66 resultados para Virus diseases of plants
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Extracts of plants of the Sclerolobium genus are disclosed, as well as methods for preparing same and cosmetic and pharmaceutical formulations containing these extracts. These extracts can be obtained by polar and/or apolar extraction, each fraction containing different anti-oxidants, all of which are useful for topical treatment. Also disclosed is the use of this extract in cosmetic and/or pharmaceutical formulations for topical use.
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Rapid tests to assess the nutritional status of plants gerbera can make easier the decision of any adjustments in the fertilization of the substrate. The study was conducted to evaluate nutritionally fertigated gerbera plants with increasing levels of electrical conductivity (EC), using portable meters nitrate and potassium, and chlorophyll. For this, two experiments were conducted. A randomized block design with five levels of EC (0.5, 2.0, 3.5, 5.0 and 6.5 dS m-1) and four replications was used. Cherry cultivar was used in the first experiment. In the second experiment, two cultivars (Cherry and Salmon Rose) were used. In the first experiment, the sap of the leaves was subjected to rapid testing of N-NO3- (equipment Cardy Horiba C -141). N-NO3- and K+ (C-131) were determined in the substrate solution. The intensity of the green leaf, in the second experiment was evaluated with portable Chlorophyll Meter. The N content in the indicator leaf in the first and N and K contents in the plant in the second experiment were determined. Rapid tests are presented as good indicators of the level of N-NO3- and K+ in plant tissue, with the advantages of convenience and speed of determination, facilitating monitoring of fertilization of the substrate by producers and technicians.
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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The use of plants compounds for the control of insects has increased worldwide. This occurs because the vegetal insecticides contains biodegradable compounds, nontoxic products and potentially suitable for use in pest control. Plants of the family Annonaceae are standing out as biopesticides because they are bioactive naturally in addition to presenting cytotoxic activity, antitumor, vermifuge, antimicrobial, immunosuppressive, anti-emetic, inhibiting appetite, antimalarial and also insecticide. The insecticidal activity of Annonaceae is due to the presence of acetogenins, substances that act on mitochondria inhibiting the NADH -ubiquinone oxidoreductase, causing the death of insects. In this review we report the use of Annonaceae in insect control, showing that so far, only 42 species of Annonaceae have information insecticidal activity against just over 60 species of insect pests. This information shows that much research is still needed, especially to get to know the insecticidal activity of other Annonaceae species, in addition to its effects on insect pests not yet studied. So we will have as an alternative to sustainable development, new vegetal insecticides such as those obtained from different Annonaceae species, which can act as an additional tool to balance the excesses of agriculture chemical or conventional.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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A peptide-based indirect ELISA was developed to detect antibodies against Equine arteritis virus (EAV). Two peptides for epitope C of protein GP5 and fragment E of protein M were designed, synthesized, purified and used as antigens either alone or combined. Ninety-two serum samples obtained from the 2010 Equine viral arteritis outbreak, analyzed previously by virus neutralization, were evaluated by the ELISA here developed. The best resolution was obtained using peptide GP5. The analysis of the inter- and intraplate variability showed that the assay was robust. The results allow concluding that this peptide-based ELISA is a good alternative to the OIE-prescribed virus neutralization test because it can be standardized between laboratories, can serve as rapid screening, can improve the speed of diagnosis of EAV-negative horses and can be particularly useful for routine surveillance in large populations. (C) 2014 Elsevier B.V. All rights reserved.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aprefeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Metagenomics was used to characterize viral genomes in clinical specimens of horses with various organ-specific diseases of unknown aetiology. A novel parvovirus as well as a previously described hepacivirus closely related to human hepatitis C virus and equid herpesvirus 2 were identified in the cerebrospinal fluid of horses with neurological signs. Four co-infecting picobirnaviruses, including an unusual genome with fused RNA segments, and a divergent anellovirus were found in the plasma of two febrile horses. A novel cyclovirus genome was characterized from the nasal secretion of another febrile animal. Lastly, a small circular DNA genome with a Rep gene, from a virus we called kirkovirus, was identified in the liver and spleen of a horse with fatal idiopathic hepatopathy. This study expands the number of viruses found in horses, and characterizes their genomes to assist future epidemiological studies of their transmission and potential association with various equine diseases.