Avaliação das citocinas (ELISA e RT-PCR) e da fibrose hepática na coinfecção pelo HIV e vírus da hepatite C

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo do limiar de positividade do método imunoenzimático (ELISA) para pesquisa de coproantígenos de Giardia lamblia Stiles, 1915. Sua utilização como exame de controle de cura após terapêutica

Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR

Ensaios imunoenzimáticos (ELISA) para detecção da resposta sorológica contra Salmonella Gallinarum, Salmonella Pullorum, Salmonella Enteritidis e Salmonella Typhimurium em aves

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência das substâncias húmicas aquáticas na determinação de atrazina por imunoensaio (Elisa)

Pós-graduação em Química - IQ

Padronização e aplicação da técnica de ELISA (Enzyme-linked immunosorbent assay) indireto com anticorpo de captura para a detecção de anticoepos contra o cicovírus suíno tipo 2

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Desenvolvimento e aplicação do ELISA indireto com proteína recombinante de nucleocapsídeo de coronavírus felino (FCoV) para quantificação de anticorpos em soros de gatos naturalmente infectados

Pós-graduação em Medicina Veterinária - FMVZ

Padronização do Elisa indireto e Western Blot para diagnóstico da artrite-encefalite caprina

Caprine arthritis-encephalitis (CAE) is routinely diagnosed with the Agarose Gel Immunodiffusion (AGID) technique, which is considered to have low sensitivity. The objective of this study was to standardize testing i-Elisa and Western Blot for early detection of antibodies against CAEV in goats and compare the results obtained in these tests with proof of AGID. For standardization of i-Elisa and WB, different concentrations and dilutions of antigen, sera and conjugate were used. In the i-Elisa, rigid microplate with 96 wells was adopted, and the combination that showed the best result was a concentration of 0.5µg/ well of antigen and dilutions of the serum of 1:100 and conjugate of 1:1500. In the WB nitrocellulose membranes were used, and the dilutions of the serum were defined at 1:50 and conjugate at 1:15000. To evaluate the performance of the techniques, 222 goat serum samples were tested and the data were compared with the AGID. The sensitivity and specificity of Elisa-i/IDGA, WB/AGID and WB/Elisa-i were 70% and 91%, 100% and 72.6%, 84.6% and 76.5%, concomitantly. The Kappa index of these tests was 0.35, 0.2 and 0.36, respectively. The i-Elisa and WB techniques were more sensitive than the AGID and can be used as tools for early diagnosis of CAE.

Dengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Development of a peptide ELISA for the diagnosis of Equine arteritis virus

A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations. (C) 2014 Elsevier B.V. All rights reserved.

Subsídios para o plano de manejo da futura Estação Ecológica Santa Elisa

O presente estudo tem como objetivo reunir os dados existentes sobre a área da futura Estação Ecológica Santa Elisa, localizada no Centro Experimental Central do Instituto Agronômico (IAC), em Campinas – SP. Assim, foram reunidos dados sobre os aspectos bióticos (fauna e flora) e abióticos (clima, geologia e geomorfologia, hidrografia e solos), resultando em uma caracterização da área como forma de subsídio para o plano de manejo. Através de saídas a campo foram levantados problemas que prejudicam a caracterização de uma estação ecológica, surgindo assim um segundo objetivo, adequar a área da futura unidade para o melhor enquadramento em tal categoria. Foram então elaborados projetos para melhorar o estado de conservação da futura unidade, sempre levando em consideração a falta de recursos. Sendo assim recomenda-se parcerias com universidades para que dessa forma se consiga implantar os projetos e realizar pesquisas de forma mais econômica. Embora o IAC tenha um histórico de pesquisas voltado apenas para a agricultura, a preocupação ambiental vem aumentando e a criação dessa unidade de conservação garante que se continue preservando os remanescentes presentes no IAC mesmo após a mudança de sua gestão.

Desenvolvimento e aplicação do Elisa indireto com a nucleoproteína recombinante (NPR) do vírus da doença de Newcastle

Pós-graduação em Medicina Veterinária - FCAV

Subclinical diagnosis of Caseous lymphadenitis based on Elisa in Sheep from Brazil

Caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis, is a chronic contagious disease that affects small ruminants and still remains an important problem for many lamb-producing countries. Animals are considered clinically infected when occurs abscesses in superficial lymph nodes. Visceral or internal form can coexist which no apparent clinical signs of infection are seen. The best procedure to avoid spread of the disease is elimination of infected animals. However, as the chronic and subclinical nature of the infection of CLA alternative methods are required for detection and screening. In this study, we described the performance of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for diagnosis of CLA in asymptomatics sheep. Also, test culture and biochemical identification were achieved to confirm CLA infection. The serological diagnostic was performed in sheep symptomatics (n=50) and asymptomatics (n=374) from nine flocks. Analysis reported high positivity of 71% for ELISA in 85% of asymptomatic animal for CLA with a sensitivity of 88% and specificity of 31%. Results from ELISA test in asymptomatic animals against culture for caseous lymphadenitis were more specific (97%) and permitted to exclude healthy animals without symptoms. This study concluded that C. pseudotuberculosis infection could be widely disseminated in sheep flocks in Northwestern region of the state of São Paulo, Brazil and only one screening test is not enough. The association with indirect ELISA test and culture could better indicate the real problem of CLA in sheep flocks.

Importância da avaliação hematológica e sorológica (dot-blot ELISA) no diagnóstico de erliquiose em cães

This study compared the hematological and serological analysis of diagnosis of canine ehrlichiosis. The survey of Ehrlichia canis was performed through the evaluation of blood smears from 150 dogs. The serological test was performed on 12 samples selected by the platelet count (less than 170,000 platelets / uL). Serologic testing was performed with the Imunocomb kit - Dot-blot-ELISA. No cytoplasmatic inclusion characteristic of morula of E. canis was found in blood smears. In serologic testing, eight samples were positive for Ehrlichia canis, concluding that thrombocytopenia is an important hematological finding of ehrlichiosis diagnosis and the detection of Ehrlichia canis morulae is uncommon. The serological evaluation Dot-blot ELISA is an accurate and brief diagnosis method of canine ehrlichiosis, been the most appropriate to be used in veterinary practice routine.

Quantificação do fator de crescimento semelhante a insulina I (IGF-I) em plasma bovino por ELISA

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do teste de imunodifusão dupla em gel de agar e do ensaio imunoenzimático - ELISA no diagnóstico e seguimento de pacientes com bola fúngica aspergilar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)