Investigação da expressão gênica diferencial em resposta aos efeitos da proteína anti-inflamatória Anexina A1 nas células de carcinoma de colo de útero

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Embriões bovinos produzidos in vitro com espermatozoides sexados por citometria de fluxo: avaliação do desenvolvimento embrionário e da expressão de genes candidatos ao reconhecimento da gestação

Pós-graduação em Genética e Melhoramento Animal - FCAV

Entre a expressão e a técnica: a terminologia do professor de canto - um estudo de caso em pedagogia vocal de canto erudito e popular no eixo Rio-São Paulo

Pós-graduação em Música - IA

Análise da expressão das proteínas celulares p53, p16 e ciclina D1 em amostras de tumores penianos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Expressão de fatores ligados à apoptose : herpesvirus bovino tipo 5

Pós-graduação em Microbiologia - IBILCE

Expressão e imunidade das Heat Shock Proteins (HSP) em gestantes portadoras de diabete e hiperglicemia gestacional leve

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Estudo comparativo de dois programas de computador (Dolphin Imaging e Nemotec) versus o método manual utilizados para análises cefalométricas de pacientes com deformidade dento-facial padrão II

A-Nperp, Pg-Nperp e mandibular plane). Thirty patients (male or female), with age varying from 19 to 48 years old were analyzed. All patients were diagnosted with mandibular retrognathism and submitted to bilateral sagital mandibular advancement. All tracings were performed in lateral cephalometric radiographs obtained one week before surgery. The intraclass correlation coefficient analysis (ICC), the t test adjusted for Tukey-Kramer test and Schuirmann test were applied. The results showed that the manual tracings and Nemotec® tracing had excellent reliabilities for all measures (ICC > 0.98). The Dolphin Imaging® showed low reliability in anterior facial height (value of ICC = 0.70), Co-A (value of ICC = 0.47) and Co-Gn (ICC value = 0.49). In A-Nperp, Pg-Nperp, Mandibular plan, SNA and SNB there were no differences between the 3 tracings (p > 0.05), for the anterior facial height measures differences were found between the Dolphin Imaging® and Nemotec® tracings, but no differences were observed as compared to the manual tracing (p > 0.05), in Co-A and Co-Gn measures t Dolphin Imaging® presented a significantly lower mean than the other methods (p > 0.05). The manual tracings were equivalent in 6 of 8 measures (A-Nperp, Pg-Nperp, md Plan, SNA, SNB and Co-A), there was no equivalence between the methods in the anterior facial height and Co-Gn measures (p < 0.01). The Dolphin Imaging® method was not equivalent in any of the 8 measures. It was concluded that in the manual tracing only the Co- Gn, Pg-Nperp and SNB measures confirmed the diagnosis of mandibular retrognathism, and the Nemotec® software showed better results than the Dolphin Imaging® software.

Formação de leitores surdos e a educação inclusiva

Pós-graduação em Educação - FFC

Efeitos do treinamento físico aeróbico sobre a expressão da miostatina e o trofismo de músculos esqueléticos e cardíaco de ratos espontaneamente hipertensos com insuficiência cardíaca

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação óptica, ultraestrutural e expressão de proliferação celular da mucosa entérica de frangos de corte suplementados com glutamina e ácido glutâmico

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do efeito da lectina Artin M na expressão de fatores de crescimento e citocinas inflamatórias relacionadas ao processo de repação tecidual: Estudo in vitro em macrófagos e fibroblastos gengivais de ratos

Pós-graduação em Odontologia - FOAR

Estudo imuno-histoquímico da co-expressão de citoqueratinas, vimentina e actina em neoplasias de glândula salivar

