Análise da espermatogênese e do cariótipo da espécie Eptesicus furinalis (d’Orbigny, 1847) Vespertilionidae, Chiroptera

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da integração de esferas de hidroxiapatita sintética e de polietileno poroso em cavidades evisceradas de coelhos

Para a recuperação da estética facial,há necessidade de reposição do volume orbitário perdido em decorrência de eviscerações ou enucleações. Este estudo foi realizado com os objetivos de avaliar comparativamente a biocompatibilidade e a manutenção do volume orbitário com o uso de esferas de hidroxiapatita sintética e de polietileno poroso, na reconstrução de cavidades evisceradas de coelhos. Métodos: Para isso foram utilizados 56 coelhos albinos, submetidos à evisceração do olho direito, com colocação de esferas de hidroxiapatita sintética (G1 – 28 animais) ou polietileno poroso (G2 – 28 animais).Quatro coelhos de cada grupo foram sacrificados em 7 momentos experimentais: 7, 15, 30, 60, 90, 120 e 180 dias após a evisceração. Após o sacrifício, o conteúdo da cavidade orbitária direita foi removido e seccionado na porção central em duas hemi-metades,uma das quais foi preparada para exame histopatológico; exame ultra-estrutural em microscópio eletrônico de varredura foi feito em dois animais de 7, 60 e 180 dias, de ambos os grupos experimentais. Resumo 118 Resultados: Observou-se aos 7 dias, tecido conjuntivo frouxo, constituído de células inflamatórias e hemáceas, em meio à rede de fibrina, principalmente na periferia da esfera de hidroxiapatita e chegando até ao centro da esfera de polietileno; a reação tecidual tornou-se mais densa com o passar do tempo. Decorridos 60 dias, observou-se em G1, início de metaplasia óssea que se acentuou ao longo do experimento. Em todos os momentos experimentais, a inflamação,predominantemente macrofágica, foi muito mais acentuada em G1.O volume da cavidade esclero-corneana foi melhor mantido em G2. Conclusão: a esfera de hidroxiapatita sintética provoca mais inflamação que a de polietileno poroso e é menos eficiente na manutenção do volume orbitário... (Resumo completo, clicar acesso eletrônico abaixo)

THE PARASITOID, Cotesia flavipes (CAMERON) (HYMENOPTERA: BRACONIDAE), INFLUENCES FOOD CONSUMPTION AND UTILIZATION BY LARVAL Diatraea saccharalis (F.) (LEPIDOPTERA: CRAMBIDAE)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morphology and histochemistry of the digestive tract in carnivorous freshwater Hemisorubim platyrhynchos (Siluriformes: Pimelodidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Labral gland in soldiers of the neotropical termite Cornitermes cumulans (Isoptera: Termitidae: Syntermitinae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Development and evolution of the gynoecium in Myrteae (Myrtaceae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Long-Term Spinal Ventral Root Reimplantation, but not Bone Marrow Mononuclear Cell Treatment, Positively Influences Ultrastructural Synapse Recovery and Motor Axonal Regrowth

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análises morfológica e histoquímicas do ventrículo e do cérebro de operárias recém emergidas de Apis Mellifera L. (Hymenoptera, Apidae) tratadas com Thiamethoxam

Apis mellifera honeybees are social insects of economic importance, by providing honeybee products, and by the pollination of natural areas of vegetation or agricultural areas. The constant use of pesticides, including the thiamethoxam, which is an insecticide belonging to the class of neonicotinoids with neurotoxic action, is subjecting pollinators to situations of severe stress, which has been evidenced by the decrease in the density of honeybees in many parts of the world. By these considerations, the present study aimed to assess the acute toxicity of thiamethoxam for newly emerged workers of A. mellifera, and to investigate the effect of sublethal doses of this insecticide on the survival time and its cytotoxicity to the brain and midgut to the honeybees. It was established the value of the lethal concentration 50 (LC50) equal to 4.28 ng thiamethoxam/μL of food and from it, the sublethal concentrations of 0.428 ng thiamethoxam/μL (CL50/10) and 0.0428 ng thiamethoxam/μL (CL50/100), which were used in bioassays of intoxication of the honeybees. After the bioassays the bees were dissected and the brain and midguts were collected to analyze possible morphological (staining with Hematoxylin-Eosin) and histochemical alterations (Xylidine Ponceau technique, and Feulgen and PAS reactions) caused by exposure to thiamethoxam, and to calculate the lethal time (LT50) for the workers. The duration of the bioassays was 8 days after beginning of feeding. The results obtained showed that the thiamethoxam is toxic to newly-emerged workers of A. mellifera, causing changes in survival time of individuals. This study also shown that the thiamethoxam causes morphological and histochemical alterations on the midgut and brain of workers. These alterations may be reflected in physiological and behavioral changes that can modify the operation of the colony

