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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Digital image analysis of root distribution towards improved irrigation water and soil management: Grapevine and date palm study cases

It is presented two study cases about the approach in root analysis at field and laboratory conditions based on digital image analysis. Grapevine (Vitis vinifera L.) and date palm (Phoenix dactylifera L.) root systems were analyzed by both the monolith and trench wall method aided by digital image analysis. Correlation between root parameters and their fractional distribution over the soil profile were obtained, as well as the root diameter estimation. Results have shown the feasibility of digital image analysis for evaluation of root distribution.

Avaliação de seis variedades de uvas sem sementes no Submédio São Francisco

This work aimed to study aspects related to vegetative and productive development of plants and characteristics and chemical components of bunches of six seedless grape varieties at environmental conditions of São Francisco river's valley, Northeastern Brazil. The experiment was carried out during 1997 and 1998. The varieties tested were grafted on IAC 572 ('Jales') rootstock. The experimental model utilized was totally randomized, and with parcels subdivided into different production cycles. The results showed significative differences among varieties at different pruning times. The average weight of bunches varied from 164.8 g for 'Marroo Seedless' and 203.5 g for 'Beauty Seedless'. The average diameter of berries was superior to 15.7 mm for all of the varieties. The total soluble solids varied from 14.05°Brix in 'Canner' to 19.6°Brix in 'Venus'. The total titratable acidity (ATT) was lower than 0.91 g tartaric acid/100 mL of juice. The ratio SST/ATT varied from 19.05 in 'Beauty Seedless' to 28.57 in 'Vênus'. 'Vênus' and 'Marroo Seedless' were the most productive varieties, with the average annual production of 24 ton/ha and 20 ton/ha, respectively.

Influência de Manejos de irrigação sobre aspectos de Ecofisiologia e de produção da videira cv. Syrah / Paulsen 1103

O manejo de irrigação pode influenciar o comportamento ecofisiológico e a produção da videira. O objetivo desse trabalho, conduzido em 2010 em Petrolina – PE, no Submédio do Vale do São Francisco, foi avaliar a influência de diferentes estratégias de manejo de irrigação no potencial de água na folha e em aspectos qualitativos e quantitativos das uvas do primeiro ciclo de produção da videira cv. Syrah/Paulsen 1103. O sistema de irrigação utilizado foi gotejamento e a lâmina de água foi estimada com base na evapotranspiração da cultura. O delineamento experimental utilizado foi o de blocos casualizados, em 4 repetições e com 3 tratamentos: irrigação plena, realizada durante todo o ciclo de produção; a irrigação com déficit, onde a aplicação de água foi interrompida na fase fenológica de cacho fechado; e a irrigação com déficit controlado, onde a irrigação, também interrompida na fase de cacho fechado, foi eventualmente realizada após a interrupção, de acordo com o monitoramento da água no solo. A imposição de déficit hídrico às plantas favoreceu uma maior concentração de açúcares e a redução da acidez nos frutos, contribuindo para a melhoria da qualidade das uvas para vinificação.

Wine aroma improvement using a β-glucosidase preparation from aureobasidium pullulans

Microbial β-glucosidases have been used for the enhancement of wine aroma. Nevertheless, few enzymes are active in the conditions of winemaking. In this work, the production of a β-glucosidase by an Aureobasidium pullulans strain (Ap-β-gl) isolated from grape ecosystems was evaluated. The maximum enzymatic synthesis using submerged fermentation was after 96 h of growth in complex media containing 20 g/L of cellobiose as the sole carbon source. The crude enzyme (Ap-β-gl) showed optimal pH at 5.5 and two peaks of optimum temperature (at 45 and 70 C). It showed a wide range of pH stability, stability at low temperatures, and tolerance to ethanol, showing suitable characteristics for winemaking conditions. The hydrolysis of glycosidic terpenes by Ap-β-gl was studied, and its ability to efficiently release free terpenols was demonstrated by gas chromatography/mass spectrometry. The enzymatic treatment notably increased the amount of monoterpenes, showing good prospects for its potential application for the development of aroma in wines. © 2012 Springer Science+Business Media New York.

