232 resultados para Anadenanthera e Viabilidade
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The experiment had the aim of quantifying seasonal chlorophyll variation on leaves of Anadenanthera colubrina and Tabebuia avellanedae. The experiment used a randomized block design established as a 2 x 3 factorial (two species and three sampling seasons) with 5 replications. The concentrations of chlorophyll in leaves were performed at the end of winter, late spring, and summer. The content of chlorophyll a changed only for A. colubrina, bringing up from spring (117.52 mu g mL(-1)) to summer (151.13 mu g mL(-1)). For the two species, the proportion of chlorophyll b was higher between periods at the end of spring (average of 29.31 mu g mL(-1)) to the end of summer (average of 74.96 mu g mL(-1)) with close relationship with the decrease in global solar irradiance. The chlorophyll a/b relationship increased in late spring due to reduction of chlorophyll b content. A. colubrina showed greater adaptation to the studied environment.
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The putative translation initiation factor 5A (eIF5A) is a highly abundant and conserved protein in all eukaryotes and archaebacteria. This factor is essential for cell viability and is the only cellular protein known to contain the unusual amino acid residue hypusine. In Saccharomyces cerevisiae eIF5A is expressed in aerobic conditions by the gene TIF51A. Although eIF5A has been known for almost 30 years, the biological role of this protein is still obscure. This article reviews the research on the function of eIF5A, discussing the evidence for its involvement in various steps of mRNA metabolism, including translation initiation, nucleocytoplasmic transport and mRNA decay. Moreover, it indicates other studies that have associated eIF5A with cell proliferation and cell cycle progression. Finally, this review presents recent results obtained in our laboratory that reemphasize the role of eIF5A in the translation scenario. Further experiments will be necessary to define the role played by eIF5A in the translational machinery.
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Type-1 diabetes patients suffer from frequent episodes of acidosis caused by an increased fatty acid metabolism and consequently increased plasma level of acetoacetate (AcAc) and β-hydroxybutyrate (β-HOB). This article describes a study of the effects of pathological concentrations of AcAc and β-HOB on lipoperoxidation, cell viability and the release of the CXCL8 (IL-8) cytokine by activated neutrophils. Neutrophils from healthy donors were isolated by density gradient (Histopaque® 1077/1119) and incubated with the ketone bodies. Lipoperoxidation was determined as thiobarbituric acid reactive substances (TBARS). The cell viability was evaluated by the release of intracellular lactate dehydrogenase. The release of CXCL8 was measured by ELISA in a 24-h culture of opsonized zymosan-stimulated neutrophils. AcAc, but not β-HOB, provoked a dose-dependent increase in the neutrophil membrane lipoperoxidation (p<0.05; r =0.9915). In the cytotoxicity assay, a dose-dependent release of LDH was observed when the neutrophils were incubated with AcAc in concentrations up to 40 mM (p<0.05). β-HOB was devoid of effect. The release of CXCL8 was inhibited by AcAc and β-HOB in a dose-dependent manner. In conclusion, these results suggest that the accumulation of ketone bodies in diabetic patients could be involved in their usually increased susceptibility to infection.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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The region of Alfenas, in the state of Minas Gerais, Brazil, is predominantly constituted of Pre-Cambrian rocks with well developed alteration profiles in association with colluvial and alluvial sediments. No study to date has examined in detail its potential use in the ceramic industry. The scarce knowledge of its mineralogical and technological properties limits its value and consequently its industrial use. Until now, these clay materials have been used in a rudimental manner, in small scale in the fabrication of red tiles. The present study aimed at analyzing these clays mineralogically (X-ray diffraction), chemically (major and minor elements by X-ray fluorescence and organic carbon analysis) and technologically (pressing granulometric distribution; mechanical resistence; water absorption, apparent porosity; linear firing shrinkage; color of firing and others) in order to better understand the raw material and develop adequate technological applications. The best results of ceramic properties were the samples with higher organic content (more plastic clays) and higher values of Al2O3 (kaolinite and gibbsite) and Fe2O3 as well lower SiO2 content and finer grain size which contribute to a better sinterization.
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The growth of large cities is usually accelerated and disorganized, which causes social, economical and infrastructural conflicts and frequently, occupation in illegal areas. For a better administration of these areas, the public manager needs information about their location. This information can be obtained through land utilization and land cover maps, where orbital images of remote sensing are used as one of the most traditional sources of data. In this context, the present work tested the applicability of the object-based classification to categorize two slum areas, taking into account the structure of the streets, size of the huts, distance between the houses, among other parameters. These area combinations of physical aspects were analyzed using the image IKONOS II and the software eCognition. Slum areas tend to be, to the contrary of the planned areas, disarranged, with narrow streets, small houses built with a variety of materials and without definition of blocks. The results of land cover classification for slum areas are encouraging because they are accurate and little ambiguous in the classification process. Thus, it would allow its utilization by urban managers.
