Pesquisa do Paracoccidioides brasiliensis em aerossóis ambientais: padronização de metodologia e avaliação de amostras relacionadas ao habitat de tatus positivos

P.brasiliensis é o agente etiológico da Paracoccidioidomicose, micose sistêmica mais importante na América Latina. A fase saprofítica produtora dos propágulos infectantes e outros aspectos ecológicos deste patógeno são ainda pouco conhecidos, o que impede a adoção de medidas preventivas. Diversas evidências indicam que o fungo se desenvolve no solo, porém o seu isolamento a partir destes materiais é raro e apenas eventual. O presente projeto visou desenvolver uma metodologia para obtenção de amostras ambientais na forma de aerossóis em locais onde a ocorrência do fungo já foi evidenciada pelo seu isolamento em tatus Dasypus novemcinctus e avaliar a ocorrência do patógeno nestes materiais por cultura direta e por biologia molecular em reações de PCR com “primers” específicos, já desenvolvidos em nosso laboratório. Para tal, avaliamos o padrão de crescimento do P. brasiliensis em quatro diferentes meios ditos como seletivos. Entre estes meios, encontra-se o Meio Sólido de amônia que, por nunca ter sido testado em P. brasiliensis, necessitava de testes quanto à viabilidade de uso e ajuste de concentração. Depois de testado a funcionalidade dos meios e observado o padrão de crescimento que o fungo exibe nesses diferentes meios, partimos para a tentativa de isolamento ambiental do P. brasiliensis através de amostras de solo em forma de aerossóis com o auxílio deles. Tendo em vista os resultados obtidos, optamos, também, por realizar a coleta de teias de aranhas, devido a grande aderência que exerce sobre aerossóis ambientais. Os isolados de P. brasiliensis cultivados apresentaram desenvolvimento semelhante nos quatro meios utilizados, indicando a possibilidade de uso destes meios seletivos para estudos ambientais. P.brasiliensis não foi isolado nestas amostras ambientais... (Resumo completo, clicar acesso eletrônico abaixo)

Análise de métodos de mapeamento de perigo: uma proposta de elaboração de mapeamento de perigo de escorregamento planar de solo via utilização do método participativo

This final assignment aimed to promote a critical analysis of methods of preparation mappings danger of landslide and presentation of proposed development from the use of participative method, as an instrument to facilitate the identification of adverse geological processes. There search was executed from literature review about concept associated with geological dangers theme and the typology and characteristics of gravitational mass movements. We analyzed particularly the so-called methods of mapping danger tradicional/classic and alternative. It is believed, that integrating the work of specialists with the participation of involvedagents (local community and stake holders). This literature review and proposed work do noted in itself. You need the continuing advance in the discussion, debates and research on this topic as well as public policies that act on problems identified more concretely

Avaliação da produtividade em áreas de campo sujo com diferentes históricos de fogo

Fire is a common event in different ecosystems and can both be caused by humans or have natural sources.. In many of these ecosystems, natural fires are an important factor that determines the vegetation. The reduction of tree cover by fire for example, resulted in the evolution of several species-rich ecosystems, dominated by C4 grasses. However, the fire caused by human actions may have greater intensity and lead to negative responses of vegetation, since man changed the fire regime in many parts of the world, such as in the Cerrado. The passage of fire can benefit herbaceous and woody seedlings that cannot compete with the dominant grass layer. It removes the dead biomass and litter (major components of the fuel load), opening up spaces within the grass matrix that allow the establishment of other species. After some time without fire, an increase in shrub cover and decrease herbaceous layer can be observed. One of the major consequences of the absence of fire in savanna and grassland ecosystems is the accumulation of flammable dead biomass (mainly composed of graminoids), which will probably be the fuel load of the next burning thus, fires will be more intense and hotter. Moreover, very frequent burns lead to a reduction in the frequency and density of grasses. Therefore, this study aimed to assess the quantity and quality of biomass in areas with different fire history (fire exclusion for 2 and 7 years) in areas of campo sujo in central Cerrado. Plots (1x1m) were established in both areas and all aboveground biomass of each plot was cut at ground level and put in paper bags in the field. In the laboratory, the material was sorted into live and dead biomass. In addition, live biomass was separated into different functional groups (graminoids, forbs, Vellozia spp, palm and shrubs). The material was oven dried for two days at 80°C and subsequently weighed. In both areas, we found a dominance of graminoid and dead biomass. The area...

