Caracterização físico-química e avaliação sensorial de queijo tipo boursin de leite de cabras alimentadas com raspa de mandioca

Pós-graduação em Zootecnia - FMVZ

Epidemiologia molecular aplicada ao monitoramento de estirpes de Staphylococcus aureus na produção de queijo Minas frescal

We studied the molecular epidemiology of Staphylococcus aureus strains potentially toxigenic, isolated from the production process of Minas frescal cheese in a small dairy plant in the state of São Paulo. For this, samples were taken during the period from June 2008 to July 2009. Samples were collected from the surface of the receiving and storage tanks of raw milk, the surface of the balance tank of pasteurized milk, the water supply system, the pipes and equipments, the hands of the handler and from the packaged cheese, totaling 140 samples. The colonies isolated on Baird-Parker Agar confirmed as Gram positive and positive for catalase, coagulase and acetoin production, were submitted to extraction of bacterial DNA using the Invitek - Uniscience® kit. Confirmation of the isolated species and enterotoxins SEA, SEB, SEC, SED and TSST-1 toxin was carried out through the amplification of specific fragments of chromosomal DNA. Among the 74 strains of isolated coagulase-positive staphylococci, only 41 (55.4%) strains were confirmed as Staphylococcus aureus, of which 25 (61.0%) were positive to the presence of staphylococcal toxins. The most frequently identified enterotoxin was SEA. The toxigenic strains of Staphylococcus aureus were more frequently isolated from hands of the handler (16.0%), raw milk receiving tank (12.0%), pasteurized milk for cheese making (12.0%) and fresh white cheese ready for consumption (12.0%).

Caracterização do leite e do queijo de ovelhas da raça bergamácia suplementadas com óleo ou farelo de linhaça (Linum usitassimum L.)

Pós-graduação em Zootecnia - FMVZ

Propriedades tensiométricas do peritônio da paca (Cuniculus paca) a fresco e conservado em glicerina 98%

In constant searching for alternative biological material to perform implants and new options of experimental animal models, the objective of this investigation was to describe the mechanical properties of the peritoneum paca (Cuniculus paca Linnaeus, 1766) fresh and preserved in 98% glycerin. Samples of fresh and preserved in glycerin for periods of 30, 60 and 90 days were subjected to mechanical tests. Four adult animals, male or female, with mean body weight of eight kilograms, were used for collecting samples of the peritoneum. All tissues preserved in glycerin 98% showed a decrease in stiffness and increase in ductility and toughness. Considering the maximum force applied to the peritoneum, significant increase was observed in values (p<0.01) of samples stored for 60 and 90 days when compared to fresh material. In relation to the stretch variable, an increase was observed in all storage time of glycerin samples, verifying significant difference (p<0.01) when compared with the fresh samples. The variable area also showed significance (p <0.01) between the values of the fresh samples (5.40 mm(2)) and preserved in the glycerin by periods of 30 days (4.50 mm(2)), 60 days (9.00 mm(2)) and 90 days (7.20 mm(2)), thus indicating that the area of this membrane increased by 0.033 mm(2) per day. Generally, it was concluded that the 98% glycerin is a substance effective for the preservation of the peritoneum of the agouti paca, therefore improves its mechanical properties allowing the support membranes greater deformation forces. Thus, the results obtained in mechanical tests of the peritoneum of paca suggest its use as an alternative biological material.

Biodisponibilidade de flavanonas e atividade antioxidante do suco de laranja fresco versus suco de laranja pasteurizado em humanos saudáveis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características físico-químicas e sensoriais de embutido fresco de aves de descarte preparado com diferentes antioxidantes naturais

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação de qualidade de amidos de mandioca modificados utilizados na fabricação de pão de queijo

The food industry looks for new ways and strategies to standardize the marketing products in the customer market. The modification of the cornflour that are produced in the manufacturing of the cheese bread has been one of the these ways, since some raw materials that are used to make the cornflour present different features in each supplyed allotment. The present research had as a main purpose to analyse such features looking forward to improving the quality of the final product. Thus, some tests were done to verify the pH, humidity and acidity; it was checked the percentage of carbonyl and carboxylic groups and the power of expansion to five different allotments and to farina, for example: pre-gel, natural, expandex farina as the combination of the three cited kinds. After diversifying the allotments, different results were acquired, even to an unique kind of farina and it was not possible to correlate the results of acidity, the percentage of carbonyl and carboxylic groups and the power of expansion. The pH and humidity are proper for each kind of farina. This way, the raw materials that are used for each cheese bread manufacturing must be analysed in each allotment

Inseminação artificial com sêmen fresco e sua aplicabilidade como biotecnologia da reprodução na espécie ovina

