565 resultados para digestibilidade de proteína
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Pós-graduação em Bases Gerais da Cirurgia - FMB
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Pós-graduação em Aquicultura - FCAV
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504 (five hundred four) 1 day old male chicks from a commercial broiler line (Ag Ross 308) were used, distributed in 12 treatments, with 6 replicates per treatment. The experimental design was casually blocked and treatments were organized in a 2x2x3 factorial arrangement: two available phosphorus levels (0.45 and 0.34%), two phytase inclusion levels (0 and 1200 FTU/kg) and three crude protein levels (22.5; 20.5 and 18.5%). Tibia calcium percentage was influenced by protein and phosphorus levels in the diet, when using phytase. Tibia ash levels showed a linear effect when enzymes were added and showed a quadractic effect without it, and the higher value was observed with 22.5% crude protein in the diet. With higher phosphorus levels a linear effect was observed between crude protein in the diet and tibia ash weights, as a higher protein level had lower tíbia ash weight.
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The experiment was conducted to evaluate the ingestive behavior of feedlot lambs fed with an exclusive diet of concentrate with different percentages of protein (14, 16, 18, and 20%). A total of twenty four Santa Inês crossbred lambs, not castrated, with approximately 180 days of age, average live weight of 25kg, confined, in a completely randomized design, with four treatments and six repetitions. The time spent on feeding decreased linearly in 0.20 hours/day for each 1% increasing in protein percentage in the diet, the idleness increased linearly in 0.25 hours/day, and the total chewing decreased linearly in 0.25 hours/day. The increase in the protein percentage in the diet increased the feed efficiency linearly (kg DM and NDF/hour) in 0.038 and 0.005kg/hour, respectively, for DM and NDF fractions. However, the intake of dry matter (DM) and neutral detergent fiber (NDF), the rumination efficiency (kg DM and NDF/hour), as well as the period (nº /day) and the time (min) spent on feeding, ruminating and idling were not influenced by different protein percentages in the diet.
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Objetivou-se com este estudo avaliar as perdas fermentativas, composição bromatológica e digestibilidade in vitro da matéria orgânica de silagens de milho produzidas em diferentes estádios de maturidade, inoculadas microbiologicamente. Aplicaram-se dois inoculantes comerciais nas silagens produzidas nos estádios SLL, 1/3 LL, 1/2 LL, 2/3 LL e CN, permanecendo ainda um tratamento sem inoculação (silagem controle), configurando um esquema fatorial 3x5. A inoculação com BAL resultou em menores perdas fermentativas (P=0,0348), ao passo que silagens produzidas com plantas mais secas também apresentam menores perdas de MS (P<0,01). A inoculação das silagens resultou em maiores concentrações de PB nas silagens produzidas nos estádios SLL, 2/3 LL e CN (P=0,0033). O uso do inoculante Maize All® resultou em menor concentração de FDN (P=0,0140) no estádio CN e acréscimo dos coeficientes de DIVMO quando as plantas foram colhidas com 2/3 LL e CN (P=0,0006). As perdas fermentativas diminuem devido à utilização dos inoculantes bacterianos e também em silagens produzidas com plantas mais secas. A aplicação de bactérias ácido-láticas (inoculante Maize All®) em silagens de milho produzidas com plantas em estádio de maturidade mais avançado melhora a composição química e digestibilidade in vitro.
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O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por Western blot. Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Pós-graduação em Zootecnia - FCAV
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Pós-graduação em Zootecnia - FCAV
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Pós-graduação em Biotecnologia - IQ
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This study was carried out in Fazenda Canchim in Sao Carlos-SP, physical base of Embrapa Southeast Livestock, aiming to select, based on the agronomic characteristics of productivity of dry matter and quality of the forage, the accesses of suitable gender Paspalum for possessing potentiality of use as foraging plants. Were evaluated for 3 years, with 22 forage grasses, being 19 of Paspalum and three more of control species: Brachiaria decumbens, Andropogon gayanus cv. Baet and Panicum maximum cv. Tanzania, submitted to two intensification levels: high (fertilizer after each cut and supplemental irrigation) and low (only fertilizer of annual replacement without irrigation). The experimental design was of split blocks subdivided in the space and time with two replications. The studied variables were: dry matter production, in vitro dry matter digestibility and crude protein content. The results show that the accesses responded to the intensification level minimizing the negative effect of the stational production. Based on the studied variables, the selected accesses were: BRA-011401 (Paspalum glaucescens); BRA-011681 (P. atratum); BRA009661 (P. atratum) and BRA-019186 (P. regnellii).
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Pós-graduação em Zootecnia - FCAV
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Pós-graduação em Patologia - FMB
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Pós-graduação em Zootecnia - FCAV
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The oral cancer model in hamsters shows many simílarities with developmental oral cancer in humans. The proliferating capacity is one the most characteristics of neoplásica ce/Is and detection of these ce/Is allow us, throughout of its counting, to achieve an estimated tumour growing index, with a consequent repercussion about prognostic and in the treatment of those lesions. 40 golden Syrian hamsters, both genders, aged between two to six mouth and weight 150g in average were used. The left síde of tangue of each animal was painted for eight consecutive weeks, with a solution of DMBA. Tongues were removed, fixed in 10% buffered fonnal solution. The histological slides were stained by AgNOR technique and by PCNA and β-catenin immunohistochemical antibodies. Statistical analyzes were performed by ANOVA one-way test and Tukey test. We may conclude that an association between AgNOR and PCNA might indicate the higher proliferating activity of the analyzed celIs. The experimental carcinogenesis model in hamster tongue is an available methodology for immunohistochemistry study. And finally, PCNA and β-catenin immunohistochemical antibodies may be used to analyze possible premalignant areas in oral leukoplakia