Influência da luz LED na cultura celular de Mieloma múltiplo murino–NS1 in vitro

A monoclonal antibody (mAb) is an important tool in medical biotechnology and the production of biopharmaceuticals, especially for disease diagnosis and treatment of infections, because the antibodies have a significant advantage over chemical agents used in conventional therapies . The last thirty years the technology of production of monoclonal antibodies developed mainly the technique of obtaining in vitro, but also of their production is laborious, the cost is high. A major element of the high cost of production is the fact that the long-term culture consumes a large amount of imported inputs with high added value. A major contribution of this work is to promote cell growth more quickly and efficiently. Currently, a great race to discover new technologies and techniques to synthesize new antibodies and significantly increase the production of murine mAbs. New technologies such as laser and LED are innovations and widespread in modern life, so much so that its use has proliferated worldwide, primarily in the medical field. Recent studies show a series of results from the influence of the LED light in biological tissues such as: increasing the rate of cell proliferation, increased production rate of fibroblasts, increasing the rate of synthesis of RNA and DNA synthesis of ATP, etc. To assess the contribution of the LED in the culture of Myeloma NS1murino compared to the standard procedure. - NS1 cells were provided and followed the criteria of culture medium of the Laboratory of Cellular Engineering Center of Botucatu (POPs). The same amount of cells was grown in bottles of 25 cm2 polystyrene Tissue Culture Treated, specifically marked and kept in special medium RPMI 1640 Gibco BRL  supplemented with fetal bovine serum 10%, essential amino acids and non-essential, glucose, insulin and antibiotics. It was used in LEDs Cromatek wavelength of 630nm, 475nm and 530nm. The groups were... (Complete abstract click electronic access below)

Estudo e análise do risco e da viabilidade econômica do mercado cativo e do mercado livre de energia elétrica no terceiro ciclo tarifário

In 2004 two trading environment for contracts of purchase and of electricity energy were established, the Regulated Contracting Environment (RGE) and the Free Contracting Environment (FCE). In the first one, consumers can only buy their energy directly from local electricity distribution, and in the second one, consumers can choose their delivery, amount and type of energy that they will burn through bilateral contracts. Thus, before deciding to migrate to the FCE, it is necessary understanding the rules of marketing, the risk involved and the economic viability of the two markets so can determine which environment has more benefits to the consumer. This paper aims to offer tools to support takeover decision of potentially free costumers, who have the option to migrate to market in order to evaluate the benefits and disadvantages of each market. This paper has also considered the new rules of the third rate cycle, where consumers can opt for green tax. The methodology presented is based on calculations of spending with energy and the risk of flag in captive market and free market in one year

Comparação de custo e viabilidade técnica de obras com parede de concreto em relação a obras de alvenaria estrutural

Are being released in the construction market in Brazil ventures called Super 6. These businesses sell the promise of delivery of the apartment in six months after the launch of the venture. To meet this deadline are being deployed the constructive method of concrete walls using aluminum shapes. This system basically consists of pouring slabs and walls once, i.e. building up the wall shapes and slab joints. On the walls are used cloth, with reinforcements in vain and corners of walls and on these screens are tied the electrical boxes and conduits. For each tower is used the so-called system of half way, i.e. the system so it is sufficient to mount the Middle deck. Using a concrete which can be deformed in the next day you can lift one deck every two days with ready electric and hydraulicsystem, without having to tow the wall doing only minor fixes in the imperfections after concrete. With this system won an incredible speed in the construction of the structure reducing in almost one-third the length of the work. This work aims to compare in terms of cost-benefit of masonry structural systems and this new concrete wall system called Super 6. For this comparison will be used as parameter values used for the achievement of the Enterprise Portal of Roses of constructor Tenda which is one of the first to use concrete wall system. This project basically consists of seven towers of six floors each and will be budgeted the cost of this project if it were held in structural masonry. From these data it will be possible to make a comparison about the actual beneficial to adopt this system

Avaliação da proliferação celular na próstata de gerbilos vasectomizados, após indução química pela injeção intraperitoneal do carcinógeno N-metil-N-nitrosuréia (MNU)

