Estado físico do meio de cultura na propagação in vitro de bromeliaceae

The objective of this study was to evaluate the effect of the physical state of the culture medium on the behavior in vitro of the bromeliaceas Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incun/ata. Micropropagated shoots had been cultivated in culture medium MS, with 30 g sucrose, 2,5 mg dm-3 BAP, and 0,5 mg dm-3 ANA, establishing the treatments: T1- half-solid with 7 g agar; T2- static liquid; T3- liquid under-agitation of 90 rpm; and T4-static liquid with bridge of paper filter. After 30 days, the use of static way of liquid culture presented better results in relation of proliferative average rate in all the species (9.4 shoots in N. cruenta, 5.6 in T. stricta, 11.5 in V. gigantea, 9.2 in V. guttata and 3.9 in V. incurvata). In relation the average height of the shoots, was distinguished for N. cruenta the liquid under-agitation (2.83 cm), T. stricta and V. incurvata the semisolid (1.76 cm and 2.02 cm, respectively) and V. gigantea and V. guttata the static liquid (0.61 cm and 1.48 cm, respectively). The use of medium MS static liquid was more suitable for in vitro culture of bromeliads species studied.

Avaliação histológica e morfométrica do retalho axial oris angularis e da terapia por ondas de choque aplicados a defeito palpebral experimental em cães

Considering that the cutaneous flap can be affected by isquemic complications the extra corporeal shock wave therapy (ESWT) was described as rescue techniques. The present study was developed to analyze histological and with morfometry, twenty one skin samples treated or not with the shock wave therapy, obtained from flap's distal border, used in this study to repair eyelids' experimental defects in dogs. The flap with or without ESWT did not show any histological sign of inflammatory or atrophic alterations. Both group treated showed similar morphometrical characteristics. The ESWT with the protocol used in this study (2500 impulses at 0,15 mJ/mm 2) did not demonstrate significant clinical outcomes as a rescue technique when applied over the oris angularis flap, however results showed no signals of collateral deleterious effects.

Informative Radio Models in Brazil: The All-News Networks

This study analyzes the particularities of Brazilian radio networks that adopt the all-news format and briefly presents the main national and international experiences for the implementation of the model and its conceptualization. Besides the bibliographic review, we use the multiple-case study, analyzing as the empirical objects CBN and BandNews FM networks. Also, we apply methodological procedures of systematic non-participant observation, supplemented by interviews and surveys. We conclude that the different all-news programming models and network organizations influence the processes of production, information structure, broadcasting language, and therefore the stations' profile. © 2011 Copyright Taylor and Francis Group, LLC.

Influência da posição e tempo de irradiação sobre a capacidade de desinfecção em forno de micro-ondas

Objective: To evaluate the variations of temperature in 2 models of domestic micro-wave ovens, single emission (F 1) and dual emission of waves (F 2), to investigate areas of higher and lower intensity of the electromagnetic field. Materials and methods: A beaker containing water (60mL, 26°C) was irradiated into each of 5 positions (front - P 1; right - P 2; posterior - P 3; left - P 4; central - P 5) within each oven (900W/ 2min). To evaluate the effectiveness of disinfection in F 2, Bacillus subtilis suspension was irradiated in each of the 5 positions for 2, 4 and 6minutes. Data were analyzed by Kruskal-Wallis and nonparametric multiple comparisons at 5% significance level. Results: 84.80°C (F 1) and 92.01°C (F 2) were mean levels of temperature. For F 1, the positions P 1, P 2, P 3 and P 5 showed similar values among them and upper than P 4, while for F 2, the positions P 1, P 2 and P 4 were similar among them and upper than P 3 and P 5. Kruskal-Wallis test found significant differences between positions and models of ovens (p<0.05). It was observed that P 2 promoted bacterial death from 4min of irradiation, while the other positions promoted disinfection at 6min of irradiation. Conclusion: The protocols of position and time specified for the various procedures in microwave ovens can be different according to the characteristics of each device due to the electromagnetic field heterogeneity. © 2011 Sociedade Portuguesa de Estomatologia e Medicina Dentária.

Propagação assexuada de chacrona (psychotria viridis ruiz & pavon) via estaquia foliar

This study aimed to assess the asexual propagation of Psychotria viridis by leaf cuttings. The treatments were: A - Whole leaf cuttings, B - leaf cuttings with the top third cut off; C - with the lower third of the cuttings removed; D - with cuts on the primary vein of leaf cuttings, and E - poles with cuts on the leaf's secondary veins. The cuttings were immersed in distilled water for a period of 70 days. In all the treatments, the rooting was observed to occur in the region of the cut, or the place where the incision in the vein was. Current assay shows the feasibilities of Psychotria viridis leaf stalks and concludes that cuttings at the nerve ends highlight rooting in so far as the leaf stalks remain in permanent contact with the plant.

Determinação da densidade mineral e da elasticidade óssea após a aplicação de ondas de choque extracorpóreas no terceiro metacarpiano de equinos atletas

The porpoise of this study was to evaluate the effects of extracorporeal shock waves in third metacarpus bone from healthy horses by determination of bone elasticity. It were used 20 Thoroughbred horses, male and female, with two years old, on top of training and selected as the state healthy. At the beginning of the experiment (D0), all animals were submitted for evaluation of bone elasticity held in the third metacarpus bone. The animals were divided into two groups (Control Group - CG and Experimental Group - EG). The application of extracorporeal shock wave therapy (ESWT) was performed on the right forelimb of the animals in the experimental group in the same place evaluated for bone elasticity and was used apparatus for extracorporeal therapy of waves with 0.15 mJ/mm2 energy flux density and 2000 pulses with E6R20 probe, with focus feature of the shock wave of 20 mm. The ESWT were repeated every 21 days, a total of three sessions (D0, D21 and D42). The analysis of bone elasticity determination was realized at D21, D42 and D72. The average of speed ultrasound differed between groups at D21, D42 and D72, and the animals from EG had lower bone mineral density after applications of ESWT. There was also difference in the analysis of bone mass (Z-Score) between the groups at D21 and D42, which animals from EG showed a significant decrease in bone mass. The risk of fracture was higher in animals from experimental group at D21. It was concluded that ESWT is able to promote change in bone mineral density.

