DISTRIBUTION OF DIFFERENT FIBER TYPES OF M-EXTENSOR CARPI RADIALIS LONGUS OF THE RAT

As revealed by the NADH-diaphorase and myosine ATPase, the M. extensor carpi radialis longus of the rat possesses at least 3 main kinds of fibres, with different distribution on the superficial and deep portions of the muscle. The superficial portion revealed that 67.68 % are FG (fast-twitch-glycolytic) fibres, 14.72 % are FOG (fast-twitch-oxidative) fibres and 17.60 % are SO (slow-twitch-glycolytic) fibres. Already the deep portion revealed that 71.29 % are SO (slow-twitch-glycolytic) fibres, 17.46 % are FOG (fast-twitch-oxidative-glycolytic) fibres and 11.25 % are FG (fast-twitch-glycolytic) fibres. The miosine ATPase reaction was used to demonstrate contracting characteristics. These findings suggest that the movements of fast contraction of the M. extensor carpi radialis longus are predominant.

ANATOMICAL STUDY OF THE OPOSSUM (DIDELPHIS-ALBIVENTRIS) EXTRAOCULAR-MUSCLES

The anatomy of the extraocular muscles was studied in 10 adult opossums (Didelphis albiventris) of both sexes. Eight extraocular muscles were identified: 4 rectus muscles, 2 oblique muscles, the levator palpebrae superioris and the retractor ocular bulbi. The rectus muscles originate very close one to another between the orbital surfaces of the presphenoid and palatine bones. These muscles diverge on the way to their insertion which occurs at about 2 mm from the limbus. The levator palpebrae superioris originates with the dorsal rectus and is positioned dorsally in relation to it. The retractor ocular bulbi forms a cone which embraces the optic nerve and is located internally in relation to the rectus muscles. The dorsal oblique originates on the presphenoid bone and after a tendinous trajectory through a trochlea on the medial wall of the orbit, inserts into the ocular bulb. The only muscle arising from the anterior orbital floor is the ventral oblique. The main nerve supply for these muscles is the oculomotor, except for the dorsal oblique which is innervated by the trochlear nerve, and the lateral rectus which is innervated by the abducens nerve. The retractor ocular bulbi receives branches from the inferior division of the oculomotor nerve and some branches from the abducens nerve.

Electromyographic study in Erector spinae, Rectus abdominis, Glutaeous maximus and Rectus femuris muscles, in standing and static posture with load on hands

Observe the loads associates application with in position of body, in the static or dynamic postures. Methods: the electromyographic study in erector spinae, rectus abdominis, glutaeous maximus and rectus femoris muscles was accomplished in female volunteers from 18 have 27 years old, previously selected. The muscles electric activities was gotten with surface electrodes, in standing and static posture, with the parallels and horizontal upper limbs with load on their hands. Conclusion: In this study it was clearly observed influence of the load and distance there is over studied musculature associated with standing erect posture.

Influence of temperature upon paralyzing and myotoxic effects of bothropstoxin-I on mouse neuromuscular preparations

Bothropstoxin-I (BthTX-I), from B. jararacussu venom, is a phospholipase A(2) (PLA(2)) homologue devoid of enzymatic activity. Besides inducing severe myonecrosis, BthTX-I promotes paralysis of both directly and indirectly evoked contractions in isolated neuromuscular preparations. We applied an experimental paradigm in order to characterize the steps involved in the toxic effects of BthTX-I on mouse neuromuscular junction. Myotoxicity was assessed by microscopic analysis of extensor digitorum longus muscles; paralyzing activity was evaluated through the recording of isolated contractions indirectly evoked in phrenic-diaphragm preparations. After 90 min at 35 degreesC, BthTX-I induced complete and irreversible paralysis, and damaged 30.3 +/- 2.7% of muscle fibers. In contrast, no effect was observed when tissues were incubated with BthTX-I at 10degreesC for 60 min and subsequently washed with toxin-free solution and maintained at 35 degreesC. These results indicate that the binding of BthTX-I to the cellular tissue surface is very weak at low temperature and that an additional factor is necessary. However, when tissues were submitted to BthTX-I (10degreesC for 60 min), and the temperature was elevated to 35 degreesC, omitting the washing step, it was observed muscle paralysis and damage in 39.04 +/- 4.2% of muscle fibers. These results indicate that a temperature-dependent step is necessary for BthTX-I to promote both its myotoxic and paralyzing activities. (C) 2004 Elsevier B.V.. All rights reserved.