Prostate cancer is the second leading cause of cancer death in the country. Due to this factor, the interest of showing what relationship exists between vasectomy surgery and the incidence of prostate carcinoma has started. Some epidemiological studies showed an increased risk of prostate cancer in vasectomized men (Emard et al., 2001). On the other hand other authors have argued that there is no correlation (Patel et al., 2005) and there are those who said that vasectomy is linked to reduced risk of prostate cancer (Ross, 1983). Faced with an analysis of published works, such discussion remains today. The vasectomy, or deferentectomia, is a contraceptive male method, which is the section of the vas deferens of man by preventing the sperm from being expelled along with the seminal fluid during ejaculation, and is one of the most simple, economical, uncomplicated post- operative. This study aims to evaluate the process of cell proliferation and to evaluate immuno-histochemically the expression of specific markers of PCNA (Proliferating Cell Nuclear Antigen) and Ki-67, in the prostate of the gerbil after chemical induction by intraperitoneal injection of the carcinogen N-methyl -N-nitrosourea (MNU), because the regulation of the functional balance between cell proliferation and apoptosis is associated with hyperplasia and prostatic carcinoma. The experimental procedure was performed at the Laboratory of the Department of Anatomy, along with collecting the data for later analysis

Interferência eletromagnética em equipamentos de diatermia por microondas por aparelhos telefonia móvel celular GSM

Não disponível

Proliferação e Morte Celular (Apoptose) avaliadas em ratos Lewis após tratamento com mistura de praguicidas em baixas doses

The use of pesticides in Brazil has intensified over the years. And since 2009 it was ranked as the largest market for pesticides worldwide. The consequential diffuse contamination of the environment is therefore due to the increasing number of chemicals of different classes, origins and modes of action. Little is known about the action of pesticides on human health in situations of co-exposure. Possible toxic effects are not restricted to agricultural and industrial workers, but also the general population that may be exposed continuously to its residues in food and water. Although these pesticides are mostly present in the environment at low doses, it must be considered that possible cumulative or synergistic effects may occur when there are concurrent or sustained exposure for two or more of these agents, which can lead to late manifestation of subclinical damages, sometimes irreversible. Thus, the specific objective of this study was to assess the effect of carcinogenesis promotion of a mixture of pesticides at low doses and analyze the phenomena of cell proliferation and apoptosis in rat liver. A total of 50 male Lewis rats was separated into 5 groups for 8 weeks in a medium term hepatocarcinogenesis model. The three different classes of pesticides (dieldrin, dicofol, endosulfan, dichlorvos and permethrin), whose residues were detected by ANVISA during the period from 2001 to 2005 in tomatoes cultures, were added to the feed of rats initiated to hepatocarcinogenesis with diethylnitrosamine (DEN- 200mg/kg ip). We used two different mixtures, one with no toxic effects at doses (MEX1) referring to the NOEL (no-observed-effect level) and another at doses LOEL / LEL / LOAEL (Lowest-observed-effect level / Lowest-effect level / Lowest -observed-adverse-effect level), to the installation of adverse effects (MEX2), derived from chronic studies. All animals ...(Complete abstract click electronic access below)

Avaliação da viabilidade econômica da implantação de uma usina de triagem e compostagem na cidade de Rio Claro-SP

The question of the solid waste is without a doubt one of the biggest problems faced for the world-wide population in the present time, resulting in the call “collapse of the garbage”. In this direction that appears the composting, in the scope of the treatment of organic waste origin, that in Brazil, approximately correspond 50% of the generated volume. However, the rare plants of selection and composting (UTC) existing at least do not obtain auto-to support themselves, increasing to each day the number of deactivations, almost always related the financial questions. In this direction, the present research brings the analysis of the economic viability of the implantation of a UTC for the city of Rio Claro-SP, relating it with the thick organic composition sales, for the cultures of vegetables cultivated in a ray of 30 km of the study area. For in such a way, they had been carried through, calculations concerning the regional demand for organic composition, and the verification of the implantation costs and operation of the plant. The reached results had been the flow of box for the first year of functioning of the UTC, as well as the break-even point between the gotten expenditures and prescriptions. From this relation investment profitability, it was verified that the UTC is presented impracticable under the economic point of view.

