Estado físico do meio de cultura na propagação in vitro de bromeliaceae

The objective of this study was to evaluate the effect of the physical state of the culture medium on the behavior in vitro of the bromeliaceas Neoregelia cruenta, Tillandsia stricta, Vriesea gigantea, V. guttata e V. incun/ata. Micropropagated shoots had been cultivated in culture medium MS, with 30 g sucrose, 2,5 mg dm-3 BAP, and 0,5 mg dm-3 ANA, establishing the treatments: T1- half-solid with 7 g agar; T2- static liquid; T3- liquid under-agitation of 90 rpm; and T4-static liquid with bridge of paper filter. After 30 days, the use of static way of liquid culture presented better results in relation of proliferative average rate in all the species (9.4 shoots in N. cruenta, 5.6 in T. stricta, 11.5 in V. gigantea, 9.2 in V. guttata and 3.9 in V. incurvata). In relation the average height of the shoots, was distinguished for N. cruenta the liquid under-agitation (2.83 cm), T. stricta and V. incurvata the semisolid (1.76 cm and 2.02 cm, respectively) and V. gigantea and V. guttata the static liquid (0.61 cm and 1.48 cm, respectively). The use of medium MS static liquid was more suitable for in vitro culture of bromeliads species studied.

Sementes de feijão-caupi (Vigna unguiculata L. (Walp), tratadas com extrato de folhas de nim (Azadirachta indica A. Juss.) avaliação da germinação e da incidência de fungos

This study aimed to evaluate the effect of aqueous extract of neem on germination and fungi incidence on seeds of three cultivars (Serrinha, BR 17 and Maranhão) of cowpea. Neem leaves were dryed, crushed and prepared dilutions of 0.5; 1.0; 2.0, 4.0 g dm -3and control. The fungi incidence was evaluated by the test filter paper and germination according to the Rules for Seeds Testing (Regras para Análise de Sementes). In the three cultivars analyzed, reduction in the incidence of Aspergillus sp and Fusarium sp was observed. In relation to the influence of extracts of neem leaves on seed germination, significant effect of extract in Maranhão cultivar was observed, where all concentrations differed from the control, and propovided a considerable increase in the percentage of normal seedlings. It was concluded that the leaf extract of neem was effective in controlling Aspergillus sp, Fusarium sp , Phoma sp and Macrophomina phaseolina at different concentrations in different cultivars and seed germination was stimulated for the Maranhão cultivar.

Nota científica: Clonagem do abieiro por estaquia herbácea de ramos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Emergência de plantas daninhas em função da posição da semente e quantidade de palha de cana-de- açúcar

This work was carried out to evaluate the effects of sugarcane straw density and positions of seeds of Euphorbia heterophylla, Mucuna cinereum, Digitaria nuda and Eleusine indica on emergence and initial development of plants. The experiment were arranged in a completely randomized design, with 15 treatments and four replications, in a factorial arrangement 3 x 5 with a additional control; the first factor was constituted by position of seeds related to the straw (under, between and on) and the second by five quantities of straw (1, 2, 4, 8 e 16 t ha -1) with a control without straw. At 15 days after sowing (DAS) it was realized evaluations of height, dry mass and emergence percentage for E. heterophylla and M. cinereum, and at 28 DAS it was analyzed dry mass and emergence percentage for D nuda e E. indica. Data obtained were submitted to F in variance analysis test and means were compared with Tukey at 5% probability. Emergence and initial development of E. heterophylla e M. cinereum were reduced with increasing of straw and were affected by seed position, but it was not inhibited in none of conditions, indicating aggressiveness of these weeds in sugarcane mechanized harvest conditions. D. nuda and E. indica emergence was not affected by seed position and coverage soil with 8 e 16 t ha -1 of sugarcane straw inhibited its emergence.

Capacidade predatória de chrysoperla externa sobre bemisia tabaci biótipo b expostas ao fungo metarhizium anisopliae

The objective of this study was to determine the influence of Metarhizium anisopliae on the predatory capacity and functional response of Chrysoperla externa fed Bemisia tabaci B biotype rearing cotton. The stock rearings of B. tabaci B biotype and C. externa were maintained in cages according to preexisting methodologies. Upon hatching, predator larvae were fed on eggs of Ephestia küehniella until their 3rd instar and then fed on whitefly nymphs in density exceeding their intake capacity (150 nymphs/individual). The nymphs were offered on cotton leaf disks (5 cm diameter), 24 hours after the treatments application following: T1: Control (water); T2: M. anisopliae at the concentration of 105 conidia cm-3; T3: 10 8 conidia cm-3 origined from Metharril® formulation of the 109 viable conidia cm-3 M. anisopliae active principle. The predatory capacity was obtained by the difference between the numbers of nymphs supplied and nymphs left. Five different nymphal densities (130, 160, 190, 220, and 250) were selected for the functional response study. The number of nymphs preyed was recorded after 24, 48, 72, and 96 hours. The M. anisopliae entomopathogenic fungi did not influence predatory capacity of C. externa which ranged from 70.6 individuals at the highest concentration to 87.1 in the control. Conclued that the C. externa predatory capacity was not affected by fungi M. anisopliae concentrations. The predator showed a type-II functional response after 24 hours, and a type-I response after 48, 72s, and 96 hours independent from fungi concentrations.

