Avaliação do efeito osteoindutivo do PDGF-BB associado a diferentes carreadores na regeneração óssea em cavidades cirurgicamente criadas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito do ácido tranexâmico associado à cola de fibrina sobre o reparo ósseo: estudo histológico em ratos

The bone repair process is controlled by complex molecular mechanisms that involve systemic and local factors. Fibrin glue is derived from human plasma and mimics the final pathway of coagulation network. Tranexamic acid inhibits fibrinolysis and prevents or decreases the formation of degradation products of fibrin and fibrinogen. The purpose of this study was to evaluate histologically in rats the effect of tranexamic acid associated with the fibrin glue on bone healing. The experiment used 60 (n = 5) male rats in: GI: Control, GII: fibrin glue, GIII tranexamic acid and GIV /fibrin glue/tranexamic acid. Bone defect (2.5mm diameter) was created in right tibia. The animals were euthanized at 7,14 and 30 days postoperatively, and the pieces were processed with hematoxylin and eosin. The results showed at 7 days post-operative surgical cavity filled with dense connective tissue rich in fibroblasts, permeated by delicate neoformed bone trabeculae in percentage of 70-80% for GI, GII and GIII and GIV to 94.8%. At 14 days post-operative newly formed bone was found between 75-85% for GI, GII and GIII and percentage above 95% for GIV. At 30 days postoperative GI and GIV showed 95-100% of mature bone tissue; GII and GIII in percentage close to 80-90%. Based on the results and methodology is concluded that fibrin / tranexamic acid glue association has positive action on bone repair.

Osseointegração de implantes instalados sem estabilidade primária: o papel dos materiais à base de fibrina e fosfato de cálcio

When implants are installed immediately after tooth extraction may occur anchoring primary decreased, delay or failure of osseointegration process. This occurs because of the large interface between the surrounding walls of the socket and the surface of the implant. For reconstruction, replacement or filling of bone defects the solution can be obtained with the use of autogenous, heterogenous or allogeneic bone grafts. However, these grafts suffer certain drawbacks, particularly a high rate of donor site morbidity, limited amounts of available bone, and the additional operative time required for harvest. For these reasons, intensive efforts have been directed toward developing alternative substances for to either augment or substitute. In this paper, we will examine some of the commonly used materials : fibrin and calcium phosphate.

Avaliação da incorporação de enxerto osseo homogeno fresco congelado em humanos: análise histologica e imuno-histoquímica

Allogeneic, fresh-frozen bone has been used in order to replace bone autografts. However, its osteoinduction and osteoconduction properties are not well-defined in the scientific literature. This work aimed to evaluate samples of homogenous bone grafts in humans by qualitative histological and immunohistochemical analysis. For this, ten pre-selected patients underwent surgical augmentation of bone defects. The homogenous fresh frozen block bone graft was stabilized and fixed by bicortical screws. After six months, the reopening procedure was performed for installation of osseointegrated implants. At this time surgical bone graft samples were removed by means of drill trephine. The samples were fixed in 10% formalin, processed with decalcified paraffin, and stained with hematoxylin and eosin. Immunohistochemistry was performed for the expression of Caspase 3 enzyme. The slides were brought to light microscopy for qualitative histology and immunohistochemistry. The results showed non-vital bone tissue, with few areas of deposition of new bone formation on the amorphous matrix, presence of chronic inflammatory infiltrate with areas of osteomyelitis, and expressive immunolabeling of Caspase 3. Given the methods employed and the results it was concluded that the allograft fresh-frozen block is not incorporated into the recipient bed after a healing period of six months.

Estudo comparativo do desempenho de diferentes membranas oclusivas de Látex Natural no reparo ósseo

Introduction: In dentistry, alveolar bone resorption is a limiting factor in the well being of individuals directly interfering in the stomatognathic system, causing problems in the context of overall health. Aiming to promote biological methods that can stimulate bone regeneration, several biomimetic strategies have been developed by the use of diverse materials possible to the bone matrix, culminating in the development of techniques that promote such repair. Objective: This work is a comparative study of the performance of films made with latex as occlusive membrane for Guided Bone Regeneration (GBR) procedure in three preparations: Latex preserved in ammonia, produced by Latex rubber clones IAN873 and PR255 polymerized immediately after collection and without use ammonia as a preservative. Methods: Sixty Wistar rats were randomly divided into 4 groups of 15 animals in which bone defects of critical size (8mm diameter) were made surgically in the skull. Group A was treated by GBR through the membrane latex preserved with ammonia, Group B received the membrane made of latex from IAN873, Group C, the membrane clone PR255 and group D was not treated by GBR. After a period of 7, 15 and 50 days, 5 animals from each group were euthanized, and specimens containing bone defect collected for microscopic examination (descriptive histology and histomorphometry). Results: The results showed that after 50 days there was bone formation in higher proportions in group D (p <0.05, ANOVA followed by Tukey), suggesting that further experiments should be conducted to conclude about the presence of ammonia and the influence of kind of rubber. Conclusion: GOR is a procedure proven effective in the treatment of bone defects. Therefore, further experiments should be conducted to reach a conclusion regarding the presence of ammonia in the latex composition for the manufacture of membranes, as well as the difference induced by the species of rubber.

Cimentos bioativos injetáveis funcionalizados com peptídeo osteogênico para reparação óssea

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação in vivo do efeitos do osso bovino desproteinizado revestido com estrôncio sobre o reparo ósseo

Pós-graduação em Ciências Odontológicas - FOAR

Análises físico-químicas e biológicas de diferentes scaffolds à base de polihidroxibutirato e polihidroxibutirato-co-valerato

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação do cimento de fosfato-carbonato de cálcio no reparo ósseo de defeitos não-críticos em tíbia de rato: análise histológica, histométrica e imunoistoquímica

Pós-graduação em Odontologia - FOA

Avaliação da influência do plasma rico em plaquetas e do laser de baixa intensidade na cicatrização de defeitos de fenestração periodontal em ratos: estudo histológico e histométrico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Processo de cicatrização óssea em defeitos cirúrgicos de tamanho crítico tratados com partículas de vidro bioativo associadas ou não à barreira de sulfato de cálcio: estudo histológico e histométrico em calvárias de rato

Pós-graduação em Odontologia - FOA

Avaliação da influência do ABM/P-15 “flow” na cicatrização de defeitos de fenestração periodontal em ratos: estudo histológico e histométrico

Pós-graduação em Odontologia - FOA

Avaliação da microbiota associada a osteomielite crônica dos maxilares através de meio de cultura e PCR

Pós-graduação em Odontologia - FOA

Processo de cicatrização óssea em defeitos cirúrgicos de tamanho crítico tratados com partículas do novo vidro bioativo associadas ou não à barreira de sulfato de cálcio: estudo histológico e histométrico em calvárias de rato

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Reconstrução imediata de diferentes defeitos peri-implantares em implantes pós-exodônticos: análise histomorfométrica em cães

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)