Expression of a new Bacillus thuringiensis Cry1Ia gene in Escherichia coli with strong activity against cotton pests

The control of cotton pests may be accomplished using Bacillus thuringiensis Cry proteins. For this purpose, the objective of this work was to evaluate the insecticidal activity of a new Cry1Ia protein against neonatal larvae of Spodoptera frugiperda and Anthonomus grandis. The complete cry1Ia gene, previously obtained by PCR with oligonucleotide primers based on the sequenced gene, was cloned into the vector pET28a(+), introduced into Escherichia coli BL21(DE3) and expressed by induction with IPTG. The expression of the Cry1Ia protein was confirmed with molecular weight of approximately 81 kDa. The results demonstrated the efficiency of the bacterial system for the expression of B. thuringiensis Cry1Ia protein, which was subsequently used in quantitative bioassays against S. frugiperda and A. grandis larvae, resulting in an extremely toxic protein for both species. This characteristic is exceptionally important for obtaining transgenic cotton plants resistant to these pests.

Identification of foliar diseases in cotton crop

The pathogens manifestation in plantations are the largest cause of damage in several cultivars, which may cause increase of prices and loss of crop quality. This paper presents a method for automatic classification of cotton diseases through feature extraction of leaf symptoms from digital images. Wavelet transform energy has been used for feature extraction while Support Vector Machine has been used for classification. Five situations have been diagnosed, namely: Healthy crop, Ramularia disease, Bacterial Blight, Ascochyta Blight, and unspecified disease. © 2012 Taylor & Francis Group.

Insect/Bt-cotton interactions: Are immunological variables and hemocyte ultrastructure in Podisus nigrispinus (Hemiptera: Pentatomidae) affected?

Recent studies have shown that ingestion by the army worm Spodoptera frugiperda of Cry1Ac toxin from Bt cotton promotes histochemical and ultrastructural changes in the digestive cells of the predatory pentatomid bug Podisus nigrispinus. Therefore, mindful of the changes in the midgut of the predator, which represents the first line of defence in this insect, our aim was to test the hypothesis that the consumption of Bt cotton-fed S. frugiperda by P. nigrispinus might lead to alterations in components of the immune system of P. nigrispinus. The Cry1Ac toxin level in the leaves of Bt cotton, nitric oxide, phenoloxidase activity, and total proteins were quantified by ELISA. Total and differential hemocyte counts were evaluated, and hemocyte ultrastructure analysis was undertaken. We found that ingestion of the prey fed daily with approximately 23 ± 0.70 ng g-1 Cry1Ac by wet weight of leaves, and expressed by the Bt cotton, induces small ultrastructural changes in the predator's granulocytes and plasmatocytes. However, these changes did not affect the total number and differential and humoral variables analyzed for the bug's hemocytes. © 2013 Copyright Taylor and Francis Group, LLC.

Genotoxicological assessment of two reactive dyes extracted from cotton fibres using artificial sweat

Human eyes have a remarkable ability to recognize hundreds of colour shades, which has stimulated the use of colorants, especially for clothing, but toxicological studies have shown that some textile dyes can be hazardous to human health. Under conditions of intense perspiration, dyes can migrate from coloured clothes and penetrate into human skin. Garments made from cotton fabrics are the most common clothing in tropical countries, due to their high temperatures. Aiming to identify safe textile dyes for dyeing cotton fabrics, the genotoxicity [in vitro Comet assay with normal human dermal fibroblasts (NHDF), Tail Intensity] and mutagenicity [Salmonella/microsome preincubation assay (30 min), tester strains TA98, TA100, YG1041 and YG1042] of Reactive Blue 2 (RB2, CAS No. 12236-82-7, C.I. 61211) and Reactive Green 19 (RG19, CAS No. 61931-49-5, C.I. 205075) were evaluated both in the formulated form and as extracted from cotton fibres using different artificial sweats. Both the dyes could migrate from cotton fibres to sweat solutions, the sweat composition and pH being important factors during this extraction. However, the dye sweat solutions showed no genotoxic/mutagenic effects, whereas a weak mutagenic potential was detected by the Ames test for both dyes in their formulated form. These findings emphasize the relevance of textile dyes assessment under conditions that more closely resemble human exposure, in order to recognize any hazard. © 2013 Elsevier Ltd. All rights reserved.

Thermophysical properties of cotton, canola, sunflower and soybean oils as a function of temperature

Vegetable oils are used in the industry of processed food, including deep-fat frying. This work determined data on the thermophysical properties of cotton, canola, sunflower, corn, and soybean oils. Thermal conductivity, heat capacity, density, and viscosity were measured within the temperature range of 299.15-433.15 K. The data showed that the temperature influenced the thermophysical properties of the oils studied. The developed correlations could be used to predict these properties within the range of temperatures studied. © 2013 Copyright Taylor and Francis Group, LLC.

