Cannabidiol administration into the bed nucleus of the stria terminalis alters cardiovascular responses induced by acute restraint stress through 5-HT1A receptor

Systemic administration of cannabidiol (CBD) is able to attenuate cardiovascular responses to acute restraint stress through activation of 5-HT1A receptors. Previous results from our group suggest that the bed nucleus of the stria terminalis (BNST) is involved in the antiaversive effects of the CBD. Moreover, it has been proposed that synapses within the BNST influence restraint-evoked cardiovascular changes, in particular by an inhibitory influence on the tachycardiac response associated to restraint stress. Thus, the present work investigated the effects of CBD injected into the BNST on cardiovascular changes induced by acute restraint stress and if these effects would involve the local activation of 5-HT1A receptors. The exposition to restraint stress increased both blood pressure and heart rate (HR). The microinjection of CBD (30 and 60nmol) into the BNST enhanced the restraint-evoked HR increase, in a dose-dependent manner, without affecting the pressor response. The selective 5-HT1A receptor antagonist WAY100635 by itself did not change the cardiovascular responses to restraint stress, but blocked the effects of CBD. These results showed that CBD microinjected into the BNST enhanced the HR increase associated with acute restraint stress without affecting the blood pressure response. Although these results are not in agreement with those observed after systemic administration of CBD, they are similar to effects observed after reversible inactivation of the BNST. Moreover, similar to the effects observed after systemic administration, CBD effects in the BNST seem to depend on activation of 5-HT1A receptors. © 2012 Elsevier B.V. and ECNP.

Experimental study of bottom feed updraft gasifier

The updraft biomass gasifiers currently available produce a gas with high tar content. For almost all downstream applications a substantial reduction of the tar concentration is required. The gravimetric tar concentration behavior in producer gas, obtained at a modified updraft fixed bed gasifier, was studied. The feedstock feeding system was modified respect to the traditional updraft gasification design in order to decrease the tar concentration in the producer gas; the material is feeding continuously through a conduit in the base of the reactor over the grate. The caloric power of the syngas obtained was slightly lower than the typical value for this type of reactor and the highest efficiency obtained for the woodchip gasification was 77%. The highest tar concentration obtained during the experiments was 1652.7 mg N m-3 during the first our of experiments, comparable with the smaller value reported for the updraft reactors, this value is reduced significantly after the stabilization of the gasification process in the reactor. The smaller value obtained was 21 mg N m-3. © 2013 Elsevier Ltd.

Role of the bed nucleus of the stria terminalis in cardiovascular changes following chronic treatment with cocaine and testosterone: A role beyond drug seeking in addiction?

Neural plasticity has been observed in the bed nucleus of the stria terminalis (BNST) following exposure to both cocaine and androgenic-anabolic steroids. Here we investigated the involvement of the BNST on changes in cardiovascular function and baroreflex activity following either single or combined administration of cocaine and testosterone for 10 consecutive days in rats. Single administration of testosterone increased values of arterial pressure, evoked rest bradycardia and reduced baroreflex-mediated bradycardia. These effects of testosterone were not affected by BNST inactivation caused by local bilateral microinjections of the nonselective synaptic blocker CoCl2. The single administration of cocaine as well as the combined treatment with testosterone and cocaine increased both bradycardiac and tachycardiac responses of the baroreflex. Cocaine-evoked baroreflex changes were totally reversed after BNST inactivation. However, BNST inhibition in animals subjected to combined treatment with cocaine and testosterone reversed only the increase in reflex tachycardia, whereas facilitation of reflex bradycardia was not affected by local BNST treatment with CoCl2. In conclusion, the present study provides the first direct evidence that the BNST play a role in cardiovascular changes associated with drug abuse. Our findings suggest that alterations in cardiovascular function following subchronic exposure to cocaine are mediated by neural plasticity in the BNST. The single treatment with cocaine and the combined administration of testosterone and cocaine had similar effects on baroreflex activity, however the association with testosterone inhibited cocaine-induced changes in the BNST control of reflex bradycardia. Testosterone-induced cardiovascular changes seem to be independent of the BNST. © 2013 IBRO.