Utilização de reguladores vegetais na propagação vegetativa de clidemia blepharodes DC. e C. Suffruticosa O. Berg (Melastomataceae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Propagação in vitro de Cattleya trianaei (Linden & Reichenbach fil.) (Orchidaceae) em meios de culturas e com doses de fertilizante comercial

The use of culture media produced with commercial fertilizers can represent a simple and low cost alternative for commercial orchid propagation. The aim of this study was to evaluate the in vitro growth of plantlets of Cattleya trianaei in culture medium MS reduced and and formulated with Peters® NPK 10-30-20 in different doses. 90 day-old plantlets with two leaflets were submitted to five treatments (T1 - reduced MS; T2 - Peters® 1 g L -1; T3 - Peters® 2 g L-1; T4 - Peters® 3 g L -1 and T5 - Peters® 5 g L-1) arranged in a completely randomized design with five replicates with 25 plantlets for each treatment and incubated during 180 days, with subcultures at each 60 days, when the number of roots, root length, number of leaves, shoot length and shoot fresh matter were evaluated. The simplified culture medium with fertilizer Peters® 3 g L -1 presented results statistically different as for the number of roots, number of leaves, shoot length and shoot fresh matter and it can be recommended for in vitro growth of this ornamental orchid.

Seleção de agentes alternativos ao ágar para propagação de plântulas de Cattleya loddigesii Lindley (Orchidaceae)

The aim of this study was to evaluate the in vitro growth of Cattleya loddigesii in alternative agents to agar with starch and physical matrix with acclimatization of regenerated plants. Protocorms with 90 days after sowing (0.5 cm of length) were subcultured in 1/2 MS culture medium among the treatments consisting of: agar 7 g L-1 (T1, which corresponds the control), agar 3,5 g L-1 with cassava starch 30 g L-1 (T2), cassava starch 60 g L-1 (T3), cotton fiber (T4) and chopped polyurethane foam (T5). Plantlets were retained in these treatments for over 150 days, and at the end of in vitro culture, were analyzed by their biometric data and acclimatized in a greenhouse during 120 days and evaluated the survival and relative growth rate (RGR). The substrate comprising of chopped polyurethane foam (T5) showed greater efficiency for growth in vitro and also increased survival rate, while substrate cassava starch (T3) provided delay for plantlet growth. Therefore, chopped polyurethane foam is recommended because of low cost and suitable characteristics for the propagation of Cattleya loddigesii.

From doubly labelled water to half-life; validating radio-isotopic rubidium turnover to measure metabolism in small vertebrates

The doubly labelled water method (DLW) is widely used to measure field metabolic rate (FMR), but it has some limitations. Here, we validate an innovative technique for measuring FMR by comparing the turnover of isotopic rubidium (86Rb kb) with DLW depletion and the rate of CO2 production (V·co2) measured by flow-through respirometry (FTR) for two dunnart species (Marsupialia: Dasyuridae), Sminthopsis macroura (17 g) and Sminthopsis ooldea (10 g). The rate of metabolism as assessed by V·co2 (FTR) and 86Rb kb was significantly correlated for both species (S. macroura, r2 = 0·81, P = 1·19 × 10-5; S. ooldea, r2 = 0·63, P = 3·84 × 10-4), as was V·co2 from FTR and DLW for S. macroura (r2 = 0·43, P = 0·039), but not for S. ooldea (r2 = 0·29, P = 0·168). There was no relationship between V·co2 from DLW and 86Rb kb for either species (S. macroura r2 = 0·22, P = 0·169; S. ooldea r2 = 0·21, P = 0·253). We conclude that 86Rb kb provided useful estimates of metabolic rate for dunnarts. Meta-analysis provided different linear relationships between V·co2 and 86Rb kb for endotherms and ectotherms, suggesting different proportionalities between metabolic rate and 86Rb kb for different taxa. Understanding the mechanistic basis for this correlation might provide useful insights into the cause of these taxonomic differences in the proportionality. At present, it is essential that the relationship between metabolic rate and 86Rb kb be validated for each taxon of interest. The advantages of the 86Rb technique over DLW include lower equipment requirements and technical expertise, and the longer time span over which measurements can be made. The 86Rb method might be particularly useful for estimating FMR of groups for which the assumptions of the DLW technique are compromised (e.g. amphibians, diving species and fossorial species), and groups that are practically challenging for DLW studies (e.g. insects). © 2013 British Ecological Society.

Sensor eletro-óptico de tensões elevadas e sua viabilidade para implementação de TP óptico

Pós-graduação em Engenharia Elétrica - FEIS

Modelagem matemática da doença do caranguejo letárgico via ondas viajantes

Pós-graduação em Biometria - IBB

Condições de propagação e revitalização para a produção de etanol pela linhagem IQAr/45-2 da levedura Saccharomyces cerevisiae em fermentação sucessivas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do uso de radiação micro-ondas e ultrassom combinados com agentes químicos como pré-tratamentos de bagaço de cana-de-açúcar para posterior sacarificação enzimática

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação de funções para modelagem do efeito da refratação ionosférica na propagação dos sinais GPS

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)