Fecal progestins during pregnancy and postpartum periods of captive red brocket deer (Mazama americana)

This study aimed to validate the enzyme immunoassay (EIA) for fecal progestin quantification of the species Mazama americana, define its excretion profile during periods of gestation and postpartum and determine the gestation period and resumption of postpartum ovarian activity in this species in captivity Fecal samples were collected twice a week during gestation and every day in the postpartum period, and analyzed using EIA The mean concentrations (±SEM) of fecal progestins during gestation were 2180.0 ± 299.1 ng/g in early pregnancy (week 1-11), 3271.4 ± 406.9 ng/g in middle pregnancy (week 12-22) and 5592.0 ± 1125.8 ng/g in late pregnancy (week 23-32) The gestation period determined for the species was 220.9 ± 1.2 days The concentration of progestins reached its peak prior to parturition and returned to baseline levels in 4 ± 0.31 days after parturition In the postpartum period, the mean concentrations of fecal progestins were 1564.2 ± 182.6 ng/g in the interval between parturition and resumption of ovarian activity, 469.8 ± 24.5 ng/g in the inter-luteal phase and 2401.7 ± 318.5 ng/g during the luteal phase, such that the postpartum period and the luteal phase differed from the inter-luteal phase Fecal progestin profiling permitted the detection of ovulation 26.9 ± 3.4 days after parturition in all the hinds studied and estimation of the mean duration of the estrous cycle, 21.3 ± 1.1 days Analysis established that concentrations of progestins above 3038.76 ng/g diagnosed pregnancy, a value determined from the week 12 of gestation Moreover, the quantification of fecal progestins by EIA proved to be an important tool for noninvasive endocrine monitoring and to obtain reproductive data on the species M americana in captivity © 2013 Elsevier B.V.

Visceral colouration of Eupemphix nattereri (Anura: Leiuperidae): effects of NDP-α-melanocyte stimulating hormone

Amphibians have melanin-containing cells in visceral organs that are similar to pigmentary cells from the epidermis. Both of them are derived from the ectodermal neural crest. Epidermal cells respond to α-melanocyte stimulating hormone (α-MSH), which is associated to the dispersion of melanin granules within melanocytes. Therefore, our aim was to test whether a non-degradable analogue of the α-MSH changes the superficial colouration of organs of Eupemphix nattereri. The hormone rapidly increases (within 12 hours) the colouration on the surface of the pericardium, heart, testes, nerves of the lumbar plexus, and lumbosacral parietal peritoneum. Colouration increased late (after 24 hours) in the kidneys and mesentery following hormone administration. However, this hormone did not change colouration of intestine, rectum and lungs. Our findings could be explained by the similarities between epidermal and visceral melanocytes, since both cells have a common embryonic origin. Furthermore, the increase in visceral colouration may be related to the dispersion of melanosomes within melanocytes, which causes the darkening of organs. Our results demonstrate for the first time that the visceral colouration is responsive, thereby altering the internal pattern of organs' colouration in anurans. © 2013 Copyright 2013 Unione Zoologica Italiana.

Parâmetros genéticos entre caracteres quantitativos no girassol como critério de seleção para produtividade de aquênios

Two experiments were conducted in southern Brazil in no-tillage system, in order to estimate genetic parameters and the direct and indirect effects of components for achene yield as a selection criterion in sunflower. We analyzed eight sunflower hybrids at two locations, in a randomized complete block design with four replicates, determined through quantitative descriptors: insertion height of the head, and head stem diameter, weight of 1000 achenes, number of achenes per head, mass by achene head and yield achene. Estimates of genetic parameters were based on combined analysis, decomposing interactions in genetic and environmental components. Considering the coefficient of genetic variation, indirect effects of components and heritability, there are higher possibilities for responses to selection in sunflower achenes by descriptors mass and mass of achenes per head, with its indirect association interrelated pathways for the increase in the achenes of yield.