Purification and Characterization of an Ethanol-Tolerant β-Glucosidase from Sporidiobolus pararoseus and Its Potential for Hydrolysis of Wine Aroma Precursors

An extracellular ethanol-tolerant β-glucosidase from Sporidiobolus pararoseus was purified to homogeneity and characterized, and its potential use for the enhancement of wine aroma was investigated. The crude enzymatic extract was purified in four steps (concentration, dialysis, ultrafiltration, and chromatography) with a yield of around 40 % for total activity. The purified enzyme (designated Sp-βgl-P) showed a specific activity of approximately 20.0 U/mg, an estimated molecular mass of 63 kDa after sodium dodecyl sulfate polyacrylamide gel electrophoresis, and isoelectric point of 5.0 by isoelectric focusing. Sp-βgl-P has optimal activity at pH 4.0 and at 55 °C. It was stable in a broad pH range at low temperatures and it was tolerant to ethanol and glucose, indicating suitable properties for winemaking. The hydrolysis of glycosidic terpenes was analyzed by adding Sp-βgl-P directly to the wines. The released terpene compounds were evaluated by gas chromatography/mass spectrometry. The enzymatic treatment significantly increased the amount of free terpenes, suggesting that this enzyme could potentially be applicable in wine aroma improvement. © 2013 Springer Science+Business Media New York.

Phenolic composition of the berry parts of hybrid grape cultivar BRS Violeta (BRS Rubea×IAC 1398-21) using HPLC-DAD-ESI-MS/MS

The grape is considered a major source of phenolic compounds when compared to other fruits and vegetables, however, there are many cultivars with distinct characteristics directly linked to phenolic profile. Thus, the present study aimed to identify and quantify, for the first time and in detail, the phenolic compounds present in the skin, flesh and seeds of BRS Violeta grape berry using combination of SPE methodologies and analytical HPLC-DAD-ESI-MS/MS. The study was extended to the different berry parts and the most important grape and wine phenolic families, and has revealed interesting features. Violeta grape has a very thick skin (46% of grape weight) that accumulated the most of grape phenolic compounds: great amount of anthocyanins (3930. mg/kg, as malvidin 3,5-diglucoside), together with also important amounts of flavonols (150. mg/kg, as quercetin 3-glucoside), hydroxycinnamic acid derivatives (HCAD; 120. mg/kg, as caftaric acid), and proanthocyanidins (670. mg/kg, as (+)-catechin); in contrast, it seems to be a low resveratrol producer. Violeta grape seeds accounted for similar proportions of low molecular weight flavan-3-ols (mainly monomers; 345. mg/kg, as (+)-catechin) and proanthocyanidins (480. mg/kg, as (+)-catechin). Violeta grape is a teinturier cultivar, but it only contained traces of anthocyanins and low amounts of all the other phenolic types in its red-colored flesh. The anthocyanin composition of Violeta grape was dominated by anthocyanidin 3,5-diglucosides (90%). Within flavonols, myricetin-type predominated and kaempferol-type was missing. In addition to expected hydroxycinnamoyl-tartaric acids, several isomeric esters of caffeic and p-coumaric acids with hexoses were tentatively identified, accounting for relevant proportions within the pool of HCAD. Although pending of further confirmation over successive vintages, the aforementioned results suggest that BRS Violeta grape cultivar could be considered an interesting candidate for the elaboration of highly colored and antioxidant-rich grape juices and wines. © 2013 Elsevier Ltd.

Comportamento fermentativo de linhagens industriais de Saccharomyces cerevisiae em mosto com diferentes concentrações de sacarose e fontes estruturalmente complexas de nitrogênio

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Bidestilação em alambiques contendo dispositivos de prata e cobre e sua influência na qualidade da cachaça

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento e caracterização de vinhos tintos a partir de uvas cultivadas no noroeste paulista

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Rastreamento de leveduras autóctonas para a produção de pectinase, tanase e invertase

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Reologia de vinhos tintos secos produzidos no Sul do Brasil

Pós-graduação em Engenharia e Ciência de Alimentos - IBILCE

Bioecologia de Anastrepha fraterculus (Wiedemann, 1830) (Diptera: Tephritidae) em videira

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Danos causados por Mahanarva fimbriolata (STAL, 1854) na qualidade da cana e processo fermentativo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Imobilização multipontual covalente de xilanases: seleção de derivados ativos e estabilizados

Pós-graduação em Biotecnologia - IQ