Evaluation of the interactions of DNA with the textile dyes Disperse Orange 1 and Disperse Red 1 and their electrolysis products using an electrochemical biosensor

A disposable pencil graphite electrode modified with dsDNA was used in combination with square wave voltammetry in order to evaluate the interaction of DNA with the textile dyes Disperse Orange 1 (DO1) and Disperse Red 1 (DR1), and with the products of their electrolysis. Significant changes in the characteristic oxidation peaks of the guanine and adenine moieties of immobilized dsDNA were observed after incubation of the modified electrode for 180 s in solutions of the dyes in their original forms. The same was observed using the electrolysis products obtained by oxidation and reduction conversions. The oxidation peak currents of the guanine and adenine moieties decreased when the concentrations of DO1 and DR1 were increased up to 5.0 × 10 -6 and 1.0 × 10-6 mol L-1, respectively; the signal decreases were more pronounced after interaction with the oxidized dyes, compared to the reduced compounds. The interactions between DNA and DO1, DR1, and the electrolyzed dyes were further investigated by UV-vis spectrophotometry in solution, and different effects such as hypochromism and hyperchromism were observed in the resulting DNA spectra. The investigated interactions showed clear evidence of changes in the DNA structure, and suggested a predominant intercalation mode leading to damage in the biomolecule. © 2013 Elsevier B.V.

Effect finishing and polishing procedures on the surface roughness of IPS Empress 2 ceramic

Objective. To evaluate the surface roughness of IPS Empress 2 ceramic when treated with different finishing/polishing protocols. Materials and methods. Sixteen specimens of IPS Empress 2 ceramic were made from wax patterns obtained using a stainless steel split mold. The specimens were glazed (Stage 0-S0, control) and divided into two groups. The specimens in Group 1 (G1) were finished/polished with a KG Sorensen diamond point (S1), followed by KG Sorensen siliconized points (S2) and final polishing with diamond polish paste (S3). In Group 2 (G2), the specimens were finished/polished using a Shofu diamond point (S1), as well as Shofu siliconized points (S2) and final polishing was performed using Porcelize paste (S3). After glazing (S0) and following each polishing procedure (S1, S2 or S3), the surface roughness was measured using TALYSURF Series 2. The average surface roughness results were analyzed using ANOVA followed by Tukey post-hoc tests (α = 0.01) Results. All of the polishing procedures yielded higher surface roughness values when compared to the control group (S0). S3 yielded lower surface roughness values when compared to S1 and S2. Conclusions. The proposed treatments negatively affected the surface roughness of the glazed IPS Empress 2 ceramic. © 2013 Informa Healthcare.

Transcript finishing initiative: contribuição do laboratório IL2

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Electrochemical investigations on the capacity of flavonoids to protect DNA against damage caused by textile disperse dyes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Passion fruit seed meal at growing and finishing pig (30-90 kg) feeding

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Citrus pulp and enzyme complex for growing and finishing pigs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Impact of the Textile Dye Acid Blue 40 on the Periphyton of a Simulated Microecosystem

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bioremediation of direct dyes in simulated textile effluents by paramorphogenic form of Aspergillus oryzae

Azo dyes are extensively used for coloring textiles, paper, food, leather, drink, pharmaceutical products, cosmetics and inks. The textile industry consumes the largest amount of azo dyes, and it is estimated that approximately 10 - 15% of dyes used for coloring textiles might be lost in waste streams. Almost all azo dyes are synthetic and resist biodegradation, however, they can be readly reduced by a number of chemical and biological reducing systems. Biological treatment is advantageous over physical and chemical method as result of its low cost and little disturbance to the environment. This research focuses on the utilization of Aspergillus oryzae, to remove some kinds of azo dyes from aqueous solutions. The fungi, physically induced in its paramorphogenic form (called, pellets), were used in the dyes biosorption studies with both non autoclave and autoclaved hyphas, at differents pH values. Thus the goals are the removal of dyes by biosorption and the decrease of its toxicity.

Finishing precocity visual score and genetic associations with growth traits in Angus beef cattle

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Color stability of a nanofilled resin: influence of polishing and finishing and fluoride solutions according to time

Aim: The aim of the study was evaluate the finishing and polishing effect of the color stability of the composite resin Filtek Supreme XT, according to different fluoride solutions and time. Material and Methods: Specimens were prepared (n=140) with half of the samples finished and polished. The experimental groups were divided according to the presence or absence of finishing and polishing and immersion solutions (artificial saliva, sodium fluoride solution at 0.05% - manipulated, Fluordent Reach, Oral B, Fluorgard). The specimens remained in artificial saliva for 24 hours and were subjected to an initial color analysis using a spectrophotometer CIELab system. Then, they were immersed in the experimental solutions for 1 minute a day. The readings of the color change were made after 24 and 48 hours, 7, 14, 21, 30 and 60 days after the first immersion. The three-way mixed Analysis of Variance (ANOVA) ("finishing/polishing", "immersion medium" and “time”) were performed. For multiple comparisons, the Sidak test for repeated measure was used, with a 5% level of significance. Results: The finishing and polishing factor showed significant variability, independently of the immersion media (p<0.001). Cconclusion: Finishing and polishing procedures yielded better color stability to composite resin over time, regardless of the immersion media.

Chemical and morphological features of nanofilled composite resin: influence of finishing and polishing procedures and fluoride solutions

This study evaluated the influence of finishing and polishing procedures and differ-ent fluoride solutions on superficial morphology and chemistry of the nanofilled composite resinSupreme XT (3M) through the EDX analysis and SEM evaluation. Circular specimens (n 5 30) of 10mm diameter and 2 mm thickness were prepared, with half of the sample assays finished and polishedwith Super-Snap1sandpaper. The experimental groups were divided according to the presence or ab-sence of finishing and polishing and solutions (arti ficial saliva, 0.0 5% of manipulated sodium fluoridesolution, Fluordent Re ach, Oral B, Fluorgard). Specimens were immersed in each respective solutionfor 1 min per day, during 60 days and stored in artificial saliva at 37 6 18C between immersion peri-ods. Topography and chemical analysis was qualitative. It was observed that specimens submitted tofinishing and polishing procedures had lower superficial degradation. Fluoride solutio ns promoted su-perficial alterations on specimens, being the highest degradation obtained with Fluordent Reach. Itca n be concluded that finishing and polishing procedures and the immersion media influence the su-perficial morphology of composite resin tested; the Fluordent Reach was the flu oride solution thatmo st affected the material’s surface.

Effect of finishing and polishing on color stability of a nanofilled resin immersed in different media

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Disperse Red 1 (textile dye) induces cytotoxic and genotoxic effects in mouse germ cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)