Performance of Nellore young bulls on Marandu grass pasture with protein supplementation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of long-term treatment with insulin and/or acarbose on glomerular basement membrane thickening in alloxan-diabetic rats

Acarbose is a competitive inhibitor of the intestinal alpha-glycosidases, that can delay absorption of intestinal carbohydrates causing their malabsorption. In the present paper we studied the effects of insulin, acarbose and their association on glomerular basement membrane thickening in alloxan-diabetic rats. Twenty-five male and female Wistar rats, approximately 3 months old at the beginning of the experiment, were assigned randomly to each of five experimental groups: normal control rats, alloxan-diabetic control rats, alloxan-diabetic rats treated with acarbose, alloxan-diabetic rats treated with insulin, and alloxan-diabetic rats treated with insulin plus acarbose. Alloxan was administered in a single iv dose of 42 mg/kg body weight. Insulin was given subcutaneously at doses of 18 to 30 IU/kg corrected daily on the basis of glycosuria and ketonuria. Acarbose was given mixed with rat chow in a dose of 50 mg/100 g chow. Body weight, water and food intake and diuresis, as well as blood and urine glucose were determined after 1, 3, 6, 9, and 12 months of treatment. Glomerular basement membrane (GBM) thickening was determined by electron microscopy at the same times. Clear clinical and laboratory signs of severe diabetes, with blood glucose levels above 200 mg/dl and urine glucose above 3000 mg/dl, were observed in all alloxan-diabetic control rats, in all periods of follow-up, whereas administration of insulin or acarbose reduced the blood glucose levels of treated groups. The most satisfactory control of blood and urine glucose was observed in animals treated with both insulin and acarbose. However, diarrhea was observed in diabetic rats treated with acarbose associated or not with insulin. GBM thickening was correlated with age in all groups. Beginning at six months after diabetes induction, the GBM of untreated diabetic rats was significantly thicker (mean +/- SEM, 4.446 +/- 0.45 mm) than that of normal rats (2.977 +/- 0.63 mm). Both insulin and acarbose prevented GBM thickening and their combination induced thickening similar to the age-dependent thickening observed for normal rats of the same age. We conclude that acarbose when combined with insulin may be a good option in the control of diabetes and its renal complications.

Avaliação da Gravidade Específica e de Outras Medidas Corporais e da Carcaça para Estimar a Composição Corporal de Novilhos Nelore

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Task demand effects on postural control in older adults

The literature shows conflicting results regarding older adults' (OA) postural control performance. Differing task demands amongst scientific studies may contribute to such ambiguous results. Therefore, the purpose of this study was to examine the performance of postural control in older adults and the relationship between visual information and body sway as a function of task demands. Old and young adults (YA) maintained an upright stance on different bases of support (normal, tandem and reduced), both with and without vision, and both with and without room movement. In the more demanding tasks, the older adults displayed greater body sway than the younger adults and older adults were more influenced by the manipulation of the visual information due to the room movement. However, in the normal support condition, the influence of the moving room was similar for the two groups. These results suggest that task demand is an important aspect to consider when examining postural control in older adults. (c) 2006 Elsevier B.V. All rights reserved.

Crystallization and preliminary X-ray diffraction analysis of XAC1151, a small heat-shock protein from Xanthomonas axonopodis pv. citri belonging to the alpha-crystallin family

The hspA gene (XAC1151) from Xanthomonas axonopodis pv. citri encodes a protein of 158 amino acids that belongs to the small heat-shock protein ( sHSP) family of proteins. These proteins function as molecular chaperones by preventing protein aggregation. The protein was crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium phosphate. X-ray diffraction data were collected to 1.65 angstrom resolution using a synchrotron-radiation source. The crystal belongs to the rhombohedral space group R3, with unit-cell parameters a = b = 128.7, c = 55.3 angstrom. The crystal structure was solved by molecular-replacement methods. Structure refinement is in progress.

