Reatividade e tolerância ao calor de fêmeas ovinas de diferentes grupos genéticos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Características de dossel e produção de milho submetido a arranjos espaciais de planta e adubação nitrogenada em plantio direto

Pós-graduação em Agronomia (Ciência do Solo) - FCAV

Estudo da distribuição de tamanho e composição iônica de aerossóis e seus efeitos na capacidade de nuclear gotas de nuvens

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Caracterização dos compostos derivados de furoxano como inibidores da atividade de bombas de efluxo em Mycobacterium tuberculosis e estudo da influência do efluxo e da halotolerância nos perfis de resistência do bacilo

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

P-220-Escherichia coli displaying virulence features of multiple diarrheogenic pathotypes detected in a Crohn's disease patient

Background: The number of Escherichia coli in the gut of Crohn's disease (CD) patients is higher than that of normal subjects, but the virulence potential of these bacteria is not fully known. Previous studies have shown that these E. coli are closely related to extraintestinal pathogenic categories (ExPEC), are able to invade epithelial cells, and usually do not produce exotoxins. We report here the detection, in a CD patient, of an E. coli which belongs to a classical enteropathogenic (EPEC) serotype and displays virulence markers of enteroinvasive (EIEC), enteroaggregative (EAEC) and enterohemorrhagic (EHEC) pathotypes. Methods: The E. coli strain was isolated, in 2009, by classical bacteriological procedures from a 56 year old woman who underwent ileo-terminal resection 1 year before, due to intestinal obstruction. The bacterial characterization was carried out by in vitro adhesion and invasion assays to cultured epithelial cells and macrophages and screening by PCR to identify virulence genetic markers of diarrheogenic E. coli (DEC) and to detect one of the gene combinations which define the phylogroups of the E. coli reference (EcoR) collection. The strain was also tested for the ability to produce biofilm and shiga cytotoxins and had its whole genome sequenced by Ion Torrent Sequencing Technology. Results: The studied strain, which was detected both in ileum biopsies and the stools of the patient, displayed the aggregative adherence (AA) phenotype to Hep-2 cells and an ability to enter Caco-2 cells 3x as high as that of EIEC reference strain and 89% of that of the prototype AIEC LF82 strain. Although it could invade cultured macrophages, the strain was unable to replicate inside these cells. PCR screening revealed the presence of eae, aggR and stx1. Tests with bacterial culture supernatants in Vero cells demonstrating cytotoxicity suggested the production of Stx1. In addition, the strain revealed to be a strong biofilm producer, belonged to the B2 EcoR phylogroup, to the O126:H27 serogroup and to the multilocus sequencing type (MLST) ST3057. The 2 later features were deduced from the whole genome sequence of the strain. Conclusions: The characterization of this E. coli isolate from a CD patient revealed a combination of virulence markers of distinct DEC pathotypes, namely eae and stx1 of EHEC, AA, aggR and biofilm formation of EAEC, and invasiveness of EIEC. These features along with its serotype and phylogroup identity seem to suggest a potential to be involved in CD, an observation which should be tested with additional studies.

Valor nutritivo do capim Piatã adubado com diferentes doses de biofertilizante

The aim of this study was to verify the influence of different doses of biofertilizers made from waste produced by cattle and pigs at growing-finishing on nutritive value of Piatã grass. The experiment was carried out at green house using a split plot design with a completely randomized factorial. Plots were divided into eight treatments: four doses of two biofertilizers, and four different periods of subplots cut. Biofertilizers were applied at a single dose, after the cut to standardize the plots, using doses of Nitrogen of 0, 100, 200 and 300 kg ha-1 . The results showed no significant difference among types of biofertilizer and also, the biofertilizer x dose interaction was not significant, making possible to recommend both of biofertilizers, with no risk of plant injure. Contents of neutral detergent fiber, acid detergent fiber, cellulose, hemicellulose and lignin showed negative linear behavior as a function of increasing doses of nitrogen. Contents of crude protein and “in vitro” dry matter digestibility coefficients replied linearly and positively to nitrogen doses. It also observed the effect of cut periods, since plants that were cut in summer showed nutritive value superior to those plants that were cut in autumn.

Limnology of a lateral lagoon system connected to a Neotropical reservoir (Rosana reservoir, São Paulo/Paraná, Brazil)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desempenho agronômico e diagnose foliar da aveia preta em função da calagem em plantio direto

The soil acidity in no tillage system could be resolved by lime superficial application, improvement to crop mineral nutrition and yield. The experiment carried out on a Rhodic Kandiudalf in Botucatu, São Paulo State, Brazil, in dry conditions, aiming to evaluate the agronomic development and leaf diagnosis of black oat plants, under superficial liming in no tillage system. A randomized complete block design was used, with four replications, the treatments were superficial application of different dolomítico lime rates (R0 = zero – without lime; R1 = 1,8 t ha-1 – lime to increase the base saturation at 50%; R2 = 3,6 t ha-1 – lime to increase the base saturation at 70% and R3 = 5,4 t ha-1 – lime to increase the base saturation at 90%), in October 2002. The black oat Common cultivar was seed in April 2004, in to second year, after the crop rotation of millet (spring) – common bean (summer) – black oat (autumn-winter). The results showed that: the black oat had yield increase by superficial liming, mainly to most dry matter and grain per panicule. Beyond, the superficial liming application did not prejudice leaf diagnosis of culture.

