400 e a germinação, após o envelhecimento acelerado, pelo fato da testa apresentar um papel fundamental na conservação do potencial fisiológico das sementes. As sementes foram envelhecidas a 42ºC e 100% UR do ar por períodos de zero, 24, 48, 72 e 96 horas. Avaliaram-se os teores de água e a germinação, posteriormente à secagem, seções transversais da testa de 10 sementes de cada tratamento foram eletromicrografadas, com microscópio eletrônico de varredura,,observando-se as camadas das células paliçádicas, ampulhetas e parenquimáticas A germinação declinou com o aumento dos períodos de envelhecimento. Houve redução da espessura das camadas da testa nos períodos de 48, 72 e 96 horas deste processo. Observou-se que, principalmente, para os períodos de envelhecimento de 72 e 96 horas, que as células da camada hipodérmica apresentaram um aspecto de colapso, sendo este acentuado para o período de 96 horas. A redução da espessura das camadas sugere a ocorrência de um colapso das células que compõem tais camadas, sendo um fator causador da redução do potencial germinativo.