Effects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle

FSH induces expansion of bovine cumulus-oocyte complexes (COCs) in cattle, which can be enhanced by oocyte-secreted factors (OSFs). In this study it was hypothesised that FSH stimulates COC expansion in part from direct stimulation of the epidermal growth factor (EGF)-like ligands amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC), but also in part through regulation of OSFs or their receptors in cumulus cells. Bovine COCs were cultured in defined medium with graded doses of FSH. In the absence of FSH, COCs did not expand. FSH caused cumulus expansion, and increased the abundance of AREG and EREG mRNA in a time- and dose-dependent manner, but decreased BTC mRNA levels. FSH had modest stimulatory effects on the levels of mRNA encoding the bone morphogenetic protein 15 (BMP15) receptor, BMPR1B, in cumulus cells, but did not alter mRNA expression of the growth and differentiation factor 9 (GDF9) receptor, TGFBR1. More interestingly, FSH dramatically stimulated levels of mRNA encoding two receptors for fibroblast growth factors (FGF), FGFR2C and FGFR3C, in cumulus cells. FSH also stimulated mRNA expression of FGFR1B, but not of FGFR2B in cumulus cells. Based on dose-response studies, FGFR3C was the receptor most sensitive to the influence of FSH. This study demonstrates that FSH stimulates the expression of EGF-like factors in bovine cumulus cells, and provides evidence that FSH differently regulates the expression of distinct receptors for OSFs in cumulus cells. © CSIRO 2013.

Análise de multirresolução baseada em polinômio potência de Sigmóide - Wavelet

Pós-graduação em Ciência da Computação - IBILCE

Composição de dicionários visuais utilizando agrupamento de dados por Florestas de Caminhos Ótimos

Pós-graduação em Ciência da Computação - IBILCE

Sistema de monitoração de bloqueios tipo porta de vidro

Automatic video surveillance system has been a frequent topic of research due to the large number of promising applications. In this research, we developed a tracking and counting people system, as well as suspicious activities detector. The model tracks individual objects as they pass through the field of vision of the camera using vision algorithms to classify the activities of each person, and according to this features, detect dangerous situations. This dissertation includes a review of several techniques trying to develop a robust and low computacional costs system to be used in glass door barrier turnstiles avoiding fraud

A dimensão afetiva nas práticas pedagógicas de professoras alfabetizadoras dos anos iniciais do ensino fundamental

Pós-graduação em Educação Escolar - FCLAR

Tecnologias virtuais e análise videográfica: o YouTube® como recurso de pesquisa para compreensão sobre a imagem do idoso brasileiro = Virtual technologies and videographic analysis : YouTube® as research resource for understanding about brazilian elderly imagem

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Visão estéreo para auxílio à navegação autônoma em ambientes agrícolas

Pós-graduação em Ciência da Computação - IBILCE

Uma metodologia para a geração de conteúdos digitais baseado na utilização de estudios virtuais com realidade aumentada

Pós-graduação em Televisão Digital: Informação e Conhecimento - FAAC

Swarm-based descriptor combination and its application for image classification

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estimativa do número de frutos verdes em laranjeiras com o uso de imagens digitais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Segmentação interativa de imagens utilizando competição e cooperação entre partículas

Pós-graduação em Ciência da Computação - IBILCE