O Testamento de Dona Balbina: um estudo de caso sobre escravidão e propriedade em Guarapuava (1851-1865)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Machado de Assis e o Espiritismo: diálogos machadianos com a doutrina de Allan Kardec (1865 . 1896)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Produção e qualidade da fibra do algodoeiro (Gossypium hirsutum L.) em função da adubação foliar com nitrato de potássio

Pós-graduação em Agronomia (Agricultura) - FCA

O final de uma guerra e suas questões logísticas: o conde D'Eu na guerra do Paraguai (1869-1870)

Pós-graduação em História - FCHS

Biologia de Paracoccus marginatus (Hemiptera: pseudococcidae) e de seu parasitóide Acerophagus papayae (Hymenoptera: encyrtidae ) em diferentes plantas hospedeiras e levantamento de inimigos naturais em Barbados

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ocorrência de espécies de Atta Fabricius, 1804 e Acromyrmex Mayr, 1865 em algumas regiões do Brasil

Pós-graduação em Agronomia (Proteção de Plantas) - FCA

The management of cover plant residues for cotton cropped in a no-tillage system

The cutting of plant residue in no-tillage systems under certain environmental conditions becomes necessary to adequately establish and grow crops. This study aims to assess the effect on the yield of different methods of managing millet plant residue in cotton plantations. The study was conducted during the agricultural years 2006/07 and 2007/08, and the treatments included no-mechanical-treatment tillage and the use of a rotary shredder, crimper-roller, and mechanical disintegrator for millet plants before sowing the cotton. Evaluations were performed for the residue fragmentation, emergence speed, percent of soil cover during the cycle and yield of the cotton crop. The emergence speed was faster in the management with the rotary shredder. In 2006/07, the no-tillage treatment showed a rate of loss for soil cover that was 46 percent greater than the disintegrator treatment. The rotary shredder and the disintegrator yielded greater soil coverage during the cultivation cycle, and the yield was highly correlated with the soil cover at 75 days after emergence. The management of the millet residue affected the cotton plants for the two-year study period.

Biological aspects of Aphis gossypii Glöver, 1877 (Hemiptera: Aphididae) on colored lint cotton cultivars

This paper outlines the results of a study into the biological aspects of Aphis gossypii Glöver on colored cotton cultivars through the construction of life tables. In addition, we evaluate the influence of density of gossypol glands and trichomes of leaves on the biology of the aphid. Tests were conducted in a climate chamber at 25 ± 2°C, with relative humidity (RH) at 70 ± 10%, and a photophase of 12 hours, using the cultivars BRS Rubi, BRS Safira, and BRS Verde. Newly hatched nymphs were individually isolated in petri dishes containing leaf discs of cotton cultivars on a layer of water-agar (1%) of approximately 5 mm thick. An evaluation of the trichomes and gossypol gland densities of the cotton leaf plants was performed under a stereomicroscope, delimited to an area of 1 cm², and a count and identification of those on the surface was made. The feed substrates that were evaluated influenced the nymphal stage of A. gossypii. BRS Verde provided the shortest duration and BRS Safira was the longest during this phase. The cultivar BRS Verde, with the lowest density of trichomes, provided a large number of nymphs and led to a higher net reproductive rate (R0). Given these results, conclusions can be drawn that the colored cotton cultivars influence the duration of nymphal and adult stages of A. gossypii. The cultivar with a high density of trichomes on leaves (BRS Safira) adversely affects the fertility parameters of A. gossypii.

Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in cotton: vertical distribution of egg masses, effects of adult density and plant age on oviposition behavior

O trabalho teve por objetivo determinar, em casa-de-vegetação, a densidade adequada de adultos, a idade ideal das plantas e a distribuição vertical de ovos nas diferentes partes da planta, visando à realização de estudos de resistência e à melhoria das práticas de manejo de Spodoptera frugiperda em algodoeiro. Os experimentos de oviposição de S. frugiperda em relação à densidade de adultos, relação entre plantas de algodoeiro e oviposição de S. frugiperda e não preferência para oviposição de S. frugiperda em variedades de algodoeiro foram realizados com plantas da variedade de algodoeiro BRS Ita 90. A não preferência (antixenose) para oviposição foi acompanhada nas variedades FiberMax 966, FiberMax 977, DeltaOpal, DeltaPenta, Acala 90, Coodetec 408, Coodetec 409, Coodetec 410, BRS Cedro, BRS Ipê, BRS Aroeira, IPR 96, IPR 20, BRS Araçá, IAC 24 e BRS Ita 90. Concluiu-se que S. frugiperda prefere ovipositar em plantas com cerca de 60 dias de idade, na superfície inferior das folhas situadas no terço superior dos testes de plantas sob condições de estufa. Uma densidade de, pelo menos, três pares de adultos S. frugiperda por planta foi suficiente para realizar testes de não preferência para oviposição em casa de vegetação. As variedades Coodetec 408, BRS Aroeira, BRS Araçá, BRS Ita 90 e DeltaPenta apresentaram resistência do tipo não preferência para oviposição de S. frugiperda. Em regiões com altas infestações de S. frugiperda, seria prudente para o cultivo utilizar a variedade de algodão BRS Ita 90.

Releasing number of Telenomus remus (Nixon) (Hymenoptera: Platygastridae) against Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) in corn, cotton and soybean

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)