Avaliação da toxicidade da vinhaça utilizando Oreochromis niloticus (Perciformes: Cichlidae) como organismo teste

Contamination of rivers and lakes, waste disposed by infiltration into the soil as fertilizer, for example, has been highlighted around the world, since such compounds can contain toxic elements, causing changes in aquatic and/or terrestrial ecosystem. Due to the possible presence of these elements in these wastes, it is possible assess damage to organisms exposed through breaks in genetic material and mutations. In this sense, this work aimed at exploring the cytotoxic, mutagenic and genotoxic vinasse pontential, residue used as fertilizer, from the processing of sugar cane into alcohol, using fish (Oreochromis niloticus - Cichlidae) as a test organism. The individuals were exposed to different dilutions of vinasse in the water and later carried out analysis of their erythrocytes using the micronucleus test and nuclear abnormalities associated and the comet assay. In the first bioassay, tilapia exhibited 5% and 10% vinasse concentrations died after 48h. The number of micronucleated erythrocytes was statistically significant for the 1% vinasse concentration and also for nuclear abnormalities such as broken-egg and nuclear bud, when compared with negative and positive controls; were also significant lobed type erythrocytes, when compared to the negative control. The test results of the comet assay were not significant for 1% vinasse when compared to the negative control, due to the high rate of comets with damage control, which is unusual. The same was observed for changes as notched and blebbed type. In the second chemical analyzes conducted, the detection of heavy metals in the vinasse sample, charged only the presence of copper and chromium, the latter with a concentration 89 times higher than in the first test. Differentially the first bioassay, fish exposed to dilution of 5% did not die... (Complete abstract click electronic access below)

Avaliação da erosão hídrica, transporte de sedimentos e qualidade da água na bacia do córrego Monjolo Grande

Understanding the linkages between the natural elements is essential for being promoted the land use, occupation and sustainable management of environmental systems. Universal Soil Loss Equation (USLE), as a predictive model of erosion, is important to allowing the prevention of possible environmental impacts which may drastically interfere in natural or anthropic environments, as well as prevent potential financial wastes and even contribute to greater efficiency of agricultural production. This research will be working some USLE parameters, emphasizing topographic factor from Ribeirão Monjolo Grande watershed. Among the factors considered by the USLE, the Topographic Factor interferes directly in the erosive dynamic of a watershed because it involves variables related to hydrological processes that occur on it. In this research, were discussed different methods for obtaining the Topographic Factor (BERTONI e LOMBARDI NETO, 1985; MOORE e BURCH, 1986; DESMET E GOVERS, 1996) in GIS environment. After comparison between the methods, was indicated that best represents the conditions of geometry strand of the study area. Finally, other factors (R, K, C, P) considered by the USLE were obtained. The attainment of these parameters were guided by the use of geotechnologies, especially in Geographic Information Systems (GIS), with the assistance of secondary data and periodic field visits. The results obtained contributed to the understanding of hydrosedimentological dynamic in this area and serve as a viable strategy for studies of soil loss, aiming at developing consistent material for future researches about environmental planning and land management

Avaliação do comportamento da temperatura na massa deresíduos com diferentes tempos de disposição no aterro sanitário de Rio Claro - SP

The present work investigates solid waste temperature behavior in different depths in two cells in the Rio Claro-SP city’s sanitary landfill. One of the cells is in operation with waste disposal beginning about one year ago. The other one is located in an closed area and contain waste disposed from five to seven years before. Measures were also made in an area that have no disposed waste in order to collect reference values. The data were obtained every fifteen days. The temperature results shows higher values in the operating cell, with maximum 38,1 °C in a depth of 4,0 m. In the closed cell the highest values were 36,3 °C in a depth of 8,5 m. The highest temperature values were obtained in the operating cell due to wider substract availability that indicates a more intense biological degradation activity. With three meters depth, the temperature results were 36,6 °C in the operating cell, 33,8 °C in the closed cell and 24,5 °C in the reference area. Therefore the temperature can be used as a biological activity indicator in sanitary landfills, supporting biogas studies

