562 resultados para Analise e comparação filogenética


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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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The use of pesticides to manage pest problems in agriculture has become a common practice around the world. Pesticides consist in chemical substances or biological agents that act preventing, destroying, repelling or mitigating the damage caused by pests in agriculture. In order to a successful treatment, the correct dosage of pesticide has to be applied for each kind of culture. In this context, this project aims to quantify the levels of the active ingredients Imidacloprid and Chlorpyrifos present in formulated technical products and compare with the reported in the ir commercial leaflets. The analyzes were performed by Ultra Performance Liquid Chromatography coupled to a photodiode array detector (CLUE-DAD). The identification of Imidacloprid and Chlorpyrifos in the samples was realized by comparison of the time of retention and UV spectre of those substances with the commercials patterns, resulting in a excellent method specificity of the method. In the scope of determination of active ingredients in formulated products, the method developed showed good sensitivity in the applied conditions, with suitables limits of detection (4,2.10-² mg L-¹ for Imidacloprid and 1,1.10-¹ mg L-¹ to Chlorpyrifos) and quantitation (1,4.10-¹ mg L-¹ and Imidacloprid 3,6.10-¹ mg L-¹ to Chlorpyrifos) for the concentrations found in matrices analyzed. Furthermore, good results were obtained to the method accuracy with coeficiente of variance values in the range of 0,6 to 0,7% for repeatability (intra-day) and 0,4 to 0,58% for intermediate precision (inter- day). The accuracy of the method was determined by recovery tests, the values were comprised in the range of 98 102%. According to the recommendations of the Associação Brasileira de Normas Técnicas, these values show good accuracy of the method. The concentrations obtained in the quantification of the active ingredients in formulated products showed values within permited by ABNT, 60.1% (w/v) ± 0.6%...

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Tradicionalmente a prescrição de exercícios físicos é baseada unicamente em relação à carga externa. Entretanto, as adaptações fisiológicas em função do treinamento físico dependem da carga interna. Assim, a carga interna deve ser levada em consideração para uma otimização da prescrição exercícios físicos, principalmente durante exercícios em grupos que normalmente são utilizados para a população idosa. Porém, o envelhecimento parece acarretar em deteriorações na capacidade de interocepção, colocando em questionamento a validade de um dos principais métodos de quantificação da carga interna, o método que utiliza a percepção subjetiva de esforço (PSE). Desse modo, o objetivo do presente estudo foi comparar os métodos de quantificação da carga interna que utilizam a frequência cardíaca (FC), considerado o padrão-ouro para o presente estudo, com o método que utiliza a PSE na população idosa. Quarenta idosos praticantes de ginástica aeróbia (A) ou musculação (M) foram avaliados durante uma sessão de exercícios físicos. A determinação da carga interna pela FC e pela PSE foram realizadas através dos métodos de Edwards e de Foster, respectivamente. Os valores de carga interna (expressos em unidades arbitrárias) calculados pelo método da FC e PSE foram 69,4 ± 40,0 e 151,5 ± 83,5 para o grupo M e 73,3 ± 37,1 e 134,7 ± 41,6 para o grupo A, respectivamente. O valor de carga interna para os dois grupos em conjunto foi de 71,3 ± 38,2 pelo método da FC e de 143,1 ± 65,7 pela PSE. A relação entre os métodos foi determinada usando-se o coeficiente de correlação de Pearson para todos os participantes em conjunto (T), assim como para os grupos M e A. As correlações encontradas foram de r = 0,33 (p < 0,05) para T, r = 0,37 (p = 0,11) para M e r = 0,32 (p = 0,17) para A. A moderada correlação obtida coloca em questão a utilização do método da PSE para a quantificação de carga interna...

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Pós-graduação em Agronomia (Ciência do Solo) - FCAV

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The identification of Salmonella spp. in food samples by microbiological diagnosis is time consuming, with approximately five different stages, requiring about 120 hours until the final result. The utilization of the polymerase chain reaction technique (PCR) can reduce this time, but substances present in samples may affect the reaction. The present work aimed to compare DNA extraction by thermic treatment and by the use of cetyltrimethil ammonium bromide (CTAB), in products originated from poultry houses corresponding to raw material (meat meal) and experimentally contaminated drag swabs. Materials obtained from the extractions were submitted to PCR, utilizing a pair of initiator oligonucleotides for amplification of Sdf 1 gene fragments. Comparing the methods of extraction, it was observed that when CTAB was employed, SE was detected in 70% of meat meal and in 80% of drag swabs, while the thermic treatment method yielded positive results in 20% of meat meal and in 40% of drag swabs. SE was detected under both methods utilized for DNA extraction, but the use of CTAB detected a greater number of positive samples, compared with thermal treatment.

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In this study, we compared the production cost of summer corn growing in soils treated with industrial fertilizer with those grown treated with sewage sludge. Corn was cultivated on reduced tillage treatment during 2002/2003 in an Oxisol area of 0.0625 hec. The field trial with industrial fertilizer was conducted following related guidelines for the state of Sao Paulo. The application of sewage sludge to the soil was at the rate of 10 t.ha-1. Calculations were based on market prices for the abovementioned years. In addition to lower environmental impact, results indicate economic cost advantages associated with the use of sewage sludge.

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The great importance of citrus in São Paulo state, particularly in Botucatu city, the and its major expansion in area in recent years and the advancement of techniques for environmental monitoring, such as remote sensing motivated the present research. The aim of this study was the determination the areas and perimeters of citrus in the Botucatu city, São Paulo state, Brazil, obtained through the softwares: Carta Linx, GIS-Idrisi and SPLAN, using aerial colored photographs of 2005, scaled at 1:30000 with longitudinal covering of approximately 60% and 30% laterally. The areas obtained from the aerial photographs and evaluated by Carta Linx presented values closer to the terrestrial reality in determining areas and perimeters, whereas GIS-Idrisi showed discrepancies in the determination of the perimeters, due to working with matrixes. SPLAN and Carta Linx had values for perimeters and areas in close proximity, due to work with vectors.

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Biodiesel is a reality in Brazil, due to the National Program of Biodiesel Production and Use (PNPB), which became mandatory in 2008 in adding 2% biodiesel in all diesel sold in a blend known as B2. The agribusiness sector responded to the federal program, facing many difficulties in biodiesel production, which highlights the supply of raw material. Problems related to biodiesel production were mainly the shortage of vegetable oil, because of demand from domestic and foreign markets, and rising prices of raw materials available for production, making this biofuel production economically feasible, even in Brazil, where agricultural production costs are among the most competitive in the world. The crambe is a specie that has aroused the interest of Brazilian producers due to oil content, hardiness, and mechanized cultivation, mainly for being a winter crop, it becomes one more option for farmers in this period. In addition, you can compose systems of crop rotation as well as being used as ground cover in winter. The aim of this study was to evaluate the potential for productive and cost of production of crambe, conducted in no-tillage system, to compare these parameters with other oil crops such as sunflower, canola and soybean. The trial was conducted in the agricultural year 2008 at the Experimental Farm Lageado belonging to the Faculty of Agronomic Sciences - UNESP, located in Botucatu - SP. The estimated yield was 1.507,05 kg ha-1, resulting in a production of 561,94 liters of oil per hectare. The cost of installation and conduct of crambe per hectare was R$ 875,87, resulting in a cost of R$ 1,56 per liter of oil, the lowest cost among the oilseed crops analyzed.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Pós-graduação em Biopatologia Bucal - ICT

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB