MS222 does not induce primary DNA damage in fish

The genotoxicity of the anaesthetic MS222 (tricaine) was analysed in fish under both in vivo and in vitro conditions. Based on results of the single cell gel/Comet assay, MS222 had no direct genotoxic effect on the experimental fish, indicating that MS222 does not induce primary DNA damage. These results suggest that the use of this important anaesthetic in aquaculture can be considered to be safe in terms of genotoxicity. © Springer Science+Business Media, Inc. 2007.

Analysis of DNA damage induced by aflatoxin B1 in Dunkin-Hartley guinea pigs

Aflatoxin B1 (AFB1) is among the most potent naturally occurring carcinogens and classified as a group I carcinogen. Since the ingestion of aflatoxin-contaminated food is associated with several liver diseases, the aim of the present study was to evaluate the effect of 2, 20, and 200 ppb of AFB1 on DNA damage in peripheral blood lymphocytes and liver cells in Dunkin-Hartley guinea pigs. The animals were divided into four groups according to the given diet. After the treatment the lymphocytes and liver cells were isolated and DNA damage determined by Comet assay. The levels of DNA damage in lymphocytes were higher animals treated with 200 ppb of AFB1-enriched diet (P = 0.02). In the liver cells there were a relationship between the levels of DNA damage and the consumption of AFB1 in all studied groups. These results suggest that Comet assay performed on lymphocytes is a valuable genotoxic marker for high levels of exposure to AFB1 in guinea pig. Additionally our results indicate that the exposure to this toxin increases significantly and increases the level of DNA damage in liver cells, which is a key step on liver cancer development. We also suggest that the Comet assay is an useful tool for monitoring the genotoxicity of AFB1 in liver. © 2007 Springer Science+Business Media B.V.

DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi

This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals); Trypanosoma cruzi infected; T. cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. The results obtained with the single cell gel (comet) assay showed that T. cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly, T. cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (p < 0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the T. cruzi infected group. Taken together, these results indicate that T. cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced T. cruzi genotoxic effects in spleen cells. © 2006 Elsevier Inc. All rights reserved.

Paracoccidioidomycosis: No genetic damage in human peripheral blood cells of patients assessed by single-cell gel (comet) assay

Paracoccidioidomycosis is a systemic fungal infection caused by Paracoccidioides brasiliensis. As infectious diseases can cause DNA damage, the authors aimed at analyzing DNA breakage in peripheral blood cells of patients with paracoccidioidomycosis by using the comet assay. The results suggested that paracoccidioidomycosis does not cause genotoxicity.

Influence of endogenous and synthetic female sex hormones on human blood cells in vitro studied with comet assay

The comet assay has been conducted with numerous cell lines to assess in vitro genotoxicity. In order to use the comet assay as part of an in vitro test for evaluating genotoxicity, however, there are cell-specific factors that need to be better understood. In this present study we have evaluated some factors that may impact upon the DNA damage detected in whole blood (WB) cells and lymphocytes (ILs). Experiments were conducted comparing responses of both cells, and investigating the effects of the female hormonal cycle, and oral contraceptive (OC) use on DNA damage detection in the in vitro comet assay, at three sampling time. No significant differences were detected in the basal levels of DNA damage detected in ILs and WB cells from women OC users and non-users and from men. Basal DNA damage in ILs was unaffected by gender and stage of the menstrual cycle or the stage of the treatment schedule. Our results also indicated that the H2O2 induces DNA damage in human lymphocytes independently of gender, low-dose OC use and hormonal fluctuation. However, data showed that in 3rd sampling of menstrual cycle, lymphocytes were more resistant to H2O2-induced DNA damage than those from OC users and men. © 2007 Elsevier Ltd. All rights reserved.

Z gamma production and limits on anomalous ZZ gamma and Z gamma gamma couplings in pp collisions at root s=1.96 TeV

We present a study of eeγ and μμγ events using 1109 (1009) pb-1 of data in the electron (muon) channel, respectively. These data were collected with the DØ detector at the Fermilab Tevatron p over(p, ̄) collider at sqrt(s) = 1.96   TeV. Having observed 453 (515) candidates in the eeγ (μμγ) final state, we measure the Zγ production cross section for a photon with transverse energy ET > 7   GeV, separation between the photon and leptons Δ Rℓ γ > 0.7, and invariant mass of the di-lepton pair Mℓ ℓ > 30   GeV / c2, to be 4.96 ± 0.30 (stat . + syst .) ± 0.30 (lumi .)   pb, in agreement with the Standard Model prediction of 4.74 ± 0.22   pb. This is the most precise Zγ cross section measurement at a hadron collider. We set limits on anomalous trilinear Zγγ and ZZγ gauge boson couplings of - 0.085 < h30 γ < 0.084, - 0.0053 < h40 γ < 0.0054 and - 0.083 < h30 Z < 0.082, - 0.0053 < h40 Z < 0.0054 at the 95% C.L. for the form-factor scale Λ = 1.2   TeV.