Avaliação da toxicidade do cloreto de níquel utilizando Allium cepa como organismo teste

O impacto das atividades antropogênicas sobre o meio ambiente é um tema de grande relevância atualmente, atraindo a atenção da comunidade científica em geral, visto que essas atividades têm causado alterações significativas na qualidade da água, do solo e do ar. Dentre a ampla gama de poluentes resultantes das atividades humanas, merece destaque a classe dos metais pesados, os quais possuem propriedades tóxicas e genotóxicas, constituindo fatores de risco para o desenvolvimento de desordens neurodegenerativas, artrite e câncer nas populações expostas a estes contaminantes, bem como alterações nos ecossistemas contaminados. Neste sentido, o presente trabalho teve por objetivo avaliar o potencial citotóxico, genotóxico e mutagênico do níquel como contaminante, utilizando Allium cepa (Liliaceae) como organismo-teste, a fim de detectar possíveis efeitos deletérios em seu material genético. Estas avaliações foram feitas por meio da exposição de sementes de A. cepa a três soluções, com diferentes concentrações de níquel. Os resultados obtidos no primeiro biensaio e na repetição estão apresentados na forma de artigo científico, a ser submetido para revista especializada. O estudo dos efeitos do níquel em A. cepa foram importantes, uma vez que os dados da avaliação dos danos induzidos neste organismo modelo podem ser utilizados para a comparação com modelos animais, sendo possível assim, a inferência dos perigos aos ecossistemas e à população que está exposta a esse contaminante

Uso do modelo de simulação 'ABC 6' para avaliação de áreas sujeitas à ocorrência de ondas de cheia e corridas de massa/detritos: estudo de caso na bacia do Ribeirão da Prata (SP)

Pipelines are linear engineering works, designed mostly for transporting oil and its derivatives for long distances, furnishing even the farthermost zones of the country. Due to oil sector needs to ensure for the safety and conservation of its properties, several geotechnical studies are being held at the pipelines field, in order to preserve this important transportation, and also to prevent accidents, which might seriously compromise the environment and the population who lives around it. The OSBRA pipeline, who connects the city of Paulínia to the capital Brasília, is one of these engineering works that deserves to be pointed out. This research, performed at the Ribeirão da Prata Basin, was a pilot study conducted with the main objective of testing the current methodology efficiency, for future applications in the closest watersheds to the OSBRA pipeline. The objective of this research is to analyze flood wave and debris flow processes in a non-fictional watershed, by comparing two different kinds of methods: the first one based on simulation models (software ‘ABC 6’), and the other one by flood wave and debris flow susceptibility mapping. The results from the hydrological modeling were both hydrographs and ietographs that estimated values of outputs and infiltration. To construct the susceptibility maps were necessary three other maps: ground use and occupation maps, divided according to the different protection degrees that were offered to the ground; maps of dam locations in the area and physiographic compartimentation maps, divided according to the local geology. To complete the methodology, the results were collected from both methods for comparison. The obtained product for this methodology was series of data whose different susceptibility degrees to flood wave and debris flow could define the safest route for a pipeline crossing in this watershed...

Avaliação ambiental em empresas do distrito industrial Antônio Crepaldi, Presidente Prudente/SP

The sustainable development is the center of many discussions about the environment, economy and society. Environmental issues bring out problems with the fauna and flora, water resources, land use, population growth, residues, basic sanitation, and others. In this sense, the aim of the present work was to develop an environmental diagnostics and propose measures to mitigate the most significant environmental impacts generated by four enterprises in Antonio Crepaldi Industrial District, located at the city of Presidente Prudente/SP. In the study, it was used the methodology proposed by Moreira (2006), and it was done the following activities: monitoring and analyses of the production process, identification of potential environmental aspects and impacts; assessment of the impacts according to its nature, relevance and significance; proposition of measures to mitigate the critical impacts. As a final result, it was obtained an environmental diagnosis about the situation of each of the four companies studied, and it was possible to know the level of criticality of the impacts in each one, and proposing mitigation measures to control or minimizing them, bringing many benefits to the organization