Continuous production of xylooligosaccharides in a packed bed reactor with immobilized-stabilized biocatalysts of xylanase from Aspergillus versicolor

The production of xylooligosaccharides (XOS) using a packed-bed enzymatic reactor was studied at lab-scale. For this, a xylanase from Aspergillus versicolor was immobilized on different supports. The optimal derivative was xylanase immobilized on glyoxyl-agarose supports. This derivative preserved 85% of its catalytic activity; it was around 700-fold more stable than the soluble enzyme after incubation at 60. °C and was able to be reused for at least 10 1. h-cycles retaining full catalytic activity. About 18% of oligosaccharides with prebiotic interest (X2-X6) were produced by the glyoxyl derivative in batch hydrolysis. The production of xylobiose was 2.5-fold higher using the immobilized preparation than with soluble enzyme and small concentrations of xylose (<0.1%) were observed only at the end of the reaction. The derivative was employed on a packed bed reactor, and the continuous operation with no recirculation reached 56% and 70% of the end of reaction with flow rates of 60. mL/h and 12. mL/h, respectively. In continuous operation with recirculation at a flow rate of 60. mL/h, the reaction was completed after four hours. © 2013 Elsevier B.V.

Comparação da estabilização da manipueira com calcário e hidróxido de sódio na fase acidogênica da biodigestão anaeróbia e uso do biofertilizante

Pós-graduação em Agronomia (Energia na Agricultura) - FCA

Obtenção de parâmetros físicos e térmicos para simulação e projeto de bioreatores de fermentação em estado sólido em leito fixo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desempenho de reatores anaeróbios horizontais com manta de lodo e de leito fixo, em série, tratando águas residuárias do beneficiamento do café por via úmida

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização e estudo do comportamento térmico de lodo proveniente da indústria sucroalcooleira

Pós-graduação em Química - IBILCE

Influência da relação entre diâmetros no comportamento hidrodinâmico de reatores aeróbios de leito fluidizado com circulação em tubos concêntricos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da concentração do meio suporte e relação entre áreas no desempenho de reatores aeróbios de leito fluidizado com circulação interna no tratamento de esgoto doméstico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Remoção de nitrogênio de água residuária de produção intensiva de tilápias com recirculação utilizando reator de leito fluidizado com circulação em tubos concêntricos

Pós-graduação em Engenharia Civil - FEIS

Avaliação de sistemas de tratamento e reúso de esgoto sanitário para fins de irrigação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Performance of UASB reactors in two stages followed by post-treatment with activated sludge in wastewater batch of wet-processed coffee

In this study it was evaluated the efficiency of the treatment of wet-processed coffee wastewater in upflow anaerobic sludge blanket (UASB) reactors in two stages, in bench scale, followed by post-treatment with activated sludge in batch. The first UASB reactor was submitted to an hydraulic retention time (HRT) of 6.2 d and organic loading rates (OLR) of 2.3 and 4.5g CODtotal (L d)-1, and the second UASB reactor to HRT of 3.1 d with OLR of 0.4 and 1.4g CODtotal (L d)-1. The average values of the affluent CODtotal increased from 13,891 to 27,926mg L-1 and the average efficiencies of removal of the CODtotal decreased from 95 to 91%, respectively, in the UASB reactors in two stages. The volumetric methane production increased from 0.274 to 0.323L CH4 (L reactor d)-1 with increment in the OLR. The average concentrations of total phenols in the affluent were of 48 and 163mg L-1, and the removal efficiencies in the UASB reactors in two stages of 92 and 90%, respectively, and increased to 97% with post-treatment. The average values of the removal efficiencies of total nitrogen and phosphorus were of 57 to 80% and 44 to 60%, respectively, in the UASB reactors in two stages and increased to 91 and 84% with the post-treatment.

Anaerobic-aerobic treatment of swine wastewater in uasb and batch reactors in series

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Fluid-dynamic assessment of sugarcane bagasse to use as feedstock in bubbling fluidized bed gasifiers

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)