Estudo da viabilidade técnica/econômica no aumento da eficiência energética sustentável aplicada à residência unifamiliar

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Análise da viabilidade econômica na implantação de inversores frequência em sistemas de bombeamento

The present work aims to conduct a study of the economic feasibility of the implementation of variable frequency inverters in pump systems with variable flow rates. The concern with the reduction of energy expenditure in industries raised in 2001 due to the energy crisis that hit Brazil at that time, forcing industries to reduce their electric costs under penalty of fines if this were not done (MOREIRA, 2008). Frequency inverter is an electronic device that allow greater control in the operation of pumping systems and also a reduction in electrical consumption, being a viable solution to achieve a reduction in energy consumption. For the development of this work, approaching important topics in pumping systems was necessary in order to make a correct choice of hydraulic pumping and also other components responsible for the operation of the system , such as electric motors and frequency inverters. In the case study, a theoretical analysis of the behavior of electrical consumption in systems with variable flow rates was performed, comparing when the system is operated via frequency inverters and when the system is operated without such a device. As expected, the result of the implantation of the device was quite satisfactory, and the primary goal of reducing energy expenditure was achieved. Also important to highlight the environmental issue of this work, as all forms of energy production affects nature in some way, achieving a reduction in consumption also contributes indirectly in environmental conservation

Análise de viabilidade da utilização do sorgo sacarino como cultura complementar na entressafra da cana-de-açucar

The sorghum is seen as a complementary option ethanol production in the offseason cane sugar because of its high energy potential and its culture to adapt well to hot climates and dry. This study evaluates the use of planting sweet sorghum as a culture supplement cane sugar in its offseason, leading to findings that show the feasibility of planting sorghum harvests of sugar cane

Viabilidade técnica e econômica na utilização de um sistema fotovoltaico para alimentação de luminárias LED na Faculdade de Engenharia do Campus de Guaratinguetá SP

Solar energy can be considered the largest source of energy available on earth and has attracted in recent decades, attention and interest for its rational use. The use of energy sources in a sustainable way is essential to the survival of future generations, due to the scarcity of natural resources and their exploitation in a disorderly way. This academic work aims to bring a possible alternative to reduce the consumption of eletrical energy, at the University of Engineering – Campus of Guaratinguetá, through the use of a photovoltaic system interconnected to the LED bulbs. For this, the necessary data for the design of the photovoltaic system componentes and their acquisition costs were obtained and three differents configurations were developed to choose the one that bests fits the studied case, considering the return time of the initial investment (payback)

Viabilidade do aproveitamento de resíduos de madeira para produção de móveis

The need to use waste from wood processing industry grows due to environmental demands imposed by laws, so like the need to introduce more competitive products in the market (it means better quality and acceptable cost) who must submit by the rational use of raw material, usually turning waste to sub products, what adds higher value for that. To this research, specific case from Ribon Company, located on Camaçari, Bahia-Brazil, has been studied. It is a treated wood producer. Bulk of monthly wood logs there is 70 m3. Its yield is 71,43%, where bulk of generated waste is monthly 20m3. As an exploitation way from generated waste, it has been suggested some options like: erosion protection to specific areas, a wildlife shelter as an henhouse etc, recreational articles, coal, linings, treadmills. However this research will give focus to the furniture design made of waste. As a conclusion, there is very much need to assign new utilizations to that sub used materials, since there is significant amount to increase company’s productive yield

Influência do receptor TLR-2 no processo de fagocitose do fungo Sporothrix schenckii

