Identification of blood meal sources of Lutzomyia longipalpis using polymerase chain reaction-restriction fragment length polymorphism analysis of the cytochrome B gene

An analysis of the dietary content of haematophagous insects can provide important information about the transmission networks of certain zoonoses. The present study evaluated the potential of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the mitochondrial cytochrome B (cytb) gene to differentiate between vertebrate species that were identified as possible sources of sandfly meals. The complete cytb gene sequences of 11 vertebrate species available in the National Center for Biotechnology Information database were digested with Aci I, Alu I, Hae III and Rsa I restriction enzymes in silico using Restriction Mapper software. The cytb gene fragment (358 bp) was amplified from tissue samples of vertebrate species and the dietary contents of sandflies and digested with restriction enzymes. Vertebrate species presented a restriction fragment profile that differed from that of other species, with the exception of Canis familiaris and Cerdocyon thous. The 358 bp fragment was identified in 76 sandflies. Of these, 10 were evaluated using the restriction enzymes and the food sources were predicted for four: Homo sapiens (1), Bos taurus (1) and Equus caballus (2). Thus, the PCR-RFLP technique could be a potential method for identifying the food sources of arthropods. However, some points must be clarified regarding the applicability of the method, such as the extent of DNA degradation through intestinal digestion, the potential for multiple sources of blood meals and the need for greater knowledge regarding intraspecific variations in mtDNA.

Gestão, mediação e uso da informação

O livro Gestão, mediação e uso da informação vai ao encontro dos estudos teóricos e metodológicos de objetos e fenômenos que envolvem a gestão, a mediação, uso e apropriação da informação em distintos ambientes

Serologic survey of West Nile virus in horses from Central-West, Northeast and Southeast Brazil

Processo FAPESP: 08/50167-6

Craniocervical posture and craniofacial morphology

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Swimming Exercise Changes Hemodynamic Responses Evoked by Blockade of Excitatory Amino Receptors in the Rostral Ventrolateral Medulla in Spontaneously Hypertensive Rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of Ocimum basilicum Linn Essential Oil and Sodium Hexametaphosphate on the Shelf Life of Fresh Chicken Sausage

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ability of Salmonella spp. to Produce Biofilm Is Dependent on Temperature and Surface Material

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of metal-biomass interactions in the lanthanum(III) biosorption on Sargassum sp using SEM/EDX, FTIR, and XPS: Preliminary studies

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

One-sided and two-sided Green's functions

We discuss the one-sided Green's function, associated with an initial value problem and the two-sided Green's function related to a boundary value problem. We present a specific calculation associated with a differential equation with constant coefficients. For both problems, we also present the Laplace integral transform as another methodology to calculate these Green's functions and conclude which is the most convenient one. An incursion in the so-called fractional Green's function is also presented. As an example, we discuss the isotropic harmonic oscillator.

Frailty in older adults: perspectives for research and practice in Public Health

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Letter to the Editors regarding the article published in volume 30, number 3

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Correlates of above-average cognitive performance among older adults: the SABE study

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characteristics of physical activity programs in the Brazilian primary health care system

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Absenteísmo por doença ocupacional de trabalhadores rurais no setor canavieiro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

BASTA APLICAR UMA INJEÇÃO? DESAFIOS E CONTRADIÇÕES DA SAÚDE PÚBLICA NOS TEMPOS DE JK (1956-1961). Muniz ES. Belo Horizonte: Fino Traço/Campina Grande: EDUEPB/Rio de Janeiro: Editora Fiocruz; 2013. 104 p.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)