Localização da proteína fosfolipase C zeta em extratos esppermáticos de gatos domésticos normospérmicos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparação ente dois métodos de inseminação artificial utilizando sêmem congelado em gatos domésticos(Felis catus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ocorrência do subtipo B do vírus da imunodeficiência felina em gatos domésticos da região sul do estado do Rio Grande do Sul, Brasil

In Brazil there are few studies on the occurrence of the feline immunodeficiency virus (FIV) infection and its subtypes, which are essential for the development of vaccines and new diagnostic tests. The present study investigated the occurrence of the FIV infection between 2010 and 2011 in domestic cats submitted to medical attendance in the city of Pelotas and nearby area. Total blood samples of seventy cats, suspected (28) or not (42) of infection by FIV were analyzed by nested PCR in order to perform a diagnosis. The results pointed to a FIV infection frequency of 15.7% (11/70) and the analysis of the risk factors related to infection (sex, age and clinical condition) evidenced a greater occurrence in cats up to 10 years of age with chronic and recurrent infections. Eight samples found positive by nested PCR were submitted to DNA sequencing indicating that only the subtype B was detected in the studied region.

Criptosporidiose em animais domésticos: aspectos epidemiológicos

Studies related cryptosporidiosis will be essential, due to its relevance in public health and pathogenicity in pets and production animals. Over the past 20 years, there has been a rapid expansion of research involving the Cryptosporidium genus, largely related to molecular studies, providing a description of various species, genotypes and subtypes of the parasite. The molecular characterization of isolates from different sources (human, animal and environmental) has been widely used in order to investigate the potential zoonotic of this protozoa. The documented transmission forms from animals to humans, from person to person, through water intake or water for the leisure that are directly or indirectly contaminated with sporulated oocysts. The high rate of animals naturally infected and the susceptibility by protozoan, justify the importance of attending to the occurrence of this disease. So are demonstrated epidemiological aspects of this zoonotic disease in domestic animals.

Uso de sêmen liofilizado de gatos domésticos (Felis silvestris catus) para produção in vitro de embriões através da técnica de injeção intracitoplasmática de espermatozóide (ICSI)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Platinosomíase em felinos domésticos: um diferencial para obstrução biliar

The platinosomiase is a disease the hepatobiliary system of domestic felines being caused by trematode Platynosomum fastosum. The life cycle of the parasite includes the presence of three intermediate hosts (snails, terrestrial isopodes, lizards or frogs). The cat is considered definitive host of the parasite and acquires the infection eating a vertebrate intermediate host containing metacercariae. The adult parasites generally inhabit the liver, gallbladder and bile ducts of cats. The severity of clinical manifestations depends mainly on the number of flukes in the biliary tract. Many animals are asymptomatic or exhibit nonspecific clinical manifestations such as anorexia, lethargy, weight loss, vomiting and diarrhea. In large infestations can occur biliary obstruction resulting in jaundice, cirrhosis, hepatitis cholangiohepatitis and even death. The definitive diagnosis is accomplished through by the detection of fluke eggs in feces or through laparotomy and liver biopsy. Treatment should be based on the use of anthelmintic for the elimination of the parasite and supportive therapy for the animal. Prevention is difficult due to predatory nature of the cat. The control can be accomplished through periodic fecal examinations

Retroviroses em felinos domésticos

The retrovirus are recognized as pathogenic group of virus for domestic animals. The particularitities of these viruses are the necessity of the enzyme transcriptase reversa, for the conversion of the viral RNA in viral DNA (provirus) and the incorporation in the DNA of the cell, what it confers to the infection the lifetime character, due to all the infected cells present the provirus our DNA. Among the retroviruses in domestic felines, the leukaemia and immunossupressive virus represent the more important diseases. The main form of transmission of the virus of the FeLV is occur by close contact and the saliva presents high viral concentration. For the FIV, the main form of transmission is represented by wounds of bite. The retrovírus, replicate mainly in high metabolization cells. The infection for FeLV cause mieloproliferativas and degenerative illnesses, while the FIV are related imunossupressora illness. The treatment for these retroviroses is symptomatic associated to imunomodulatory drugs, none of these drugs are capable to eliminate the virus. For the prevention of these retrovirus are used vaccines. However only the vaccine against FeLV have showed efficiency. Thus , the more important measures in control of these diseases is prevent the contact between infected and health felines. The ain of present study was reviewed the more important aspects of retroviruses in domestic felines, with emphasis to virulence properties, epidemiology, fisiopathogeny, clinical manifestations, methods of diagnosis, therapy, and control measures

Principais marcadores utilizados na avaliação da qualidade do sêmen de animais domésticos