A esporotricose é uma micose provocada pelo fungo Sporothrix schenckii, resultante da inoculação direta de conídios e hifas do fungo. A doença se desenvolve habitualmente na pele, no tecido subcutâneo, podendo através dos vasos linfáticos, comprometer órgãos internos. Nos últimos anos tem-se voltado à atenção à participação do receptor Toll Like-2 no reconhecimento e fagocitose de diversos antígenos fungicos. A partir disto, o objetivo deste estudo foi estudar a influência do receptor Toll Like-2 no processo de fagocitose do Sporothrix schenckii em camundongos C57BL/6. Este estudo avaliou o processo de internalização do Sporothrix schenckii por macrófagos murinos obtidos de camundongos C57BL/6 WT e C57BL/6 TLR-2 KO. Os experimentos foram realizados através da utilização de lâminas de cultura celular e microscopia ótica. Além de analisar a viabilidade de células fúngicas internalizadas através de ensaios de sobrevivência. Como resultados pudemos observar a significativa importância do TLR-2 no processo de internalização deste microrganismo, uma vez que o estudo evidenciou uma eficácia de internalização acima de 85% dos macrófagos obtidos de animas selvagens em relação aos obtidos de animais TLR-2 KO. Além disso, através de ensaios de sobrevivência foram obtidos resultados que sugerem a ausência de viabilidade das células fúngicas depois de fagocitadas

Análise da viabilidade de simulações com feixes de prótons de 100, 150, 200 e 250 MeV em alvos heterogêneos

In the recent years, the use of proton beams in radiotherapy has been an outstanding progress (SMITH, 2006). Up to now, computed tomography (CT) is a prerequisite for treatment planning in this kind of therapy because it provides the electron density distribution required for calculation of dose and the interval of doses. However, the use of CT images for proton treatment planning ignores fundamental differences in physical interaction processes between photons and protons and is, therefore, potentially inaccurate (SADROZINSKI, 2004). Proton CT (pCT) can in principle directly measure the density distribution needed in a patient for the dose distribution (SCHULTE, et al, 2004). One important problem that should be solved is the implementation of image reconstruction algorithms. In this sense, it is necessary to know how the presence of materials with different density and composition interfere in the energy deposition by ionization and coulomb excitation, during its trajectory. The study was conducted in two stages, was used in both the program SRIM (The Stopping and Range of Ions in Matter) to perform simulations of the interaction of proton beams with pencil beam type. In the first step we used the energies in the range of 100-250 MeV (ZIEGLER, 1999). The targets were set to 50 mm in length for the beam of 100 MeV, due to its interaction with the target, and short-range, and 70 mm for 150, 200 and 250 MeV The target was composed of liquid water and a layer of 6 mm cortical bone (ICRP). It were made 9 simulations varying the position of the heterogeneity of 5 mm. In the second step the energy of 250 MeV was taken out from the simulations, due to its greater energy and less interaction. The targets were diminished to 50 mm thick to standardize the simulations. The layer of bone was divided into two equal parts and both were put in the ends of the target... (Complete abstract click electronic access below)

Utilização de técnicas de planejamento de experimentos na análise de viabilidade de alterações no processo de pintura de grades Antracite em uma indústria automotiva

The search for better performance and competitive advantage in the current market scenario has made organizations keep their focus on quality planning and the use of effective techniques aimed at continuous improvement and optimization of processes and resources. The situation is not different for the painting process in the automotive industry, which has a significant amount of variables and influential external factors. This work, developed at General Motors in São José dos Campos, aims to analyze the feasibility of changing the painting process of the pickup S10 railings in the Antracite color located in the main line to the another line located in a Painting Repair Booth at the plant and, therefore, to study the possibility about reducing the window of cure for Antracite’s enamel by using Design of Experiments’ techniques. Applying this method is possible to find the best arrangement among studied factors of Painting Repair Booth that are, in this case, the Temperature, in Celsius degrees, and Skid’s Steps, in seconds, and it’s also possible to get their behavior according to quality requirements specified by GM rules for the Brightness and Paint Adhesion on the part. As a result, it can conclude that is possible to paint the S10 Antracite railings in the painting process’ line of Painting Repair Booth, and with the new configuration obtained by the Design of Experiments for the process variables, there are significant gains related to the parts’ quality, costs reduction and the optimization of production capacity, much in the main line as in the line of the Painting Repair Booth