Search for Randall-Sundrum gravitons with 1 fb(-1) of data from p(p)over-bar collisions at root s=1.96 TeV

We search for decays of Kaluza-Klein excitations of the graviton in the Randall-Sundrum model of extra dimensions to e+e- and in 1 fb-1 of pp collisions at s=1.96 TeV collected by the D0 detector at the Fermilab Tevatron. We set 95% confidence level upper limits on the production cross section times branching fraction, which translate into lower limits on the mass of the lightest excitation between 300 and 900 GeV for values of the coupling k/M Pl between 0.01 and 0.1. © 2008 The American Physical Society.

Study of direct CP violation in B±→J/ ψK±(π±) decays

We present a search for direct CP violation in B±→J/ ψK±(π±) decays. The event sample is selected from 2.8fb-1 of pp̄ collisions recorded by D0 experiment in run II of the Fermilab Tevatron Collider. The charge asymmetry ACP(B+→J/ψK+)=+0.0075±0. 0061(stat)±0.0030(syst) is obtained using a sample of approximately 40000 B±→J/ψK± decays. The achieved precision is of the same level as the expected deviation predicted by some extensions of the standard model. We also measured the charge asymmetry ACP(B+→J/ψπ+)=-0. 09±0.08(stat)±0.03(syst). © 2008 The American Physical Society.

Search for scalar neutrino superpartners in e+mu final states in p(p)over-bar collisions at root s=1.96 TeV

We report a search for R-parity-violating production and decay of sneutrino particles in the eμ final state with 1.04±0.06fb-1 of data collected with the D0 detector at the Fermilab Tevatron Collider in 2002-2006. Good agreement between the data and the standard model prediction is observed. With no evidence for new physics, we set limits on the R-parity-violating couplings λ311′ and λ312 as a function of the sneutrino mass. © 2008 The American Physical Society.

First study of the radiation-amplitude zero in W gamma production and limits on anomalous WW gamma couplings at root s=1.96 TeV

We present results from a study of pp̄→Wγ+X events utilizing data corresponding to 0.7fb-1 of integrated luminosity at s=1.96TeV collected by the D0 detector at the Fermilab Tevatron Collider. We set limits on anomalous WWγ couplings at the 95% C.L. The one-dimensional 95% C.L. limits are 0.49<κγ<1.51 and -0.12<λγ<0.13. We make the first study of the charge-signed rapidity difference between the lepton and the photon and find it to be indicative of the standard model radiation-amplitude zero in the Wγ system. © 2008 The American Physical Society.

Observation of the Bc meson in the exclusive decay Bc→J/ψπ

A fully reconstructed Bc→J/ψπ signal is observed with the D0 detector at the Fermilab Tevatron pp̄ collider using 1.3fb-1 of integrated luminosity. The signal consists of 54±12 candidates with a significance that exceeds 5 standard deviations, and confirms earlier observations of this decay. The measured mass of the Bc meson is 6300±14(stat)±5(syst) MeV/c2. © 2008 The American Physical Society.

Search for decay of a fermiophobic higgs boson hf→ y y with the D0 detector at s=1.96a TeV

We report the results of a search for a narrow resonance decaying into two photons in 1.1fb-1 of data collected by the D0 experiment at the Fermilab Tevatron Collider during the period 20022006. We find no evidence for such a resonance and set a lower limit on the mass of a fermiophobic Higgs boson of mhf>100GeV at the 95% C.L. This exclusion limit exceeds those obtained in previous searches at the Fermilab Tevatron and covers a significant region of the parameter space B(hf→I I ) vs mhf which was not accessible at the CERN Large Electron-Positron Collider. © 2008 The American Physical Society.

Measurement of the inclusive jet cross section in pp̄ collisions at s=1.96TeV

We report on a measurement of the inclusive jet cross section in pp̄ collisions at a center-of-mass energy s=1.96TeV using data collected by the D0 experiment at the Fermilab Tevatron Collider corresponding to an integrated luminosity of 0.70fb-1. The data cover jet transverse momenta from 50 to 600 GeV and jet rapidities in the range -2.4 to 2.4. Detailed studies of correlations between systematic uncertainties in transverse momentum and rapidity are presented, and the cross section measurements are found to be in good agreement with next-to-leading order QCD calculations. © 2008 The American Physical Society.

Search for long-lived particles decaying into electron or photon pairs with the D0 detector

In this Letter we report on a search for long-lived particles that decay into final states with two electrons or photons. Such long-lived particles arise in a variety of theoretical models, such as hidden valleys and supersymmetry with gauge-mediated breaking. By precisely reconstructing the direction of the electromagnetic shower we are able to probe much longer lifetimes than previously explored. We see no evidence of the existence of such long-lived particles and interpret this search as a quasi model-independent limit on their production cross section, as well as a limit on a long-lived fourth generation quark. © 2008 The American Physical Society.

Search for neutral higgs bosons in Multi-b-Jet events in pp̄ collisions at s=1.96TeV

Data recorded by the D0 experiment at the Fermilab Tevatron Collider are analyzed to search for neutral Higgs bosons produced in association with b quarks. This production mode can be enhanced in the minimal supersymmetric standard model (MSSM). The search is performed in the three b quark channel using multijet triggered events corresponding to an integrated luminosity of 1fb-1. No statistically significant excess of events with respect to the predicted background is observed and limits are set in the MSSM parameter space. © 2008 The American Physical Society.