Methods of semen cryopreservation allow changes in spermatic cells, such as damage in plasma and acrossomal membrane and modifications in mitochondrial function due to a disorder in the lipidic bilayer. For effective oocyte fertilization, spermatozoa require functional competent membranes, and intact organelles, acrosome and DNA. However, most laboratory methods used to evaluate semen quality are not highly correlated with fertilizing capacity. The discovery of a variety of fluorochromes and compounds conjugated to fluorescent probes has enabled an accurate assessment of the viability, integrity and function of spermatozoa. Among the most used probes that label the various compartments of the sperm cell there are the membrane impermeable fluorescent dyes to test the membrane integrity, as well as acylated dyes that pass the intact membrane. For the acrossomal integrity the most commonly used method is lectins labeled by a fluorescent probe. The acrosome reaction and spermatic capacitation is detected by the evaluation of membrane architecture and disorder of lipids in plasma membrane. Mitochondrial function can be determined using markers for their aerobic activity. The DNA status of spermatozoa has been determined using the metachromatic properties of Acridine Orange, and the DNA fragmentation can also be assessed by TUNEL assay. Finally, DNA condensation is analyzed using a single cell DNA gel electrophoresis assay that indicates DNA compactation. This monograph aims to compile the various tests used to detect damaged spermatozoa under cryopreservation methods, searching for improve the predictive value of semen analysis with the intention of a successful conception

Congelação de células espermáticas provenientes de epidídimo de gatos domésticos contendo antioxidante no meio diluidor

Pós-graduação em Medicina Veterinária - FCAV

Neuroimagem de encéfalo de gatos domésticos: correlação entre tomografia computadorizada e cortes anatômicos

Pós-graduação em Cirurgia Veterinária - FCAV

Detecção e caracterização de Escherichia coli potencialmente patogênicas em aves selvagens e pombos-domésticos na cidade de Jaboticabal-SP

Pós-graduação em Microbiologia Agropecuária - FCAV

Constrangimentos domésticos à política externa comercial dos Estados Unidos no governo Clinton (1993-2001)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Detecção do herpes vírus felino tipo 1 (HVF-1) pela técnica de PCR em tempo real e sua associação com sinais oculares em uma poipulação de gatos domésticos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da coenzima Q10 nos danos oxidativos induzidos pela L-tiroxina no músculo sóleo de ratos

Introduction: skeletal muscles are dynamic tissue that can change their phenotypic characteristics providing a better functional adaptation to different stimuli. L-thyroxine is a hormone produced by the thyroid gland and has been used as an experimental model for stimulation of oxidative stress in skeletal muscle. Coenzyme Q10 (CoQ10) is a fat-soluble provitamin endogenously synthesized and found naturally in foods such red meat, fish, cereals, broccoli and spinach. It has antioxidant properties and potential in the treatment of degenerative and neuromuscular diseases. Objective: to evaluate the protective effect of CoQ10 in the soleus muscle of rats against the oxidative damage caused by L-thyroxine. Methods: the rats were divided in four groups of six animals each: Group 1 (control); Group 2 (coenzyme Q10); Group 3 (L-thyroxine), and Group 4 coenzyme Q10 and L-thyroxine). After euthanasia, blood was collected and serum activity of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST) was analyzed. In the soleus muscle homogenates the factors related to oxidative stress were assessed. Results: CoQ10 protected the soleus muscle against the damage caused by L-thyroxine and favored the maintenance of the antioxidant enzymes glutathione reductase and glutathione peroxidase, the concentration of decreased and oxidized glutathione, and prevented lipid peroxidation. Conclusion: the results indicate that CoQ10 protects rat soleus muscle from oxidative damage caused by L-thyroxine.

Proposição de modelo de logística reversa de resíduos sólidos domésticos recicláveis para o municípios de Rio Claro - SP

The approval of the Solid Waste National Policy on August 2, 2010, brought important advances on the issue of solid waste nationwide. Several requirements were imposed causing public and private sectors to direct the problem more diligently. At the same time, the growth of human consumption and the negative impacts of related activities are increasing pressure for new ways to manage wastes. This study was carried out in order to bring proposals for a new model of solid waste management in the city of Rio Claro. To do so, it was used methodologies which stick to collaborative approaches in order to co-create a new vision considering different points of view . This new proposal includes four main initiatives: the integration of a reverse logistics system and collection of recycling materials in all kinds of educational institutions; the use of a routing platform for collection of solid wastes and recycling materials; the application of a garbage fee to residents who use the collection of solid waste system; and a bonus system developed by the private sector as a marketing strategy, encouraged by the public sector, enabling residents to separate their wastes correctly and leave them in one of the educational institutions to exchange points for discounts at local markets