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Piptadenia moniliformis Benth. is a tree species which is important for beekeeping, as well as being recommended for soil restoration, reforestation, wood production for small civil construction projects, and cattle and sheep forage. Information on how to evaluate seed physiological quality is still scarce and in this was the study aimed to adapt the procedures of the tetrazolium test to assess the viability of P. moniliformis seeds. Four seed lots were scarified in sulphuric acid for 30 min, and soaked between paper towels at 25 °C for 24 hours. The seed coat was then removed and the naked seeds immersed in tetrazolium solutions with concentrations of 0.05, 0.075 and 0.1% for 2, 3, and 4 hours at 35 oC in the dark. Each treatment consisted of four replications of 25 seeds. The embryos were classified according to viability based on the staining patterns. The previous soaking of the seeds for 24 hours at 25 oC between paper towels, followed by the removal of the seed coat and staining of the naked seeds for 4 hours in a 0.075% tetrazolium solution at 35 oC was the most efficient method for evaluating the viability of P. moniliformis Benth seeds.
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Anadenanthera macrocarpa (Benth.) Brenan is a tree species belonging to the family Leguminosae-Mimosoideae which is popularly known as mimosa-black. It features characteristics of early successional, natural occurrence in Brazil and can form clusters almost homogeneous, with great potential in the recovery of degraded areas. This study was conducted at the Center for Agricultural Sciences, University Federal of Alagoas state, aiming at the physical and morphological characterization of seeds, describing the various stages of post-seminal development, and to evaluate various conditions of temperature and substrate to perform the test germination. The seeds were manually extracted, then homogenized, where two samples of 50 seeds were used to determine the initial moisture. Another sample, consisting of eight repetitions of 100 seeds was used to measure the biometry and the number of seeds per fruit. The morphological characterization and the seeds were immersed in distilled water to allow the cuts to the observed structure in microscopes. In the post-seminal study, it was observed the daily processes of seedling growth in rolled paper filter and constant temperature of 30 °C. To assess the germination behavior, the constant temperatures of 15 °C, 25 °C, 30 °C, 40 °C and 20-30 °C as well as the paper and the sand substrates were tested and it was evaluated the percentage, the rate of germination, the relative frequency distribution, and the tests conducted in a randomized design in factorial 5 x 2 (x substrate temperature) with four replicates of 25 seeds, and the averages compared by Duncan 5 % probability. The fruits of black angico show great variation in the seed number per fruit. The embryonic axis occupies part of the central region of the seed with axial and linear positions. The germination is epigeal and the seedlings are fanerocotylar. The temperature determines the 30 °C and the filter paper substrate provided higher average percentage and germination rate.
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Were evaluated the economic effects of four grazing heights (20, 40, 60 and 80 cm) of Tanzânia grass for beef cattle. The total area was 12 acres divided into paddocks of one hectare, with three replications. We used three animals, males, Nelore per paddock, as more animals need additional adjustment to the height you want. The reduction in sward height allowed higher stocking rate, which, even with a reduction in individual performance, there was more beef production per area. The interest rate on capital given the property represented the largest share in the final cost of production. The participation of fixed variables showed a positive linear function of the increase in height from grazing, showing significant reduction of the production scale. A reduction in cost of production per hectare per year with increasing grazing height. No differences were found in certain economic indicators, and the four systems remunerate the capital invested.
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Sericiculture is characterized by the possibility of income generation in areas smaller than 10.0 ha and by the rationalization of family labor. Even considering this favorable scenario for the development of this activity, there is still a gap in the management of its economic factors in most of the rural properties within this chain, contributing negatively to their expansion. This study aimed at assessing the production cost and economic viability for producing silkworm cocoons, by using a case study conducted in a property located in Quatá, São Paulo State, Brazil. The main results for a production of 1,904 kg-1 of silkworm cocoons, with labor costs reaching 51.5% of the Effective Operating Cost and 35.5% of the Total Operating Cost, were a profitability index higher than 50% and an Internal Rate of Return of 9.68%, for the second production year, providing a good profitability rate for this agricultural segment, when we consider that the improved profitability of the producer is proportional to the best production indicators, particularly due to the fast return that this activity provides.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Engenharia Elétrica - FEIS
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)