With the increasing development of the brazilian sheep production, the producer was forced to achieve higher production rates. The use of artificial insemination has been shown as an important biotechnological tool in animal breeding. Among the various existing techniques, the superficial cervical insemination with fresh semen is demonstrating fertility rates between 70 and 80% (AISEN, 2008), in addition to its applicability, it does not require sophisticated equipment or manpower to highly specialized implementing these plans greater possibility in your job to maximize reproduction and greater dissemination of superior genetic material on the property. This study aims to address aspects of artificial insemination with fresh semen in sheep and its applicability in commercial herds biotechnology as a tool in assisted reproduction

Resposta endometrial de jumentas (Equus asinus) da raça Pêga ao sêmen fresco, extendido e congelado de jumento

Pós-graduação em Cirurgia Veterinária - FCAV

Queijo Prato com teor reduzido de gordura adicionado de enzima proteolítica: características físicas e sensoriais

The Prato cheese is the second most consumed cheese in Brazil. It is produced by milk enzymatic coagulation, and maturated for at least 25 days; it is classified as fatty cheese and of medium moisture. Due to the concern to health, the cheeses consumers have been seeking for products with low fat contents; however fat is essential for providing desirable sensory and physiologic characteristics, such as flavor, softness and texture to cheeses. Alterations on the technological processing of low fat cheeses have been made seeking for improved products, and the use of proteolytic enzymes has been a significant strategy. The meltability, color and sensory characteristics are fundamental quality indicators of the final products. This study reports the findings from the analyses on the physical and sensory characteristics of low fat Prato cheese with addition of proteolytic enzyme – fastuosain, that is extracted from unripe gravata fruit. The addition of fastuosain improved the quality of the product, as this additive neither affected the meltability, nor produced bitterness, which is a common unpleasant taste in low fat cheeses.

Capacidade antioxidante do suco de laranja fresco versus pasteurizado no sangue de indivíduos adultos

O suco de laranja é um alimento rico em substâncias antioxidantes, como os flavonoides cítricos, vitamina C e carotenoides, que podem reduzir o estresse oxidativo que está relacionado à gênese das doenças cardiovasculares, diabetes e câncer. No entanto, o processamento da laranja para obtenção do suco interfere no tipo e quantidade dos compostos antioxidantes, podendo comprometer as ações esperadas. O objetivo deste trabalho foi avaliar a atividade antioxidante do suco de laranja, comparando os resultados após a ingestão aguda do suco de laranja fresco e do suco de laranja pasteurizado comercialmente em indivíduos adultos saudáveis. Cada voluntário foi submetido a dois tratamentos: ingestão aguda de suco de laranja fresco e, após intervalo de 30 dias, ingestão aguda de suco de laranja pasteurizado. Nos dois tratamentos foram colhidas amostras de sangue dos indivíduos antes e após a ingestão do suco de laranja. O efeito antioxidante total foi mensurado pelo ensaio do radical ABTS+ (2,2'azinobis (3-etilbenzenotiazolina 6-sulfônico) e pelo ensaio de TBARS (substâncias reativas ao ácido tiobarbitúrico) nas amostras de sangue. Observou-se um aumento de 2,2% na capacidade antioxidante do sangue a partir de 4 horas após a ingestão do suco de laranja fresco e que se manteve estável até 24 horas do consumo (p<0.05). Já com a ingestão do suco de laranja pasteurizado, a capacidade antioxidante se manteve estável e equivalente ao suco de laranja fresco nas primeiras 4 horas, mas aumentou 1,4% após 8 horas, permanecendo neste nível até as 24 horas seguintes (p<0.05). Após a ingestão do suco fresco, os níveis de peroxidação lipídica, diminuíram 27% nas primeiras 4 horas. Porém, a partir das 8 h retornaram ao valor inicial. Os resultados após o consumo do suco pasteurizado mostraram uma redução da peroxidação lipídica em 18% a partir das 4 horas e 8 h (p<0.05), e esta redução se acentuou (27%) às 24 h ...

Utilização de melaço, manipueira e soro de queijo como substratos para produção de biossurfactante por Bacillus Pumilus e sua aplicação em biorremediação de solo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desenvolvimento de filmes biodegradáveis à base de zeína, caracterização das propriedades funcionais e estruturais e avaliação do uso como cobertura na conservação das características físico-químicas do queijo Minas padrão

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diversidade e evolução da microbiota lática autóctone em queijo Muçarela de búfala e aplicação tecnológica dos isolados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Rendimento e qualidade do queijo tipo Minas Frescal de cabras alimentadas com raspa de mandioca e alfalfa

Pós-graduação em Zootecnia - FMVZ