Comparação de custo do milho fertilizado com adubo industrial e com lodo de esgoto a custo zero

In this study, we compared the production cost of summer corn growing in soils treated with industrial fertilizer with those grown treated with sewage sludge. Corn was cultivated on reduced tillage treatment during 2002/2003 in an Oxisol area of 0.0625 hec. The field trial with industrial fertilizer was conducted following related guidelines for the state of Sao Paulo. The application of sewage sludge to the soil was at the rate of 10 t.ha-1. Calculations were based on market prices for the abovementioned years. In addition to lower environmental impact, results indicate economic cost advantages associated with the use of sewage sludge.

Estimativas da radiação difusa horária incidente em superfícies inclinadas com base na transmissividade atmosférica

Evaluated statistical equations estimates (based on radiometric fractions) of the hourly diffuse radiation incident on inclined surfaces for the North to 12.85, 22.85 and 32.85°, the climate and geographical conditions of Botucatu, SP. The database was generated from April/1998 to December/2007, with measures in the three tilted surfaces in different periods, but concomitant to the horizontal plane. In the validation of the equations were used indicative statistics MBE (mean absolute error), RMSE (square root mean square error) and index adjustment (d) for three inclinations and conditions of sky coverage. The increased angle of inclination of the surface led to increased scattering of hourly values for the coefficient of atmospheric transmissivity of diffuse radiation for inclined and horizontal surfaces. Estimates of diffuse radiation on the basis of hourly tilted horizontal global radiation occur for quadratic polynomial models, which adjust K'Dβ maximum values of between 0.14 and 0.30 for winter and summer when KTH varies between 0.40 and 0.66, indicating that energy, the highest values of diffuse radiation occur in partly cloudy sky conditions and / or partially open. The increase in atmospheric transmissivity decreases the performance of annual and monthly equations at all inclinations.

Desempenho agronômico e produtivo de cultivares de soja em diferentes ciclos e safras

With the objective of gathering technical data about soybean cultivars performance in Botucatu, state of São Paulo, Brazil, an experiment was conducted to evaluate seventeen genotypes. The experiment was conducted during the Summer seasons of 2002/03, 2003/04, and 2004/05. The experiment was set in the field according to a complete block design with four repetitions. The soybean cultivars were ‘Embrapa 48’, ‘BRS 132’. ‘BRS 183’, ‘BRS 212’, ‘IAC 22’, and ‘IAC 23’ (early cycled varieties), ‘BRS 133’, ‘BRS 154’, ‘BRS 156’, ‘BRS 184’, ‘BRS 214’, ‘IAC 18’, and ‘IAC 24’ ( semi early varieties), and ‘BRS 134’, ‘BRS 215’, ‘IAC 8.2’, and ‘IAC 19’ (medium cycled varieties ). All the varieties, during the three cropping years, showed adequate plant height and first pod height of insertion for mechanical harvest. Among the production components, mass of 100 grains showed the highest variability. Cultivar ‘BRS 154’ (medium cycle) showed the highest variation in mass of 100 grains and was also the highest yielding variety in the cropping year of 2004/05. The majority of the cultivars yielded above 3,000 kg ha -1 during the cropping years of 2002/03 and 2004/05. The best yielding performance during the three cropping years were displayed by cultivars ‘IAC 22’ (early cycle), ‘BRS 133’ and ‘BRS 156’ ( both semi early cycled varieties).

Discriminação de classes de cobertura vegetal utilizando técnicas de classificação digital de imagens de sensoriamento remoto

The aim of this work is to discriminate vegetation classes throught remote sensing images from the satellite CBERS-2, related to winter and summer seasons in the Campos Gerais region Paraná State, Brazil. The vegetation cover of the region presents different kinds of vegetations: summer and winter cultures, reforestation areas, natural areas and pasture. Supervised classification techniques like Maximum Likelihood Classifier (MLC) and Decision Tree were evaluated, considering a set of attributes from images, composed by bands of the CCD sensor (1, 2, 3, 4), vegetation indices (CTVI, DVI, GEMI, NDVI, SR, SAVI, TVI), mixture models (soil, shadow, vegetation) and the two first main components. The evaluation of the classifications accuracy was made using the classification error matrix and the kappa coefficient. It was defined a high discriminatory level during the classes definition, in order to allow separation of different kinds of winter and summer crops. The classification accuracy by decision tree was 94.5% and the kappa coefficient was 0.9389 for the scene 157/128. For the scene 158/127, the values were 88% and 0.8667, respectively. The classification accuracy by MLC was 84.86% and the kappa coefficient was 0.8099 for the scene 157/128. For the scene 158/127, the values were 77.90% and 0.7476, respectively. The results showed a better performance of the Decision Tree classifier than MLC, especially to the classes related to cultivated crops, indicating the use of the Decision Tree classifier to the vegetation cover mapping including different kinds of crops.

Emissão de gases de efeito estufa e estoque de carbono no solo em função do manejo e correção de acidez

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise de oportunidades de poligeração em edificações e cidades

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Consórcio de milho e Urochloa ruziziensis e inoculação com Azospirillum brasilense e seu efeito residual associado à adubação nitrogenada em feijoeiro de inverno

Pós-graduação em Agronomia - FEIS