Effects of nandrolone decanoate and resistance exercise on skeletal muscle in adult male rats

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Histochemical and SEM evaluation of the neuromuscular junctions from alcoholic rats

In the present study morphological changes occurring in the neuromuscular junctions (NMJ) of the extensor digitorum longus (EDL) and soleus muscles from albino rats (Rattus norvegicus) submitted to experimental chronic alcoholism were evaluated. Seventy two male animals aged 4 months and weighing on average 400 g were divided into three groups: control, alcoholic and isocaloric. Six rats from each group were anesthetized and sacrificed after 5, 10, 15 and 18 months. The NMJ did not show detectable morphological changes in either muscle after treatment when examined by light microscopy. With respect to the dimensions, statistical analysis demonstrated a tendency to a statistically significant treatment x time interaction for the length of soleus muscle NMJ. The ultrastructural study, however, revealed that the NMJ of the soleus muscle of animals submitted to 18 months of experimental alcoholism presented important morphological alterations. Characteristically, the NMJ of these muscles is located on an elevation on the surface of the muscle fiber, presenting a regular round, oval or elliptical shape and continuous and not very deep synaptic grooves. Approximately 30% of the NMJ of alcoholic rats are irregular in shape, with the sarcolemmal elevations typical of the synapse region being flattened on at least one side, with discontinuous synaptic grooves, and deep and punctiform contacts of the synaptic buds. These data suggest that, although skeletal muscle has a greater natural resistance against the direct or indirect effects of alcohol, some submicroscopic morphological alterations are detectable in the NMJ, especially in muscles with oxidative metabolism (soleus) following long periods of ingestion of alcohol. (C) 2002 Elsevier B.V. Ltd. All rights reserved.

Anatomical aspects of the masticatory muscles of the tufted capuchin (Cebus apella)

The description of the macroscopic structure of the masticatory muscles is based upon the dissection of 26 adult and juvenile tufted capuchin monkeys (Cebus apella) of both sexes. A detailed description of the temporal, masseter and medial and lateral pterygoid muscles on each side of the head is given. Not only the general shape, origin and insertion are described, but also the architectonic organization, i.e. the stratiform disposition of the muscle parts. Anatomical variations in each sex or age appear to be few and unimportant. Anatomical aspects are found to be essentially similar to those found in other primates including man; however some characteristics differences do exist and deserve special comment.

Electromyographic study of levator scapulae and rhomboideus major muscles in movements of the shoulder and arm

In 21 normal adult male subjects, the muscular activity of the levator scapulae and rhomboideus major muscles was studied electromyographically during the movements of the shoulder and arm. Two single coaxial needle electrodes were used for registering the action potentials. Concerning shoulder movement, it was shown that the levator scapulae was active in elevation and rhomboideus major was active in retraction. Both muscles were inactive during protrusion, in most events. Concerning free movements of the arm, both muscles were active in abduction, elevation, adduction, flexion and circumduction, but inactive in extension. During the same movements, performed with a load, we observed greater intensity in the activity of these muscles in comparison to their activity during free movements.

Electromyographic study of the trapezius (middle portion) and rhomboideus major muscles in movements of the trunk

The authors studied the trapezius (middle portion) and rhomboideus major muscles in movements of flexion, extension, inclination and rotation of the trunk. The electromyographic records demonstrate that such muscles show activity only at the ending of flexion, being inactive in the other movements.

Analyse electromyographique des muscles levator scapulae et rhomboideus major dans les mouvements du membre superieur

The participation of the levator scapulae and rhomboideus major muscles in some movements of the upper limb was analysed in 21 young adult male volunteers. A 2 channel TECA TE4 electromyograph connected with single coaxial needle electrodes was used. In abduction, elevation, adduction, flexion and circumduction participation of both muscles in free movements of the upper limb was found. In extension, however, these muscles were inactive. In the same movements analysed with load, a major intensity of action of these muscles was registered as compared with those obtained in free movements.

Electromyographic study of the trapezius (pars media) and rhomboideus major muscles during respiration

The authors studied the trapezius (pars media) and rhomboideus major muscles during deep inspiration and expiration. The electromyographic records demonstrated that these muscles showed no activity in either phase of breathing.

Electromyographic study of the trapezius (pars media) and rhomboideus major muscles in free movements of the head

The authors studied the trapezius (pars media) and rhomboideus major muscles in movements of flexion, extension, inclination and rotation of the head. The electromyographic records demonstrated that referred muscles are inactive in these different movements.

Etude electromyographique des muscles trapezius (partie moyenne) et rhomboideus major sousmis a tension isometrique

The participation of the trapezius (middle portion) and rhomboideus major muscles submitted to an isometric tension (holding downward dumbells of 11, 15 and 19 kg) was analysed in 40 young adult male vounteers. A 2 channel TECA TE4 electromyograph connected with single coaxial needle electrodes was used. In the initial phase of the test, holding downward, generally the trapezius and rhomboideus major muscles showed activity and no activity, respectively. In the cases where activity was present during the downward positions it was reduced gradually until complete rest.