USE OF FALLEN COCOA PODS BY ANTS (HYMENOPTERA, FORMICIDAE) IN SOUTHEASTERN BRAZIL

Fallen cocoa pods serve as nesting and foraging sites for litter and ground ants in southeastern Brazil. The distribution of the number of species per pod deviated significantly from a Poisson distribution, with a prevalence of one species per pod. Only 17 species of a much larger fauna were present in pods, with the dominant species being Gnamptogenys striatula, and Oligomyrmex sp. Colony populations for 13 species are given.

THE INFLUENCE OF THE ELECTROCHEMICAL TREATMENT ON CU-AL-AG ALLOYS IN DEAERATED 0.5M NAOH

The effect of consecutive cyclic polarization in de-aerated 0.5 M NaOH solutions on the surface microstructure of mechanically polished Cu-Al-Ag alloys of different compositions and heat treatments has been studied using optical microscopy, SEM and EDS. The current peaks of the cyclic polarization curves do not depend on the alloy composition in the composition range studied. The repetitive potential scans between H2 and O2 evolution in alkaline media lead to preferential dissolution of aluminium, the roughness and phase composition of the surface of the alloys changing significantly. The quasistationary I-E curves of the different Cu-Al-Ag alloys studied consist in the superposition of the quasistationary I-E curves of high-purity Cu and Ag, the EDS microanalysis showing that aluminium is not present on the surface of the alloy in these conditions.

Excitation spectrum for ultrafast photogeneration of charged solitons in polyacetylene

Ultrafast photoinduced absorption by IRAV modes is used to detect charged solitons in oriented polyacetylene. We find that soliton pairs are photogenerated within our time resolution of similar to250 fs with similar to100% quantum efficiency (phi(ch)). The excitation spectrum of phi(ch) shows an onset at 1.0 eV, with a weak photon energy dependence up to 4.7 eV. These results agree with the ultrafast soliton formation predicted by Su and Schrieffer and with the SSH threshold of 2E(g)/pi for soliton pair production.

Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari : Ixodidae)

The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits. Rabbits were divided into 3 groups of 2 animals each: naive, pre-infested and control. Cutaneous hypersensitivity was induced by intradermal inoculation of 25 mug extract in 0.03 rut of phosphate buffered saline (PBS) in rabbit ears. Control rabbits were inoculated with PBS only. The ear thickness was measured with a Mitutoyo(R) device before and 10 min, 1, 2,4,18, 24,48,72 and 96 h post-inoculation (PI). Pre-infested rabbits showed an immediate type reaction within the 1st 10 min PI (60 % increase in ear thickness) and a delayed reaction (18 h) (85 % increase), whereas the naive rabbits showed only the immediate reaction within the 1st 4 h (60 % increase). PBS induced only mild reactions. These results point out the crucial role of the cellular immune response of rabbits in the expression of resistance to A. cajennense.

Design, manufacturing, and testing of a Nb3Sn coil employing Ti alloy as structural material

We describe the design, manufacturing, and testing results of a Nb3Sn superconducting coil in which TiAIV alloys were used instead of stainless steel to reduce the magnetization contribution caused by the heat treatment for the A-15 Nb-3 Sn phase formation that affects the magnetic field homogeneity. Prior to the coil manufacturing several structural materials were studied and evaluated in terms of their mechanical and magnetic properties in as-worked, welded, and heat-treated conditions. The manufacturing process employed the wind-and-react technique followed by vacuum-pressure impregnation(VPI) at 1 MPa atm. The critical steps of the manufacturing process, besides the heat treatment and impregnation, are the wire splicing and joint manufacturing in which copper posts supported by Si3N4 ceramic were used. The coil was tested with and without a background NbTi coil and the results have shown performance exceeding the design quench current confirming the successful coil construction.

