Estado nutricional de pré-escolares e consumo alimentar de suas famílias no Município de Ponta Grossa-Pr: a influência das condições socioeconômicas e das políticas públicas

Pós-graduação em Alimentos e Nutrição - FCFAR

Hematological and biochemical changes in a Phrynops geoffroanus (Schweigger, 1812) population in response to environmental stressors

Turtles are among the most endangered vertebrate groups, and the main threats to populations are environmental pollution and habitat degradation. The species Phrynops geoffroanus, popularly known as “Geoffroy’s side-necked turtle”, has proliferated in polluted environments, where adverse conditions could influence their living habits and physiological condition. Studies that monitor the effects of environmental pollution are key to understanding the species’ biology and designing effective conservation strategies. Thus, the analysis of hematological and biochemical parameters has been shown to be important in assessing the health of wild animals and risks for the animal and ecosystem. This study aimed to assess the environmental influence on the physiology of a P. geoffroanus population through the evaluation of antioxidant status and responses to environmental stressors, compared to specimens from a place under controlled conditions. Blood samples of 60 specimens were collected, 30 from the Felicidade Stream, polluted environment, within the city of São José do Rio Preto, and 30 from the “Reginaldo Uvo Leone” breeding farm, Tabapuã, SP, a place under controlled conditions, whose samples constituted the control group. They were evaluated by hemogram and by determining thiobarbituric acid reactive species (TBARS), Trolox-equivalent antioxidant capacity (TEAC) and the activities of the antioxidant enzymes catalase and glucose-6-phosphate dehydrogenase (G6PDH). There was a wide variation in hematological parameters of P. geoffroanus from the urban environment. The red blood cell count and hemoglobin values were significantly less than those observed in animals from the breeding farm (P = 0.0004; P = 0.0371, respectively). There was a significant increase in the number of thrombocytes (P < 0.0001) and leukocytes (P < 0.0001) in the animals from Felicidade Stream. The stress indices were similar between the two groups (P = 0.4077). TBARS levels showed the cytotoxic potential of compounds in the urban environment, whose animals had elevated levels of lipid peroxidation (P < 0.0001), despite showing a response to environmental damages with increase in antioxidant capacity, as demonstrated by the TEAC assay (P = 0.0207). The lower catalase enzyme activity noted in individuals from the urban environment (P = 0.000184) could be due to the presence of inhibitory compounds. On the other hand, G6PDH activity was higher (P = 0.002962), where this enzyme acts in the generation of NADPH, which is used in several detoxification pathways. We conclude that environmental contamination can increase oxidative damages and generate physiological changes in this species. These data are very useful for the conservation of P. geoffroanus and turtles in general, and confirm that these techniques are effective in monitoring natural regions and that P. geoffroanus can serve as an environmental contamination bioindicator.

Influence of training status and eNOS haplotypes on plasma nitrite concentrations in normotensive older adults: a hypothesis-generating study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Qualidade de vida e satisfação das gestantes portadoras de diabete em uso de insulina em hospital universitário do interior de São Paulo

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Using SPE-LC-ESI-MS/MS analysis to assess disperse dyes in environmental water samples

We have optimized an SPE-LC-ESI-MS/MS method and used it to monitor disperse azo dyes in environmental aquatic samples. Calibration curves constructed for nine disperse dyes-Red 1, Violet 93, Blue 373, Orange 1, Orange 3, Orange 25, Yellow 3, Yellow 7 and Red 13-in aqueous solution presented good linearity between 2.0 and 100.0 ng mL(-1). The method provided limits of detection and quantification around 2.0 and 8.0 ng L(-1), respectively. For dyes at concentrations of 25.0 ng mL(-1), the intra- and interday analyses afforded relative standard deviation lower than 6 and 13%, respectively. The recovery values obtained for each target analyte in Milli-Q water, receiving waters and treated water samples spiked with the nine studied dyes at concentrations of 8.0, 25.0 and 50.0 ng L(-1) (n = 3) gave average recoveries greater than 70%, with RSD <20%. Statistical evaluation aided method validation. The validated method proved to be useful for analysis of organic extracts from effluents and receiving water samples after an SPE extraction step. More specifically, the method enabled detection of the dyes Disperse Red 1, Disperse Blue 373 and Disperse Violet 93 at concentrations ranging from 84 to 3452 ng L(-1) in the treated effluent (TE), affluent and points collected upstream and downstream of the drinking water treatment plant of a textile dye industry in Brazil.

