Diversidade da herpetofauna de uma área de Mata Atlântica do estado de Alagoas: a reserva particular da usina Porto Rico, Campo Alegre

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Viabilidade de embriões de piracanjuba (Brycon orbignyanus) submetidos a diferentes protocolos de resfriamento

Pós-graduação em Aquicultura - FCAV

Controle biológico de Brachiaria decumbens Stapf em área de reserva legal em processo de recuperação, na região do Pontal do Paranapanema, São Paulo, Brasil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Análise citogenética clássica e molecular em Chelonoidis carbonaria e Phrynops geoffroanus (Testudines)

Pós-graduação em Genética - IBILCE

Caracterização da diversidade genética, via marcador microssatélite, e constituintes do óleo essencial de Lychnophora pinaste MART

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reintrodução de mutuns-do sudeste Cra blumenbachii (Cracidade) na mata atlântica da Reserva Ecológica de Guapiaçu (Cachoeiras de Macacu, RJ, Brasil)

Pós-graduação em Ciências Biológicas (Zoologia) - IBRC

Contribuição do estudo ultrassonográfico (Modo e Doppler) de órgãos abdominais em gatos do mato (Leopardus tigrinus) híbridos: valores de referência

Pós-graduação em Medicina Veterinária - FMVZ

Florística vascular não arbórea de um fragmento de floresta estacional semidecidual no munícipio de Analândia - SP

Pós-graduação em Ciências Biológicas (Biologia Vegetal) - IBRC

Reprodução em cativeiro de piracanjuba Brycon orbignyanus assistida por marcador molecular como estratégia de conservação

Pós-graduação em Ciências Biológicas (Zoologia) - IBB

Rarity as an indicator of endangerment in neotropical frogs

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Preservation of wild feline semen by freeze-drying: experimental model

According to the Convention on International Trade in Endangered Species, 36 wild feline species are threatened by extinction or severely endangered, and to save them is the target of several conservation programs. This study aimed to assess the viability of the freeze-drying technique for domestic cat sperm cells, with the ultimate goal of transferring this technology to the wild feline species. The domestic cat is an excellent experimental model for wild felids. It is in this scenario that the freeze-drying process (low-temperature vacuum dehydration) of sperm cells shows its value in preserving male cats' germplasm. Results from membrane and DNA integrity analysis are promising and validates the use of frozen-dried sperm samples in intracytoplasmic sperm injections (ICSIs). Further studies are still necessary to evaluate the ICSI embryo production using domestic cat frozen-dried sperm and the possibility of using such technology with wild felines.

Oocyte maturation in bitches

The canine species has been used as an experimental model for preservation of endangered species. Biotechnologies of reproduction, such as in vitro maturation (IVM), have been used to meet this objective. Several protocols for in vitro embryo production (IVEP) in swine and bovine species have been adapted for canids. However, the highest rate reported for in vitro maturation in canids is only 39%, which is still lower than those in other species. Therefore, current research on assisted reproduction in canids have focused on several IVM protocols, including the addition of proteins, hormones, meiosis inhibitors, growth factors and antioxidants to the maturation media and the determination of suitable timing for culture, so that variables involved in the process can be fine-tuned. This review has the main objective of describing major developments and limitations in the process of oocyte maturation in bitches.

O estado da arte das vocalizações de anfíbios anuros da Mata Atlântica

The vocals are the most conspicuous means of communication between frogs and can express evolutionary relationships among species. Call descriptions of frogs, in general, not standard acoustic parameters which are greatly diversified and usually restricted to comparisons between closely related taxa. The Atlantic Forest is considered one of the biodiversity hotspots of the world, however, it appears that the number of species to be known is still very large. To anurans from this Brazilian Domain, vocalizations are complex and can vary intra and interpopulations. Thus, we presented a quantitative and qualitative analysis of the work descriptions of anuran advertisement call from the Atlantic Forest, available in the literature so far. The frog species from this Domain and their descriptions of the advertisement call have been raised in the literature, organized into families and threat category IUCN Red List Threatened Species. The analysis of the descriptions were made based on the presence of preset parameters. It was observed that the species which the advertisement call is described within the standard are expected within the Brazilian Atlantic Domain, but efforts to describe the advertisement call of frogs of the Atlantic are concentrated in non-threatened species. Among the descriptions works, there is a large discrepancy between the information presented, giving preference to the use of abiotic data in this kind of work, to the detriment of biotic data about choir where the songs were recorded. The results point to the need for advancements in the descriptions of the advertisement of endangered species or to those with deficient information on their conservation status in the Atlantic Forest, and also for greater communication between works that describe vocalizations of frogs. It will be important to know about the state of the art of descriptions works of frogs in other Brazilian Domains, and relate this information with knowledge...

State of the art on animal embryonic chimeras

Embryonic chimerism is generally used in basic research and in vivo diagnosis of undifferentiated embryonic stem cells (ESC), mostly using mice embryos, although there have been reports in the literature on using rat, rabbit, sheep, chicken, primate, bovine, goat and pig embryos. Several techniques can currently be used to produce chimeric embryos, including microinjection, co-culture with ESC, fusion and aggregation. Although microinjection is the most commonly used method in mice, the mere aggregation of embryos with ESC may result in viable chimeras and be as efficient as microinjection. In mice, this chimerism technique has been shown to have the advantage of aggregating embryos in different stages of development with different ploidy, in addition to using ESC in the tetraploid complementation assay. Compared to other techniques for producing chimeras, the aggregation technique is a cheaper, faster and easier methodology to be performed. Moreover, aggregation can be simplified by chemically removing the zona pellucida with pronase or acidic Tyrode’s solution and be enhanced by using the Well of the Well culture system in combination with adhesion molecules, such as phytohemagglutinin. The most commonly used stages for chimerism by aggregation are those that precede the full compaction of the morula. In these stages, embryos have low-tension adherent junctions at the tangential point between two blastomeres. During the embryonic development of mice, the inner cell mass differentiates into epiblast and hypoblast. These layers will originate the fetal tissues and a portion of the extraembryonic tissues (yolk sac, allantois and amnion), whereas the trophectoderm (TE) gives rise to the chorion. A functional TE is essential for the complex molecular communications that occur between the embryo and the uterus. Embryos produced by somatic cell nuclear transfer, such as commercial cattle clones or endangered species, are subject to large fetal and neonatal losses. Hence embryo complementation with heterologous TE could be of assistance to decrease these losses and might as well assist development of high-value embryos in other approaches.

Seasonal effects and antifungal activity from bark chemical constituents of Sterculia apetala (Malvaceae) at Pantanal of Miranda, Mato Grosso do Sul, Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)