Tubo digestório de Urostreptus atrobunneus (Diplopoda): estrutura e função

Millipedes are macro-arthropods of soil which perform a important role in maintaining the balance of the ecosystem, since they feed on decaying organic matter and because they promotes the soil enrichment, aeration and humidification. These animals are usually found in humid with low light places, under rocks and trunks. The diplopods exoskeleton is much resistant and impregnated with calcium salts. Due the secretion of defense, with strong and unpleasant smell, the animals of this class have few or no predators. Large diplopods populations are not observed, but explosions population can occur for unbalances enviromnmental, by climate change and by use of pesticides that may eliminate potential competitors. In this sense, the millipede Urostreptus atrobrunneus Pierozzi & Fontanetti, 2006, has presented points of infestation in urban centers of Sao Paulo state, causing many disorders of the human population. Because it is a newly described species, little is known of its aspects biological and morphological. Thus, the purpose of this study was to describe the structure and function of the digestive tract of the species U. atrobrunneus by means of histological, histochemistry and ultrastructural techniques. The results demonstrated that the digestive tract of U. atrobrunneus is similar to the descriptions given for other species. The foregut consists of a simple epithelium, composed of cells of different heights, covered by a cuticular intima, leaning on a thin basal membrane, followed by a well-developed muscular layer and an external membrane, around the foregut have a pair of salivary glands. The midgut epithelium has a pseudostratified, supported by a thick basal membrane, followed by a muscular layer and a layer of hepatic cells. The muscle is distributed unevenly coated with an external membrane, this portion is marked by the absence of cuticular intima... (Complete abstract click electronic access below)

Study of the ventral prostate of Wistar rats treated with Heteropterys tomentosa (A. Juss.)

Heteropterys tomentosa is a Brazilian plant traditionally used as an aphrodisiac and stimulant. Previous studies suggested possible androgenic and antioxidant effects after long term administration of H. tomentosa infusion. The aim of the present study was to evaluate the effects of this plant infusion on the rat ventral prostate: an androgen responsive organ. Wistar rats were treated, by gavage, with H. tomentosa roots infusion (treated group, n=6) or water (control group, n=6) for 56 days. Morphological, morphometrical and stereological analyses were employed to study the ventral prostate tissue, as well as androgen receptor and apoptotic cell staining. The ultrastructure of the prostatic epithelium was also analyzed. No alteration was observed in the stereological and morphometrical analyses. The pattern of androgen receptor expression and the apoptotic index were identical in the control and treated group. Ultrastructural analysis showed no alterations caused by H. tomentosa. These results suggested that treatment with H. tomentosa infusion, although considered a strong aphrodisiac, did not cause any major damage nor benefit to the prostate tissue.

Efeitos da congelação lenta e da vitrificação na viabilidade, ultraestrutura e expressão gênica de embriões caninos produzidos in vivo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação de proteínas Vip3A e Cry1la10 de Bacillus thuringiensis com atividade inseticida a lepidópteros-praga

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Toxicidade e interação de proteínas Cry1 de Bacillus thuringiensis em Helicoverpa armigera (Hübner) (Lepidóptera: Noctuidae)

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Avaliação dos efeitos de concentrações subletais de dimetoato no cérebro e ventrículo de Apis mellifera africanizada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)