Human salivary gland tumors originated from intercalated ducts present a broad range of histologic and cytologic patterns, mainly due to the presence of myoepithelial cells. The aim of this study is to verify the differentiation grade of neoplastic cells and a possible relation between myoepithelial cell differentiation and the presence of luminal secretory contents. The expression of vimentin and cytokeratin (CK) intermediate filaments, actin myofilament and epithelial membrane antigen (EMA) was investigated by double labeling immunocytochemical technique, in thirty salivary gland neoplasms: 5 pleomorphic adenomas, 5 myoepitheliomas, 3 basal cell adenomas, 7 adenoid cystic carcinomas (ACC) and 10 polimorphous low grade adenocarcinomas (PLGA). Tumors with intercalated duct differentiation (pleomorphic adenomas, basal cell adenomas and ACC) express CKs 7, 8, 18 and 19 in the luminal cells and coexpress eventually CK14 with these CKs. Some luminal cells stained with anti-EMA antibody, mainly where a secretory content in the lumen was observed. Outer ductal cells and other myoepithelial-like cells express vimentin, sometimes coexpressing actin and/or CK14 with vimentin. Plasmacytoid cells in myoepitheliomas and pleomorphic adenomas express vimentin and rarely CKs 7, 8, 18 and 19, sometimes coexpressing these CKs with CK14 but they are negative for the remaining antigens. Tumors without intercalated duct differentiation (solid basal cell adenoma and PLGA) express vimentin and CKs 7, 8, 14 and 18, sometimes coexpressing CKs 8 and 18 with CK14. In conclusion, in tumors with intercalated duct differentiation, myoepithelial cells express vimentin and sometimes coexpress actin and/or CK14 with vimentin, never coexpressing other CKs with vimentin. CK14 and actin are independently expressed by myoepithelial cells, so their expression is probably induced by different stimulus. However, the secretory function of luminal cells, visualized by EMA staining, ....

Expressão da proteína nuclear de proliferação celular (PCNA), da B-Catenina e do Agnor, na etapa inicial do processo de carcinogênese quimicamente induzido pelo DMBA, em borda lateral de língua de hamsters sírio dourado(Mesocricetus auratus)

The oral cancer model in hamsters shows many simílarities with developmental oral cancer in humans. The proliferating capacity is one the most characteristics of neoplásica ce/Is and detection of these ce/Is allow us, throughout of its counting, to achieve an estimated tumour growing index, with a consequent repercussion about prognostic and in the treatment of those lesions. 40 golden Syrian hamsters, both genders, aged between two to six mouth and weight 150g in average were used. The left síde of tangue of each animal was painted for eight consecutive weeks, with a solution of DMBA. Tongues were removed, fixed in 10% buffered fonnal solution. The histological slides were stained by AgNOR technique and by PCNA and β-catenin immunohistochemical antibodies. Statistical analyzes were performed by ANOVA one-way test and Tukey test. We may conclude that an association between AgNOR and PCNA might indicate the higher proliferating activity of the analyzed celIs. The experimental carcinogenesis model in hamster tongue is an available methodology for immunohistochemistry study. And finally, PCNA and β-catenin immunohistochemical antibodies may be used to analyze possible premalignant areas in oral leukoplakia

Avaliação da expressão de citoqueratinas na citologia esfoliativa da mucosa bucal de pacientes fumantes

The present study compared the expression of cytokeratins CK6, CK16 and CK19 and pan-cytokeratin (PAN) in oral mucosa cells between smokers and nonsmokers in order to determine the stage of cell differentiation and to consequently infer proliferative activity and expressions indicative of a potential for malignant differentiation. Thirty smokers and 30 non-smokers seen at the clinics of FOSJC-UNESP were screened. Smears were obtained from the left lateral border of the tongue with a cytobrush and slides were processed for immunohistochemistry using the antibodies reported Conventional microscopy was used for qualitative analysis. The results were analyzed statistically by the Z test, Fisher's exact test and comparison of two proportions (plus-4 confidence interval method). The expression of CK6 (p=0.002), CK16 (p=0.003), CK19 (p=0.0001) and PAN (p=0.008) was higher in oral mucosa smears from smokers compared to non- smokers. ln conclusion, increased epithelial proliferation is observed in the oral mucosa of smokers as demonstrated by the increased expression of CK6 and CKJ6, and these cells present alterations in epithelial maturation

Desempenho, características de carcaça e expressão de genes em tourinhos alimentados com lipídeos e monensina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)