Effects of intensification of the amazon river prawn, macrobrachium amazonicum, grow-out on effluent quality

Studies to determine suitable levels of intensification are essential for developing sustainable aquaculture. The objective of this study was to evaluate the quality of effluents discharged from ponds stocked with 10 (D10), 20 (D20), 40 (D40), and 80 (D80) postlarvae of Macrobrachium amazonicum/m2. Intake and effluent water samples were taken throughout a 5.5-mo grow-out cycle. In that study, twelve 0.01-ha earthen ponds were stocked postlarvae with 0.01g. Average water exchange rate was 15%/d; water was discharged from the bottom of the ponds. Prawns were fed a commercial feed with 38% crude protein according to their biomass (3-10%) and the concentration of dissolved oxygen (DO). In our research, temperature, turbidity, total suspended solids, conductivity, DO, pH, biochemical oxygen demand (BOD), chemical oxygen demand (COD), N-ammonia, N-nitrite, N-nitrate, N-Kjeldahl nitrogen, total phosphorus, and soluble orthophosphate were measured every 15d throughout the experiment in the early morning (0630 to 0730h). Turbidity was lower in D10 than in D20 and D40 and total phosphorus was higher in D80 than in D10 and D20. An analysis of principal components comparing treatments and intake water showed three groups: intake, D10 and a cluster of D20, D40, and D80. On the basis of the water characteristics found in our study it appears that the farming of M. amazonicum is likely to have a low environmental impact, at least up to a stocking density of 80prawns/m2. © by the World Aquaculture Society 2013.

Effects of artificial substrate and night-time aeration on the water quality in Macrobrachium amazonicum (Heller 1862) pond culture

The effects of artificial substrate and night-time aeration on the culture of Macrobrachium amazonicum were evaluated in 12 ponds stocked with 45 prawns m-2. A completely randomized design in 2 × 2 factorial scheme with three replicates was used. The combination of factors resulted in four treatments: with substrate and aeration (SA), with substrate and without aeration (SWA), without substrate and with aeration (WSA) and without substrate and aeration (WSWA). The presence of substrate in SA and SWA treatments reduced suspended particles (seston) by ~17.3% and P-orthophosphate by ~50%. The use of aerator (WSA and SA treatments) significantly (P < 0.05) increased the concentration of dissolved oxygen, suspended particles and nutrients in the pond water. These results indicate that the effect of substrate on turbidity and total suspended solids (TSS) values is opposite to the effect of the aerator. The aerators in semi-intensive grow-out M. amazonicum farming lower water quality because they increased the amount of detritus and nutrients in the pond water. On the other hand, the use of artificial substrate reduces turbidity values, chlorophyll a, TSS and P-orthophosphate concentrations. Therefore, the combination of substrate addition and night-time aeration is not interesting because they have opposite effects. © 2013 John Wiley & Sons Ltd.

Subcellular localization and kinetic characterization of a gill (Na +, K+)-ATPase from the giant freshwater prawn macrobrachium rosenbergii

The stimulation by Mg2+, Na+, K+, NH 4 +, and ATP of (Na+, K+)-ATPase activity in a gill microsomal fraction from the freshwater prawn Macrobrachium rosenbergii was examined. Immunofluorescence labeling revealed that the (Na +, K+)-ATPase α-subunit is distributed predominantly within the intralamellar septum, while Western blotting revealed a single α-subunit isoform of about 108 kDa M r. Under saturating Mg2+, Na+, and K+ concentrations, the enzyme hydrolyzed ATP, obeying cooperative kinetics with V M = 115.0 ± 2.3 U mg-1, K 0.5 = 0.10 ± 0.01 mmol L-1. Stimulation by Na+ (V M = 110.0 ± 3.3 U mg-1, K 0.5 = 1.30 ± 0.03 mmol L -1), Mg2+ (V M = 115.0 ± 4.6 U mg -1, K 0.5 = 0.96 ± 0.03 mmol L-1), NH4 + (V M = 141.0 ± 5.6 U mg -1, K 0.5 = 1.90 ± 0.04 mmol L-1), and K+ (V M = 120.0 ± 2.4 U mg-1, K M = 2.74 ± 0.08 mmol L-1) followed single saturation curves and, except for K+, exhibited site-site interaction kinetics. Ouabain inhibited ATPase activity by around 73 % with K I = 12.4 ± 1.3 mol L-1. Complementary inhibition studies suggest the presence of F0F1-, Na+-, or K +-ATPases, but not V(H+)- or Ca2+-ATPases, in the gill microsomal preparation. K+ and NH4 + synergistically stimulated enzyme activity (≈25 %), suggesting that these ions bind to different sites on the molecule. We propose a mechanism for the stimulation by both NH4 +, and K+ of the gill enzyme. © 2013 Springer Science+Business Media New York.

Isolation and characterization of SNPs within HSC70 gene in the freshwater prawn Macrobrachium amazonicum

We report the discovery of 13 synonymous single nucleotide polymorphisms (SNPs) within a fragment of HSC70 gene in Macrobrachium amazonicum. Polymorphisms were assessed using the reference sequence of the HSC70 gene in Macrobrachium rosenbergii to the primers design. The minor allele frequency ranged from 0.011 to 0.213. None of the SNPs deviated significantly from Hardy-Weinberg equilibrium. These SNPs will be useful to access the genetic variation of populations and to the study of their relations with characteristics of interest for aquaculture. Both cases, favoring the conservation of the natural stocks of Amazon river prawn. © 2013 Springer Science+Business Media Dordrecht.

Desenvolvimento embrionário do camarão-da-amazônia Macrobrachium amazonicum (Heller, 1862) (Crustacea, Decapoda, Palaemonidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Balanço de nitrogênio em viveiros de crescimento final de Macrobrachium amazonicum com uso de aeração noturna e substratos artificiais

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Análise da produtividade e viabilidade econômica de quatro sistemas de berçários do camarão-da-Amazônia, Macrobrachium amazonicum

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da alimentação e densidade de estocagem no desempenho zootécnico e perfil celular do hepatopâncreas do camarão-da-amazônia Macrobrachium amazonicum (Heller, 1862) (Crustacea, Palaemonidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Avaliação microbiológica do camarão da Amazônia (Macrobrachium amazonicum) e sua relação com o ambiente de criação na carcinicultura

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Viabilidade técnica e econômica da larvicultura do camarão-da-amazônia, Macrobrachium amazonicum, em diferentes temperaturas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estratégias de povoamento e despesca no cultivo do camarão-da-amazônia Macrobrachium amazonicum: efeitos na estrutura populacional e na produção

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Intensificação do cultivo de Macrobrachium amazonicum: efeito das estratégias de estocagem e despesca na água dos viveiros, efluentes e sedimentação em viveiros de crescimento final

Pós-graduação em Aquicultura - FCAV

Efeito da intensificação na larvicultura do camarão-da-malásia Macrobrachium rosenbergii

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Controle social do crescimento do camarão-da-amazônia Macrobrachium amazonicum

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estrutura funcional do hepatopâncreas no processo digestivo em Macrobrachium amazonicum

Pós-graduação em Aquicultura - FCAV