Avaliação do efeito tóxico do herbicida trifluralina por meio do estudo histológico e histoquímico do intestino médio do diplópodo Rhinocricus padbergi

Currently the use of pesticides in agriculture is widespread due to their high effectiveness in combating pests, weeds and diseases leading to better productivity and economical performance in agricultural area. The use of pesticides affects the whole world and their use is often performed in an improper and indiscriminate way and for long periods. Several studies have been carried out in order to verify the presence of pesticides in nature, with worrying results. The presence of higher levels of pesticides and their degradation products in soil and surface and groundwater have indicated increasing contamination. Among the most widely used pesticides, herbicides are present and among these trifluralin has occupied an important place due to its widespread use; it is an herbicide originated from benzene derivatives belonging to dinitroanilines family; it is classified as belonging to group C, being possibly carcinogenic for humans, present a high persistence in soil as a result of its low mobility and therefore may affect local edaphic fauna. Diplopods belong to a group of invertebrates considered important in the soil dynamics; due to their close contact with it, these animals can be used as bioindicators of substrates toxicity. This study aimed to expose diplopod specimens of the species R. padbergi to different concentrations of trifluralin and therefore it was mounted five bioassays containing soil from the site where animals were collected (control group) and the same soil mixed to different concentrations of trifluralin herbicide (concentration recommended for agriculture use, that is, the dose recommended by the producer 0.0534g/m2, double, haft and quarter of this dose), animals were exposed for 7 and 90 days. During the entire period of exposure (90 days), it was observed that the number of animals in the control bioassay remained stable until the 5th week, presenting only... (Complete abstract click electronic access below)

Avaliação das taxas de erosão química e física na bacia hidrográfica do Ribeirão Jacutinga (SP)

Currently with the growing technological advancement and global, new areas are being devastated, and as a result, the erosion became more frequent causing loss of soil, infiltration capacity of the surface and weathering of rocks. In this way, environmental studies are increasingly frequent to understand the environment in which we live, but also to avoid any degradation of the same and of finite natural resources, which are essential for human life. From this, this work has as main goal, analyzing the rates of chemical and mechanical erosive watershed of Ribeirão Jacutinga, at its mouth, in the city of Rio Claro, supporting environmental studies and sustainable development today. Order to reach these values were measured and total dissolved solids in suspension in the stream during the months from January to July 2012. As results included higher rates of both physical and chemical weathering in the rainy months, especially january

Avaliação quali-quantitativa das parcelas escoadas, percoladas e armazenadas em instalação experimental piloto de telhado verde extensivo com diferentes inclinações

This study investigated the effect of slope and antecedent soil moisture on the water depth stored and percolated on extensive green roofs built in pilot scale. For this purpose, slopes of 10, 20 and 30% were investigated. Moisture was measured before and after each test to determine the differential moisture (ΔU). A rainfall simulator was designed and tested; the best arrangement presents a Christiansen Uniformity Coefficient of 56%. The results yielded that the slopes of 10% presented the highest values of water depth stored (59,88%), water depth percolated (77,15%), the slope of 30% presented the highest water depth drained (48,22%.) and there was no correlation between slope, water depth stored, water depth percolated and ΔU. It was verified the effect of dilution on the modules i10% between water depth percolated and apparent color (0.71%) in i20% between water depth drained and apparent color (-0.748%) and in the module i30% between water depth drained and turbidity (-0.76%) and water depth drained and apparent color (-0.93%). In i10% there was high correlation between pH and water depth percolated (0.94%) and between water depth drained and pH (0.71%). The data indicate that the module i10% had a greater reduction of runoff

Avaliação da influência do enriquecimento ambiental nas variáveis comportamentais de saguis-de-cara-branca (Callithrix geoffroyi) em cativeiro

