Exercise at anaerobic threshold intensity and insulin secretion by isolated pancreatic islets of rats

To evaluate the effect of acute exercise and exercise training at the anaerobic threshold (AT) intensity on aerobic conditioning and insulin secretion by pancreatic islets, adult male Wistar rats were submitted to the lactate minimum test (LMT) for AT determination. Half of the animals were submitted to swimming exercise training (trained), 1 h/day, 5 days/week during 8 weeks, with an overload equivalent to the AT. The other half was kept sedentary. At the end of the experimental period, the rats were submitted to an oral glucose tolerance test and to another LMT. Then, the animals were sacrificed at rest or immediately after 20 minutes of swimming exercise at the AT intensity for pancreatic islets isolation. At the end of the experiment mean workload (% bw) at AT was higher and blood lactate concentration (mmol/L) was lower in the trained than in the control group. Rats trained at the AT intensity showed no alteration in the areas under blood glucose and insulin during OGTT test. Islet insulin content of trained rats was higher than in the sedentary rats while islet glucose uptake did not differ among the groups. The static insulin secretion in response to the high glucose concentration (16.7 mM) of the sedentary group at rest was lower than the sedentary group submitted to the acute exercise and the inverse was observed in relation to the trained groups. physical training at the AT intensity improved the aerobic condition and altered insulin secretory pattern by pancreatic islets. © 2010 Landes Bioscience.

Endurance swimming periodized training in rats

The aim of this study was to develop an experimental protocol for endurance swimming periodization training in rats similar to high performance training in humans, and compare it to continuous training. Three groups of male Wistar rats (90 days old) were allocated to Sedentary Control (SC); Continuous Training (CT); and Periodized Experimental Training (PET) groups. PET and CT trained 5 days/week, over five weeks, CT: continuous training supporting a 5% body mass (bm) load for 40 min/day; PET: training subdivided into basic, specific, and taper periods, with overload changed daily (volume-intensity, continuous, and interval training). Total training overload was quantified (% bm X exercise time in training session) and equalized for the two trained groups. Glucose ([ 3H]2-deoxyglucose) uptake, incorporation to glycogen (synthesis), glucose oxidation (CO 2 production), and lactate production from [U- 14C]glucose by soleus muscle strips incubated in presence of insulin (100μU/mL) were evaluated 48h after the last training session. The load equivalent at 5.5mM blood lactate concentration ([La-5.5]) was determined in the incremental test. Lactate production was similar in all groups. PET presented higher glucose uptake (59%) than SC, and higher glycogen synthesis (51 and 22%) and glucose oxidation (147 and 178%) than SC and CT, respectively. CT presented higher glycogen synthesis rates (23%) than SC. Load [La-5.5] was similar between trained groups and higher than SC. PET presented higher values for glucose metabolism than CT and SC. These results open up new perspectives for studying training methods used in high performance sport through swimming exercise in rats.

Efeitos de uma pré temporada em parâmetros anaeróbios e comparação de parâmetros aeróbios e anaeróbios de futebolistas de diferentes categorias

Pós-graduação em Fisioterapia - FCT

Coordenação da ação dos braços no nado crawl analisada em diferentes intensidades, nos exercícios contínuo e intermitente

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Respostas fisiológicas durante o exercício intervalado realizado no ciclismo com recuperação passiva e ativa

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Validade do teste do lactato mínimo para a determinação do limiar anaeróbio em ratos durante exercício de natação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Respostas metabólicas e da técnica de nado durante o exercício realizado na velocidade correspondente à máxima fase estável de lactato sanguíneo determinada de forma contínua e intermitente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Validade do modelo distância-tempo para a determinação das capacidades aeróbia e anaeróbia em jogadores de futebol

Pós-graduação em Ciências da Motricidade - IBRC

Análise de parâmetros de força e padrão de nado de ratos controles e obesos, a partir de um sistema de aquisição de sinais em natação atada: Influência da intensidade do exercício e da densidade corporal

Pós-graduação em Ciências da Motricidade - IBRC

Protocolos de treinamento aeróbio intervalado e da periodização para natação com ratos

Pós-graduação em Ciências da Motricidade - IBRC

Efeito do nível do treinamento aeróbio na determinação do limite superior do domínio pesado no ciclismo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Protocolos invasivos e não invasivos para avaliação aeróbia e anaeróbia de ratos wistar

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da duração do estímulo e do tipo de recuperação nas respostas metabólicas e cardiorrespiratórias durante um exercício intermitente realizado no domínio severo em indivíduos ativos

Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC

Concentração de lactato sérico em ovelhas e cordeiros mestiços (1/2 Suffolk) nascidos em eutocia

The aim of this study was to evaluate the venous blood lactate concentration in sheep and newborn lambs immediately after normal delivery. We used 14 ewes, nine and four Suffolk crossbred lambs and 20 healthy newborns (males and females). The effect of gender, race and number of lambs per birth was investigated, as well as the possible correlation between the concentration of maternal and newborn lactate. There was no difference between males and females or in relation to race (and Suffolk crossbred); however the concentration of lactate was higher in twin or triplet lambs when compared to single lambs. There was a positive correlation between the maternal and newborn concentration and the higher the concentration of lactate, the greater the concentration in the lamb immediately after normal delivery. The results of this study provide a base from which to compare the blood lactate levels in lambs in critical condition.

Reprodutibilidade das respostas metabólicas e cardiorrespiratórias fisiológicas em um protocolo de exercício intermitente realizado com recuperação passiva e ativa

The main objective of this study was to analyze the reliability of blood lactate concentration ([La]), oxygen uptake (VO2) and heart rate (FC) in an intermittent protoco, performed at 95%VO2max with passive or active recovery in untrained subjects. Participated of this study, active healthy males with 20 to 25 years, which were doing aerobic exercises witha weekly frequency of 3 sessions at least. The individulas performed, in different days, the following protocols in a cyclergometer: 1) An incremental test until exhaustion to determine maximal oxygen uptake (VO2max) and the intensity at VO2max; b) Two transitions at 95%VO2max for the determination of the VO2 kinetics parameters and; c) Two intermittent tests until exhaustion, with repetitions at 95% IVO2max and with durantion defined as being half of the duration of the slow component. The duration of the recovery was half of the duration of the effort (effort:pause of 2:1). This test was performed with passive (GP) and active recovery (GA). The VO2 and FC were measured continulously in both tests. Blood collections were performed for the determination of the [La]. There was significant correlação in both groups for VO2 (ATIVA - 0.94, PASSIVA - 0.75), [La] (ATIVA - 0.83, PASSIVA - 0.90) and FC (0.93) only for the passive group. Thus, it can be concluded that the cardiorrespiratory and metabolic responses present good realiability in an intermittent exercise with active or passive recovery