Desempenho em pastagens e características de carcaça da 16a progênie dos rebanhos Nelore, Guzerá e Caracu de Sertãozinho (sp)

Forty one young bulls of herds selected for 378 day's weight (W378), born in 1996, were finished on pastures of Panicum Maximum (Jacq.), Panicum Maximum (Jaq) cv. Tanzania 1 and Brachiaria brizantha (Hoschst) Stapf cv. Marandu at the Sertaozinho Experimental Station, São Paulo State, Brazil. The samples, representing the W378 mean for each herd, were composed by 11 Nellore Selection (NeS) and by 10 of each one of the groups Nelore Control (NeC), Guzera Selection (GuS) and Caracu (Ca). The slaughter was carried out when the animals were 824 days older, with a body condition score averaging 7.6, in a 1-9 scale. The minimum and maximum adjusted means for the main traits, including all groups, were: average weight daily gain, 406 (NeC) and 501 g (NeS); slaughter weight (SW), 446.8 (NeC) and 544.3 kg (NeS); carcass weight (CW), 249.8 (NeC) and 309.7 kg (NeS); dressing percentage (DP), 54.0 (GuS) and 56.3% (NeC and NeS). In the 9(th) - 11(th) rib section: muscle, 59.6 (NeC) and 65.2% (Ca); fat, 15.6 (Ca) and 21.4% (NeC); bone, 18.9 (NeC) and 20.2% (GuS); fat thickness (FT), 2.0 (Ca) and 4.2 mm (NeC); loin eye area, 65.6 (NeC) and 71.1 cm(2) (NeS and Ca); Warner-Bratzler shear force (SF), 4.5 (Ca) and 6.6 kg (GuS) and total cooking losses (TCL), 22.5 (NeC) and 24.9% (GuS). The selection for weight promoted higher SW and CW in the NeS group, without changing the DP, the physical composition of the rib, SF and TCL in the meat. However, there was lower FT compared to NeC. The GuS animals had intermediates SW and CW, compared to NeS and Ca and lower DP. The Ca animals presented higher muscle percentage, in the rib section, and also higher meat tenderness compared to the meat of the Zebu animals.

Effects of the eccentricity on the primaries in the gravitational capture phenomenon

Gravitational capture is a characteristic of some dynamical systems in celestial mechanics, as in the elliptic restricted three-body problem that is considered in this paper. The basic idea is that a spacecraft (or any particle with negligible mass) can change a hyperbolic orbit with a small positive energy around a celestial body into an elliptic orbit with a small negative energy without the use of any propulsive system. The force responsible for this modification in the orbit of the spacecraft is the gravitational force of the third body involved in the dynamics. In this way, this force is used as a zero cost control, equivalent to a continuous thrust applied in the spacecraft. One of the most important applications of this property is the construction of trajectories to the Moon. The objective of the present paper is to study in some detail the effects of the eccentricity of the primaries in this maneuver.

Effect of passive pneumoperitoneum on oesophageal pressure, cardiovascular parameters and blood gas analysis in horses

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparação entre a adiposidade subcutânea dos hemicorpos direito e esquerdo

O objetivo deste estudo foi comparar os níveis de adiposidade subcutânea dos hemicorpos direito e esquerdo e, posteriormente, analisar o impacto dessas informações para o estudo da composição corporal. Setenta e seis indivíduos fisicamente ativos, 47 homens (21,6 ± 4,3 anos) e 29 mulheres (21,0 ± 2,6 anos), fizeram parte da amostra. As espessuras das dobras cutâneas abdominal, suprailíaca, subescapular, tricipital, bicipital, axilar média e perna medial foram mensuradas com um compasso Lange. Em valores médios absolutos, as maiores diferenças verificadas foram de 0,9mm (6,9%) e 0,8mm (6,8%), na dobra cutânea suprailíaca de homens e mulheres, respectivamente. Entretanto, nenhuma diferença estatisticamente significante foi encontrada na comparação entre os lados, em ambos os sexos, nas sete dobras cutâneas analisadas (P > 0,05). Similarmente, quando os valores medidos foram aplicados em equações preditivas para a determinação da gordura corporal relativa, de acordo com o sexo, nenhuma diferença significante foi encontrada (P > 0,05). Os resultados sugerem que fatores como o erro técnico de medida do avaliador, o tipo de compasso e a escolha da equação preditiva a ser utilizada, provavelmente tenham maior impacto para a estimativa da composição corporal pelo método de espessura de dobras cutâneas do que o lado a ser adotado como referência para a obtenção das medidas.