Efeito dos solutos urêmicos sobre espécies reativas de oxigênio em sistemas-modelo in vitro

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Pseudoplatystoma metaense and P. orinocoense (Siluriformes: Pimelodidae) from the Orinoco basin, Venezuela: cytogenetic and molecular analyses

The genus Pseudoplatystoma includes catfish species distributed throughout the fresh waters of South America. These species are important fisheries resources and play a significant ecological role due to their piscivorous and migratory habits. The taxonomy of this genus is still debated: traditionally, only three species have been recognised, but recently, this number was raised to eight. The validity of these eight morphospecies, however, was not confirmed by two subsequent molecular phylogenetic studies, which identified either five or four main clades. In this study, we focused on the two morphospecies restricted to the Orinoco basin, P. metaense and P. orinocoense, which have been assigned to either the same or different clades in previous studies. We carried out cytogenetic analyses to describe their unknown karyotypes and to look for cytotaxonomic markers. We also analysed their mitochondrial sequences in order to assign the sampled specimens to the previously identified molecular clades. The two presumptive species show similar karyotypes (2n=56, 42 biarmed and 14 uniarmed chromosomes) and cytogenetic features in terms of the constitutive heterochromatin distribution and the number and location of minor and major ribosomal genes. Thus, no species-specific chromosome markers could be identified. The analysis of cytochrome b and cytochrome oxidase I mitochondrial genes (carried out by retrieving all the mtDNA Pseudoplatystoma sequences available in GenBank) distributed the sampled specimens into two distinct molecular clades and confirmed the need to re-evaluate, by parallel morphological and molecular analyses, the monophyly of some lineages.

Bone Marrow'S Harvest in The Coxal Tuberosity for Isolation and Culture of Mesenchymal Stem Cells of Buffaloes (Bubalus Bubalis)

Several studies with mesenchymal stem cells (MSCs) have been developed in many species because of its ability to differentiate into other mesoderm lineages, capacity of self-regeneration, low immunogenicity, paracrine, anti-inflamatory, immunomodulatory and antiapoptotic effects which make then a promissory source to be used in therapeutic strategies. The aim of this study is to report the technique of harvest of bone marrow (BM) in the coxal tuberosity (CT) of buffaloes. For this, the animals were selected, identified and contained in a stock. Then trichotomy was performed in the region corresponding to the CT. After identifying the anatomic site it was performed antisepsis, local anesthetic block and introduction of a myelogram's needle (Lang(R)) for BM aspiration. Once the needle was firmly fixed in the CT, the mandril was removed and proceeded to BM aspiration with a syringe (20 mL) containing 1 ml of heparin at 1000 IU / mL and 1 mL of PBS. After the collection, each sample collected was manually homogenized, identified and referred to the LRACT - FMVZ / UNESP-BRAZIL for the correct processing. The anatomical site tested showed to be an alternative site of harvest of BM once provided the appropriate isolation and culture of the mononuclear fraction. Moreover, the procedure was performed without difficulty and with great security. Based on this, it can be conclude that CT is an excellent anatomical site for isolation and culture of MSCs and the proposed technique is viable and feasible to be held in buffaloes.

Cytotoxic, biochemical and genotoxic effects of biodiesel produced by different routes on ZFL cell line

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Cimetidine Reduces Alveolar Bone Loss in Induced Periodontitis in Rat Molars

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação das alterações dentárias e esqueléticas induzidas pelo uso do aparelho de Herbst no tratamento da classe II na dentadura mista: Revisão sistemática

Objective: evaluate the immediate dental and skeletal changes induced by the Herbst appliance on early treatment of Angle´s Class II malocclusion. Material and Method: several electronic databases such as Scopus, Pub Med, Medline, Cochrane Library, Lilacs and Scielo were searched. The abstracts that fullfilled the selection criteria were selected and those that did not provide enough information about these criteria were selected but the final decision of including them or not on the research, was taken after the complete reading of the article. The selection criteria were: clinical studies with Class II individuals, both male and female, with initial age of 7 to 10 years, treated with the Herbst appliance that analysed the dental and/or skeletal changes evaluated on lateral cephalometric radiographs; researches where the treatment performed did not involve extractions or surgical interventions; studies that included patients without syndromes or health concerns and articles published in English or Portuguese. Results and Conclusions: five articles were selected. The articles showed that significant changes happened in the mandibular sagittal lenght, on facial convexity angle, on maxillo-mandibular relationship, on retroclination of the upper incisors and on distal movement of the upper molars, on proclination of the lower incisors and on extrusion of the lower molars. The appliance exerted a limited effect on the anterior relocation of the maxillary complex and on facial heights. However, more studies about the performance of the Herbst appliance on early treatment of Class II are needed.