Molecular characterization of accessions of crabgrass (Digitaria nuda) and response to ametryn

Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psbA gene and evaluate the accessions response to ametryn. As showed by molecular analysis there was high genetic similarity among the accessions, all of them presented similar genetics profiles and were susceptible to ametryn.

Estudo do efeito da composição e da nanoestrutura de nanopartículas de 'PT''NI' suportadas em carbono na eletrocatálise da reação de redução do oxigênio

Pós-graduação em Química - IQ

Use of EP(Peroxidase) allele in soybean varietal characterization

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Marcadores de superfície de Células-tronco: estudo de reação cruzada inter-espécies

Mesenchymal stem cells (MSCs) are adult multipotent cells with fibroblastoid morphology and adherent to plastic. Furthermore, they can be obtained from different sources. Besides bone marrow, these cells are taken from umbilical cord blood, umbilical vein, saphenous vein, peripheral blood, arteries, liver and fetal pancreas, placenta, dental pulp and adipose tissue. MSCs derived from adipose tissue are important because of the abundant number of cells that can be obtained from this tissue, easy access and little discomfort to the patient. This study compared two techniques for obtaining MSCs from adipose tissue: mechanical dissociation (MD) and enzymatic digestion (ED). We also analyzed the inter-species cross-reactions using commercial monoclonal antibodies directed against surface antigens of stem cells from different species: mouse, horse, rabbit, monkey and human. We found that MD technique is favorable in relation to ED within 15 days of culture, and ED is more efficient in the first days of culture. The data also showed that MD causes less damage to cellular DNA. About inter-species cross-reactions, the monoclonal antibody A69 directed against stem cells from rabbits, which can be used in veterinary medicine, particularly in research involving horses

Estudo comparativo do uso da terapia fotodinâmica antimicrobiana (aPDT) com uso sistêmico de amoxicilina e metronidazol, no tratamento da periodontite crônica em pacientes fumantes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Quantificação de enterobactérias e Clostridium spp. e detecção molecular de Clostridium perfringens, Escherichia coli e Salmonella spp. em pontos de cadeia produtiva da carne de frango

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação histológica e videolaparoscópica de ligaduras dos pedículos ovarianos realizados com mononáilon agulhado ou abraçadeiras auto-estáticas de náilon em cadelas submetidas à ovariossalpingohisterectomia pela técnica do gancho

A procriação excessiva de cães e gatos no ambiente urbano é um problema de saúde pública e de bem estar animal. Para se desenvolver campanhas de controle populacional necessita-se de protocolos cirúrgicos seguros, rápidos e de baixo custo. A abraçadeira auto-estática de náilon aparentemente se encaixa neste perfil. Objetivou-se avaliar por laparoscopia e histologia, a reação tecidual após a ligadura dos pedículos ovarianos com abraçadeira auto-estática ou mononáilon agulhado na ovariosalpingohisterectomia em 18 cadelas. Após 60 dias do procedimento cirúrgico não foi observada pela laparoscopia nenhuma aderência ou alteração macroscópica e a histologia dos fragmentos dos pedículos ovarianos demonstrou que a resposta tecidual não diferiu entre os métodos, revelando que a abraçadeira auto-estática pode ser usada como método de ligadura dos pedículos ovarianos de cadelas submetidas à ovariosalpingohisterectomia.

Utilização de material alternativo para a obtenção e caracterização de biomodelos, por meio da técnica de impressão 3DPRINTER

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da cadeia petrolífera no Brasil: uma contextualização das tendências da indústria nacional e das relevâncias do PRH48

In the past few years, the Brazilian oil and gas sector had a considered growth, gaining enough relevance at international level, and being extremely important for the Brazilian socioeconomic development. However, the sector is still limited due to technical deficiencies. This work aims to conduct a current study, comprehending the entire oil chain, since it is possible to mention the Brazilian market updates on this context. To support this study, the historical development of the Brazilian petroleum industry was presented and the main stages of the production process were exposed with an emphasis on exploration and production phases. The new technologies developed for deepwater extractions, such as the pre-salt regions, were also discussed and, in the end, the relation of this growing market with the opportunities of the Human Resources Program of n. 48 was highlighted. This program identifies the market's actual needs and it's scarce's areas with the purpose of building capacity and qualified workforces, since it is also aimed, in a national level, a self-sufficient human resources for the sector