Biorremoção de corante direto de efluente industrial simulado utilizando levedura liofilizada

One of the most primal ways of human work already known is the tessellation and ginning for the production of fabric and clothing - what used to be, back in those days, statement of power and status. The arrival of the Industrial Revolution - in the middle of the XVIII century at Britain - increased the textile industry production, and what used to be manufactured and hard to obtain, starts then to be produced in mechanical ways and large-scale. Despite all the boost given to the economy of an expanding capitalist market, it should be pointed out the consequences of this major industrialization, especially the environmental ones, more and more concerning nowadays. The emissions of waste - that sometimes could be toxic - in effluents can possibly contaminate the aquatic ecosystems, causing a huge damage to its fauna and flora, affecting therefore all the biodiversity, reaching inclusively the humans. To avoid these problems, a few strategies have been taking place in the attempt to eliminate - or at least reduce - the amount of dye found in the effluents, and as the textile industry constantly leaves waste, efficient methods - that present good results in a short period of time - with a low cost are needed. The present study will test the bioremoval capacity of yeast (Saccharomyces cerevisiae) in contact with dyes in a fix concentration, diluted in water with three different pH values. The tests will be done duplicate, and after the concentration analyses - made by spectrophotometry - it will be analyzed which pH shows major efficiency in the dye removal and what is the influence of the biomass in this process

Diagnóstico do plano de gestão de resíduos sólidos do município de Osasco (SP)

The management of solid waste has been a great challenge to public managers both because of its high operational costs and increasing waste generation. Besides environmental and public health harm, it involves high costs to be mitigated. It is necessary to seek solutions that focus on, amongst other matters, economic efficiency for a healthy environment. This could be reached with sustainable development, which has proven itself as an alternative model of development to the actual one in place. In order to ensure that sustentability is the goal of an activity, it is necessary to establish goals and to evaluate a system in order to track opportunities and correct possible mistakes, that is, it is necessary to elaborate a Waste Management Plan. However, in order to establish goals, it is also necessary a good description of the management of solid waste, thus diagnosis is a core aspect in the building of an specific law, as it will allow for the knowledge of the actual status of solid waste collection in Osasco town, showing what progress has been made in recent years, identifying current problems and generating the foundations to build future perspectives in the area. The present work has seeked to elaborate a diagnosis on waste management in Osasco municipality, presenting places for consultation and analysing how it meets the current legislation in place, enabling it to serve as a model for the elaboration of other diagnosis in other Brazilian municipalities

Construção de índice percentual de “status” de desenvolvimento sus-tentável de assentamentos rurais utilizando procedimento estatísti-co multivariado

The objective of the present work is the construction of percentual indexes of sustainable development "Status" - PIDSD (%) aiming to order and classify the rural settlements, considering agricultural, socioeconomic and environmental aspects, in order to diagnose their sustainable reality. This way, we considered multivariate statistical procedure to establish analytical descriptors - indexes - like the principal components technique (CP). The CP technique was used in a matrix formed by 47 variables observed in 50 rural settlements, distributed in seven different regions of the state of Mato Grosso, obtained from diagnostics, provided by "Mato-Grossense" Enterprise of Research, Assistance and Rural Extension S/A - EMPAER - MT, in order to obtain the indexes used in the construction of PIDSD (%). The settlements with higher PIDSD (%) were considered "higher potential" or "higher sustainable" in relation to the analyzed variables, making the establishment of assistance strategies and cooperation possible, allowing the government and civil society in general, to improve those with worse results ("lower potential" or "lower sustainable"), and search for ways to strengthen and multiply the results of the "higher potential" settlements. Vale do Seringal settlement had the best conditions in relation to the variables, mainly those of higher weigh and was considered the one with "higher potential". São Sebastião had the worst conditions and was considered "lower potential".