Six species of the genus Callithrix can be found across the Atlantic Forest extension, being the Callithrix geoffroyi, Geoffroy`s marmoset, the least studied species. The objective of this study is to investigate the behavioral aspects of the species in captivity, and how the environmental enrichment techniques influence those behaviors. To this end, we have studied three specimens of C. geoffroyi, in captivity at Municipal Zoo of Piracicaba, São Paulo. The enclosure includes a 37m2 with under masonry, concrete platforms with external deep, trunks, a floor of grass and soil, e grid in their other three sides. The sampling method was focal per time interval, with records every 30 seconds in sessions of 30 minutes, totaling 30 hours for each animal, which were distributed in a baseline phase, an environmental enrichment phase and post-enrichment phase (n = 3660 records in each phase). The twelve enrichment techniques were applied: the physical environment (branches for perches and hoses fabrics for bedding and vanishing points), cognitive (radio, mirror, puzzle food) and food (insects, flowers and eggs hidden, wrapped). Initially, to determine the period of greatest activity of marmosets, we investigated the pattern of activity for three days (9:00 am to 17:00 pm) consecutively at intervals of 15 minutes, recording the number of active and inactive individuals. The 495 records signaled greater activity in the morning, then selected period for the completion of this work. The ethogram comprised 16 behaviors. The inactivity was the predominant phase behavior baseline and post-enrichment with a significant decrease (h=8,62, p <0.01) in the enrichment phase, and a significant increase in the post enrichment (h=18,15, p <0.001). The same was seen for the use of substrate (grid and ground vs. Trunk and concrete, h=5,09, p <0.001 and h=3,98, p <0.05)... (Complete abstract click electronic access below)

Avaliação da degradação da mata ciliar no entorno do rio Corumbataí causada pela expansão urbana de Rio Claro/SP

In most brazilian cities, urban growth has occurred without adequate planning. Consequently, portions of the city were being occupied, often areas unfit for use and occupation of land, causing risks to local residents and decline of urban environmental quality. One of the most frequent problems is the riverbank occupation, causing the deforestation of the riparian vegetation. Given the above, this paper aimed to map the urban sprawl of Rio Claro/SP, as well as riparian vegetation in the vicinity of Corumbataí in the area including the urban area. For this purpose, GIS techniques were used in order to prepare the thematic map of urban expansion, as well as a map of the riparian vegetation through interpretation of aerial photographs in the environment of ARCGIS. From the maps, an analysis of “Plano Diretor” was made in order to verify if the current occupation of urban land meets the stipulations of this legal instrument

A antropogeomorfologia na bacia do Córrego do Cavalheiro - Analândia/SP: uma avaliação da dinâmica do uso da terra e sua adequabilidade a legislação ambiental e a capacidade de uso

The assessment of land use dynamic can be an instrument for analysis in anthropogeomorphology, which would allow one to verify the human geomorphological actions and its various implications in changing the original morphology for the creation of a human morphology. This study aimed to consider the land use dynamics and its interference in the morphology of Cavalheiro Stream Basin. From this assessment, to understand the implications of land use in the creation of a human morphology, as well as to analyze if the current use is in accordance with the legal restrictions placed by ongoing environmental legislation and with the ability to land use. The chosen of this studied area was due to assumptions raised by Pinton (2007) for changes in the dynamic of rainwater erosion of this area caused by human actions, specially the cultivation of sugar cane in morphologies that are unfit for it. The search took as methodological support the principles that concern the General Systems Theory, trying to see the watershed as an open system. The geomorphological data, of land use and of legal restrictions to the use and occupation of land, were collected from different cartographic techniques. Besides, it was developed a sketch map of land use capacity by adapting the technique proposed by Lepsch (1983). This way, the data obtained in this survey are considered capable to provide subsidies for the identification of changes in original morphology of the Cavalheiro Stream Basin and the genesis of a human morphology, as well as conflicts and adjustments of current land use in environmental system and on the legal restrictions outlined in the ongoing environmental legislation, contributing to a proper environmental planning with the characteristics of the physical environmental system of this basin